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The map label for this gene is yafC [C]

Identifier: 126208654

GI number: 126208654

Start: 1365058

End: 1365951

Strand: Reverse

Name: yafC [C]

Synonym: APL_1186

Alternate gene names: 126208654

Gene position: 1365951-1365058 (Counterclockwise)

Preceding gene: 126208656

Following gene: 126208648

Centisome position: 60.06

GC content: 45.64

Gene sequence:

>894_bases
ATGGATAGAATTACCGCAATTCAAGTGTTTTTAATGGTGGCAGAAACCGGCAGTTTTACCGCAACGGCGGAACGATTGGA
TCTTTCACGCCCGAAAGTTACCCGTGCAGTCGCCCTTATGGAAGAATGGTTTAATGCTCGGCTTTTGCAGCGCACTACCC
GCCATGTTTCACTGACCGATGCTGGCGAACAAGCGGTCGAATATTGCAAAAAAATGACGGAATTAACCTCGGCTATTGAA
CAGGATTTTTTAACTAAGCGAGGGGAATTGCGAGGCTCGTTGAAAATTGCCTCGAACGCAGCATTTGGTGGCACCCATTT
GCTCAATGCGATTCGCACTTTTTTAGCCAAACATCCGAAGCTGAATATCCAACTGATTTTGGCGGATAACACGGTAAATT
TAGTTGAAGAGGGAATCGATCTTGCTGTCCGTTTTACTAACAATCCCGATCCAAATGTTGTGGCTCGCCCATTATTCACT
TGCCATTCTTTATTAGTGGCAACGCCTGCCTATTTAGCTCAGCACGGCACAATTCACTCACCGCAAGAATTAGCTAATCA
TCGTTATTTAGCACACGCAAATGTAAACCGAAAAGAGTGGACATTTATCAAAGACGGGCAAGAAACGGTGTTGGAACTGA
CCAGCCAACTCACCATCAACGACACAGGGGCATTACTTAATTTCACTTTAGCAGACGGTGGCATTGCAATGTTGCCGAAG
TATTTTATTGAACAACAACTCAAAAACAATCAACTACAAATTGTGCTACCCGACTGGCAAATGCCGACTTATCAAGTGTA
CGCCGTCTATCCCTCCCGCCGCAAACTCCCTCAAACCGTCCGCAGCTTTGTGGATTTTTTAGTGGAAGAGTTTGAGGGGA
AGGGGGATTGGTAG

Upstream 100 bases:

>100_bases
TTTCAAATAACCTGTTGTCATTATATTGAAATAAAAAATGGGAAAAAGACTGGTTTCTGAATATAGTTGTTTTAAAAATT
GAAACAATAAAGGGTAAAAA

Downstream 100 bases:

>100_bases
GGGAAAACGATTGGAATGTCAGTGGAGTTTTTTTGGCTTGTATCGAGTGAGCGATGCAGAAACCGAGAAAGCGGTGCTAT
AAACCTTGCAAAGCAGGCAG

Product: putative transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MDRITAIQVFLMVAETGSFTATAERLDLSRPKVTRAVALMEEWFNARLLQRTTRHVSLTDAGEQAVEYCKKMTELTSAIE
QDFLTKRGELRGSLKIASNAAFGGTHLLNAIRTFLAKHPKLNIQLILADNTVNLVEEGIDLAVRFTNNPDPNVVARPLFT
CHSLLVATPAYLAQHGTIHSPQELANHRYLAHANVNRKEWTFIKDGQETVLELTSQLTINDTGALLNFTLADGGIAMLPK
YFIEQQLKNNQLQIVLPDWQMPTYQVYAVYPSRRKLPQTVRSFVDFLVEEFEGKGDW

Sequences:

>Translated_297_residues
MDRITAIQVFLMVAETGSFTATAERLDLSRPKVTRAVALMEEWFNARLLQRTTRHVSLTDAGEQAVEYCKKMTELTSAIE
QDFLTKRGELRGSLKIASNAAFGGTHLLNAIRTFLAKHPKLNIQLILADNTVNLVEEGIDLAVRFTNNPDPNVVARPLFT
CHSLLVATPAYLAQHGTIHSPQELANHRYLAHANVNRKEWTFIKDGQETVLELTSQLTINDTGALLNFTLADGGIAMLPK
YFIEQQLKNNQLQIVLPDWQMPTYQVYAVYPSRRKLPQTVRSFVDFLVEEFEGKGDW
>Mature_297_residues
MDRITAIQVFLMVAETGSFTATAERLDLSRPKVTRAVALMEEWFNARLLQRTTRHVSLTDAGEQAVEYCKKMTELTSAIE
QDFLTKRGELRGSLKIASNAAFGGTHLLNAIRTFLAKHPKLNIQLILADNTVNLVEEGIDLAVRFTNNPDPNVVARPLFT
CHSLLVATPAYLAQHGTIHSPQELANHRYLAHANVNRKEWTFIKDGQETVLELTSQLTINDTGALLNFTLADGGIAMLPK
YFIEQQLKNNQLQIVLPDWQMPTYQVYAVYPSRRKLPQTVRSFVDFLVEEFEGKGDW

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=288, Percent_Identity=30.2083333333333, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI1789639, Length=306, Percent_Identity=27.4509803921569, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI145693193, Length=304, Percent_Identity=31.25, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI87081978, Length=290, Percent_Identity=29.6551724137931, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1787128, Length=286, Percent_Identity=27.6223776223776, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI1787589, Length=293, Percent_Identity=28.6689419795222, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI1789440, Length=283, Percent_Identity=24.7349823321555, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1788706, Length=302, Percent_Identity=24.1721854304636, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787601, Length=146, Percent_Identity=27.3972602739726, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI157672245, Length=173, Percent_Identity=30.635838150289, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789173, Length=198, Percent_Identity=23.7373737373737, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788748, Length=167, Percent_Identity=28.1437125748503, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33498; Mature: 33498

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRITAIQVFLMVAETGSFTATAERLDLSRPKVTRAVALMEEWFNARLLQRTTRHVSLTD
CCCHHHHHHHHHHCCCCCCEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECC
AGEQAVEYCKKMTELTSAIEQDFLTKRGELRGSLKIASNAAFGGTHLLNAIRTFLAKHPK
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCC
LNIQLILADNTVNLVEEGIDLAVRFTNNPDPNVVARPLFTCHSLLVATPAYLAQHGTIHS
CEEEEEEECCHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCHHHHHHCCCCCC
PQELANHRYLAHANVNRKEWTFIKDGQETVLELTSQLTINDTGALLNFTLADGGIAMLPK
HHHHHCCEEEEECCCCCCCEEEEECCHHHHHHHHHCEEECCCCCEEEEEECCCCHHHHHH
YFIEQQLKNNQLQIVLPDWQMPTYQVYAVYPSRRKLPQTVRSFVDFLVEEFEGKGDW
HHHHHHHCCCCEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MDRITAIQVFLMVAETGSFTATAERLDLSRPKVTRAVALMEEWFNARLLQRTTRHVSLTD
CCCHHHHHHHHHHCCCCCCEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECC
AGEQAVEYCKKMTELTSAIEQDFLTKRGELRGSLKIASNAAFGGTHLLNAIRTFLAKHPK
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCC
LNIQLILADNTVNLVEEGIDLAVRFTNNPDPNVVARPLFTCHSLLVATPAYLAQHGTIHS
CEEEEEEECCHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCHHHHHHCCCCCC
PQELANHRYLAHANVNRKEWTFIKDGQETVLELTSQLTINDTGALLNFTLADGGIAMLPK
HHHHHCCEEEEECCCCCCCEEEEECCHHHHHHHHHCEEECCCCCEEEEEECCCCHHHHHH
YFIEQQLKNNQLQIVLPDWQMPTYQVYAVYPSRRKLPQTVRSFVDFLVEEFEGKGDW
HHHHHHHCCCCEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]