Click here to switch to the map view.

The map label for this gene is kefB [H]

Identifier: 126208649

GI number: 126208649

Start: 1358973

End: 1360778

Strand: Direct

Name: kefB [H]

Synonym: APL_1181

Alternate gene names: 126208649

Gene position: 1358973-1360778 (Clockwise)

Preceding gene: 126208646

Following gene: 126208650

Centisome position: 59.75

GC content: 45.07

Gene sequence:

>1806_bases
ATGGCGGCAGAAGGTGCAAGCCAACTTACCAGTGTGGTAGCTTTATTAGGGGCGGCAATTATTGCCGTACCGATTTTTAA
ACGTATCGGTTTAGGATCAGTGCTGGGCTATTTAGCCGGCGGTTTAGCCATCGGACCTTTTGGCTTCGGCCTGTTTACGG
ATTCGCACACTATTTTGCATATTGCCGAATTAGGTGTGGTGATGTTCCTGTTTTTAGTCGGATTGGAAATGCAACCGGCG
CATTTGTGGGGATTACGTAAGTATATCTTCGGCTTGGGAAGTTTTCAGGTAGTTGGTGCGTCGATTGCGCTTACCGGTTT
AGTGATGGCATTCGGCTATTCGTGGCAAGTGGCATTTGTGAGTGCAGCAGGTTTCGTATTAACTTCCACCGCAATTGTAA
TGCAGGTATTAAGCGAGCGAGGTGAAATGACTTCCGAGCGAGGTCGTAAAATGGTTTCCATCTTGCTATTCGAAGATATG
CTGATTGTACCGCTACTAGCCGTTGTGGCTTTTCTCTCGCCGATTCATTCAACAGAAGCCAGCACACCGATTTGGCAAAA
AATTGCGATTGCGGTTTTAGCGATGGTGTTCTTATTTATTATCGGCACCAAAATGCTAAATCCATTTTTTAAAATACTGG
CACGTACCAAAATCCGAGAAATGATGACTGCAGTCGCACTTTTCGTGGTGCTTGGTTCGGCATTATTAATGGAAGTGAGC
GGACTTTCCGCAGCAATGGGGGCATTCGCTGCCGGCGTATTGCTTTCGACCTCCAGCTTCCGTCATCAATTAGAAGTGGA
TATCGACCCGTTTAAAGGCTTATTGCTCGGCTTATTTTTCCTTGCGGTCGGGATGTCACTGGATTTGAATGTGGTATGGG
ATAACTTAGGGTTAATTCTATCCGGTGTGGCGTTGATGATGTTGATTAAAGGCGGGGTAATTTTCCTAGTGGCTCGTCTT
TCCGGCAGTAACAAATTAGATGCGCACGACCGTGCGATTGTGATGGCGCAGGGTGGTGAATTTGCGTTTGTATTATTTGC
TGCAGCGCACGCTCAAAAAGTAATTGATGACACAGTACACGCCAATATGACGGCAATTGTGGTGCTATCGATGGTCTTCA
CGCCGTTAATGATTATTGCGAGCCAGAAATTTATCGTGCCTCGTTTGCAAAAAATTGACGAAATCAAACCGCATGATCAT
ATTGAAGAACAGAACCCGATTATCTTGGTCGGCTTAGGTCGTTTCGGGCAAGTGGTCAATCATTTATTGCAAATGACAGG
CTATCATCCGACCATTATTGAACTGAATCCAAAACTTATCGCTGCAATGAAAAAACGTGGCGTAAAAAGTTATTACGGTA
ACGGTGCTCACCCTGATTTACTTAAAGCGGCAGGAATTCAAACCGCACAAATGCTGATTGTGGTGATTGATAATCCGAAA
CAAACGTTAGAAATCGTTAAATATGCACGCAGCGTAAATCCGCATATTAAAATTATCGCCCGTGCTTACGACCGTTATCA
CGTTTACGACTTGGTGCAATCCGGTGCGGATATTGAAGTACGCGAAACCTTTGACAGCGCATTGCGTACCGGCAAACAAG
CGTTACGAGAACTTGGGCTGGACGCGGACAAAGTGCATGAAATCGGCAATATGTATTTCGGCAAAGATCGCCATAGCATT
AAGCTTATGTCGGAAGTGTACGACCCGAAAATCGAGCGTTTTAAAAACGAAGAAATGTATCGTACCGCTCTCGAACAAGA
CCAAGAAATTATGGCGGAAATCCAAAAGATTTTGGCGAGAGAGTAA

Upstream 100 bases:

>100_bases
CGCTTGCCTGATCTTTTTCTAACTTTTCAATTCTTATCAAAGCTTTAGCTATTTTATGCTATTTTTATAATAAGATTTTT
ATTTTGAATTCGGAGGTTTT

Downstream 100 bases:

>100_bases
TATTTACAAGCGGTTAAATTTTGCAAAAAGTTGGCGAATTTTAACCGCTTTTTATTCCCTATTACTTATAATCCAATATA
CTTATCTCTTCTATTTATAA

Product: glutathione-regulated potassium-efflux system protein

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 601; Mature: 600

Protein sequence:

>601_residues
MAAEGASQLTSVVALLGAAIIAVPIFKRIGLGSVLGYLAGGLAIGPFGFGLFTDSHTILHIAELGVVMFLFLVGLEMQPA
HLWGLRKYIFGLGSFQVVGASIALTGLVMAFGYSWQVAFVSAAGFVLTSTAIVMQVLSERGEMTSERGRKMVSILLFEDM
LIVPLLAVVAFLSPIHSTEASTPIWQKIAIAVLAMVFLFIIGTKMLNPFFKILARTKIREMMTAVALFVVLGSALLMEVS
GLSAAMGAFAAGVLLSTSSFRHQLEVDIDPFKGLLLGLFFLAVGMSLDLNVVWDNLGLILSGVALMMLIKGGVIFLVARL
SGSNKLDAHDRAIVMAQGGEFAFVLFAAAHAQKVIDDTVHANMTAIVVLSMVFTPLMIIASQKFIVPRLQKIDEIKPHDH
IEEQNPIILVGLGRFGQVVNHLLQMTGYHPTIIELNPKLIAAMKKRGVKSYYGNGAHPDLLKAAGIQTAQMLIVVIDNPK
QTLEIVKYARSVNPHIKIIARAYDRYHVYDLVQSGADIEVRETFDSALRTGKQALRELGLDADKVHEIGNMYFGKDRHSI
KLMSEVYDPKIERFKNEEMYRTALEQDQEIMAEIQKILARE

Sequences:

>Translated_601_residues
MAAEGASQLTSVVALLGAAIIAVPIFKRIGLGSVLGYLAGGLAIGPFGFGLFTDSHTILHIAELGVVMFLFLVGLEMQPA
HLWGLRKYIFGLGSFQVVGASIALTGLVMAFGYSWQVAFVSAAGFVLTSTAIVMQVLSERGEMTSERGRKMVSILLFEDM
LIVPLLAVVAFLSPIHSTEASTPIWQKIAIAVLAMVFLFIIGTKMLNPFFKILARTKIREMMTAVALFVVLGSALLMEVS
GLSAAMGAFAAGVLLSTSSFRHQLEVDIDPFKGLLLGLFFLAVGMSLDLNVVWDNLGLILSGVALMMLIKGGVIFLVARL
SGSNKLDAHDRAIVMAQGGEFAFVLFAAAHAQKVIDDTVHANMTAIVVLSMVFTPLMIIASQKFIVPRLQKIDEIKPHDH
IEEQNPIILVGLGRFGQVVNHLLQMTGYHPTIIELNPKLIAAMKKRGVKSYYGNGAHPDLLKAAGIQTAQMLIVVIDNPK
QTLEIVKYARSVNPHIKIIARAYDRYHVYDLVQSGADIEVRETFDSALRTGKQALRELGLDADKVHEIGNMYFGKDRHSI
KLMSEVYDPKIERFKNEEMYRTALEQDQEIMAEIQKILARE
>Mature_600_residues
AAEGASQLTSVVALLGAAIIAVPIFKRIGLGSVLGYLAGGLAIGPFGFGLFTDSHTILHIAELGVVMFLFLVGLEMQPAH
LWGLRKYIFGLGSFQVVGASIALTGLVMAFGYSWQVAFVSAAGFVLTSTAIVMQVLSERGEMTSERGRKMVSILLFEDML
IVPLLAVVAFLSPIHSTEASTPIWQKIAIAVLAMVFLFIIGTKMLNPFFKILARTKIREMMTAVALFVVLGSALLMEVSG
LSAAMGAFAAGVLLSTSSFRHQLEVDIDPFKGLLLGLFFLAVGMSLDLNVVWDNLGLILSGVALMMLIKGGVIFLVARLS
GSNKLDAHDRAIVMAQGGEFAFVLFAAAHAQKVIDDTVHANMTAIVVLSMVFTPLMIIASQKFIVPRLQKIDEIKPHDHI
EEQNPIILVGLGRFGQVVNHLLQMTGYHPTIIELNPKLIAAMKKRGVKSYYGNGAHPDLLKAAGIQTAQMLIVVIDNPKQ
TLEIVKYARSVNPHIKIIARAYDRYHVYDLVQSGADIEVRETFDSALRTGKQALRELGLDADKVHEIGNMYFGKDRHSIK
LMSEVYDPKIERFKNEEMYRTALEQDQEIMAEIQKILARE

Specific function: Transport system that facilitate potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=402, Percent_Identity=26.3681592039801, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1786232, Length=593, Percent_Identity=38.9544688026981, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1789749, Length=598, Percent_Identity=38.2943143812709, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1786685, Length=526, Percent_Identity=28.1368821292776, Blast_Score=160, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 65695; Mature: 65564

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAEGASQLTSVVALLGAAIIAVPIFKRIGLGSVLGYLAGGLAIGPFGFGLFTDSHTILH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEECCCCCHHCCCCCHHHH
IAELGVVMFLFLVGLEMQPAHLWGLRKYIFGLGSFQVVGASIALTGLVMAFGYSWQVAFV
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEHHH
SAAGFVLTSTAIVMQVLSERGEMTSERGRKMVSILLFEDMLIVPLLAVVAFLSPIHSTEA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
STPIWQKIAIAVLAMVFLFIIGTKMLNPFFKILARTKIREMMTAVALFVVLGSALLMEVS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLSAAMGAFAAGVLLSTSSFRHQLEVDIDPFKGLLLGLFFLAVGMSLDLNVVWDNLGLIL
HHHHHHHHHHHHHHHHCHHHCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHH
SGVALMMLIKGGVIFLVARLSGSNKLDAHDRAIVMAQGGEFAFVLFAAAHAQKVIDDTVH
HHHHHHHHHHCCHHEEEEECCCCCCCCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHH
ANMTAIVVLSMVFTPLMIIASQKFIVPRLQKIDEIKPHDHIEEQNPIILVGLGRFGQVVN
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHH
HLLQMTGYHPTIIELNPKLIAAMKKRGVKSYYGNGAHPDLLKAAGIQTAQMLIVVIDNPK
HHHHHCCCCCEEEEECHHHHHHHHHCCCHHHCCCCCCHHHHHHCCCCEEEEEEEEECCCH
QTLEIVKYARSVNPHIKIIARAYDRYHVYDLVQSGADIEVRETFDSALRTGKQALRELGL
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHCC
DADKVHEIGNMYFGKDRHSIKLMSEVYDPKIERFKNEEMYRTALEQDQEIMAEIQKILAR
CHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC
E
C
>Mature Secondary Structure 
AAEGASQLTSVVALLGAAIIAVPIFKRIGLGSVLGYLAGGLAIGPFGFGLFTDSHTILH
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEECCCCCHHCCCCCHHHH
IAELGVVMFLFLVGLEMQPAHLWGLRKYIFGLGSFQVVGASIALTGLVMAFGYSWQVAFV
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEHHH
SAAGFVLTSTAIVMQVLSERGEMTSERGRKMVSILLFEDMLIVPLLAVVAFLSPIHSTEA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
STPIWQKIAIAVLAMVFLFIIGTKMLNPFFKILARTKIREMMTAVALFVVLGSALLMEVS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLSAAMGAFAAGVLLSTSSFRHQLEVDIDPFKGLLLGLFFLAVGMSLDLNVVWDNLGLIL
HHHHHHHHHHHHHHHHCHHHCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHH
SGVALMMLIKGGVIFLVARLSGSNKLDAHDRAIVMAQGGEFAFVLFAAAHAQKVIDDTVH
HHHHHHHHHHCCHHEEEEECCCCCCCCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHH
ANMTAIVVLSMVFTPLMIIASQKFIVPRLQKIDEIKPHDHIEEQNPIILVGLGRFGQVVN
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHH
HLLQMTGYHPTIIELNPKLIAAMKKRGVKSYYGNGAHPDLLKAAGIQTAQMLIVVIDNPK
HHHHHCCCCCEEEEECHHHHHHHHHCCCHHHCCCCCCHHHHHHCCCCEEEEEEEEECCCH
QTLEIVKYARSVNPHIKIIARAYDRYHVYDLVQSGADIEVRETFDSALRTGKQALRELGL
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHCC
DADKVHEIGNMYFGKDRHSIKLMSEVYDPKIERFKNEEMYRTALEQDQEIMAEIQKILAR
CHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]