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The map label for this gene is priA [H]

Identifier: 126208506

GI number: 126208506

Start: 1196311

End: 1198527

Strand: Reverse

Name: priA [H]

Synonym: APL_1032

Alternate gene names: 126208506

Gene position: 1198527-1196311 (Counterclockwise)

Preceding gene: 126208507

Following gene: 126208504

Centisome position: 52.69

GC content: 44.38

Gene sequence:

>2217_bases
ATGTCGAAGCTAGTTAGAGTGGCATTACCCGTACCGTTACATCGTTATTTTGATTATTTACTGCCGCCTTTTTTGTCGGC
GGTCAAAGGCGTGCGTGTTGAAGTGCCTTTCGGTAATCAAACGAAAATCGGCGTGGTGGTGGATTTCCCTGAAACAAGCG
ATGTGCCTGAAGATAAGCTCAAACCGATTAAAGCGGTATTAGATTTAGAACCGATCTTTGATGAGGAAATTTGGACACTG
CTTCGTTGGGCGGCTCGTTATTATCATGCCCCTATCGGTGAAGTGGCGATCAATGCCTTGCCGGTGAAATTGCGTAACGG
CGATAGTACCGAAAGATCGCAACCGGATTACTTTACTGTTACTGAGGCAGGGCGAGCAGCATTGCTCTCGGGTGAGAACA
AGCGGGCAAAAAAACAGCAAGATTTACTGGAGAAACTGGCAGAAATTGCAAGTTTCTTTGAAAAACCGACCGCTTGTAGC
CAATCGGCTTGGAAAGGGCTATTGGAAAAAAACTATATTGAACCGGTTAATGTACCGTTTGAACCGCAACATTGGCAAAA
AAAATTGCAAAATCGACCGCTTGTAAACTTACATAATCGATTGGTGTTGAATAAACAGCAAAGCTTAGTGGTGAGCCGTT
TAACGGTACAAAACGGTTTCGAAGCTTTTTTATTAAATGGCGTAACCGGTTCAGGCAAAACCGAAGTATATTTGCAAGTG
ATCGAAGAGGTGTTAAAGCGAGGCGAGCAAGTATTGGTGTTAGTACCGGAAATCGGGCTGACCCCGCAAACGGTGCAACG
TTTTAAAGCCCGTTTTAATGTTGAAATTGATGTTCTTCATTCGAATATGAATGAAACAGAGCGATTAAATGCTTGGTTGC
GAGCAAAAAACGGCGAAAGTGCGATTGTGATCGGCACGCGTTCGGCATTGTTCAGCCAGTTTGAGAAATTGGGCTTAATC
ATTATTGATGAAGAACACGATAGTTCTTTCAAACAACAAGACGGCTGGCGTTATCACGCAAGAGATTTGGCGATTTTACG
GGCGAAAAATTTACATATCCCGATTATTTTGGGTTCTGCAACGCCAAGTTTGGAAAGCGTACAAAACGTTCAAAACGGTA
AGTTTATTGAGCTATCTTTAACCGCTCGTGCCGGTAATGCTCAACAGGCGAAACAGCAATTGATTGATTTAAAAACACAG
CGTATTACCGCCGGACTTTCCGACCGCTTATTAGCGATGATGAAACGGCATTTGGAGCAAGGTAATCAGGTGGTGTTGTT
TTTAAATCGCCGTGGCTTTGCACCGGTTTTACTTTGTCACGAGTGTGGCTGGATGTGTGAATGTGACGCTTGTGAAAAGC
CTTACACTTATCATCAGAAACAGAAAATCTTGCGTTGTCATCATTGTGCTTCACAGCGAGTGATCCCTCGCCAGTGCGGG
CATTGCGGCTCAACCAATCTGATTACCACCGGAGTGGGGACGGAGCAGTTGGAGCAAGTGTTAAGTGAGCAATTCCCAAC
TTACCAAATTAGCCGTATCGACCGAGATTCCACCGCTCGTAAAGGGGCATTGGAAAATCACTTAAATGATATCCGAGAAG
GTAAAAGCCAAATCCTTATCGGCACGCAAATGTTAGCGAAAGGGCATCATTTTCCGAATGTAACGTTAGTGGCGATTGTG
AATGTAGATTCGGCATTGTTTTCGACCGATTTCCGTGCGGAAGAACGTTTGGCACAGCTTTATGTGCAAGTGGCGGGACG
TGCCGGACGGGCGGAAAAGCAGGGCGAAGTGGTATTACAAACTCATTATCCGGAACATCCGTTGCTCAAGACTTTGTTAG
AACAAGGTTATTTGGCGTTTGCACAAGAAGCGTTAAAAATGCGTAAAGTGATGGGCTTGCCGCCGTTTGCTGCGCAAGTG
TTGTTACGGGCAACCGGCAAAGATAATCAGCAAGTGGTAAATCTTTTGCAAAATTTGACCGCTTATTTTCAGCAAAAATG
TACGGAACTCGGTTTGCAGGATATCCAGCTTCTACCGCCTTTTTCCGCACCGATGGCTAAAAAAGCGGGGCATTACCGCT
GGCTTTTATTAATTCAACATCGCTCAAGAGGCGTATTACAACAGTTGATCGATCAATTTGATTTGGATAAAGCCAATTTA
GGCTTACAAAATAATATTCGTTTAACCTTAGATATTGATCCGCAAGAAATCGGCTAA

Upstream 100 bases:

>100_bases
GCCGGTAAAGCTGTAACAAGCGGTTAAATTTTAAGATTTTTTTGCAAAACCCCAAAGGGCAATAGATAATAAGCTATTGT
CCTTTCGTTTTTTATTGATT

Downstream 100 bases:

>100_bases
CAGCGGATTCATACGACAAGTGGTCTGATTTTCCATATTTTTTACAAAAAATAACGAAAATCAGACCGCTTCATTTAGGC
TATTTTTCTTTATTTAGGCT

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 738; Mature: 737

Protein sequence:

>738_residues
MSKLVRVALPVPLHRYFDYLLPPFLSAVKGVRVEVPFGNQTKIGVVVDFPETSDVPEDKLKPIKAVLDLEPIFDEEIWTL
LRWAARYYHAPIGEVAINALPVKLRNGDSTERSQPDYFTVTEAGRAALLSGENKRAKKQQDLLEKLAEIASFFEKPTACS
QSAWKGLLEKNYIEPVNVPFEPQHWQKKLQNRPLVNLHNRLVLNKQQSLVVSRLTVQNGFEAFLLNGVTGSGKTEVYLQV
IEEVLKRGEQVLVLVPEIGLTPQTVQRFKARFNVEIDVLHSNMNETERLNAWLRAKNGESAIVIGTRSALFSQFEKLGLI
IIDEEHDSSFKQQDGWRYHARDLAILRAKNLHIPIILGSATPSLESVQNVQNGKFIELSLTARAGNAQQAKQQLIDLKTQ
RITAGLSDRLLAMMKRHLEQGNQVVLFLNRRGFAPVLLCHECGWMCECDACEKPYTYHQKQKILRCHHCASQRVIPRQCG
HCGSTNLITTGVGTEQLEQVLSEQFPTYQISRIDRDSTARKGALENHLNDIREGKSQILIGTQMLAKGHHFPNVTLVAIV
NVDSALFSTDFRAEERLAQLYVQVAGRAGRAEKQGEVVLQTHYPEHPLLKTLLEQGYLAFAQEALKMRKVMGLPPFAAQV
LLRATGKDNQQVVNLLQNLTAYFQQKCTELGLQDIQLLPPFSAPMAKKAGHYRWLLLIQHRSRGVLQQLIDQFDLDKANL
GLQNNIRLTLDIDPQEIG

Sequences:

>Translated_738_residues
MSKLVRVALPVPLHRYFDYLLPPFLSAVKGVRVEVPFGNQTKIGVVVDFPETSDVPEDKLKPIKAVLDLEPIFDEEIWTL
LRWAARYYHAPIGEVAINALPVKLRNGDSTERSQPDYFTVTEAGRAALLSGENKRAKKQQDLLEKLAEIASFFEKPTACS
QSAWKGLLEKNYIEPVNVPFEPQHWQKKLQNRPLVNLHNRLVLNKQQSLVVSRLTVQNGFEAFLLNGVTGSGKTEVYLQV
IEEVLKRGEQVLVLVPEIGLTPQTVQRFKARFNVEIDVLHSNMNETERLNAWLRAKNGESAIVIGTRSALFSQFEKLGLI
IIDEEHDSSFKQQDGWRYHARDLAILRAKNLHIPIILGSATPSLESVQNVQNGKFIELSLTARAGNAQQAKQQLIDLKTQ
RITAGLSDRLLAMMKRHLEQGNQVVLFLNRRGFAPVLLCHECGWMCECDACEKPYTYHQKQKILRCHHCASQRVIPRQCG
HCGSTNLITTGVGTEQLEQVLSEQFPTYQISRIDRDSTARKGALENHLNDIREGKSQILIGTQMLAKGHHFPNVTLVAIV
NVDSALFSTDFRAEERLAQLYVQVAGRAGRAEKQGEVVLQTHYPEHPLLKTLLEQGYLAFAQEALKMRKVMGLPPFAAQV
LLRATGKDNQQVVNLLQNLTAYFQQKCTELGLQDIQLLPPFSAPMAKKAGHYRWLLLIQHRSRGVLQQLIDQFDLDKANL
GLQNNIRLTLDIDPQEIG
>Mature_737_residues
SKLVRVALPVPLHRYFDYLLPPFLSAVKGVRVEVPFGNQTKIGVVVDFPETSDVPEDKLKPIKAVLDLEPIFDEEIWTLL
RWAARYYHAPIGEVAINALPVKLRNGDSTERSQPDYFTVTEAGRAALLSGENKRAKKQQDLLEKLAEIASFFEKPTACSQ
SAWKGLLEKNYIEPVNVPFEPQHWQKKLQNRPLVNLHNRLVLNKQQSLVVSRLTVQNGFEAFLLNGVTGSGKTEVYLQVI
EEVLKRGEQVLVLVPEIGLTPQTVQRFKARFNVEIDVLHSNMNETERLNAWLRAKNGESAIVIGTRSALFSQFEKLGLII
IDEEHDSSFKQQDGWRYHARDLAILRAKNLHIPIILGSATPSLESVQNVQNGKFIELSLTARAGNAQQAKQQLIDLKTQR
ITAGLSDRLLAMMKRHLEQGNQVVLFLNRRGFAPVLLCHECGWMCECDACEKPYTYHQKQKILRCHHCASQRVIPRQCGH
CGSTNLITTGVGTEQLEQVLSEQFPTYQISRIDRDSTARKGALENHLNDIREGKSQILIGTQMLAKGHHFPNVTLVAIVN
VDSALFSTDFRAEERLAQLYVQVAGRAGRAEKQGEVVLQTHYPEHPLLKTLLEQGYLAFAQEALKMRKVMGLPPFAAQVL
LRATGKDNQQVVNLLQNLTAYFQQKCTELGLQDIQLLPPFSAPMAKKAGHYRWLLLIQHRSRGVLQQLIDQFDLDKANLG
LQNNIRLTLDIDPQEIG

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=743, Percent_Identity=52.4899057873486, Blast_Score=751, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 83287; Mature: 83156

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLVRVALPVPLHRYFDYLLPPFLSAVKGVRVEVPFGNQTKIGVVVDFPETSDVPEDKL
CCCCEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEEECCCCCCCCHHHH
KPIKAVLDLEPIFDEEIWTLLRWAARYYHAPIGEVAINALPVKLRNGDSTERSQPDYFTV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHEEEEEEECCCCCCCCCCCCEEEE
TEAGRAALLSGENKRAKKQQDLLEKLAEIASFFEKPTACSQSAWKGLLEKNYIEPVNVPF
ECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCC
EPQHWQKKLQNRPLVNLHNRLVLNKQQSLVVSRLTVQNGFEAFLLNGVTGSGKTEVYLQV
CCHHHHHHHCCCCCEECCCCEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHH
IEEVLKRGEQVLVLVPEIGLTPQTVQRFKARFNVEIDVLHSNMNETERLNAWLRAKNGES
HHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHEECCCCE
AIVIGTRSALFSQFEKLGLIIIDEEHDSSFKQQDGWRYHARDLAILRAKNLHIPIILGSA
EEEEECHHHHHHHHHHCCEEEEECCCCCCCHHCCCCEEHHHCEEEEEECCCEEEEEECCC
TPSLESVQNVQNGKFIELSLTARAGNAQQAKQQLIDLKTQRITAGLSDRLLAMMKRHLEQ
CCCHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
GNQVVLFLNRRGFAPVLLCHECGWMCECDACEKPYTYHQKQKILRCHHCASQRVIPRQCG
CCEEEEEEECCCCCCEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHCC
HCGSTNLITTGVGTEQLEQVLSEQFPTYQISRIDRDSTARKGALENHLNDIREGKSQILI
CCCCCCEEEECCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEE
GTQMLAKGHHFPNVTLVAIVNVDSALFSTDFRAEERLAQLYVQVAGRAGRAEKQGEVVLQ
EHHHHHCCCCCCCEEEEEEEECCHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
THYPEHPLLKTLLEQGYLAFAQEALKMRKVMGLPPFAAQVLLRATGKDNQQVVNLLQNLT
ECCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHH
AYFQQKCTELGLQDIQLLPPFSAPMAKKAGHYRWLLLIQHRSRGVLQQLIDQFDLDKANL
HHHHHHHHHCCCHHEEECCCCCCCHHHCCCCEEEEEEEECCCHHHHHHHHHHHCCCHHCC
GLQNNIRLTLDIDPQEIG
CCCCCEEEEEECCHHHCC
>Mature Secondary Structure 
SKLVRVALPVPLHRYFDYLLPPFLSAVKGVRVEVPFGNQTKIGVVVDFPETSDVPEDKL
CCCEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEEECCCCCCCCHHHH
KPIKAVLDLEPIFDEEIWTLLRWAARYYHAPIGEVAINALPVKLRNGDSTERSQPDYFTV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHEEEEEEECCCCCCCCCCCCEEEE
TEAGRAALLSGENKRAKKQQDLLEKLAEIASFFEKPTACSQSAWKGLLEKNYIEPVNVPF
ECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCC
EPQHWQKKLQNRPLVNLHNRLVLNKQQSLVVSRLTVQNGFEAFLLNGVTGSGKTEVYLQV
CCHHHHHHHCCCCCEECCCCEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHH
IEEVLKRGEQVLVLVPEIGLTPQTVQRFKARFNVEIDVLHSNMNETERLNAWLRAKNGES
HHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHEECCCCE
AIVIGTRSALFSQFEKLGLIIIDEEHDSSFKQQDGWRYHARDLAILRAKNLHIPIILGSA
EEEEECHHHHHHHHHHCCEEEEECCCCCCCHHCCCCEEHHHCEEEEEECCCEEEEEECCC
TPSLESVQNVQNGKFIELSLTARAGNAQQAKQQLIDLKTQRITAGLSDRLLAMMKRHLEQ
CCCHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
GNQVVLFLNRRGFAPVLLCHECGWMCECDACEKPYTYHQKQKILRCHHCASQRVIPRQCG
CCEEEEEEECCCCCCEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHCC
HCGSTNLITTGVGTEQLEQVLSEQFPTYQISRIDRDSTARKGALENHLNDIREGKSQILI
CCCCCCEEEECCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEE
GTQMLAKGHHFPNVTLVAIVNVDSALFSTDFRAEERLAQLYVQVAGRAGRAEKQGEVVLQ
EHHHHHCCCCCCCEEEEEEEECCHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
THYPEHPLLKTLLEQGYLAFAQEALKMRKVMGLPPFAAQVLLRATGKDNQQVVNLLQNLT
ECCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHH
AYFQQKCTELGLQDIQLLPPFSAPMAKKAGHYRWLLLIQHRSRGVLQQLIDQFDLDKANL
HHHHHHHHHCCCHHEEECCCCCCCHHHCCCCEEEEEEEECCCHHHHHHHHHHHCCCHHCC
GLQNNIRLTLDIDPQEIG
CCCCCEEEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]