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The map label for this gene is purD [H]

Identifier: 126208640

GI number: 126208640

Start: 1347552

End: 1348838

Strand: Direct

Name: purD [H]

Synonym: APL_1172

Alternate gene names: 126208640

Gene position: 1347552-1348838 (Clockwise)

Preceding gene: 126208636

Following gene: 126208646

Centisome position: 59.25

GC content: 47.16

Gene sequence:

>1287_bases
ATGAATGTATTAATTATCGGTAACGGTGGCCGTGAACACGCTTTAGCTTGGAAAGTTCGTCAATCGCCTTTAGTAAAAAA
AGTGTTTGTCGCACCGGGCAATGCCGGCACCGCATTGGAAGAAGATATCAAAAATGTGGCGATAGCCGCAACCGATGTTC
CCGCATTAGTTGCTTTTGCGAAGGAAAACCAAATCGGCTTAACCATTGTCGGACCTGAAGCACCGCTTGTTATCGGTGTA
GTTGATGCATTCCGTGCGAACGGCTTAAAAATCTTTGGCCCGACTCAAGCGGCAGCACAGTTAGAAGGTTCAAAAGCCTT
TACTAAAGATTTCTTAGCTCGCCATCAGATTCCGACTGCCGAATATCAAAACTTTACCGAAATTGAACCGGCACTTGCTT
ACTTAAAACAAAAAGGTGCGCCGATTGTAATTAAAGCGGACGGTTTAGCTGCAGGTAAAGGTGTGATTGTTGCGATGACG
CTTGAAGAAGCGGAAGAAGCGGTAAAAGATATGTTATCGGGCAATGCGTTCGGCGAAGCCGGTAGCCGCGTAGTTATCGA
AGAATTTTTAGACGGTGAAGAAGCCAGTTTTATCGTGATGGTGGACGGTAAAAACGTTGAGCCGATGGCGACCAGCCAAG
ATCACAAACGTGTCGGCGAAGGCGATCAAGGTTTAAATACCGGCGGAATGGGGGCATACTCACCGGCTCCGGTCGTTACG
CCGGAAATCCATAATCGTGTTATGCAAGAAGTCATTTACCCGACCGTGAAAGGTATGGCGGCGGAAGGCAATCCATATAC
CGGTTTCCTCTATGCCGGTTTAATGATTATGCCGAACGGACAGCCGAAAGTAATCGAATTCAACTGCCGTTTCGGCGATC
CGGAAACCCAGCCGATTATGATGCGTTTAGAGTCGGATCTTGTACAACTTTGTTTAGCGGCTTGCGATGAAAAACTTGAT
ACGATTAAATCAAAATGGTGTGAACAAGCGGCGTTAGGCATCGTGCTTGCAGCGGAAGGTTATCCGGGCGATTACCGTAA
AGGCGACGAAATCAGCGGTATTCCAACTCAAGCACAAAAATCACAGAAAGTATTCCTTGCCGGTGTGGAGCAAAAAGACG
GAAAATTAGTCACTAACGGCGGGCGTGTTTTATGTGCAACCGCATTAGGTAATTCGGTGTTTGATGCACAGCAACAAGCG
TTAAAACTTGCCGAACAAATTCAATGGCAAGGACGTTTCTATCGCCGTGATATCGGCTACCGTGCGGTGGCAAGAGAAAA
AGCGTAA

Upstream 100 bases:

>100_bases
TCGGGTTTATTGATAAAAATACGAATAATAGTTCAGTATTTTGCAATCTTCTAGTACAATTACGCAAACGTTTGCTTTTA
TTAATTTATAAGGAATCCAA

Downstream 100 bases:

>100_bases
TTTGCTTTTTAAGCTAACAGCAAAAAATCAAGCGGTCTAATTGGTAAAATATTTTGCAAATTAGACCGCTTGTTATTTGT
TGATAAAACGATTAACGCTG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MNVLIIGNGGREHALAWKVRQSPLVKKVFVAPGNAGTALEEDIKNVAIAATDVPALVAFAKENQIGLTIVGPEAPLVIGV
VDAFRANGLKIFGPTQAAAQLEGSKAFTKDFLARHQIPTAEYQNFTEIEPALAYLKQKGAPIVIKADGLAAGKGVIVAMT
LEEAEEAVKDMLSGNAFGEAGSRVVIEEFLDGEEASFIVMVDGKNVEPMATSQDHKRVGEGDQGLNTGGMGAYSPAPVVT
PEIHNRVMQEVIYPTVKGMAAEGNPYTGFLYAGLMIMPNGQPKVIEFNCRFGDPETQPIMMRLESDLVQLCLAACDEKLD
TIKSKWCEQAALGIVLAAEGYPGDYRKGDEISGIPTQAQKSQKVFLAGVEQKDGKLVTNGGRVLCATALGNSVFDAQQQA
LKLAEQIQWQGRFYRRDIGYRAVAREKA

Sequences:

>Translated_428_residues
MNVLIIGNGGREHALAWKVRQSPLVKKVFVAPGNAGTALEEDIKNVAIAATDVPALVAFAKENQIGLTIVGPEAPLVIGV
VDAFRANGLKIFGPTQAAAQLEGSKAFTKDFLARHQIPTAEYQNFTEIEPALAYLKQKGAPIVIKADGLAAGKGVIVAMT
LEEAEEAVKDMLSGNAFGEAGSRVVIEEFLDGEEASFIVMVDGKNVEPMATSQDHKRVGEGDQGLNTGGMGAYSPAPVVT
PEIHNRVMQEVIYPTVKGMAAEGNPYTGFLYAGLMIMPNGQPKVIEFNCRFGDPETQPIMMRLESDLVQLCLAACDEKLD
TIKSKWCEQAALGIVLAAEGYPGDYRKGDEISGIPTQAQKSQKVFLAGVEQKDGKLVTNGGRVLCATALGNSVFDAQQQA
LKLAEQIQWQGRFYRRDIGYRAVAREKA
>Mature_428_residues
MNVLIIGNGGREHALAWKVRQSPLVKKVFVAPGNAGTALEEDIKNVAIAATDVPALVAFAKENQIGLTIVGPEAPLVIGV
VDAFRANGLKIFGPTQAAAQLEGSKAFTKDFLARHQIPTAEYQNFTEIEPALAYLKQKGAPIVIKADGLAAGKGVIVAMT
LEEAEEAVKDMLSGNAFGEAGSRVVIEEFLDGEEASFIVMVDGKNVEPMATSQDHKRVGEGDQGLNTGGMGAYSPAPVVT
PEIHNRVMQEVIYPTVKGMAAEGNPYTGFLYAGLMIMPNGQPKVIEFNCRFGDPETQPIMMRLESDLVQLCLAACDEKLD
TIKSKWCEQAALGIVLAAEGYPGDYRKGDEISGIPTQAQKSQKVFLAGVEQKDGKLVTNGGRVLCATALGNSVFDAQQQA
LKLAEQIQWQGRFYRRDIGYRAVAREKA

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=424, Percent_Identity=52.5943396226415, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI209869995, Length=424, Percent_Identity=52.5943396226415, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI209869993, Length=424, Percent_Identity=52.5943396226415, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI28416899, Length=425, Percent_Identity=52.4705882352941, Blast_Score=429, Evalue=1e-120,
Organism=Escherichia coli, GI1790438, Length=428, Percent_Identity=73.3644859813084, Blast_Score=646, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17567511, Length=426, Percent_Identity=42.9577464788732, Blast_Score=328, Evalue=3e-90,
Organism=Saccharomyces cerevisiae, GI6321203, Length=446, Percent_Identity=41.2556053811659, Blast_Score=355, Evalue=7e-99,
Organism=Drosophila melanogaster, GI24582400, Length=431, Percent_Identity=45.9396751740139, Blast_Score=343, Evalue=2e-94,
Organism=Drosophila melanogaster, GI62471619, Length=431, Percent_Identity=45.9396751740139, Blast_Score=343, Evalue=2e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45895; Mature: 45895

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLIIGNGGREHALAWKVRQSPLVKKVFVAPGNAGTALEEDIKNVAIAATDVPALVAFA
CEEEEEECCCCCCEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHEEEEECCCHHHEEEE
KENQIGLTIVGPEAPLVIGVVDAFRANGLKIFGPTQAAAQLEGSKAFTKDFLARHQIPTA
CCCCEEEEEECCCCCEEEEEHHHHHCCCEEEECCCHHHHHCCCCHHHHHHHHHHCCCCCH
EYQNFTEIEPALAYLKQKGAPIVIKADGLAAGKGVIVAMTLEEAEEAVKDMLSGNAFGEA
HCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCC
GSRVVIEEFLDGEEASFIVMVDGKNVEPMATSQDHKRVGEGDQGLNTGGMGAYSPAPVVT
CCEEEHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCC
PEIHNRVMQEVIYPTVKGMAAEGNPYTGFLYAGLMIMPNGQPKVIEFNCRFGDPETQPIM
HHHHHHHHHHHHHHHHCCEECCCCCCEEEEEECEEEEECCCCEEEEEEECCCCCCCCHHH
MRLESDLVQLCLAACDEKLDTIKSKWCEQAALGIVLAAEGYPGDYRKGDEISGIPTQAQK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCCCCC
SQKVFLAGVEQKDGKLVTNGGRVLCATALGNSVFDAQQQALKLAEQIQWQGRFYRRDIGY
CCEEEEEECCCCCCEEEECCCCEEEEEHHCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCH
RAVAREKA
HHHHCCCC
>Mature Secondary Structure
MNVLIIGNGGREHALAWKVRQSPLVKKVFVAPGNAGTALEEDIKNVAIAATDVPALVAFA
CEEEEEECCCCCCEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHEEEEECCCHHHEEEE
KENQIGLTIVGPEAPLVIGVVDAFRANGLKIFGPTQAAAQLEGSKAFTKDFLARHQIPTA
CCCCEEEEEECCCCCEEEEEHHHHHCCCEEEECCCHHHHHCCCCHHHHHHHHHHCCCCCH
EYQNFTEIEPALAYLKQKGAPIVIKADGLAAGKGVIVAMTLEEAEEAVKDMLSGNAFGEA
HCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCC
GSRVVIEEFLDGEEASFIVMVDGKNVEPMATSQDHKRVGEGDQGLNTGGMGAYSPAPVVT
CCEEEHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCC
PEIHNRVMQEVIYPTVKGMAAEGNPYTGFLYAGLMIMPNGQPKVIEFNCRFGDPETQPIM
HHHHHHHHHHHHHHHHCCEECCCCCCEEEEEECEEEEECCCCEEEEEEECCCCCCCCHHH
MRLESDLVQLCLAACDEKLDTIKSKWCEQAALGIVLAAEGYPGDYRKGDEISGIPTQAQK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCCCCC
SQKVFLAGVEQKDGKLVTNGGRVLCATALGNSVFDAQQQALKLAEQIQWQGRFYRRDIGY
CCEEEEEECCCCCCEEEECCCCEEEEEHHCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCH
RAVAREKA
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]