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The map label for this gene is hypB [H]

Identifier: 126208791

GI number: 126208791

Start: 1522186

End: 1522995

Strand: Direct

Name: hypB [H]

Synonym: APL_1327

Alternate gene names: 126208791

Gene position: 1522186-1522995 (Clockwise)

Preceding gene: 126208790

Following gene: 126208792

Centisome position: 66.92

GC content: 41.73

Gene sequence:

>810_bases
ATGTGTTCGACTTGCGGATGCGGTAGTGCTCAAGTAAGAATCGGAGAATTGCCGCACACTCATTCTAATGATGGAGAAAT
TCCCTCAAAACCTCAACATTCCAATCCTTTCGTTATCAACGGCATTGCCGAAATACAAAAACCGGATACGGCAAACGTTC
AAACTCGCTTACTTAAAATCGAACAGGACGTATTAGGCGAAAACAATCAATATGCACGTTTAAATCGTGATTTCTTTAAA
CAGAACAATATCTTGGCGTTAAATTTAGTTTCCAGCCCCGGTTCCGGTAAAACGACATTACTCGCTTCTACACTCACTTC
TTTGAAAGAACGTAAGCAATGTTATGTGATTGAAGGCGATCAGCAAACCGAAAACGATGCGAATCGAATCAGAGCGACGG
GAGTGCCGGCATTACAAATTAATACCGGCAAAGGCTGTCACTTGGATGCCAAAATGGTGTTAGAAGCGTTAGCAAAACTG
CAACCGAATCCGAACAGTACCGTATTTATCGAAAATGTCGGCAACTTGGTATGTCCTGCCGAATTTGATTTAGGCGAGTT
TGCGAAAGTAGCGATTCTCTCGGTAACGGAAGGCGAAGATAAGCCGCTGAAATATCCGCATATGTTTATGGCGGCTAAAT
TAATGATTATTAATAAAATAGATCTCTTACCTTACCTCAACTTCGATTTGGAAAAATGTATTGCCTATGCAAAACAAGTG
AATCCGAATATTCAAATCTTTTGTCTTTCAGCCACGTCCGGTGAAGGTATGGAAACGTGGTTGGACTGGTTGGAAAGTCA
GACGGCTTAA

Upstream 100 bases:

>100_bases
GCTATTTTATGGGTATTGTGATCCTGATCAACTTAGCCGAATTGAAAGTATAGTCTAATCTCTATAATGATCATCATACC
TTGTAGTTAGGGAGATAAAT

Downstream 100 bases:

>100_bases
GGAATTATAAAATGCAATTTGTCGATGAATTTAGAGATCCCGTTCTGGCTCGTAAATTAATTACGCATTTACAGCAATTA
ATGCAAAAGTTGCCGCAATT

Product: hydrogenase nickel incorporation protein HypB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MCSTCGCGSAQVRIGELPHTHSNDGEIPSKPQHSNPFVINGIAEIQKPDTANVQTRLLKIEQDVLGENNQYARLNRDFFK
QNNILALNLVSSPGSGKTTLLASTLTSLKERKQCYVIEGDQQTENDANRIRATGVPALQINTGKGCHLDAKMVLEALAKL
QPNPNSTVFIENVGNLVCPAEFDLGEFAKVAILSVTEGEDKPLKYPHMFMAAKLMIINKIDLLPYLNFDLEKCIAYAKQV
NPNIQIFCLSATSGEGMETWLDWLESQTA

Sequences:

>Translated_269_residues
MCSTCGCGSAQVRIGELPHTHSNDGEIPSKPQHSNPFVINGIAEIQKPDTANVQTRLLKIEQDVLGENNQYARLNRDFFK
QNNILALNLVSSPGSGKTTLLASTLTSLKERKQCYVIEGDQQTENDANRIRATGVPALQINTGKGCHLDAKMVLEALAKL
QPNPNSTVFIENVGNLVCPAEFDLGEFAKVAILSVTEGEDKPLKYPHMFMAAKLMIINKIDLLPYLNFDLEKCIAYAKQV
NPNIQIFCLSATSGEGMETWLDWLESQTA
>Mature_269_residues
MCSTCGCGSAQVRIGELPHTHSNDGEIPSKPQHSNPFVINGIAEIQKPDTANVQTRLLKIEQDVLGENNQYARLNRDFFK
QNNILALNLVSSPGSGKTTLLASTLTSLKERKQCYVIEGDQQTENDANRIRATGVPALQINTGKGCHLDAKMVLEALAKL
QPNPNSTVFIENVGNLVCPAEFDLGEFAKVAILSVTEGEDKPLKYPHMFMAAKLMIINKIDLLPYLNFDLEKCIAYAKQV
NPNIQIFCLSATSGEGMETWLDWLESQTA

Specific function: Required for the maturation of the three [NiFe]hydrogenases. Exhibits a low intrinsic GTPase activity. The GTP hydrolysis catalyzed by HypB is an integral process in the incorporation of nickel into hydrogenases [H]

COG id: COG0378

COG function: function code OK; Ni2+-binding GTPase involved in regulation of expression and maturation of urease and hydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypB/hupM family [H]

Homologues:

Organism=Escherichia coli, GI1789082, Length=289, Percent_Identity=56.401384083045, Blast_Score=313, Evalue=6e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003495
- InterPro:   IPR004392
- InterPro:   IPR012202 [H]

Pfam domain/function: PF02492 cobW [H]

EC number: NA

Molecular weight: Translated: 29577; Mature: 29577

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSTCGCGSAQVRIGELPHTHSNDGEIPSKPQHSNPFVINGIAEIQKPDTANVQTRLLKI
CCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEECHHHCCCCCCCCHHHHHHHH
EQDVLGENNQYARLNRDFFKQNNILALNLVSSPGSGKTTLLASTLTSLKERKQCYVIEGD
HHHHCCCCCCEEECCHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEECC
QQTENDANRIRATGVPALQINTGKGCHLDAKMVLEALAKLQPNPNSTVFIENVGNLVCPA
CCCCCHHHHEEECCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCEECCC
EFDLGEFAKVAILSVTEGEDKPLKYPHMFMAAKLMIINKIDLLPYLNFDLEKCIAYAKQV
CCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHC
NPNIQIFCLSATSGEGMETWLDWLESQTA
CCCEEEEEEECCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MCSTCGCGSAQVRIGELPHTHSNDGEIPSKPQHSNPFVINGIAEIQKPDTANVQTRLLKI
CCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEECHHHCCCCCCCCHHHHHHHH
EQDVLGENNQYARLNRDFFKQNNILALNLVSSPGSGKTTLLASTLTSLKERKQCYVIEGD
HHHHCCCCCCEEECCHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEECC
QQTENDANRIRATGVPALQINTGKGCHLDAKMVLEALAKLQPNPNSTVFIENVGNLVCPA
CCCCCHHHHEEECCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCEECCC
EFDLGEFAKVAILSVTEGEDKPLKYPHMFMAAKLMIINKIDLLPYLNFDLEKCIAYAKQV
CCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHC
NPNIQIFCLSATSGEGMETWLDWLESQTA
CCCEEEEEEECCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]