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The map label for this gene is amiB [H]

Identifier: 126208606

GI number: 126208606

Start: 1312068

End: 1313213

Strand: Direct

Name: amiB [H]

Synonym: APL_1136

Alternate gene names: 126208606

Gene position: 1312068-1313213 (Clockwise)

Preceding gene: 126208605

Following gene: 126208616

Centisome position: 57.69

GC content: 39.44

Gene sequence:

>1146_bases
ATGAACAAGTTAATAAATTATATTGCAGTTAGTTTATTTGGCTTAATGATTAGCTTAACCAGTTATGCAAAAGTCGTCGT
CGTTATCGATGCGGGACACGGCGGTAAAGATCCGGGTGCAATCGGTAAAACCTTAGGAATTAAGGAAAAAGAAGTGACGC
TCGGTATTTCGAAAGAATTAAAAGCATTACTTGATGCGGATCCTAATTTTAAAGCGGTCATGACACGTAAGAGCGACTAC
TTTATTCAGTTACCGAATCGTACGGAAATCGCCAGAAGAAATAAGGCGAATTACCTTATTTCCATCCATGCGGATTCATC
ACCTAATTCGAGTAGCCAAAAAGGCGCTTCCGTATGGGTGCTTTCCAATCGCCGAGCCAGTGACGAAATGGGGAAATGGC
TTGAGGATCACGAAAAACAATCCGAATTGTTAGGCGGTGCGGGATCGGTATTATCCAATAATAACGAACGCTATCTGAAT
CAAACCGTGTTGGATTTACAATTTTCTCATTCTCAACGTTCCGGTTATGAATTAGGAAAAAGTATTCTCTCGCATATGGG
AAATATCACGACATTAGCCAAAAGCGCTCCTCAGCATGCCAGTTTAAGCGTATTACGTTCGCCGGATATTACATCCGTAT
TAGTTGAAACGGGATTCCTATCCAATTCGACGGAAGAGCAACAGCTGGCAAATCCGGCTTATCGTCGTAAAATCGCTCGA
GCGATTTATAACGGCTTAGTCGCTTATCGTGCTCGTTATACCAATGGTTCGCCTAAAGCAACAGCTAGTACCGAAACGAA
GAAAGAACAATCCGTCGCTAAAAAACAAAATAGCGAAAAAGCAGATAAATCGGTAAAAAAAGATAATAAATCCGATAAAG
CAAATAATGCTATTAAAGATAAAAAATCAGAGGGGAAAGAGAAACAAAACAAAACACAAACTAAACAGGGCGAAAAGTTA
AAAGAAGAATCTTCGTCTAAACGCCCGGAAATTCAAAAATCAACCACCGGTTACCATATTGTTCAGCAAGACGAAACGCT
TTACTCTATTGCTAGAACATATAAAACGACACCGGAAAAATTAAGTAAACTCAACAATATCAAGAACAACAATATTACCG
TCGGCAAAAAATTAAAAGTACGGTAA

Upstream 100 bases:

>100_bases
AGCTTGGACGTAATCTCACCTTGCAACCTCAAAATGAAATCGGTGATCAAATTATTGCAAAATTTCTGAATAACTAGACC
GCTTAACCAAAAGGAAAAAA

Downstream 100 bases:

>100_bases
AAATAAATAGCGGTCATTTTTACAAAATAAATTGCAAAAATGACCGCTATTGCTTGGTATTAAATACTTACGGACGGATT
AACGCCATGCTTTAAATTGA

Product: putative N-acetylmuramoyl-L-alanine amidase AmiB precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MNKLINYIAVSLFGLMISLTSYAKVVVVIDAGHGGKDPGAIGKTLGIKEKEVTLGISKELKALLDADPNFKAVMTRKSDY
FIQLPNRTEIARRNKANYLISIHADSSPNSSSQKGASVWVLSNRRASDEMGKWLEDHEKQSELLGGAGSVLSNNNERYLN
QTVLDLQFSHSQRSGYELGKSILSHMGNITTLAKSAPQHASLSVLRSPDITSVLVETGFLSNSTEEQQLANPAYRRKIAR
AIYNGLVAYRARYTNGSPKATASTETKKEQSVAKKQNSEKADKSVKKDNKSDKANNAIKDKKSEGKEKQNKTQTKQGEKL
KEESSSKRPEIQKSTTGYHIVQQDETLYSIARTYKTTPEKLSKLNNIKNNNITVGKKLKVR

Sequences:

>Translated_381_residues
MNKLINYIAVSLFGLMISLTSYAKVVVVIDAGHGGKDPGAIGKTLGIKEKEVTLGISKELKALLDADPNFKAVMTRKSDY
FIQLPNRTEIARRNKANYLISIHADSSPNSSSQKGASVWVLSNRRASDEMGKWLEDHEKQSELLGGAGSVLSNNNERYLN
QTVLDLQFSHSQRSGYELGKSILSHMGNITTLAKSAPQHASLSVLRSPDITSVLVETGFLSNSTEEQQLANPAYRRKIAR
AIYNGLVAYRARYTNGSPKATASTETKKEQSVAKKQNSEKADKSVKKDNKSDKANNAIKDKKSEGKEKQNKTQTKQGEKL
KEESSSKRPEIQKSTTGYHIVQQDETLYSIARTYKTTPEKLSKLNNIKNNNITVGKKLKVR
>Mature_381_residues
MNKLINYIAVSLFGLMISLTSYAKVVVVIDAGHGGKDPGAIGKTLGIKEKEVTLGISKELKALLDADPNFKAVMTRKSDY
FIQLPNRTEIARRNKANYLISIHADSSPNSSSQKGASVWVLSNRRASDEMGKWLEDHEKQSELLGGAGSVLSNNNERYLN
QTVLDLQFSHSQRSGYELGKSILSHMGNITTLAKSAPQHASLSVLRSPDITSVLVETGFLSNSTEEQQLANPAYRRKIAR
AIYNGLVAYRARYTNGSPKATASTETKKEQSVAKKQNSEKADKSVKKDNKSDKANNAIKDKKSEGKEKQNKTQTKQGEKL
KEESSSKRPEIQKSTTGYHIVQQDETLYSIARTYKTTPEKLSKLNNIKNNNITVGKKLKVR

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1790611, Length=255, Percent_Identity=54.5098039215686, Blast_Score=284, Evalue=6e-78,
Organism=Escherichia coli, GI87082163, Length=227, Percent_Identity=37.0044052863436, Blast_Score=160, Evalue=9e-41,
Organism=Escherichia coli, GI1788776, Length=235, Percent_Identity=39.1489361702128, Blast_Score=155, Evalue=6e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR018392
- InterPro:   IPR002482 [H]

Pfam domain/function: PF01520 Amidase_3; PF01476 LysM [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 42134; Mature: 42134

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLINYIAVSLFGLMISLTSYAKVVVVIDAGHGGKDPGAIGKTLGIKEKEVTLGISKEL
CHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCHHCCCCCHHEEECHHHHH
KALLDADPNFKAVMTRKSDYFIQLPNRTEIARRNKANYLISIHADSSPNSSSQKGASVWV
HHHHCCCCCCEEEEEECCCEEEECCCCHHHHHCCCCCEEEEEECCCCCCCCCCCCCEEEE
LSNRRASDEMGKWLEDHEKQSELLGGAGSVLSNNNERYLNQTVLDLQFSHSQRSGYELGK
EECCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHEEEEECCCCCHHHHHHH
SILSHMGNITTLAKSAPQHASLSVLRSPDITSVLVETGFLSNSTEEQQLANPAYRRKIAR
HHHHHCCCHHHHHHCCCCCCCHHEECCCCHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHH
AIYNGLVAYRARYTNGSPKATASTETKKEQSVAKKQNSEKADKSVKKDNKSDKANNAIKD
HHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHC
KKSEGKEKQNKTQTKQGEKLKEESSSKRPEIQKSTTGYHIVQQDETLYSIARTYKTTPEK
CHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCHHH
LSKLNNIKNNNITVGKKLKVR
HHHHHCCCCCCEEECCEEECC
>Mature Secondary Structure
MNKLINYIAVSLFGLMISLTSYAKVVVVIDAGHGGKDPGAIGKTLGIKEKEVTLGISKEL
CHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCHHCCCCCHHEEECHHHHH
KALLDADPNFKAVMTRKSDYFIQLPNRTEIARRNKANYLISIHADSSPNSSSQKGASVWV
HHHHCCCCCCEEEEEECCCEEEECCCCHHHHHCCCCCEEEEEECCCCCCCCCCCCCEEEE
LSNRRASDEMGKWLEDHEKQSELLGGAGSVLSNNNERYLNQTVLDLQFSHSQRSGYELGK
EECCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHEEEEECCCCCHHHHHHH
SILSHMGNITTLAKSAPQHASLSVLRSPDITSVLVETGFLSNSTEEQQLANPAYRRKIAR
HHHHHCCCHHHHHHCCCCCCCHHEECCCCHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHH
AIYNGLVAYRARYTNGSPKATASTETKKEQSVAKKQNSEKADKSVKKDNKSDKANNAIKD
HHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHC
KKSEGKEKQNKTQTKQGEKLKEESSSKRPEIQKSTTGYHIVQQDETLYSIARTYKTTPEK
CHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCHHH
LSKLNNIKNNNITVGKKLKVR
HHHHHCCCCCCEEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]