Click here to switch to the map view.

The map label for this gene is bioF [C]

Identifier: 126208417

GI number: 126208417

Start: 1081651

End: 1082814

Strand: Reverse

Name: bioF [C]

Synonym: APL_0941

Alternate gene names: 126208417

Gene position: 1082814-1081651 (Counterclockwise)

Preceding gene: 126208418

Following gene: 126208416

Centisome position: 47.61

GC content: 42.7

Gene sequence:

>1164_bases
ATGAATGTATTCGAACAAAAGTTAGCGGAATTAACGCAAGCCGGTTTAAAGCGAGCGTTACCGCCGATTCAACACGATGG
GAAGTGGATTATTGCCGATAATCGTCGAATGCTAAATTTTTCTTCAAACGATTATTTGGGGCTAGCTTCAGATCAGCTCT
TACAAAAACAATTTTTGCAAAAAATCAATGAAAATGAACCGCTTGTTACCCTGCTTACCTCATCTTCCTCGCGTCTGCTA
ACCGGTAATTTCCCGATTTACCGAGATTTTGAACAGCTGATTGCCAACCGTTTTCAGCGAGAAGCGGCTTTGTTATTTAA
CAGCGGTTATCATGCCAATATCGGTATTTTGCCGGCGTTATCGGATAAAAATACCCTCATTCTTGCCGATAAGTTAGTAC
ACGCCAGTTTGATTGACGGTATCCGTTTAGCCGGCGTGTCTTTTTATCGTTATCAACATAATAACCTCGAACATTTAACT
CAATTACTTAGCAAATATGCTGCACAGTTTGAGCGAATTATCATTGTGACCGAAAGTATTTTTAGTATGGACGGCGATAT
TGCGCCTTTGGCTGAACTCGTGGCATTAAAAAAGGCCTTTGCCAGCCAGTGTCACATTATGCTGTATGTGGATGAAGCGC
ACGCAATCGGGGCATACGGAGAACAGGGATTAGGCATGGCGGAAGTATTAGGCTGTATCGATGAGATCGATTTTTTAGTC
GGTACGTTCGGTAAAGCATTGGCTTCGATGGGGGCTTATTTAGTGTGCGATCAAGTGATTAAGGAATACTTAATCAACAC
GATGCGTCCGCTGATTTTTTCAACGGCATTACCGCCACTAAATGTCGCGTGGACGGCATTTTTATTTGAGCGGTTACCCG
AATTTCAACCTAAACGACAGCATTTAGCGCATCTCAGTGAGAAGCTAAAACAAGCGGTACAGCAGAAAAATAGTTATCCG
CTACAAACGCAAAGTTGCATTATTCCGTTTATCGTGGGAGAAAACGAACAAGCGGTACAAAAAGCCAAAACATTGCAACA
ACAAGGTTTTTATTGCTTGCCGATTCGCCCGCCGACAGTACCGAAAGGTACGGCTCGTATCCGACTATCCTTAACCGCAG
ATGTAACCACAGAAGAATTAGATCGCTTAATTGCTTGTTTATAA

Upstream 100 bases:

>100_bases
TTATTAGTCAGGCGGAATTAACCCAACTCACTAGCGCATTATTGCAAGTATTAAAAATAGCTTATAACGAATAAAACGTA
ATACATTTTACGGAGGTTTT

Downstream 100 bases:

>100_bases
GGAGACAAAGTGAAAACAAAATTAATTTCAACTCAACAAGCCAATTTGATTATTTATTTTGCCGGTTGGGGCACACCGGC
ATCAGTCGTCTCACATTTAG

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS/AKB ligase; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; Alpha-oxoamine synthase; L-alanine--pimeloyl-CoA ligase [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MNVFEQKLAELTQAGLKRALPPIQHDGKWIIADNRRMLNFSSNDYLGLASDQLLQKQFLQKINENEPLVTLLTSSSSRLL
TGNFPIYRDFEQLIANRFQREAALLFNSGYHANIGILPALSDKNTLILADKLVHASLIDGIRLAGVSFYRYQHNNLEHLT
QLLSKYAAQFERIIIVTESIFSMDGDIAPLAELVALKKAFASQCHIMLYVDEAHAIGAYGEQGLGMAEVLGCIDEIDFLV
GTFGKALASMGAYLVCDQVIKEYLINTMRPLIFSTALPPLNVAWTAFLFERLPEFQPKRQHLAHLSEKLKQAVQQKNSYP
LQTQSCIIPFIVGENEQAVQKAKTLQQQGFYCLPIRPPTVPKGTARIRLSLTADVTTEELDRLIACL

Sequences:

>Translated_387_residues
MNVFEQKLAELTQAGLKRALPPIQHDGKWIIADNRRMLNFSSNDYLGLASDQLLQKQFLQKINENEPLVTLLTSSSSRLL
TGNFPIYRDFEQLIANRFQREAALLFNSGYHANIGILPALSDKNTLILADKLVHASLIDGIRLAGVSFYRYQHNNLEHLT
QLLSKYAAQFERIIIVTESIFSMDGDIAPLAELVALKKAFASQCHIMLYVDEAHAIGAYGEQGLGMAEVLGCIDEIDFLV
GTFGKALASMGAYLVCDQVIKEYLINTMRPLIFSTALPPLNVAWTAFLFERLPEFQPKRQHLAHLSEKLKQAVQQKNSYP
LQTQSCIIPFIVGENEQAVQKAKTLQQQGFYCLPIRPPTVPKGTARIRLSLTADVTTEELDRLIACL
>Mature_387_residues
MNVFEQKLAELTQAGLKRALPPIQHDGKWIIADNRRMLNFSSNDYLGLASDQLLQKQFLQKINENEPLVTLLTSSSSRLL
TGNFPIYRDFEQLIANRFQREAALLFNSGYHANIGILPALSDKNTLILADKLVHASLIDGIRLAGVSFYRYQHNNLEHLT
QLLSKYAAQFERIIIVTESIFSMDGDIAPLAELVALKKAFASQCHIMLYVDEAHAIGAYGEQGLGMAEVLGCIDEIDFLV
GTFGKALASMGAYLVCDQVIKEYLINTMRPLIFSTALPPLNVAWTAFLFERLPEFQPKRQHLAHLSEKLKQAVQQKNSYP
LQTQSCIIPFIVGENEQAVQKAKTLQQQGFYCLPIRPPTVPKGTARIRLSLTADVTTEELDRLIACL

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A and/or converts 2-amino-3-ketobutyrate to glycine and acetyl-CoA [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI7657118, Length=350, Percent_Identity=33.7142857142857, Blast_Score=185, Evalue=6e-47,
Organism=Homo sapiens, GI284448556, Length=350, Percent_Identity=33.7142857142857, Blast_Score=185, Evalue=7e-47,
Organism=Homo sapiens, GI83977444, Length=377, Percent_Identity=29.4429708222812, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI83977440, Length=377, Percent_Identity=29.4429708222812, Blast_Score=148, Evalue=7e-36,
Organism=Homo sapiens, GI83977442, Length=377, Percent_Identity=29.4429708222812, Blast_Score=148, Evalue=7e-36,
Organism=Homo sapiens, GI119220554, Length=369, Percent_Identity=27.3712737127371, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI4758668, Length=268, Percent_Identity=32.089552238806, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI4502025, Length=370, Percent_Identity=27.5675675675676, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI40316939, Length=370, Percent_Identity=27.5675675675676, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI5454084, Length=384, Percent_Identity=22.9166666666667, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI1786993, Length=369, Percent_Identity=36.5853658536585, Blast_Score=221, Evalue=6e-59,
Organism=Escherichia coli, GI1790046, Length=356, Percent_Identity=30.8988764044944, Blast_Score=173, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI71994529, Length=364, Percent_Identity=31.3186813186813, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17560914, Length=404, Percent_Identity=26.7326732673267, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17560912, Length=404, Percent_Identity=26.7326732673267, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI32566772, Length=333, Percent_Identity=29.7297297297297, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71982625, Length=279, Percent_Identity=24.7311827956989, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI71982617, Length=279, Percent_Identity=24.7311827956989, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320267, Length=366, Percent_Identity=28.4153005464481, Blast_Score=142, Evalue=9e-35,
Organism=Saccharomyces cerevisiae, GI6320438, Length=375, Percent_Identity=27.7333333333333, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6323954, Length=247, Percent_Identity=27.9352226720648, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24662918, Length=388, Percent_Identity=30.9278350515464, Blast_Score=184, Evalue=8e-47,
Organism=Drosophila melanogaster, GI17137420, Length=351, Percent_Identity=30.1994301994302, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI17136286, Length=364, Percent_Identity=28.8461538461538, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI24653280, Length=282, Percent_Identity=27.6595744680851, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24653276, Length=282, Percent_Identity=27.6595744680851, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24653278, Length=282, Percent_Identity=27.6595744680851, Blast_Score=106, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.29; =2.3.1.47 [H]

Molecular weight: Translated: 43350; Mature: 43350

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVFEQKLAELTQAGLKRALPPIQHDGKWIIADNRRMLNFSSNDYLGLASDQLLQKQFLQ
CCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHH
KINENEPLVTLLTSSSSRLLTGNFPIYRDFEQLIANRFQREAALLFNSGYHANIGILPAL
HHCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEC
SDKNTLILADKLVHASLIDGIRLAGVSFYRYQHNNLEHLTQLLSKYAAQFERIIIVTESI
CCCCEEEEHHHHHHHHHHCCHHHHCEEEEEECCCCHHHHHHHHHHHHHHHHEEEEEEHHH
FSMDGDIAPLAELVALKKAFASQCHIMLYVDEAHAIGAYGEQGLGMAEVLGCIDEIDFLV
HCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHH
GTFGKALASMGAYLVCDQVIKEYLINTMRPLIFSTALPPLNVAWTAFLFERLPEFQPKRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHH
HLAHLSEKLKQAVQQKNSYPLQTQSCIIPFIVGENEQAVQKAKTLQQQGFYCLPIRPPTV
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCEEEECCCCCC
PKGTARIRLSLTADVTTEELDRLIACL
CCCCEEEEEEEEECCCHHHHHHHHHCC
>Mature Secondary Structure
MNVFEQKLAELTQAGLKRALPPIQHDGKWIIADNRRMLNFSSNDYLGLASDQLLQKQFLQ
CCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHH
KINENEPLVTLLTSSSSRLLTGNFPIYRDFEQLIANRFQREAALLFNSGYHANIGILPAL
HHCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEC
SDKNTLILADKLVHASLIDGIRLAGVSFYRYQHNNLEHLTQLLSKYAAQFERIIIVTESI
CCCCEEEEHHHHHHHHHHCCHHHHCEEEEEECCCCHHHHHHHHHHHHHHHHEEEEEEHHH
FSMDGDIAPLAELVALKKAFASQCHIMLYVDEAHAIGAYGEQGLGMAEVLGCIDEIDFLV
HCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHH
GTFGKALASMGAYLVCDQVIKEYLINTMRPLIFSTALPPLNVAWTAFLFERLPEFQPKRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHH
HLAHLSEKLKQAVQQKNSYPLQTQSCIIPFIVGENEQAVQKAKTLQQQGFYCLPIRPPTV
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCEEEECCCCCC
PKGTARIRLSLTADVTTEELDRLIACL
CCCCEEEEEEEEECCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA