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The map label for this gene is hisS [H]

Identifier: 126208643

GI number: 126208643

Start: 1350389

End: 1351630

Strand: Reverse

Name: hisS [H]

Synonym: APL_1175

Alternate gene names: 126208643

Gene position: 1351630-1350389 (Counterclockwise)

Preceding gene: 126208644

Following gene: 126208642

Centisome position: 59.43

GC content: 45.09

Gene sequence:

>1242_bases
ATGAACGATTGCTCACCGACAGAATCGCCGTTATGGCAGTGGATTGAAAATAAAGTACGTAACGTATTAGCCGGTTACGG
TTATTCCGAAGTGCGTATGCCGATTGTCGAAAGCACCCCGCTTTTCGCACGTGCAATCGGCGAAGTGACCGATGTGGTTT
CAAAAGAAATGTACACCTTCTGGGATAATGACGAACAATTAACCCTTCGTCCGGAAGGGACGGCAGGTTGCGTGCGTGCG
GCGATCGAACGCGGGTGGATTTACAATAACGAACAACGTTTATGGTATATGGGTCCAATGTTCCGTCACGAGCGTCCGCA
AAAAGGTCGTTACCGCCAATTTCACCAAGCCGGTGTAGAGGTTTTCGGTATTCCTAATCCGGAAATTGATGCGGAATTAA
TTATTTTAACCGCACGTTTATGGAAAGAACTGGGCATCGATCAACACGTGTCTTTACAATTAAACTCTATCGGTTCGCTT
GAAGCTCGTGCGAATTATCGTTCGGCATTGGTAAAATTCTTAGAAAATCACACTGCTTTAATGAGCGATGAAGAAAAAGA
ACGTTTAGTTAAAAATCCGTTACGTATTCTTGATACCAAAAACCAGGCGTTACAAGAAGTACTTAATGATGCGCCGAAAT
TATTGGATTACTTAGATGATGACAGCCGTGAGCATTTTGCGCACCTTTGTCATTTACTAGATGCGATGGGTATCACTTAT
GAAGTGAATCCGAAACTGGTACGCGGTTTGGATTACTACAATAAAACCGTATTCGAGTGGGTAACTTCGGCATTAGGTTC
TCAAGGCACGGTATGCGGCGGCGGCCGTTATGACGGCTTGGTCGAACAACTCGGCGGTCATGCAACGCAAGGCGTCGGTT
TTGCGATGGGCTTAGAGCGTTTGGTTCTGCTTGTTCAAGAAGTGAATAAAGAAATCAACTTACCGAAAGCGGTTGATGTC
TATGTGGTTTATTCGGGTGAAGGCGCGACTTTAAATGCGTTCCAAATGGCGGAACGTATCCGTAGCGAATTACCGCAATT
AGGTGTGATGACTCACTGCTCGGGCGGTAACTTCAAAAAACAATTTAAACGTGCGGATAAAGTGGAAGCGCAAATCGCAT
TGGTTATCGGCGAAAGCGAAGTTGAACAACAAACCGTGGTGCTAAAAGATTTACAAAGCGGTGCGGAACAAGTCATAATC
GCGCAAGCGGATTTAATCGCGGAACTTACAAAACGTTTTTAA

Upstream 100 bases:

>100_bases
TTCTTCAAAAATTTACCAGCCGTACTATTTTGTGTTGTCCGTGGTTTTAGCGTAATAAAAGGAAAATAAAGTGGCTAAAA
ATATTCAAGCAATTCGTGGG

Downstream 100 bases:

>100_bases
CATTAGGAAAAATGAATGAGTAACGAATATTTAAATAAAACGGAAGAACAACAGTTCGAAGAGGCGAAGAATTGGTTTAA
AGAGAACGGTACGCCGATTT

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MNDCSPTESPLWQWIENKVRNVLAGYGYSEVRMPIVESTPLFARAIGEVTDVVSKEMYTFWDNDEQLTLRPEGTAGCVRA
AIERGWIYNNEQRLWYMGPMFRHERPQKGRYRQFHQAGVEVFGIPNPEIDAELIILTARLWKELGIDQHVSLQLNSIGSL
EARANYRSALVKFLENHTALMSDEEKERLVKNPLRILDTKNQALQEVLNDAPKLLDYLDDDSREHFAHLCHLLDAMGITY
EVNPKLVRGLDYYNKTVFEWVTSALGSQGTVCGGGRYDGLVEQLGGHATQGVGFAMGLERLVLLVQEVNKEINLPKAVDV
YVVYSGEGATLNAFQMAERIRSELPQLGVMTHCSGGNFKKQFKRADKVEAQIALVIGESEVEQQTVVLKDLQSGAEQVII
AQADLIAELTKRF

Sequences:

>Translated_413_residues
MNDCSPTESPLWQWIENKVRNVLAGYGYSEVRMPIVESTPLFARAIGEVTDVVSKEMYTFWDNDEQLTLRPEGTAGCVRA
AIERGWIYNNEQRLWYMGPMFRHERPQKGRYRQFHQAGVEVFGIPNPEIDAELIILTARLWKELGIDQHVSLQLNSIGSL
EARANYRSALVKFLENHTALMSDEEKERLVKNPLRILDTKNQALQEVLNDAPKLLDYLDDDSREHFAHLCHLLDAMGITY
EVNPKLVRGLDYYNKTVFEWVTSALGSQGTVCGGGRYDGLVEQLGGHATQGVGFAMGLERLVLLVQEVNKEINLPKAVDV
YVVYSGEGATLNAFQMAERIRSELPQLGVMTHCSGGNFKKQFKRADKVEAQIALVIGESEVEQQTVVLKDLQSGAEQVII
AQADLIAELTKRF
>Mature_413_residues
MNDCSPTESPLWQWIENKVRNVLAGYGYSEVRMPIVESTPLFARAIGEVTDVVSKEMYTFWDNDEQLTLRPEGTAGCVRA
AIERGWIYNNEQRLWYMGPMFRHERPQKGRYRQFHQAGVEVFGIPNPEIDAELIILTARLWKELGIDQHVSLQLNSIGSL
EARANYRSALVKFLENHTALMSDEEKERLVKNPLRILDTKNQALQEVLNDAPKLLDYLDDDSREHFAHLCHLLDAMGITY
EVNPKLVRGLDYYNKTVFEWVTSALGSQGTVCGGGRYDGLVEQLGGHATQGVGFAMGLERLVLLVQEVNKEINLPKAVDV
YVVYSGEGATLNAFQMAERIRSELPQLGVMTHCSGGNFKKQFKRADKVEAQIALVIGESEVEQQTVVLKDLQSGAEQVII
AQADLIAELTKRF

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788861, Length=410, Percent_Identity=66.8292682926829, Blast_Score=561, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI71993686, Length=453, Percent_Identity=24.7240618101545, Blast_Score=72, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI71993693, Length=453, Percent_Identity=24.7240618101545, Blast_Score=71, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6325290, Length=454, Percent_Identity=23.3480176211454, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 46561; Mature: 46561

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDCSPTESPLWQWIENKVRNVLAGYGYSEVRMPIVESTPLFARAIGEVTDVVSKEMYTF
CCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEE
WDNDEQLTLRPEGTAGCVRAAIERGWIYNNEQRLWYMGPMFRHERPQKGRYRQFHQAGVE
ECCCCEEEECCCCCHHHHHHHHHHCCEECCCCEEEEECCHHHCCCCCCCHHHHHHHCCCE
VFGIPNPEIDAELIILTARLWKELGIDQHVSLQLNSIGSLEARANYRSALVKFLENHTAL
EEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
MSDEEKERLVKNPLRILDTKNQALQEVLNDAPKLLDYLDDDSREHFAHLCHLLDAMGITY
CCCHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEE
EVNPKLVRGLDYYNKTVFEWVTSALGSQGTVCGGGRYDGLVEQLGGHATQGVGFAMGLER
ECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHH
LVLLVQEVNKEINLPKAVDVYVVYSGEGATLNAFQMAERIRSELPQLGVMTHCSGGNFKK
HHHHHHHHHHHCCCCCEEEEEEEECCCCCEEHHHHHHHHHHHHCCCCCEEEECCCCCHHH
QFKRADKVEAQIALVIGESEVEQQTVVLKDLQSGAEQVIIAQADLIAELTKRF
HHHHHHHHCEEEEEEECCCHHHHHHHHHHHHHCCHHEEEEHHHHHHHHHHHCC
>Mature Secondary Structure
MNDCSPTESPLWQWIENKVRNVLAGYGYSEVRMPIVESTPLFARAIGEVTDVVSKEMYTF
CCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEE
WDNDEQLTLRPEGTAGCVRAAIERGWIYNNEQRLWYMGPMFRHERPQKGRYRQFHQAGVE
ECCCCEEEECCCCCHHHHHHHHHHCCEECCCCEEEEECCHHHCCCCCCCHHHHHHHCCCE
VFGIPNPEIDAELIILTARLWKELGIDQHVSLQLNSIGSLEARANYRSALVKFLENHTAL
EEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
MSDEEKERLVKNPLRILDTKNQALQEVLNDAPKLLDYLDDDSREHFAHLCHLLDAMGITY
CCCHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEE
EVNPKLVRGLDYYNKTVFEWVTSALGSQGTVCGGGRYDGLVEQLGGHATQGVGFAMGLER
ECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHH
LVLLVQEVNKEINLPKAVDVYVVYSGEGATLNAFQMAERIRSELPQLGVMTHCSGGNFKK
HHHHHHHHHHHCCCCCEEEEEEEECCCCCEEHHHHHHHHHHHHCCCCCEEEECCCCCHHH
QFKRADKVEAQIALVIGESEVEQQTVVLKDLQSGAEQVIIAQADLIAELTKRF
HHHHHHHHCEEEEEEECCCHHHHHHHHHHHHHCCHHEEEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA