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The map label for this gene is malE [H]

Identifier: 126208703

GI number: 126208703

Start: 1421783

End: 1422967

Strand: Direct

Name: malE [H]

Synonym: APL_1237

Alternate gene names: 126208703

Gene position: 1421783-1422967 (Clockwise)

Preceding gene: 126208698

Following gene: 126208704

Centisome position: 62.51

GC content: 38.9

Gene sequence:

>1185_bases
ATGAAAAAACTTACTAAAACTGCATTAGCTGTATTAACAGGACTTTGTATTGCTCAAGGTGCAAATGCAAAAATTGTAGA
AGGTCAACTCAATATCTGGATTAGTGCTGATAAAGGCTATAACGGTTTAGCTGAAGTAGGTAAAAAATTTGAAGCAGAAA
CTGGGGTAAAAGTCATTGTAGAACATCCGGGAAAATTAGAAGAACTTTTCCCTCAAGTCGCCTCAACCGGTGATGGTCCT
GATGTGATTATCTTCGCCCATGACCGTTTCGGTGGCTACGCTCAAGCCGGTTTATTAGCCGAAGTAAAACCAAGTGCTGA
ATTTAAAGCAAAATTATCTGACATCGGTTGGGATGCGAATAAATATAACGGTAAACAAATTGCTTACCCAATTGCGATTG
AAGCGATTTCATTAATCTATAATAAAGATTTAGTTGCGACACCACCAAAAACATGGGAAGAAGTAGAGAAACTTGATAAA
GAATTAAAAGCTAAAGGTAAAAGTGCCATTATGTGGAACTTACAAGAACCCTATTTCACATGGCCGGTTATTTCGTCTCA
AGGCGCTTATGCATTCAAATTTACACCAAATGGTTATGACGTAAAAAATATTGGTGTCAACAATGAAGGTGCACAAAAAG
GCTTACAATTTGTTGTGGATTTAGTGAATAAAAAAGTGATTAATGCCGACATGGACTATGCAGTTTCAGAAGCGGCTTTC
AATAAAGGCAATACGGCTTTAACCATTAACGGACCATGGTCTTGGGCAAATATTGAGAAAAGTGGTATCAACTATGGTGT
CGCAGTACTTCCGACATTAAATGGTAAAGCATCAAAACCATTCGTTGGTGTATTAAGTGCAGCGGTAAATGCTTCTAGTC
CGAACCAAGACTTAGCCAAAGAGTTCTTAGAAAATCACTTATTAACCGATGAAGGACTCACAACCGTCAACAAAGACGCG
CCATTAGGTGCCGTTGCATTAAAATCTTTCCAAGAAAAACTTGCCAAAGATCCTCGTATCGCAGCAACTATGGCGAATGC
GGAAAACGGCGAAATTATGCCAAATATTCCACAAATGAGCCGTTTCTGGTATTCAGAAAAAGCAGCTATTGGCAATGCGA
CAAGCGGTCGTCAATCTGTAAAAGCAGCACTTGATGAAGCACAGGCCAAAATTGAAAAAGAATAA

Upstream 100 bases:

>100_bases
GGAGAACGGGGGATGATGAAATGATGAGTAAATTTTTTATTATGCTCTACGTAAAAGACTGGAAATCGGTCTATCTCCAA
AATCAAATGAGGAAAATCCT

Downstream 100 bases:

>100_bases
CTTTTGCTAACCTCACTGTTAGGACGCCTATTAGAGGCGTCCTATACCTCCAGCTTATCAATTTACAGGGGAAACACTAT
GCCAACATCAAATACAATGC

Product: maltose ABC transporter periplasmic protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: MMBP; Maltodextrin-binding protein [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MKKLTKTALAVLTGLCIAQGANAKIVEGQLNIWISADKGYNGLAEVGKKFEAETGVKVIVEHPGKLEELFPQVASTGDGP
DVIIFAHDRFGGYAQAGLLAEVKPSAEFKAKLSDIGWDANKYNGKQIAYPIAIEAISLIYNKDLVATPPKTWEEVEKLDK
ELKAKGKSAIMWNLQEPYFTWPVISSQGAYAFKFTPNGYDVKNIGVNNEGAQKGLQFVVDLVNKKVINADMDYAVSEAAF
NKGNTALTINGPWSWANIEKSGINYGVAVLPTLNGKASKPFVGVLSAAVNASSPNQDLAKEFLENHLLTDEGLTTVNKDA
PLGAVALKSFQEKLAKDPRIAATMANAENGEIMPNIPQMSRFWYSEKAAIGNATSGRQSVKAALDEAQAKIEKE

Sequences:

>Translated_394_residues
MKKLTKTALAVLTGLCIAQGANAKIVEGQLNIWISADKGYNGLAEVGKKFEAETGVKVIVEHPGKLEELFPQVASTGDGP
DVIIFAHDRFGGYAQAGLLAEVKPSAEFKAKLSDIGWDANKYNGKQIAYPIAIEAISLIYNKDLVATPPKTWEEVEKLDK
ELKAKGKSAIMWNLQEPYFTWPVISSQGAYAFKFTPNGYDVKNIGVNNEGAQKGLQFVVDLVNKKVINADMDYAVSEAAF
NKGNTALTINGPWSWANIEKSGINYGVAVLPTLNGKASKPFVGVLSAAVNASSPNQDLAKEFLENHLLTDEGLTTVNKDA
PLGAVALKSFQEKLAKDPRIAATMANAENGEIMPNIPQMSRFWYSEKAAIGNATSGRQSVKAALDEAQAKIEKE
>Mature_394_residues
MKKLTKTALAVLTGLCIAQGANAKIVEGQLNIWISADKGYNGLAEVGKKFEAETGVKVIVEHPGKLEELFPQVASTGDGP
DVIIFAHDRFGGYAQAGLLAEVKPSAEFKAKLSDIGWDANKYNGKQIAYPIAIEAISLIYNKDLVATPPKTWEEVEKLDK
ELKAKGKSAIMWNLQEPYFTWPVISSQGAYAFKFTPNGYDVKNIGVNNEGAQKGLQFVVDLVNKKVINADMDYAVSEAAF
NKGNTALTINGPWSWANIEKSGINYGVAVLPTLNGKASKPFVGVLSAAVNASSPNQDLAKEFLENHLLTDEGLTTVNKDA
PLGAVALKSFQEKLAKDPRIAATMANAENGEIMPNIPQMSRFWYSEKAAIGNATSGRQSVKAALDEAQAKIEKE

Specific function: Involved in the high-affinity maltose membrane transport system malEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides [H]

COG id: COG2182

COG function: function code G; Maltose-binding periplasmic proteins/domains

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790466, Length=386, Percent_Identity=68.3937823834197, Blast_Score=560, Evalue=1e-161,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006060
- InterPro:   IPR006059
- InterPro:   IPR006061 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 42479; Mature: 42479

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLTKTALAVLTGLCIAQGANAKIVEGQLNIWISADKGYNGLAEVGKKFEAETGVKVIV
CCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCCHHHHHHHHHCCCCCCEEEE
EHPGKLEELFPQVASTGDGPDVIIFAHDRFGGYAQAGLLAEVKPSAEFKAKLSDIGWDAN
ECCCHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHCCEEEECCCCHHHHHHHHHCCCCCC
KYNGKQIAYPIAIEAISLIYNKDLVATPPKTWEEVEKLDKELKAKGKSAIMWNLQEPYFT
CCCCCEEEEHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCEEE
WPVISSQGAYAFKFTPNGYDVKNIGVNNEGAQKGLQFVVDLVNKKVINADMDYAVSEAAF
CCEECCCCCEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHC
NKGNTALTINGPWSWANIEKSGINYGVAVLPTLNGKASKPFVGVLSAAVNASSPNQDLAK
CCCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHH
EFLENHLLTDEGLTTVNKDAPLGAVALKSFQEKLAKDPRIAATMANAENGEIMPNIPQMS
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHH
RFWYSEKAAIGNATSGRQSVKAALDEAQAKIEKE
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKLTKTALAVLTGLCIAQGANAKIVEGQLNIWISADKGYNGLAEVGKKFEAETGVKVIV
CCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCCHHHHHHHHHCCCCCCEEEE
EHPGKLEELFPQVASTGDGPDVIIFAHDRFGGYAQAGLLAEVKPSAEFKAKLSDIGWDAN
ECCCHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHCCEEEECCCCHHHHHHHHHCCCCCC
KYNGKQIAYPIAIEAISLIYNKDLVATPPKTWEEVEKLDKELKAKGKSAIMWNLQEPYFT
CCCCCEEEEHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCEEE
WPVISSQGAYAFKFTPNGYDVKNIGVNNEGAQKGLQFVVDLVNKKVINADMDYAVSEAAF
CCEECCCCCEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHC
NKGNTALTINGPWSWANIEKSGINYGVAVLPTLNGKASKPFVGVLSAAVNASSPNQDLAK
CCCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHH
EFLENHLLTDEGLTTVNKDAPLGAVALKSFQEKLAKDPRIAATMANAENGEIMPNIPQMS
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHH
RFWYSEKAAIGNATSGRQSVKAALDEAQAKIEKE
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]