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The map label for this gene is nrdB [H]

Identifier: 126208467

GI number: 126208467

Start: 1148036

End: 1149166

Strand: Direct

Name: nrdB [H]

Synonym: APL_0993

Alternate gene names: 126208467

Gene position: 1148036-1149166 (Clockwise)

Preceding gene: 126208466

Following gene: 126208468

Centisome position: 50.47

GC content: 40.05

Gene sequence:

>1131_bases
ATGGCATACACCACTTTCTCACAAAATAAAAATGATCAATTAAAAGAACCGATGTTTTTCGGGCAAAACGTGAATGTTGC
TCGTTATGACCAACAAAAATATGAATTATTTGAAAAGTTAATCGAAAAACAGCTTTCATTTTTCTGGCGTCCGGAAGAAG
TTGATGTCTCGCAAGATCGCATCGACTACCAATCATTGCCGGAACACGAAAAACATATTTTTATCAGTAACTTAAAATAT
CAAACACTACTTGATTCCATTCAAGGACGTAGCCCGAACGTGGCATTTTTGCCGTTAGTATCGATTCCGGAGTTGGAAAC
TTGGATCGAAACTTGGACGTTTTCCGAAACGATCCATTCACGTTCTTATACGCACATTATCCGTAATATCGTGAATGATC
CGTCGATTGTGTTTGACGATATCGTGACTAACGAAGAAATTATTAAACGCGCGAAAGATATTTCTTGCTACTACGATGAT
TTAATTCGAGATTCGCAACTTTATACGCTCTACGGAGAAGGTTGCTATACGGTGGACGGCAAGCAATACCAAGTGACGTT
ACGCAACTTAAAACGCCAACTTTATTTATGCTTAATGAGTGTGAACGTACTGGAGGCGATTCGTTTTTACGTATCGTTCG
CTTGTTCTTTTGCGTTTGCCGAGCGTCAGTTAATGGAAGGTAACGCTAAAATTATTAAATTCATTGCACGTGATGAATAT
CTACATTTAACCGGCACACAAAATATGTTGAACATTATCGCATCCGGACAAGACGATCCGGAAATGGCGGAAATTGCCGA
AGAATGTAAGCAAGAAGCTTATGATCTGTTTGTAGCGGCGGCGGAACAAGAAAAAGAATGGGCGGCGTATTTATTTAAAG
ACGGCTCAATGATTGGTTTGAATGAAGATATTTTGGTGAAATATGTCGAATATATTACCAATATTCGGATGCAAGCGGTC
GGATTACCCCGCCCTTTTGCCGCCTCTTCCAATCCGATTCCTTGGATTAACGCTTGGTTGGTGTCTGATAACGTACAAGT
TGCGCCGCAAGAAGTTGAAGTCAGTTCCTATTTAGTCGGGCAAATTAATGCCAAAGTCGATACTAACGATTTCGACAGCT
TTGAACTTTAA

Upstream 100 bases:

>100_bases
TTTAACATTTTTTCCCCACTCCCTCATTTTTCTTCTGTACGAAGAAAAATCTGTCCCTCTCCCACAAGGGGCGAGGGTAA
TTATTTAAAAAGGAGATAAC

Downstream 100 bases:

>100_bases
AGAAAAACAAGCGGTCGCTTTTCCATTATCTTTTGCAAAAAAGAGGGAAAATTCGACCGCTTACTTAAATCGAAATCCAT
AAAAATTCTATTGATTTCTA

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MAYTTFSQNKNDQLKEPMFFGQNVNVARYDQQKYELFEKLIEKQLSFFWRPEEVDVSQDRIDYQSLPEHEKHIFISNLKY
QTLLDSIQGRSPNVAFLPLVSIPELETWIETWTFSETIHSRSYTHIIRNIVNDPSIVFDDIVTNEEIIKRAKDISCYYDD
LIRDSQLYTLYGEGCYTVDGKQYQVTLRNLKRQLYLCLMSVNVLEAIRFYVSFACSFAFAERQLMEGNAKIIKFIARDEY
LHLTGTQNMLNIIASGQDDPEMAEIAEECKQEAYDLFVAAAEQEKEWAAYLFKDGSMIGLNEDILVKYVEYITNIRMQAV
GLPRPFAASSNPIPWINAWLVSDNVQVAPQEVEVSSYLVGQINAKVDTNDFDSFEL

Sequences:

>Translated_376_residues
MAYTTFSQNKNDQLKEPMFFGQNVNVARYDQQKYELFEKLIEKQLSFFWRPEEVDVSQDRIDYQSLPEHEKHIFISNLKY
QTLLDSIQGRSPNVAFLPLVSIPELETWIETWTFSETIHSRSYTHIIRNIVNDPSIVFDDIVTNEEIIKRAKDISCYYDD
LIRDSQLYTLYGEGCYTVDGKQYQVTLRNLKRQLYLCLMSVNVLEAIRFYVSFACSFAFAERQLMEGNAKIIKFIARDEY
LHLTGTQNMLNIIASGQDDPEMAEIAEECKQEAYDLFVAAAEQEKEWAAYLFKDGSMIGLNEDILVKYVEYITNIRMQAV
GLPRPFAASSNPIPWINAWLVSDNVQVAPQEVEVSSYLVGQINAKVDTNDFDSFEL
>Mature_375_residues
AYTTFSQNKNDQLKEPMFFGQNVNVARYDQQKYELFEKLIEKQLSFFWRPEEVDVSQDRIDYQSLPEHEKHIFISNLKYQ
TLLDSIQGRSPNVAFLPLVSIPELETWIETWTFSETIHSRSYTHIIRNIVNDPSIVFDDIVTNEEIIKRAKDISCYYDDL
IRDSQLYTLYGEGCYTVDGKQYQVTLRNLKRQLYLCLMSVNVLEAIRFYVSFACSFAFAERQLMEGNAKIIKFIARDEYL
HLTGTQNMLNIIASGQDDPEMAEIAEECKQEAYDLFVAAAEQEKEWAAYLFKDGSMIGLNEDILVKYVEYITNIRMQAVG
LPRPFAASSNPIPWINAWLVSDNVQVAPQEVEVSSYLVGQINAKVDTNDFDSFEL

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Escherichia coli, GI1788567, Length=376, Percent_Identity=81.9148936170213, Blast_Score=651, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 43607; Mature: 43476

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: PS00368 RIBORED_SMALL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYTTFSQNKNDQLKEPMFFGQNVNVARYDQQKYELFEKLIEKQLSFFWRPEEVDVSQDR
CCEECCCCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHEECCCCCCCCHHH
IDYQSLPEHEKHIFISNLKYQTLLDSIQGRSPNVAFLPLVSIPELETWIETWTFSETIHS
CCHHCCCCCCCEEEEECCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
RSYTHIIRNIVNDPSIVFDDIVTNEEIIKRAKDISCYYDDLIRDSQLYTLYGEGCYTVDG
HHHHHHHHHHCCCCCEEHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEECC
KQYQVTLRNLKRQLYLCLMSVNVLEAIRFYVSFACSFAFAERQLMEGNAKIIKFIARDEY
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEECCCE
LHLTGTQNMLNIIASGQDDPEMAEIAEECKQEAYDLFVAAAEQEKEWAAYLFKDGSMIGL
EEEECCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEECC
NEDILVKYVEYITNIRMQAVGLPRPFAASSNPIPWINAWLVSDNVQVAPQEVEVSSYLVG
CHHHHHHHHHHHHCCHHEEECCCCCCCCCCCCCCEEEEEEEECCCEECCCHHHHHHEEEE
QINAKVDTNDFDSFEL
EEECEECCCCCCCCCC
>Mature Secondary Structure 
AYTTFSQNKNDQLKEPMFFGQNVNVARYDQQKYELFEKLIEKQLSFFWRPEEVDVSQDR
CEECCCCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHEECCCCCCCCHHH
IDYQSLPEHEKHIFISNLKYQTLLDSIQGRSPNVAFLPLVSIPELETWIETWTFSETIHS
CCHHCCCCCCCEEEEECCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
RSYTHIIRNIVNDPSIVFDDIVTNEEIIKRAKDISCYYDDLIRDSQLYTLYGEGCYTVDG
HHHHHHHHHHCCCCCEEHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEECC
KQYQVTLRNLKRQLYLCLMSVNVLEAIRFYVSFACSFAFAERQLMEGNAKIIKFIARDEY
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEECCCE
LHLTGTQNMLNIIASGQDDPEMAEIAEECKQEAYDLFVAAAEQEKEWAAYLFKDGSMIGL
EEEECCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEECC
NEDILVKYVEYITNIRMQAVGLPRPFAASSNPIPWINAWLVSDNVQVAPQEVEVSSYLVG
CHHHHHHHHHHHHCCHHEEECCCCCCCCCCCCCCEEEEEEEECCCEECCCHHHHHHEEEE
QINAKVDTNDFDSFEL
EEECEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]