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The map label for this gene is sppA [H]

Identifier: 126208734

GI number: 126208734

Start: 1456773

End: 1458629

Strand: Direct

Name: sppA [H]

Synonym: APL_1268

Alternate gene names: 126208734

Gene position: 1456773-1458629 (Clockwise)

Preceding gene: 126208731

Following gene: 126208735

Centisome position: 64.05

GC content: 43.4

Gene sequence:

>1857_bases
ATGTTAAAAATATTAAAAGGGATTTATCAAATTTTCCGTTGTATTCGTGAATTTGTAATGAGCCTGTTTTTTATTATCTT
TGTATTGGTTTGCTTTGCTTTTCTGGCTTTAATCAATCAAGAAAGCGGCTCGACGAAGCATACGAATTTTATAGAAAAAG
GCGCATTAACGCTTAATTTAGACGGCTATTTAGCCGATAACCACGACAAATACGGCGATCTCCATCGTTTAGTACAGGCT
GAATTAGGCAATCACGAGCCGATAAAAATTTCCACTTTTGATGTGGTACGTGCGATTGCCAAAGCGGCTAAAGATGAACG
TATTACCGGATTGGTGCTGGATTTAGCCTATTTCCAAGGCGGCGACAGCGCCTCGTTACATTTTATCGGAAATGAAATTA
ACGAATTTAAAAAATCGGGTAAACCCGTCATTGCCGTCGGCGAACAATATAGCCAGCCGCAATACTATTTAGCCAGTTTC
GCCGATAAAATTTACCTGAATAAAGCCGGTTTTGTCGATATTCATGCGCTCAGTCACAGCAATGTTTATTTCAAAACGTT
GCTCGATAAAATTGAAGCCGAACCGCATATTTTCCGTGTCGGTACTTATAAGTCGGCGGTAGAGCCGTTTATCCGCGACG
ATATGTCCGCCGAAGCGAAGCAGAACGCCCAAACTTGGCTGACTTCCATTTGGCATAATATCCGCCAAGATATTGCGCAA
AATCGTAAGATTCCTGCCGAACAGGTGCTATTGGACAGCCAAACTTATATTGAAAAATACAGACAACTAAAAGGCGACGA
CGCGCAATATGCGTTAAGCCAACAATTAGTTACAGATATTGCAACAAACGTACAAATTCAGACCGCTTTAGTCGAACGAT
TCGGCAAAGATAAAGACGGACATTACAACAGTATCGATTATTTCGATTACGCAATGGAACTCAATGATCGTTTTAACGTA
CAAGCCGAACATAAAATTGCCGTGGTAAACGTCGAAGGGCAAATTATTTCGGGCGAAAGCGATGCTAGTTCCTCCGGCAG
CGATACGATCGTCAAATTATTACGTAAAGCCCGTGAAGACGATAACGTACGAGGCGTGATTTTAAGAGTAAACAGCCCGG
GCGGTAGCGCACTTGCTTCGGAAATTATCCGCCAAGAGGTGGAAGAAATTCAAAAAGCCGGTAAACCTGTCGTCGCATCA
ATGGGCGGTATGGCGGCTTCCGGCGGTTATTGGATTTCGGCGACCAGCGATAAAATTATCGCCAGTCCGACCACCATTAC
CGGTTCGATCGGGATTTTCGGCTTGGCAACCAGCTTTGAAAAAACCGCTAAAAATTTAGGCGTCACAGAAGACGGTATTT
CGCTTTCGCCGTTTGCTTCGTCCAGCCCGTTAAAAACGTTGCAGAAAGAACAAGCGGAAGTAATCCAAATCAGTATTGAA
AACGGCTACGACCGATTCCTAGAATTAGTCAGTCGCGGACGTAATATGTCTAAACAAGCGGTCGATAAAATCGCACAAGG
TCAAGTATGGTCGGGCGAAGACGCGTTAAAACACGGCTTAGTCGATGAATTAGGCGATTTTACCGCTGCTTACAGTGCGG
TTACCGAGTTAGTAAACCAACGACGTAAAGCGAATAACGAAGCGGAAATCGAACACTTTAGAAGCCAATGGATGATTGAT
AGCGACGATAGTCTGTTCGGTTCATTGCTAAAAGGCCCTAAACTCAAATTCCAGTTGAGTTCATGGTTAGGGCTTCCGGT
TGCAGCTCAAGCGCAGCAATCGCTCGAATTACTGCAAAAATTCAACGACCCTAAATATACTTATTTATATTGTCTTACCT
GCGGTACGATAAAATAG

Upstream 100 bases:

>100_bases
TCGTGTAAAAATTAGCATAAGAATAACATATTTTGTTAATTCCACGTCTTTTTTATGGTATTTTATTGCGCAAATTTCTT
ACGTTTTAATTAGGATAATT

Downstream 100 bases:

>100_bases
CATGTTACAAGCGGTCGGATTTGCAAATTTTTTCGCAAATCCGACCGCTTATTTTTTCTTCCCTCACCTCTCTTTTTTGT
TTATAGTAACGCCCTTTCCA

Product: protease 4

Products: NA

Alternate protein names: Endopeptidase IV; Protease IV; Signal peptide peptidase [H]

Number of amino acids: Translated: 618; Mature: 618

Protein sequence:

>618_residues
MLKILKGIYQIFRCIREFVMSLFFIIFVLVCFAFLALINQESGSTKHTNFIEKGALTLNLDGYLADNHDKYGDLHRLVQA
ELGNHEPIKISTFDVVRAIAKAAKDERITGLVLDLAYFQGGDSASLHFIGNEINEFKKSGKPVIAVGEQYSQPQYYLASF
ADKIYLNKAGFVDIHALSHSNVYFKTLLDKIEAEPHIFRVGTYKSAVEPFIRDDMSAEAKQNAQTWLTSIWHNIRQDIAQ
NRKIPAEQVLLDSQTYIEKYRQLKGDDAQYALSQQLVTDIATNVQIQTALVERFGKDKDGHYNSIDYFDYAMELNDRFNV
QAEHKIAVVNVEGQIISGESDASSSGSDTIVKLLRKAREDDNVRGVILRVNSPGGSALASEIIRQEVEEIQKAGKPVVAS
MGGMAASGGYWISATSDKIIASPTTITGSIGIFGLATSFEKTAKNLGVTEDGISLSPFASSSPLKTLQKEQAEVIQISIE
NGYDRFLELVSRGRNMSKQAVDKIAQGQVWSGEDALKHGLVDELGDFTAAYSAVTELVNQRRKANNEAEIEHFRSQWMID
SDDSLFGSLLKGPKLKFQLSSWLGLPVAAQAQQSLELLQKFNDPKYTYLYCLTCGTIK

Sequences:

>Translated_618_residues
MLKILKGIYQIFRCIREFVMSLFFIIFVLVCFAFLALINQESGSTKHTNFIEKGALTLNLDGYLADNHDKYGDLHRLVQA
ELGNHEPIKISTFDVVRAIAKAAKDERITGLVLDLAYFQGGDSASLHFIGNEINEFKKSGKPVIAVGEQYSQPQYYLASF
ADKIYLNKAGFVDIHALSHSNVYFKTLLDKIEAEPHIFRVGTYKSAVEPFIRDDMSAEAKQNAQTWLTSIWHNIRQDIAQ
NRKIPAEQVLLDSQTYIEKYRQLKGDDAQYALSQQLVTDIATNVQIQTALVERFGKDKDGHYNSIDYFDYAMELNDRFNV
QAEHKIAVVNVEGQIISGESDASSSGSDTIVKLLRKAREDDNVRGVILRVNSPGGSALASEIIRQEVEEIQKAGKPVVAS
MGGMAASGGYWISATSDKIIASPTTITGSIGIFGLATSFEKTAKNLGVTEDGISLSPFASSSPLKTLQKEQAEVIQISIE
NGYDRFLELVSRGRNMSKQAVDKIAQGQVWSGEDALKHGLVDELGDFTAAYSAVTELVNQRRKANNEAEIEHFRSQWMID
SDDSLFGSLLKGPKLKFQLSSWLGLPVAAQAQQSLELLQKFNDPKYTYLYCLTCGTIK
>Mature_618_residues
MLKILKGIYQIFRCIREFVMSLFFIIFVLVCFAFLALINQESGSTKHTNFIEKGALTLNLDGYLADNHDKYGDLHRLVQA
ELGNHEPIKISTFDVVRAIAKAAKDERITGLVLDLAYFQGGDSASLHFIGNEINEFKKSGKPVIAVGEQYSQPQYYLASF
ADKIYLNKAGFVDIHALSHSNVYFKTLLDKIEAEPHIFRVGTYKSAVEPFIRDDMSAEAKQNAQTWLTSIWHNIRQDIAQ
NRKIPAEQVLLDSQTYIEKYRQLKGDDAQYALSQQLVTDIATNVQIQTALVERFGKDKDGHYNSIDYFDYAMELNDRFNV
QAEHKIAVVNVEGQIISGESDASSSGSDTIVKLLRKAREDDNVRGVILRVNSPGGSALASEIIRQEVEEIQKAGKPVVAS
MGGMAASGGYWISATSDKIIASPTTITGSIGIFGLATSFEKTAKNLGVTEDGISLSPFASSSPLKTLQKEQAEVIQISIE
NGYDRFLELVSRGRNMSKQAVDKIAQGQVWSGEDALKHGLVDELGDFTAAYSAVTELVNQRRKANNEAEIEHFRSQWMID
SDDSLFGSLLKGPKLKFQLSSWLGLPVAAQAQQSLELLQKFNDPKYTYLYCLTCGTIK

Specific function: Digestion of the cleaved signal peptides. This activity is necessary to maintain proper secretion of mature proteins across the membrane [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=627, Percent_Identity=42.4242424242424, Blast_Score=487, Evalue=1e-139,
Organism=Escherichia coli, GI1787527, Length=160, Percent_Identity=26.25, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004634
- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 68656; Mature: 68656

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKILKGIYQIFRCIREFVMSLFFIIFVLVCFAFLALINQESGSTKHTNFIEKGALTLNL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCEEEEE
DGYLADNHDKYGDLHRLVQAELGNHEPIKISTFDVVRAIAKAAKDERITGLVLDLAYFQG
CCEEECCCCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHCCCCHHHHEEEHHHHCC
GDSASLHFIGNEINEFKKSGKPVIAVGEQYSQPQYYLASFADKIYLNKAGFVDIHALSHS
CCCCEEEEECHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHEECCCCEEEEEEECCC
NVYFKTLLDKIEAEPHIFRVGTYKSAVEPFIRDDMSAEAKQNAQTWLTSIWHNIRQDIAQ
CHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NRKIPAEQVLLDSQTYIEKYRQLKGDDAQYALSQQLVTDIATNVQIQTALVERFGKDKDG
CCCCCHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCC
HYNSIDYFDYAMELNDRFNVQAEHKIAVVNVEGQIISGESDASSSGSDTIVKLLRKARED
CCCCCHHHHHHEECCCCCCCEECCEEEEEEECCEEECCCCCCCCCCHHHHHHHHHHHCCC
DNVRGVILRVNSPGGSALASEIIRQEVEEIQKAGKPVVASMGGMAASGGYWISATSDKII
CCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCEECCCCEEEEECCCCEE
ASPTTITGSIGIFGLATSFEKTAKNLGVTEDGISLSPFASSSPLKTLQKEQAEVIQISIE
ECCCEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEEEC
NGYDRFLELVSRGRNMSKQAVDKIAQGQVWSGEDALKHGLVDELGDFTAAYSAVTELVNQ
CCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRKANNEAEIEHFRSQWMIDSDDSLFGSLLKGPKLKFQLSSWLGLPVAAQAQQSLELLQK
HHHCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHH
FNDPKYTYLYCLTCGTIK
CCCCCEEEEEEEECCCCC
>Mature Secondary Structure
MLKILKGIYQIFRCIREFVMSLFFIIFVLVCFAFLALINQESGSTKHTNFIEKGALTLNL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCEEEEE
DGYLADNHDKYGDLHRLVQAELGNHEPIKISTFDVVRAIAKAAKDERITGLVLDLAYFQG
CCEEECCCCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHCCCCHHHHEEEHHHHCC
GDSASLHFIGNEINEFKKSGKPVIAVGEQYSQPQYYLASFADKIYLNKAGFVDIHALSHS
CCCCEEEEECHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHEECCCCEEEEEEECCC
NVYFKTLLDKIEAEPHIFRVGTYKSAVEPFIRDDMSAEAKQNAQTWLTSIWHNIRQDIAQ
CHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NRKIPAEQVLLDSQTYIEKYRQLKGDDAQYALSQQLVTDIATNVQIQTALVERFGKDKDG
CCCCCHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCC
HYNSIDYFDYAMELNDRFNVQAEHKIAVVNVEGQIISGESDASSSGSDTIVKLLRKARED
CCCCCHHHHHHEECCCCCCCEECCEEEEEEECCEEECCCCCCCCCCHHHHHHHHHHHCCC
DNVRGVILRVNSPGGSALASEIIRQEVEEIQKAGKPVVASMGGMAASGGYWISATSDKII
CCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCEECCCCEEEEECCCCEE
ASPTTITGSIGIFGLATSFEKTAKNLGVTEDGISLSPFASSSPLKTLQKEQAEVIQISIE
ECCCEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEEEC
NGYDRFLELVSRGRNMSKQAVDKIAQGQVWSGEDALKHGLVDELGDFTAAYSAVTELVNQ
CCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRKANNEAEIEHFRSQWMIDSDDSLFGSLLKGPKLKFQLSSWLGLPVAAQAQQSLELLQK
HHHCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHH
FNDPKYTYLYCLTCGTIK
CCCCCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]