Click here to switch to the map view.

The map label for this gene is ispG

Identifier: 126208644

GI number: 126208644

Start: 1351788

End: 1352900

Strand: Reverse

Name: ispG

Synonym: APL_1176

Alternate gene names: 126208644

Gene position: 1352900-1351788 (Counterclockwise)

Preceding gene: 126208645

Following gene: 126208643

Centisome position: 59.48

GC content: 45.28

Gene sequence:

>1113_bases
ATGTCAATTCATCAATCGCCAATTAAACGTCGCGAATCTAAAAAGATTTGGGTGGGGAATGTCGCAGTCGGCGGCGATGC
GCCGATTTCGGTGCAGTCGATGACCAATACGCGTACCACCGATGTGGAAGCTACCGTTGCACAAATCAAATCGCTTGAGC
GTGTCGGTGCGGATATCGTACGCGTTTCGGTACCGACTATGGATGCGGCGGAAGCGTTTAAAGTGATTAAACAGCAAGTG
AACGTACCGCTTGTGGCGGATATTCATTTCGATTATCGTATCGCATTAAAAGTGGCGGAATACGGTGTGGATTGTTTACG
TATCAATCCGGGTAATATCGGTAACGAAGAGCGTATTCGCGCCGTTGTGGACTGTGCGAAAGATAAAAATATTCCGATTC
GAATCGGTGTGAATGCCGGTTCACTTGAACGTGATTTACAAGAGAAATACGGCGAGCCGACTCCGCAGGCATTATTGGAA
TCGGCATTACGTCATGTAGATATTTTAGATCGCTTTAATTTTGAGAACTTTAAAGTGAGCGTAAAGGCATCCGATGTTTT
TCTAGCGGTCGAATCTTATCGTCTTTTAGCCAAACAAATCGTACAGCCGTTGCATTTAGGCATTACCGAAGCGGGCGGTG
CGAGAGCCGGTTCGGTCAAATCCGCAGTCGGTTTAGGACTATTACTGTCGGAAGGTATCGGTGATACGTTGCGTATTTCG
CTTGCAGCGGATCCGGTTGAAGAAGTGAAAGTCGGTTTCGATATTTTAAAATCGTTGCGTATTCGTTCTCGCGGTATCAA
TTTCATCGCTTGCCCGACTTGTTCACGTCAAGAAATCGACGTTATTGCTACGGTAAATGCGTTGGAACAACGTTTGGAAG
ATATTTTAACTCCGATGGACGTTTCCATTATCGGTTGTGTGGTAAACGGGCCGGGTGAAGCGCTGATTTCCGATTTAGGG
GTAACCGGTTCCAATAAAATGAGCGGTTTCTATCTTGACGGCGTTCGCCAAAAAGAGCGTTTCGATAATGAAAAATTAAT
TGATCAACTGGAAGCAAAAATTCGAGCAAGAGTTGCCGAACAACACAATCGAATTCAAATCGAACAAATTTAA

Upstream 100 bases:

>100_bases
GGCGAATGTAAAACTTTATTATAAAGGCCAAGAAGTACCGTTAAAAATTGACGGGCGTGTTGCTCGTATTAGATTACCTT
TAGCACAATAAGGATTGGAA

Downstream 100 bases:

>100_bases
TTTTAGTAACCACGGTGACACCGATTTACGGATATTGTGCAAAACAAGCGGTCAATTTTCTTCAAAAATTTACCAGCCGT
ACTATTTTGTGTTGTCCGTG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MSIHQSPIKRRESKKIWVGNVAVGGDAPISVQSMTNTRTTDVEATVAQIKSLERVGADIVRVSVPTMDAAEAFKVIKQQV
NVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEERIRAVVDCAKDKNIPIRIGVNAGSLERDLQEKYGEPTPQALLE
SALRHVDILDRFNFENFKVSVKASDVFLAVESYRLLAKQIVQPLHLGITEAGGARAGSVKSAVGLGLLLSEGIGDTLRIS
LAADPVEEVKVGFDILKSLRIRSRGINFIACPTCSRQEIDVIATVNALEQRLEDILTPMDVSIIGCVVNGPGEALISDLG
VTGSNKMSGFYLDGVRQKERFDNEKLIDQLEAKIRARVAEQHNRIQIEQI

Sequences:

>Translated_370_residues
MSIHQSPIKRRESKKIWVGNVAVGGDAPISVQSMTNTRTTDVEATVAQIKSLERVGADIVRVSVPTMDAAEAFKVIKQQV
NVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEERIRAVVDCAKDKNIPIRIGVNAGSLERDLQEKYGEPTPQALLE
SALRHVDILDRFNFENFKVSVKASDVFLAVESYRLLAKQIVQPLHLGITEAGGARAGSVKSAVGLGLLLSEGIGDTLRIS
LAADPVEEVKVGFDILKSLRIRSRGINFIACPTCSRQEIDVIATVNALEQRLEDILTPMDVSIIGCVVNGPGEALISDLG
VTGSNKMSGFYLDGVRQKERFDNEKLIDQLEAKIRARVAEQHNRIQIEQI
>Mature_369_residues
SIHQSPIKRRESKKIWVGNVAVGGDAPISVQSMTNTRTTDVEATVAQIKSLERVGADIVRVSVPTMDAAEAFKVIKQQVN
VPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEERIRAVVDCAKDKNIPIRIGVNAGSLERDLQEKYGEPTPQALLES
ALRHVDILDRFNFENFKVSVKASDVFLAVESYRLLAKQIVQPLHLGITEAGGARAGSVKSAVGLGLLLSEGIGDTLRISL
AADPVEEVKVGFDILKSLRIRSRGINFIACPTCSRQEIDVIATVNALEQRLEDILTPMDVSIIGCVVNGPGEALISDLGV
TGSNKMSGFYLDGVRQKERFDNEKLIDQLEAKIRARVAEQHNRIQIEQI

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=369, Percent_Identity=84.2818428184282, Blast_Score=635, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_ACTP2 (A3N1H8)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053869.1
- ProteinModelPortal:   A3N1H8
- STRING:   A3N1H8
- GeneID:   4848995
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1176
- eggNOG:   COG0821
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- PhylomeDB:   A3N1H8
- ProtClustDB:   PRK00366
- BioCyc:   APLE416269:APL_1176-MONOMER
- HAMAP:   MF_00159
- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE; SSF51717 DHP_synth_like

EC number: =1.17.7.1

Molecular weight: Translated: 40484; Mature: 40353

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIHQSPIKRRESKKIWVGNVAVGGDAPISVQSMTNTRTTDVEATVAQIKSLERVGADIV
CCCCCCHHHHCCCCEEEEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEE
RVSVPTMDAAEAFKVIKQQVNVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEERIR
EEECCCCCHHHHHHHHHHHCCCCEEEEEECCEEEEEEEHHCCCEEEEECCCCCCCHHHHH
AVVDCAKDKNIPIRIGVNAGSLERDLQEKYGEPTPQALLESALRHVDILDRFNFENFKVS
HHHHHHCCCCCCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEE
VKASDVFLAVESYRLLAKQIVQPLHLGITEAGGARAGSVKSAVGLGLLLSEGIGDTLRIS
EEECEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEE
LAADPVEEVKVGFDILKSLRIRSRGINFIACPTCSRQEIDVIATVNALEQRLEDILTPMD
EECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHEEHHHHHHHHHHHHCCCCC
VSIIGCVVNGPGEALISDLGVTGSNKMSGFYLDGVRQKERFDNEKLIDQLEAKIRARVAE
EEEEEEEECCCHHHHHHHCCCCCCCCCCCEEECCCHHHHHCCHHHHHHHHHHHHHHHHHH
QHNRIQIEQI
HCCCEEEEEC
>Mature Secondary Structure 
SIHQSPIKRRESKKIWVGNVAVGGDAPISVQSMTNTRTTDVEATVAQIKSLERVGADIV
CCCCCHHHHCCCCEEEEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEE
RVSVPTMDAAEAFKVIKQQVNVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEERIR
EEECCCCCHHHHHHHHHHHCCCCEEEEEECCEEEEEEEHHCCCEEEEECCCCCCCHHHHH
AVVDCAKDKNIPIRIGVNAGSLERDLQEKYGEPTPQALLESALRHVDILDRFNFENFKVS
HHHHHHCCCCCCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEE
VKASDVFLAVESYRLLAKQIVQPLHLGITEAGGARAGSVKSAVGLGLLLSEGIGDTLRIS
EEECEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEE
LAADPVEEVKVGFDILKSLRIRSRGINFIACPTCSRQEIDVIATVNALEQRLEDILTPMD
EECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHEEHHHHHHHHHHHHCCCCC
VSIIGCVVNGPGEALISDLGVTGSNKMSGFYLDGVRQKERFDNEKLIDQLEAKIRARVAE
EEEEEEEECCCHHHHHHHCCCCCCCCCCCEEECCCHHHHHCCHHHHHHHHHHHHHHHHHH
QHNRIQIEQI
HCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA