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The map label for this gene is eda [H]

Identifier: 126208492

GI number: 126208492

Start: 1184113

End: 1184754

Strand: Reverse

Name: eda [H]

Synonym: APL_1018

Alternate gene names: 126208492

Gene position: 1184754-1184113 (Counterclockwise)

Preceding gene: 126208493

Following gene: 126208490

Centisome position: 52.09

GC content: 47.35

Gene sequence:

>642_bases
ATGAAATATACAACAGAACAAATTATTGAAAAACTTCGTCAGTTAAAAGTCGTGCCGGTAATTGCGGTAGAACAAGCGGA
AGATATTTTACCGTTAGTTAAGACGTTAGCGGAAAACGGTTTACCGGTGGCGGAGATTACTTTCCGCTCGGCTGCGGCGG
AAGAGGCGATTCGCTTAGTATGCCAGCATTATCCGGACGTATTGATTGCCGCCGGTACGGTATTAACCGCCGAGCAAGTG
GTCAAAGCGAAAAATGCCGGTGCGGATTTTGTCGTGACACCGGGCTTTAACCCGACTATCGTGAAACTGTGCCAAGATTT
AGATTTTCCGATTACGCCGGGGGTAAACAATCCGATGTCGATTGAAGCGGCATTATCGTTAGGCATTGAAGCGGTCAAAT
TTTTCCCGGCGGAAGCCAGCGGCGGTGTGAAAATGATTAAAGTCTTACTCGGTCCTTATGGTAATCTGCAAATTATGCCG
ACCGGCGGTATCAGCCCGAGCAATATCAAGGACTACCTCGCCATTCCGAATATTGTGGCTTGCGGCGGATCTTGGTTTGT
CGAGAAATCTTTGATTCAAGCGAAGAATTGGGATGAAATCGGTAGATTGGTACGAGAAGCCGTAGCACTAACCCATGCTT
AA

Upstream 100 bases:

>100_bases
TAGCCGGTATAGTGATTCAGCACAAAGGCGCAATTATAGATAAATCGGCGACCGCACATTTATTCGCTCAATTTAATGCT
ATGTAGTAAGGAAAAAAATG

Downstream 100 bases:

>100_bases
TTACAAGCAAGCGGTTTGATTTTCTCTGCTTTTTGCAAAAAACGTGCAAAATCAGACCGCTTGAAAATAGCGTACTAAAA
AAGAAAAAGTAATTTGAATC

Product: keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MKYTTEQIIEKLRQLKVVPVIAVEQAEDILPLVKTLAENGLPVAEITFRSAAAEEAIRLVCQHYPDVLIAAGTVLTAEQV
VKAKNAGADFVVTPGFNPTIVKLCQDLDFPITPGVNNPMSIEAALSLGIEAVKFFPAEASGGVKMIKVLLGPYGNLQIMP
TGGISPSNIKDYLAIPNIVACGGSWFVEKSLIQAKNWDEIGRLVREAVALTHA

Sequences:

>Translated_213_residues
MKYTTEQIIEKLRQLKVVPVIAVEQAEDILPLVKTLAENGLPVAEITFRSAAAEEAIRLVCQHYPDVLIAAGTVLTAEQV
VKAKNAGADFVVTPGFNPTIVKLCQDLDFPITPGVNNPMSIEAALSLGIEAVKFFPAEASGGVKMIKVLLGPYGNLQIMP
TGGISPSNIKDYLAIPNIVACGGSWFVEKSLIQAKNWDEIGRLVREAVALTHA
>Mature_213_residues
MKYTTEQIIEKLRQLKVVPVIAVEQAEDILPLVKTLAENGLPVAEITFRSAAAEEAIRLVCQHYPDVLIAAGTVLTAEQV
VKAKNAGADFVVTPGFNPTIVKLCQDLDFPITPGVNNPMSIEAALSLGIEAVKFFPAEASGGVKMIKVLLGPYGNLQIMP
TGGISPSNIKDYLAIPNIVACGGSWFVEKSLIQAKNWDEIGRLVREAVALTHA

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=182, Percent_Identity=37.9120879120879, Blast_Score=154, Evalue=4e-39,
Organism=Escherichia coli, GI48994954, Length=162, Percent_Identity=27.7777777777778, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 22819; Mature: 22819

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00159 ALDOLASE_KDPG_KHG_1 ; PS00160 ALDOLASE_KDPG_KHG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYTTEQIIEKLRQLKVVPVIAVEQAEDILPLVKTLAENGLPVAEITFRSAAAEEAIRLV
CCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHH
CQHYPDVLIAAGTVLTAEQVVKAKNAGADFVVTPGFNPTIVKLCQDLDFPITPGVNNPMS
HHHCCCCEECCCHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHCCCCCCCCCCCCCH
IEAALSLGIEAVKFFPAEASGGVKMIKVLLGPYGNLQIMPTGGISPSNIKDYLAIPNIVA
HHHHHHHCHHHHHEECCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHCCCCEE
CGGSWFVEKSLIQAKNWDEIGRLVREAVALTHA
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKYTTEQIIEKLRQLKVVPVIAVEQAEDILPLVKTLAENGLPVAEITFRSAAAEEAIRLV
CCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHH
CQHYPDVLIAAGTVLTAEQVVKAKNAGADFVVTPGFNPTIVKLCQDLDFPITPGVNNPMS
HHHCCCCEECCCHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHCCCCCCCCCCCCCH
IEAALSLGIEAVKFFPAEASGGVKMIKVLLGPYGNLQIMPTGGISPSNIKDYLAIPNIVA
HHHHHHHCHHHHHEECCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHCCCCEE
CGGSWFVEKSLIQAKNWDEIGRLVREAVALTHA
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]