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The map label for this gene is nfnB [C]

Identifier: 126208666

GI number: 126208666

Start: 1381046

End: 1381708

Strand: Direct

Name: nfnB [C]

Synonym: APL_1198

Alternate gene names: 126208666

Gene position: 1381046-1381708 (Clockwise)

Preceding gene: 126208660

Following gene: 126208667

Centisome position: 60.72

GC content: 41.63

Gene sequence:

>663_bases
ATGTTTAATATTGAAAAAGAAAACGTTTTAGCGGCATTTAAATATCGTCGTGCAACACGTAACTATGATGCGTCTAAAAA
AATCAGCGATGAAGATTTCGCTTATATTTTAGAGTTAGCACGTCTTTCACCAAGCTCGGTCGGTTCAGAGCCGTGGAAAT
TTGTGGTGATTCAAAACCAAGAATTACGTAATAAATTAAAACCGGTTGCGTGGGGTATGGCGGCACAAATGGACGCCGCA
AGCCATTTAGTGGTTTTACTTGCTAAGAAAAATGCAAGCCACGATAGCGAATACTTCAAAAAAGCACTTGAACAACGTGG
TTTAACTGCTGAGGAAATGGAAAAAACTTTAGCGTTATATAAACAATTCCACGTTGAAGATATTAAAATCGCCGGCAATG
AACGTGCGTTATTCGACTGGTGTTCAAAACAAACCTACATCGCATTAGGTAATATGATGAGCGGAGCAGCGATGATCGGT
ATCGATAGCTGCCCTATCGAGGGTTTTAACTACGATGCGGTGAATAAAATCCTTGCGGAAGCCGGCGCATTCGATCCGAA
TGAATACGGCGTTTCCGTAATGGCGACTTTCGGTTATCGTGCCGGTGAAATCGCACCGAAATCACGTAAACCGATCGAAG
AAATCGTAAGCTGGATTAAATAA

Upstream 100 bases:

>100_bases
TTAAAAGTTATATTTTACTCGGAAAGCAATTATGAAATCGCGTTGGGTTCTTGCACCTTTAGCTGAACTCACTAGGTTTA
TAAATAAAAAAGGAAAAACA

Downstream 100 bases:

>100_bases
TCCACACATAACGAAAACGGTATGAGATCCCTGATTTCATACCGTTTTTACTTATTTATTTCCCAACTCGAAAACCCTGC
CTTTCCAGCCTTGACGAAAT

Product: putative NAD(P)H nitroreductase

Products: NAD(P)(+); @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

Alternate protein names: NA

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MFNIEKENVLAAFKYRRATRNYDASKKISDEDFAYILELARLSPSSVGSEPWKFVVIQNQELRNKLKPVAWGMAAQMDAA
SHLVVLLAKKNASHDSEYFKKALEQRGLTAEEMEKTLALYKQFHVEDIKIAGNERALFDWCSKQTYIALGNMMSGAAMIG
IDSCPIEGFNYDAVNKILAEAGAFDPNEYGVSVMATFGYRAGEIAPKSRKPIEEIVSWIK

Sequences:

>Translated_220_residues
MFNIEKENVLAAFKYRRATRNYDASKKISDEDFAYILELARLSPSSVGSEPWKFVVIQNQELRNKLKPVAWGMAAQMDAA
SHLVVLLAKKNASHDSEYFKKALEQRGLTAEEMEKTLALYKQFHVEDIKIAGNERALFDWCSKQTYIALGNMMSGAAMIG
IDSCPIEGFNYDAVNKILAEAGAFDPNEYGVSVMATFGYRAGEIAPKSRKPIEEIVSWIK
>Mature_220_residues
MFNIEKENVLAAFKYRRATRNYDASKKISDEDFAYILELARLSPSSVGSEPWKFVVIQNQELRNKLKPVAWGMAAQMDAA
SHLVVLLAKKNASHDSEYFKKALEQRGLTAEEMEKTLALYKQFHVEDIKIAGNERALFDWCSKQTYIALGNMMSGAAMIG
IDSCPIEGFNYDAVNKILAEAGAFDPNEYGVSVMATFGYRAGEIAPKSRKPIEEIVSWIK

Specific function: Reduction Of A Variety Of Nitroaromatic Compounds Using NADH (And To Lesser Extent Nadph) As Source Of Reducing Equivalents; Two Electrons Are Transferred. Capable Of Reducing Nitrofurazone, Quinones And The Anti-Tumor Agent Cb1954 (5- (Aziridin-1-Yl)-2,4

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

Organism=Escherichia coli, GI1786792, Length=212, Percent_Identity=30.6603773584906, Blast_Score=84, Evalue=8e-18,

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 24704; Mature: 24704

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNIEKENVLAAFKYRRATRNYDASKKISDEDFAYILELARLSPSSVGSEPWKFVVIQNQ
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECH
ELRNKLKPVAWGMAAQMDAASHLVVLLAKKNASHDSEYFKKALEQRGLTAEEMEKTLALY
HHHHHHCHHHHHHHHHHHHHCEEEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
KQFHVEDIKIAGNERALFDWCSKQTYIALGNMMSGAAMIGIDSCPIEGFNYDAVNKILAE
HHHCCCEEEECCCCHHHHHHHCCCCEEEECCHHCCEEEEECCCCCCCCCCHHHHHHHHHH
AGAFDPNEYGVSVMATFGYRAGEIAPKSRKPIEEIVSWIK
HCCCCCCHHCEEEEEECCCCCCCCCCCCCCCHHHHHHHHC
>Mature Secondary Structure
MFNIEKENVLAAFKYRRATRNYDASKKISDEDFAYILELARLSPSSVGSEPWKFVVIQNQ
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECH
ELRNKLKPVAWGMAAQMDAASHLVVLLAKKNASHDSEYFKKALEQRGLTAEEMEKTLALY
HHHHHHCHHHHHHHHHHHHHCEEEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
KQFHVEDIKIAGNERALFDWCSKQTYIALGNMMSGAAMIGIDSCPIEGFNYDAVNKILAE
HHHCCCEEEECCCCHHHHHHHCCCCEEEECCHHCCEEEEECCCCCCCCCCHHHHHHHHHH
AGAFDPNEYGVSVMATFGYRAGEIAPKSRKPIEEIVSWIK
HCCCCCCHHCEEEEEECCCCCCCCCCCCCCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H; 6,7-Dihydropteridine [C]

Specific reaction: NAD(P)H + 6,7-Dihydropteridine = NAD(P)(+) + @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]