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The map label for this gene is ligA

Identifier: 126208768

GI number: 126208768

Start: 1492112

End: 1494163

Strand: Reverse

Name: ligA

Synonym: APL_1302

Alternate gene names: 126208768

Gene position: 1494163-1492112 (Counterclockwise)

Preceding gene: 126208769

Following gene: 126208767

Centisome position: 65.69

GC content: 44.88

Gene sequence:

>2052_bases
ATGCCTTTATTATCACAAATGCAAGAATACCAAGCGGACACGACTTTCGCACAATTAGAAGCTTTACGTCAAAAATTACG
AGAATATGAATACCATTATCACGTATTGGATAATCCGTTAGTGCCGGATGCGGAATATGACCGCTTAATGAATGAATTAA
AAAATCTGGAATGGCAACATCCGGAATGGATTACCGTCGATTCTCCGACCCAACGTGTCGGGGCGAAACCGCTGGACGGC
TTTGCACAAGTGACGCACGAAATTCCGATGTTATCGTTGGATAATGCCTTTTCCGATGAAGAATTAGACGGTTTTTTACG
CCGTATGGAAAGTTATATTACTGAAGATCCGCATACGCTTGCTTTTTGTTGCGAACCTAAATTAGACGGTTTAGCGGTCA
GTATTTTGTATGTGGATGGAGTGTTAAGCCAAGCGGCAACCCGTGGTGACGGTAGCACCGGCGAGGATATTACCTCGAAT
ATTCGTACCATTCGTAATATCCCGTTAAAACTGAATATGGATAATCCGCCGGCACGCTTAGAAGTGCGTGGCGAGGTGTT
TATGCCGCAAAAGGGCTTCGAAGAATTGAATGAACGTGCGTTGGAAAAAGGTGAGAAAACCTTTGCTAACCCACGTAATG
CGGCGGCAGGCTCATTACGTCAGTTAGATCCGAAAATCACTCGTCAGCGTCCGCTTGTGTTAAATGCCTATGGAATCGGT
GTTTATGAATCGGATGATGAATTACCTGCAACCCATTTTGAACGTTTGCAATGGCTGAAATCTATCGGTATTCCGGTTAA
TAATGAAATTCGCTTGGCAACAGGGCGTGAGCAATTACTTGCGTTCTATGCGAATATTCAAGCCAAGCGTCCGACACTCG
GTTATGACATTGACGGCACGGTGCTAAAAGTGAATGACATCGGCTTACAAGAGCAGCTCGGCTTTATTTCTCGTTCGCCG
CGTTGGGCGATTGCTTATAAGTTTCCGTCACAAGAAGAAATGACGGTGTTGAATGATGTGGAATTTCAGGTGGGCAGAAC
CGGGGCAATTACCCCAGTCGCAAAATTAGAACCGGTATTCGTTGCCGGTGTGACGGTGAGTAATGCCACTTTACATAACG
GTGATGAAATTGAGCGTTTAGGCATTGTCATCGGCGATACGGTGATTATTCGCCGTGCCGGTGATGTGATTCCGCAAATT
GTCGGGGTGGTGATGGATCGCCGTCCGGAAAATGCAAAAAAAATCGAATTTCCGACTGCTTGTCCGGTATGTGAATCGGC
GGTAGTACGTGTGGAAGGCGAAGCGGTTGCTCGCTGTACCGGCGGTTTATTCTGTGGTGCACAGCGTAAAGAAGCGTTGA
AGCATTTCGTTTCCCGTAAAGCAATGGATATTGACGGGGTCGGCGAAAAATTGATTGAGCAATTAATGGAACGTGAGCTG
GTGCATACTCCTGCGGATCTGTTTAAACTCGATCACACAACTTTAATGCGTTTGGAACGAATGGGCGAAAAATCGGCACA
AAATGCGTTAAACAGCATCGAAAAAGCAAAAAATACCACTTTGGCACGTTTCCTATTTGCATTAGGTATTCGTGATGTCG
GTGAAGCTACCGCACAAAACTTAGCGAACCATTTCCATAATTTGGCAGCGATTCGTACGGCAACTTTCGAGCAATTACAA
GCGGTACAGGATGTCGGTGAAGTGGTGGCAAATCGTATTGTACGTTTCTGGCAAGAACCTCATAATGTTGCGGTTGTCGA
AGATTTGATTTCACAAGGCGTACATTGGCAAGATGTGATTCAGGTTGAAATTGCGGATAACCCGTTAAAAGGTAAAAATG
TGGTTTTAACCGGTACACTTACTCAATTAACCCGAGATCAAGCAAAAGCATTGCTACAGAGTTTCGGTTGTAAAGTATCC
GGCTCGGTTTCAAGCAAAACCGATTATTTGATTGCCGGTGAAAAAGCCGGTTCAAAATTGGCAAAAGCGCAAGAACTTGG
CGTTAAGATATTGACCGAACAAGAATTTATCGCTTTAACCGGCGAAAATTAA

Upstream 100 bases:

>100_bases
GAACTCACGCCAAGCCTATTTAGCTCGAGTGAGCTAATTTGCAAAACTTTTAGCATAAACGACCGCTTGTTAAGCGGTCT
TTTTTATCGAGAATTTCCTA

Downstream 100 bases:

>100_bases
ATTATCGTTTGTCAGCAAAAAAGCGTAAAATACAAGCGGTCGAATTTCTAAGAGGATTTACAAATGGCTATTTTAGTAAC
TGGTGGTGCAGGTTATATCG

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 683; Mature: 682

Protein sequence:

>683_residues
MPLLSQMQEYQADTTFAQLEALRQKLREYEYHYHVLDNPLVPDAEYDRLMNELKNLEWQHPEWITVDSPTQRVGAKPLDG
FAQVTHEIPMLSLDNAFSDEELDGFLRRMESYITEDPHTLAFCCEPKLDGLAVSILYVDGVLSQAATRGDGSTGEDITSN
IRTIRNIPLKLNMDNPPARLEVRGEVFMPQKGFEELNERALEKGEKTFANPRNAAAGSLRQLDPKITRQRPLVLNAYGIG
VYESDDELPATHFERLQWLKSIGIPVNNEIRLATGREQLLAFYANIQAKRPTLGYDIDGTVLKVNDIGLQEQLGFISRSP
RWAIAYKFPSQEEMTVLNDVEFQVGRTGAITPVAKLEPVFVAGVTVSNATLHNGDEIERLGIVIGDTVIIRRAGDVIPQI
VGVVMDRRPENAKKIEFPTACPVCESAVVRVEGEAVARCTGGLFCGAQRKEALKHFVSRKAMDIDGVGEKLIEQLMEREL
VHTPADLFKLDHTTLMRLERMGEKSAQNALNSIEKAKNTTLARFLFALGIRDVGEATAQNLANHFHNLAAIRTATFEQLQ
AVQDVGEVVANRIVRFWQEPHNVAVVEDLISQGVHWQDVIQVEIADNPLKGKNVVLTGTLTQLTRDQAKALLQSFGCKVS
GSVSSKTDYLIAGEKAGSKLAKAQELGVKILTEQEFIALTGEN

Sequences:

>Translated_683_residues
MPLLSQMQEYQADTTFAQLEALRQKLREYEYHYHVLDNPLVPDAEYDRLMNELKNLEWQHPEWITVDSPTQRVGAKPLDG
FAQVTHEIPMLSLDNAFSDEELDGFLRRMESYITEDPHTLAFCCEPKLDGLAVSILYVDGVLSQAATRGDGSTGEDITSN
IRTIRNIPLKLNMDNPPARLEVRGEVFMPQKGFEELNERALEKGEKTFANPRNAAAGSLRQLDPKITRQRPLVLNAYGIG
VYESDDELPATHFERLQWLKSIGIPVNNEIRLATGREQLLAFYANIQAKRPTLGYDIDGTVLKVNDIGLQEQLGFISRSP
RWAIAYKFPSQEEMTVLNDVEFQVGRTGAITPVAKLEPVFVAGVTVSNATLHNGDEIERLGIVIGDTVIIRRAGDVIPQI
VGVVMDRRPENAKKIEFPTACPVCESAVVRVEGEAVARCTGGLFCGAQRKEALKHFVSRKAMDIDGVGEKLIEQLMEREL
VHTPADLFKLDHTTLMRLERMGEKSAQNALNSIEKAKNTTLARFLFALGIRDVGEATAQNLANHFHNLAAIRTATFEQLQ
AVQDVGEVVANRIVRFWQEPHNVAVVEDLISQGVHWQDVIQVEIADNPLKGKNVVLTGTLTQLTRDQAKALLQSFGCKVS
GSVSSKTDYLIAGEKAGSKLAKAQELGVKILTEQEFIALTGEN
>Mature_682_residues
PLLSQMQEYQADTTFAQLEALRQKLREYEYHYHVLDNPLVPDAEYDRLMNELKNLEWQHPEWITVDSPTQRVGAKPLDGF
AQVTHEIPMLSLDNAFSDEELDGFLRRMESYITEDPHTLAFCCEPKLDGLAVSILYVDGVLSQAATRGDGSTGEDITSNI
RTIRNIPLKLNMDNPPARLEVRGEVFMPQKGFEELNERALEKGEKTFANPRNAAAGSLRQLDPKITRQRPLVLNAYGIGV
YESDDELPATHFERLQWLKSIGIPVNNEIRLATGREQLLAFYANIQAKRPTLGYDIDGTVLKVNDIGLQEQLGFISRSPR
WAIAYKFPSQEEMTVLNDVEFQVGRTGAITPVAKLEPVFVAGVTVSNATLHNGDEIERLGIVIGDTVIIRRAGDVIPQIV
GVVMDRRPENAKKIEFPTACPVCESAVVRVEGEAVARCTGGLFCGAQRKEALKHFVSRKAMDIDGVGEKLIEQLMERELV
HTPADLFKLDHTTLMRLERMGEKSAQNALNSIEKAKNTTLARFLFALGIRDVGEATAQNLANHFHNLAAIRTATFEQLQA
VQDVGEVVANRIVRFWQEPHNVAVVEDLISQGVHWQDVIQVEIADNPLKGKNVVLTGTLTQLTRDQAKALLQSFGCKVSG
SVSSKTDYLIAGEKAGSKLAKAQELGVKILTEQEFIALTGEN

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=666, Percent_Identity=63.3633633633634, Blast_Score=863, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=516, Percent_Identity=21.8992248062016, Blast_Score=104, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_ACTP2 (A3N1V2)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053993.1
- ProteinModelPortal:   A3N1V2
- STRING:   A3N1V2
- GeneID:   4849676
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1302
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   A3N1V2
- ProtClustDB:   PRK07956
- BioCyc:   APLE416269:APL_1302-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 75942; Mature: 75810

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 127-127 BINDING 125-125 BINDING 148-148 BINDING 185-185 BINDING 303-303 BINDING 327-327

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLLSQMQEYQADTTFAQLEALRQKLREYEYHYHVLDNPLVPDAEYDRLMNELKNLEWQH
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHCCCCCC
PEWITVDSPTQRVGAKPLDGFAQVTHEIPMLSLDNAFSDEELDGFLRRMESYITEDPHTL
CCEEEECCCHHHHCCCCCHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCEE
AFCCEPKLDGLAVSILYVDGVLSQAATRGDGSTGEDITSNIRTIRNIPLKLNMDNPPARL
EEEECCCCCCEEEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCEE
EVRGEVFMPQKGFEELNERALEKGEKTFANPRNAAAGSLRQLDPKITRQRPLVLNAYGIG
EECCEEECCCHHHHHHHHHHHHHCHHHHCCCCCHHHCCHHHCCCHHHCCCCEEEEEEECC
VYESDDELPATHFERLQWLKSIGIPVNNEIRLATGREQLLAFYANIQAKRPTLGYDIDGT
EECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCCC
VLKVNDIGLQEQLGFISRSPRWAIAYKFPSQEEMTVLNDVEFQVGRTGAITPVAKLEPVF
EEEEECCCHHHHHHHHCCCCCEEEEEECCCCCCCHHHCCCEEEECCCCCCCCHHHCCCEE
VAGVTVSNATLHNGDEIERLGIVIGDTVIIRRAGDVIPQIVGVVMDRRPENAKKIEFPTA
EEEEEEECCEECCCCHHHHHEEEECCEEEEEHHHHHHHHHHHHHHCCCCCCCCEEECCCC
CPVCESAVVRVEGEAVARCTGGLFCGAQRKEALKHFVSRKAMDIDGVGEKLIEQLMEREL
CCCHHHHEEEECCCHHHHHCCCEEECHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
VHTPADLFKLDHTTLMRLERMGEKSAQNALNSIEKAKNTTLARFLFALGIRDVGEATAQN
HCCCHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LANHFHNLAAIRTATFEQLQAVQDVGEVVANRIVRFWQEPHNVAVVEDLISQGVHWQDVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHEE
QVEIADNPLKGKNVVLTGTLTQLTRDQAKALLQSFGCKVSGSVSSKTDYLIAGEKAGSKL
EEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCEEECCCCCCCCEEEECCCCCHHH
AKAQELGVKILTEQEFIALTGEN
HHHHHCCEEEEECCEEEEEECCC
>Mature Secondary Structure 
PLLSQMQEYQADTTFAQLEALRQKLREYEYHYHVLDNPLVPDAEYDRLMNELKNLEWQH
CHHHHHHHHHCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHCCCCCC
PEWITVDSPTQRVGAKPLDGFAQVTHEIPMLSLDNAFSDEELDGFLRRMESYITEDPHTL
CCEEEECCCHHHHCCCCCHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCEE
AFCCEPKLDGLAVSILYVDGVLSQAATRGDGSTGEDITSNIRTIRNIPLKLNMDNPPARL
EEEECCCCCCEEEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCEE
EVRGEVFMPQKGFEELNERALEKGEKTFANPRNAAAGSLRQLDPKITRQRPLVLNAYGIG
EECCEEECCCHHHHHHHHHHHHHCHHHHCCCCCHHHCCHHHCCCHHHCCCCEEEEEEECC
VYESDDELPATHFERLQWLKSIGIPVNNEIRLATGREQLLAFYANIQAKRPTLGYDIDGT
EECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCCC
VLKVNDIGLQEQLGFISRSPRWAIAYKFPSQEEMTVLNDVEFQVGRTGAITPVAKLEPVF
EEEEECCCHHHHHHHHCCCCCEEEEEECCCCCCCHHHCCCEEEECCCCCCCCHHHCCCEE
VAGVTVSNATLHNGDEIERLGIVIGDTVIIRRAGDVIPQIVGVVMDRRPENAKKIEFPTA
EEEEEEECCEECCCCHHHHHEEEECCEEEEEHHHHHHHHHHHHHHCCCCCCCCEEECCCC
CPVCESAVVRVEGEAVARCTGGLFCGAQRKEALKHFVSRKAMDIDGVGEKLIEQLMEREL
CCCHHHHEEEECCCHHHHHCCCEEECHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
VHTPADLFKLDHTTLMRLERMGEKSAQNALNSIEKAKNTTLARFLFALGIRDVGEATAQN
HCCCHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LANHFHNLAAIRTATFEQLQAVQDVGEVVANRIVRFWQEPHNVAVVEDLISQGVHWQDVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHEE
QVEIADNPLKGKNVVLTGTLTQLTRDQAKALLQSFGCKVSGSVSSKTDYLIAGEKAGSKL
EEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCEEECCCCCCCCEEEECCCCCHHH
AKAQELGVKILTEQEFIALTGEN
HHHHHCCEEEEECCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA