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The map label for this gene is murA

Identifier: 126208752

GI number: 126208752

Start: 1477025

End: 1478299

Strand: Reverse

Name: murA

Synonym: APL_1286

Alternate gene names: 126208752

Gene position: 1478299-1477025 (Counterclockwise)

Preceding gene: 126208753

Following gene: 126208749

Centisome position: 64.99

GC content: 44.94

Gene sequence:

>1275_bases
ATGGAAAAATTTCGTGTTCACGGTCCTTTTACATTAAGCGGAACCGTTGATATTTCAGGTGCTAAAAATGCGGCACTCCC
GATTCTTTTTGCTGCCGTTTTGGCAACAGAACCTGTGACTTTAACTAATGTTCCTGATCTTAAAGATGTGGACACGACAT
TTAAAATCTTACGTAAATTAGGCGTAGTGGTTGAACGTGACGAAAGCGGCGCAGTACAAATTGATGCAAGTAAAATCGAT
CACTACGTTGCGCCTTACGAATTGGTCAAAACCATGCGTGCTTCGATCTGGGCATTAGCACCGCTTGTCGCTCGCTTCCA
CGAAGGGCAAGTTTCATTACCGGGCGGTTGTACGATTGGAGCTCGTCCCGTCGATATGCATATTTCCGGTTTAGAAAAAA
TGGGTGCGCTTATCGAATTGGATGAAGGTTATGTGAAGGCGACTTCAAACGGTCGTTTACACGGTGCGCGTATTTATATG
GATAAAGTCAGCGTAGGTGCGACATTATCGGTAATGATGGCGGCGACCTTAGCGGAAGGTACAACGACAATTGAAAATGC
GGCGCGTGAACCTGAAATCGTAGATACCGCATTATTCTTAAACGCAATGGGAGCAAAAATCTCTGGTGCGGGTACTGATA
CGATTACTATCGAAGGTGTGGAACGTTTAACCGGTTGCCAGCATCGTATCGTTGCCGACCGCATTGAAACCGGCACATTC
TTAGTCGCCGCAGCGGTATCCGGCGGTAAAATTACTTGTCGCGGAACCAAAGCGGATACTTTAGAAGCGGTAATTGAAAA
ATTACGTGAAGCGGGTATGGAAGTCACCGTAACCGAAGATACGATTACGTTAGACTCAAAGGGTCAGCGTCCGAAAGCGG
TAAACATTCGTACTATGCCGCATCCGGGGTTCCCAACCGATATGCAAGCGCAATTTACTTTGCTTAACGTTGTAGCGGAA
GGAACAAGCCGTATTACCGAAACGATTTTTGAAAATCGTTTTATGCATATTCCCGAACTAAATCGTATGGGGGCAAAAGG
TGAAATTGAGGGTAATACCGCAATTTGCCACGGTGTCGAAAAATTAAAATCGGCGGAAGTGATGGCGACGGATTTACGCG
CATCAATTAGCTTAGTATTAGCCGGTTGTATCGCTTCAGGTGAAACTATTGTGGATCGTATTTATCACATTGACCGCGGT
TACGAACATATTGAAGATAAATTACGTGGAATCGGGGCAAAAATCGAGAGATTTTCAACTAAGTTTGAAGAGTAA

Upstream 100 bases:

>100_bases
TAAGCGGTCGCTTTTGTAAAAAAATTTGCAAAAATGACCGCTTGTATTTTGAGTTATTCGGTTATCAATCGTCAATTATT
TATTTTCGAAGGATTTTATA

Downstream 100 bases:

>100_bases
TTTCCTACTGTTTTAAATAAAAATGACCGCATCAAGCGGTCATTTTTATAGGCAAAATTACATACCGGAATCTTTTTTCT
GTTTATGTGATTTTTTGCCG

Product: UDP-N-acetylglucosamine 1-carboxyvinyltransferase

Products: NA

Alternate protein names: Enoylpyruvate transferase; UDP-N-acetylglucosamine enolpyruvyl transferase; EPT

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MEKFRVHGPFTLSGTVDISGAKNAALPILFAAVLATEPVTLTNVPDLKDVDTTFKILRKLGVVVERDESGAVQIDASKID
HYVAPYELVKTMRASIWALAPLVARFHEGQVSLPGGCTIGARPVDMHISGLEKMGALIELDEGYVKATSNGRLHGARIYM
DKVSVGATLSVMMAATLAEGTTTIENAAREPEIVDTALFLNAMGAKISGAGTDTITIEGVERLTGCQHRIVADRIETGTF
LVAAAVSGGKITCRGTKADTLEAVIEKLREAGMEVTVTEDTITLDSKGQRPKAVNIRTMPHPGFPTDMQAQFTLLNVVAE
GTSRITETIFENRFMHIPELNRMGAKGEIEGNTAICHGVEKLKSAEVMATDLRASISLVLAGCIASGETIVDRIYHIDRG
YEHIEDKLRGIGAKIERFSTKFEE

Sequences:

>Translated_424_residues
MEKFRVHGPFTLSGTVDISGAKNAALPILFAAVLATEPVTLTNVPDLKDVDTTFKILRKLGVVVERDESGAVQIDASKID
HYVAPYELVKTMRASIWALAPLVARFHEGQVSLPGGCTIGARPVDMHISGLEKMGALIELDEGYVKATSNGRLHGARIYM
DKVSVGATLSVMMAATLAEGTTTIENAAREPEIVDTALFLNAMGAKISGAGTDTITIEGVERLTGCQHRIVADRIETGTF
LVAAAVSGGKITCRGTKADTLEAVIEKLREAGMEVTVTEDTITLDSKGQRPKAVNIRTMPHPGFPTDMQAQFTLLNVVAE
GTSRITETIFENRFMHIPELNRMGAKGEIEGNTAICHGVEKLKSAEVMATDLRASISLVLAGCIASGETIVDRIYHIDRG
YEHIEDKLRGIGAKIERFSTKFEE
>Mature_424_residues
MEKFRVHGPFTLSGTVDISGAKNAALPILFAAVLATEPVTLTNVPDLKDVDTTFKILRKLGVVVERDESGAVQIDASKID
HYVAPYELVKTMRASIWALAPLVARFHEGQVSLPGGCTIGARPVDMHISGLEKMGALIELDEGYVKATSNGRLHGARIYM
DKVSVGATLSVMMAATLAEGTTTIENAAREPEIVDTALFLNAMGAKISGAGTDTITIEGVERLTGCQHRIVADRIETGTF
LVAAAVSGGKITCRGTKADTLEAVIEKLREAGMEVTVTEDTITLDSKGQRPKAVNIRTMPHPGFPTDMQAQFTLLNVVAE
GTSRITETIFENRFMHIPELNRMGAKGEIEGNTAICHGVEKLKSAEVMATDLRASISLVLAGCIASGETIVDRIYHIDRG
YEHIEDKLRGIGAKIERFSTKFEE

Specific function: Cell wall formation. Adds enolpyruvyl to UDP-N- acetylglucosamine

COG id: COG0766

COG function: function code M; UDP-N-acetylglucosamine enolpyruvyl transferase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family. MurA subfamily

Homologues:

Organism=Escherichia coli, GI1789580, Length=417, Percent_Identity=73.1414868105516, Blast_Score=622, Evalue=1e-179,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURA_ACTP2 (A3N1T6)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053977.1
- ProteinModelPortal:   A3N1T6
- SMR:   A3N1T6
- STRING:   A3N1T6
- GeneID:   4850469
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1286
- eggNOG:   COG0766
- HOGENOM:   HBG482701
- OMA:   MVKTMRA
- PhylomeDB:   A3N1T6
- ProtClustDB:   PRK09369
- BioCyc:   APLE416269:APL_1286-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00111
- InterPro:   IPR001986
- InterPro:   IPR013792
- InterPro:   IPR005750
- Gene3D:   G3DSA:3.65.10.10
- PANTHER:   PTHR21090:SF4
- TIGRFAMs:   TIGR01072

Pfam domain/function: PF00275 EPSP_synthase; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =2.5.1.7

Molecular weight: Translated: 45648; Mature: 45648

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: ACT_SITE 117-117

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKFRVHGPFTLSGTVDISGAKNAALPILFAAVLATEPVTLTNVPDLKDVDTTFKILRKL
CCCEEECCCEEEEEEEEECCCCCCHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHH
GVVVERDESGAVQIDASKIDHYVAPYELVKTMRASIWALAPLVARFHEGQVSLPGGCTIG
CCEEEECCCCCEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEC
ARPVDMHISGLEKMGALIELDEGYVKATSNGRLHGARIYMDKVSVGATLSVMMAATLAEG
CCEEEHHHHHHHHCCCEEEECCCEEEECCCCCEEEEEEEEEECCCCHHHHHHHHHHHHCC
TTTIENAAREPEIVDTALFLNAMGAKISGAGTDTITIEGVERLTGCQHRIVADRIETGTF
CHHHHHHCCCCCHHHHHHHHHHCCCEEECCCCCEEEECCHHHHCCCHHHHHHHHHCCCEE
LVAAAVSGGKITCRGTKADTLEAVIEKLREAGMEVTVTEDTITLDSKGQRPKAVNIRTMP
EEEEEECCCEEEECCCCHHHHHHHHHHHHHCCCEEEEECCEEEECCCCCCCCEEEEEECC
HPGFPTDMQAQFTLLNVVAEGTSRITETIFENRFMHIPELNRMGAKGEIEGNTAICHGVE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCEECCCHHHHHHHH
KLKSAEVMATDLRASISLVLAGCIASGETIVDRIYHIDRGYEHIEDKLRGIGAKIERFST
HHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHH
KFEE
CCCC
>Mature Secondary Structure
MEKFRVHGPFTLSGTVDISGAKNAALPILFAAVLATEPVTLTNVPDLKDVDTTFKILRKL
CCCEEECCCEEEEEEEEECCCCCCHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHH
GVVVERDESGAVQIDASKIDHYVAPYELVKTMRASIWALAPLVARFHEGQVSLPGGCTIG
CCEEEECCCCCEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEC
ARPVDMHISGLEKMGALIELDEGYVKATSNGRLHGARIYMDKVSVGATLSVMMAATLAEG
CCEEEHHHHHHHHCCCEEEECCCEEEECCCCCEEEEEEEEEECCCCHHHHHHHHHHHHCC
TTTIENAAREPEIVDTALFLNAMGAKISGAGTDTITIEGVERLTGCQHRIVADRIETGTF
CHHHHHHCCCCCHHHHHHHHHHCCCEEECCCCCEEEECCHHHHCCCHHHHHHHHHCCCEE
LVAAAVSGGKITCRGTKADTLEAVIEKLREAGMEVTVTEDTITLDSKGQRPKAVNIRTMP
EEEEEECCCEEEECCCCHHHHHHHHHHHHHCCCEEEEECCEEEECCCCCCCCEEEEEECC
HPGFPTDMQAQFTLLNVVAEGTSRITETIFENRFMHIPELNRMGAKGEIEGNTAICHGVE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCEECCCHHHHHHHH
KLKSAEVMATDLRASISLVLAGCIASGETIVDRIYHIDRGYEHIEDKLRGIGAKIERFST
HHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHH
KFEE
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA