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The map label for this gene is metE

Identifier: 126208625

GI number: 126208625

Start: 1330700

End: 1332973

Strand: Reverse

Name: metE

Synonym: APL_1155

Alternate gene names: 126208625

Gene position: 1332973-1330700 (Counterclockwise)

Preceding gene: 126208627

Following gene: 126208624

Centisome position: 58.61

GC content: 44.11

Gene sequence:

>2274_bases
ATGACAACATTACATATCTTAGGTTTCCCACGTGTAGGGGCAAAACGTGAATTAAAATTTGCGCAGGAACGCTACTGGCG
TAAAGAATTAGCCGAACAAGATTTATTAGATCTGGCAAAAGCGTTACGTGAAAAAAATTGGCTGCATCAAGCGCAAGCCG
ATGCGGATTTTGTCACGGTCGGCGATTTTACGTTTTACGATCACATTTTAGATCTACAAGTAGCAACCGGCGCGATACCG
GCTCGTTTTGGTTTTGACAGCCAAACGCTGAGCCTTGATCAATATTTCCAATTAGCACGTGGTAATAAAGAGCAGTTTGC
GATTGAAATGACCAAATGGTTTGATACTAACTATCACTATCTTGTACCGGAATTTCAGAAAAATACCACGTTTAAGGCGA
ATCCTACGCATTATGTGAATCAAATTCGTGAAGCGAAAGCTGCCGGACATCAGGTTAAACCGGTGATCGTCGGTCCGCTC
ACATTTTTATGGCTAGGTAAAGAAAAAGGCGAAGCCTTTAACCGATTTGATTTGCTCAAGCAACTTGTGCCGATTTATTT
TGAGATTTTAACCGCACTCGCCGCTGAAGGTGTGGAATGGATTCAAATTGATGAACCGGCATTGGCATTAGATTTACCTC
AAGAATGGCTTGCGGCTTATCAAGATGTTTACGCTCAATTAGCTAAAGTGAATGCGAAGCTATTATTGGCAACTTATTTT
GGCTCGGTGGCGGAACACGCCGATTTATTAAAAGCGTTGCCGGTAGACGGTTTACACCTTGATCTCGTGCGTGCGCCGGA
ACAACTCAGTGCATTTGAAGATTATCCGAAAGTGCTTTCTGCCGGTGTGATTGATGGGCGTAATATTTGGCGAGCGAATT
TAAATCGTGTATTGGATGTGTTGGAACCGTTAAAAGCGAAATTTAATCAGCGTTTATGGATTGCACCGAGCTGTTCGCTA
TTGCATACACCTTATGATTTAGCGGTAGAAACGCAATTACAGCAAAATAATCCTGAATTATATCGCTGGTTAGCTTTTAC
CTTACAAAAAGTGGCAGAGCTGCAGGTTTTAAAACAGGCATTAAATGCGGGGCGAGATGCGGTTACAGAACAACTCAATG
ACAGCCAAACAGCCGCAGATGCTCGAGCAAACAGCAACGTGATTCATAAAGCGGAAGTCGCGCAACGTTTAGCTAACTTA
CCGGCAAATGCGGATAAGCGCCAGTCACCTTTTGCCGAGCGAATTAAATTGCAAAATAAATGGCTAAATTTACCGCTTCT
TCCGACAACCAATATCGGTTCTTTCCCGCAAACATTGGAAATTCGTCACGCCCGTGCCGCATTTAAAAAAGGTGAACTCT
CGCTTGCCGATTATGAAGCGGCAATGAAAAAGGAAATTGAATTTGTGGTACGTAAGCAGGAAGAACTGGATTTAGACGTA
CTGGTGCACGGTGAAGGGGAACGTAACGATATGGTGGAATATTTCGGTGAGTTGCTGGACGGTTTTGCTTTCACCAAATT
CGGTTGGGTACAAAGCTACGGTTCTCGTTGTGTAAAACCGCCGGTGATTTATGGTGACGTCACTCGTCCTGAACCGATGA
CGGTACGTTGGTCGCAATATGCTCAAAGCCTGACCGATAAAGTGATGAAGGGTATGCTAACCGGTCCGGTGACGATTTTA
CAATGGTCGTTTGTGCGTAACGATATTCCGCGTTCCACGGTATGTAAGCAAATTGGTGTGGCACTTTCAGATGAGGTATT
AGATCTGGAAAAAGCCGGTATTAAAGTGATTCAGATTGATGAGCCGGCGATTCGAGAGGGCTTACCGCTTAAACGTGCGG
ATTGGGATGCCTATTTACAATGGGCGGGTGAAGCGTTCCGTTTAAGTTATATGGGGGTAAAAGACGATACCCAAATCCAC
ACCCATATGTGTTATTCGGAATTTAACGATATTTTACCGGCGATTGCAGGGCTTGATGCGGATGTGATCACGATTGAAAC
CTCACGTTCGGATATGGAATTACTCACGGCGTTTGCAGATTTCAAATATCCGAATGATATCGGCCCGGGCGTTTATGATA
TTCATAGCCCTCGTGTACCGACCGCCGGCGAAATCGAGCATTTATTACGTAAGGCATTGAAGGTAATACCGAAAGAACGT
TTATGGGTGAATCCGGATTGCGGCTTAAAAACGCGCGGTTGGAAAGAAACTATTGAACAATTACAAGTGATGGTTGAAGT
AACGAAAAAATTACGTGCGGAATTAGCCGAATAA

Upstream 100 bases:

>100_bases
ATGAAGTTGCATAATATTAACGGTTCTTGCATTATTTTGATATCGACTTAGCTCATTGCATAGTTAAGTCATCCGAATCA
GAACATTGCAGGAGTGCAGT

Downstream 100 bases:

>100_bases
CTCGACAAAATCGCATTTAATTTTTCATAAAAGCAAGCGGTTTAATTTGCAAAAAAATAGGCAAATTAGACCGCTTGCGA
CAATTATTTGTTAGAATATC

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme

Number of amino acids: Translated: 757; Mature: 756

Protein sequence:

>757_residues
MTTLHILGFPRVGAKRELKFAQERYWRKELAEQDLLDLAKALREKNWLHQAQADADFVTVGDFTFYDHILDLQVATGAIP
ARFGFDSQTLSLDQYFQLARGNKEQFAIEMTKWFDTNYHYLVPEFQKNTTFKANPTHYVNQIREAKAAGHQVKPVIVGPL
TFLWLGKEKGEAFNRFDLLKQLVPIYFEILTALAAEGVEWIQIDEPALALDLPQEWLAAYQDVYAQLAKVNAKLLLATYF
GSVAEHADLLKALPVDGLHLDLVRAPEQLSAFEDYPKVLSAGVIDGRNIWRANLNRVLDVLEPLKAKFNQRLWIAPSCSL
LHTPYDLAVETQLQQNNPELYRWLAFTLQKVAELQVLKQALNAGRDAVTEQLNDSQTAADARANSNVIHKAEVAQRLANL
PANADKRQSPFAERIKLQNKWLNLPLLPTTNIGSFPQTLEIRHARAAFKKGELSLADYEAAMKKEIEFVVRKQEELDLDV
LVHGEGERNDMVEYFGELLDGFAFTKFGWVQSYGSRCVKPPVIYGDVTRPEPMTVRWSQYAQSLTDKVMKGMLTGPVTIL
QWSFVRNDIPRSTVCKQIGVALSDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLQWAGEAFRLSYMGVKDDTQIH
THMCYSEFNDILPAIAGLDADVITIETSRSDMELLTAFADFKYPNDIGPGVYDIHSPRVPTAGEIEHLLRKALKVIPKER
LWVNPDCGLKTRGWKETIEQLQVMVEVTKKLRAELAE

Sequences:

>Translated_757_residues
MTTLHILGFPRVGAKRELKFAQERYWRKELAEQDLLDLAKALREKNWLHQAQADADFVTVGDFTFYDHILDLQVATGAIP
ARFGFDSQTLSLDQYFQLARGNKEQFAIEMTKWFDTNYHYLVPEFQKNTTFKANPTHYVNQIREAKAAGHQVKPVIVGPL
TFLWLGKEKGEAFNRFDLLKQLVPIYFEILTALAAEGVEWIQIDEPALALDLPQEWLAAYQDVYAQLAKVNAKLLLATYF
GSVAEHADLLKALPVDGLHLDLVRAPEQLSAFEDYPKVLSAGVIDGRNIWRANLNRVLDVLEPLKAKFNQRLWIAPSCSL
LHTPYDLAVETQLQQNNPELYRWLAFTLQKVAELQVLKQALNAGRDAVTEQLNDSQTAADARANSNVIHKAEVAQRLANL
PANADKRQSPFAERIKLQNKWLNLPLLPTTNIGSFPQTLEIRHARAAFKKGELSLADYEAAMKKEIEFVVRKQEELDLDV
LVHGEGERNDMVEYFGELLDGFAFTKFGWVQSYGSRCVKPPVIYGDVTRPEPMTVRWSQYAQSLTDKVMKGMLTGPVTIL
QWSFVRNDIPRSTVCKQIGVALSDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLQWAGEAFRLSYMGVKDDTQIH
THMCYSEFNDILPAIAGLDADVITIETSRSDMELLTAFADFKYPNDIGPGVYDIHSPRVPTAGEIEHLLRKALKVIPKER
LWVNPDCGLKTRGWKETIEQLQVMVEVTKKLRAELAE
>Mature_756_residues
TTLHILGFPRVGAKRELKFAQERYWRKELAEQDLLDLAKALREKNWLHQAQADADFVTVGDFTFYDHILDLQVATGAIPA
RFGFDSQTLSLDQYFQLARGNKEQFAIEMTKWFDTNYHYLVPEFQKNTTFKANPTHYVNQIREAKAAGHQVKPVIVGPLT
FLWLGKEKGEAFNRFDLLKQLVPIYFEILTALAAEGVEWIQIDEPALALDLPQEWLAAYQDVYAQLAKVNAKLLLATYFG
SVAEHADLLKALPVDGLHLDLVRAPEQLSAFEDYPKVLSAGVIDGRNIWRANLNRVLDVLEPLKAKFNQRLWIAPSCSLL
HTPYDLAVETQLQQNNPELYRWLAFTLQKVAELQVLKQALNAGRDAVTEQLNDSQTAADARANSNVIHKAEVAQRLANLP
ANADKRQSPFAERIKLQNKWLNLPLLPTTNIGSFPQTLEIRHARAAFKKGELSLADYEAAMKKEIEFVVRKQEELDLDVL
VHGEGERNDMVEYFGELLDGFAFTKFGWVQSYGSRCVKPPVIYGDVTRPEPMTVRWSQYAQSLTDKVMKGMLTGPVTILQ
WSFVRNDIPRSTVCKQIGVALSDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLQWAGEAFRLSYMGVKDDTQIHT
HMCYSEFNDILPAIAGLDADVITIETSRSDMELLTAFADFKYPNDIGPGVYDIHSPRVPTAGEIEHLLRKALKVIPKERL
WVNPDCGLKTRGWKETIEQLQVMVEVTKKLRAELAE

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family

Homologues:

Organism=Escherichia coli, GI2367304, Length=760, Percent_Identity=54.8684210526316, Blast_Score=828, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=775, Percent_Identity=45.6774193548387, Blast_Score=654, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METE_ACTP2 (A3N1F9)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053850.1
- ProteinModelPortal:   A3N1F9
- SMR:   A3N1F9
- STRING:   A3N1F9
- GeneID:   4849095
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1155
- eggNOG:   COG0620
- HOGENOM:   HBG287495
- OMA:   RFGWVQS
- PhylomeDB:   A3N1F9
- ProtClustDB:   PRK05222
- BioCyc:   APLE416269:APL_1155-MONOMER
- HAMAP:   MF_00172
- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629
- PIRSF:   PIRSF000382
- TIGRFAMs:   TIGR01371

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2

EC number: =2.1.1.14

Molecular weight: Translated: 85887; Mature: 85756

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLHILGFPRVGAKRELKFAQERYWRKELAEQDLLDLAKALREKNWLHQAQADADFVTV
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
GDFTFYDHILDLQVATGAIPARFGFDSQTLSLDQYFQLARGNKEQFAIEMTKWFDTNYHY
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHEEEEEHHHHCCCCEE
LVPEFQKNTTFKANPTHYVNQIREAKAAGHQVKPVIVGPLTFLWLGKEKGEAFNRFDLLK
ECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCEEEEEECCCCCCHHHHHHHHH
QLVPIYFEILTALAAEGVEWIQIDEPALALDLPQEWLAAYQDVYAQLAKVNAKLLLATYF
HHHHHHHHHHHHHHHCCCEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSVAEHADLLKALPVDGLHLDLVRAPEQLSAFEDYPKVLSAGVIDGRNIWRANLNRVLDV
HHHHHHHHHHHHCCCCCCEEHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LEPLKAKFNQRLWIAPSCSLLHTPYDLAVETQLQQNNPELYRWLAFTLQKVAELQVLKQA
HHHHHHHHCCCEEECCCCCCCCCCCCCEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LNAGRDAVTEQLNDSQTAADARANSNVIHKAEVAQRLANLPANADKRQSPFAERIKLQNK
HHCCHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
WLNLPLLPTTNIGSFPQTLEIRHARAAFKKGELSLADYEAAMKKEIEFVVRKQEELDLDV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEE
LVHGEGERNDMVEYFGELLDGFAFTKFGWVQSYGSRCVKPPVIYGDVTRPEPMTVRWSQY
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCEEEHHHH
AQSLTDKVMKGMLTGPVTILQWSFVRNDIPRSTVCKQIGVALSDEVLDLEKAGIKVIQID
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHCCEEEEEEC
EPAIREGLPLKRADWDAYLQWAGEAFRLSYMGVKDDTQIHTHMCYSEFNDILPAIAGLDA
CHHHHCCCCCCCCCHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCC
DVITIETSRSDMELLTAFADFKYPNDIGPGVYDIHSPRVPTAGEIEHLLRKALKVIPKER
CEEEEECCCCHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCC
LWVNPDCGLKTRGWKETIEQLQVMVEVTKKLRAELAE
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTLHILGFPRVGAKRELKFAQERYWRKELAEQDLLDLAKALREKNWLHQAQADADFVTV
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
GDFTFYDHILDLQVATGAIPARFGFDSQTLSLDQYFQLARGNKEQFAIEMTKWFDTNYHY
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHEEEEEHHHHCCCCEE
LVPEFQKNTTFKANPTHYVNQIREAKAAGHQVKPVIVGPLTFLWLGKEKGEAFNRFDLLK
ECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCEEEEEECCCCCCHHHHHHHHH
QLVPIYFEILTALAAEGVEWIQIDEPALALDLPQEWLAAYQDVYAQLAKVNAKLLLATYF
HHHHHHHHHHHHHHHCCCEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSVAEHADLLKALPVDGLHLDLVRAPEQLSAFEDYPKVLSAGVIDGRNIWRANLNRVLDV
HHHHHHHHHHHHCCCCCCEEHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LEPLKAKFNQRLWIAPSCSLLHTPYDLAVETQLQQNNPELYRWLAFTLQKVAELQVLKQA
HHHHHHHHCCCEEECCCCCCCCCCCCCEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LNAGRDAVTEQLNDSQTAADARANSNVIHKAEVAQRLANLPANADKRQSPFAERIKLQNK
HHCCHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
WLNLPLLPTTNIGSFPQTLEIRHARAAFKKGELSLADYEAAMKKEIEFVVRKQEELDLDV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEE
LVHGEGERNDMVEYFGELLDGFAFTKFGWVQSYGSRCVKPPVIYGDVTRPEPMTVRWSQY
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCEEEHHHH
AQSLTDKVMKGMLTGPVTILQWSFVRNDIPRSTVCKQIGVALSDEVLDLEKAGIKVIQID
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHCCEEEEEEC
EPAIREGLPLKRADWDAYLQWAGEAFRLSYMGVKDDTQIHTHMCYSEFNDILPAIAGLDA
CHHHHCCCCCCCCCHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCC
DVITIETSRSDMELLTAFADFKYPNDIGPGVYDIHSPRVPTAGEIEHLLRKALKVIPKER
CEEEEECCCCHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCC
LWVNPDCGLKTRGWKETIEQLQVMVEVTKKLRAELAE
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA