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The map label for this gene is iscS [H]

Identifier: 126208407

GI number: 126208407

Start: 1071830

End: 1073056

Strand: Reverse

Name: iscS [H]

Synonym: APL_0931

Alternate gene names: 126208407

Gene position: 1073056-1071830 (Counterclockwise)

Preceding gene: 126208408

Following gene: 126208406

Centisome position: 47.18

GC content: 43.36

Gene sequence:

>1227_bases
ATGTCAGTTAAATTACCTATCTATTTAGATTATGCAGCAACTACGCCGATGGACGAGCGTGTTGCGCAAAAAATGATGCA
ATATATGACCAAAGACGGCATCTTTGGTAACCCGGCTTCACGTTCACATAAATTCGGTTGGGAAGCGGAAGAAGCGGTAG
ATGTTGCACGTAACCATATTGCGGATTTAATCGGCGCGGACAGCCGTGAAATCGTATTTACTTCAGGTGCGACGGAGTCG
GATAATCTTGCGATCAAAGGTGCGGCGCATTTTTACCAAACTAAAGGTAAACATATCATCACGGTTAAAACCGAACACAA
AGCGGTATTGGATACTTGCCGTCAGTTAGAGCGTGAAGGTTTTGAAGTAACTTACTTAGATCCGGAAGAAGACGGTATTT
TAGATTTAGCGAAATTAGAAGCGGCGATTCGTCCGGATACCATTTTAGTTTCCGTAATGCACGTAAATAACGAAATGGGC
GTTATCCAAGACATCAAAGCAATCGGTGAGATTTGCCGTGCGAAGAAAATTATTTTCCATGTGGATGCAACGCAATCGGT
CGGTAAAGTACCGGTAAACGTACAAGAATTAAAAGTGGATTTAATGTCTTTCTCAAGCCACAAATTATACGGACCGAAAG
GCATCGGCGGCTTATATGTATGCCGTAAACCTCGTGTTCGCATCGAAGCGATTATTCACGGTGGCGGTCATGAGCGCGGT
ATGCGTTCGGGCACATTACCTGTACACCAAATCGTAGGTATGGGTGAAGCGTATCGTATCGCTAAAGAAGAAATGGCAAC
CGAAATGCCTCGCTTACGTGCGTTACGCGACCGTTTATACAACGGCTTTAAAGATATTGAAGAAGTTTATGTAAACGGTT
CGATGGAAGAAGGTAAACGTGTAGATTCAAACTTAAATATCAGCTTCAACTTCGTTGAAGGCGAATCATTAATGATGGCG
TTACGTGATATTGCGGTTTCGTCAGGTTCAGCGTGTACTTCGGCAAGTTTAGAGCCTTCATACGTATTACGCGCAATCGG
TCGTGACGATGAATTGGCACACAGCTCGATTCGTTTCACTTTAGGTCGTTGGACAACCGAAGAAGAAATCGACTACACCA
TCGAACTTGTGAAAAAAGCAATCGTAAAACTTCGTGAACTTTCTCCGCTTTGGGAAATGTTTAAAGAAGGTGTGGATCTA
TCAAAAATCGAGTGGAATCACCACTAA

Upstream 100 bases:

>100_bases
CGATTGTGAAAAAGAACATTGCCACGATCATTCACACAAACATTAATTGCAACAATTTCAATAAAAACGACCGCTTATTT
AAAGCGAGTTAGGAGTAACA

Downstream 100 bases:

>100_bases
TCAGTAGAAATATTGCAATGCATTTGCGTTGTTGAACCGCCTTGCCGTACTGATTGTACTGTCTGCGGCGGTTCGCCTAG
CAACTGCAAACAATATTTTA

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MSVKLPIYLDYAATTPMDERVAQKMMQYMTKDGIFGNPASRSHKFGWEAEEAVDVARNHIADLIGADSREIVFTSGATES
DNLAIKGAAHFYQTKGKHIITVKTEHKAVLDTCRQLEREGFEVTYLDPEEDGILDLAKLEAAIRPDTILVSVMHVNNEMG
VIQDIKAIGEICRAKKIIFHVDATQSVGKVPVNVQELKVDLMSFSSHKLYGPKGIGGLYVCRKPRVRIEAIIHGGGHERG
MRSGTLPVHQIVGMGEAYRIAKEEMATEMPRLRALRDRLYNGFKDIEEVYVNGSMEEGKRVDSNLNISFNFVEGESLMMA
LRDIAVSSGSACTSASLEPSYVLRAIGRDDELAHSSIRFTLGRWTTEEEIDYTIELVKKAIVKLRELSPLWEMFKEGVDL
SKIEWNHH

Sequences:

>Translated_408_residues
MSVKLPIYLDYAATTPMDERVAQKMMQYMTKDGIFGNPASRSHKFGWEAEEAVDVARNHIADLIGADSREIVFTSGATES
DNLAIKGAAHFYQTKGKHIITVKTEHKAVLDTCRQLEREGFEVTYLDPEEDGILDLAKLEAAIRPDTILVSVMHVNNEMG
VIQDIKAIGEICRAKKIIFHVDATQSVGKVPVNVQELKVDLMSFSSHKLYGPKGIGGLYVCRKPRVRIEAIIHGGGHERG
MRSGTLPVHQIVGMGEAYRIAKEEMATEMPRLRALRDRLYNGFKDIEEVYVNGSMEEGKRVDSNLNISFNFVEGESLMMA
LRDIAVSSGSACTSASLEPSYVLRAIGRDDELAHSSIRFTLGRWTTEEEIDYTIELVKKAIVKLRELSPLWEMFKEGVDL
SKIEWNHH
>Mature_407_residues
SVKLPIYLDYAATTPMDERVAQKMMQYMTKDGIFGNPASRSHKFGWEAEEAVDVARNHIADLIGADSREIVFTSGATESD
NLAIKGAAHFYQTKGKHIITVKTEHKAVLDTCRQLEREGFEVTYLDPEEDGILDLAKLEAAIRPDTILVSVMHVNNEMGV
IQDIKAIGEICRAKKIIFHVDATQSVGKVPVNVQELKVDLMSFSSHKLYGPKGIGGLYVCRKPRVRIEAIIHGGGHERGM
RSGTLPVHQIVGMGEAYRIAKEEMATEMPRLRALRDRLYNGFKDIEEVYVNGSMEEGKRVDSNLNISFNFVEGESLMMAL
RDIAVSSGSACTSASLEPSYVLRAIGRDDELAHSSIRFTLGRWTTEEEIDYTIELVKKAIVKLRELSPLWEMFKEGVDLS
KIEWNHH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=404, Percent_Identity=57.4257425742574, Blast_Score=500, Evalue=1e-141,
Organism=Homo sapiens, GI156713448, Length=417, Percent_Identity=30.4556354916067, Blast_Score=181, Evalue=9e-46,
Organism=Escherichia coli, GI48994898, Length=406, Percent_Identity=80.0492610837438, Blast_Score=691, Evalue=0.0,
Organism=Escherichia coli, GI1787970, Length=224, Percent_Identity=33.0357142857143, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789175, Length=215, Percent_Identity=29.7674418604651, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI25143064, Length=405, Percent_Identity=57.7777777777778, Blast_Score=483, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI17533177, Length=331, Percent_Identity=31.7220543806647, Blast_Score=139, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6319831, Length=403, Percent_Identity=56.3275434243176, Blast_Score=482, Evalue=1e-137,
Organism=Drosophila melanogaster, GI20129463, Length=404, Percent_Identity=55.6930693069307, Blast_Score=473, Evalue=1e-134,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 45700; Mature: 45569

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVKLPIYLDYAATTPMDERVAQKMMQYMTKDGIFGNPASRSHKFGWEAEEAVDVARNHI
CCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ADLIGADSREIVFTSGATESDNLAIKGAAHFYQTKGKHIITVKTEHKAVLDTCRQLEREG
HHHHCCCCCEEEEECCCCCCCCEEEEEHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCC
FEVTYLDPEEDGILDLAKLEAAIRPDTILVSVMHVNNEMGVIQDIKAIGEICRAKKIIFH
CEEEEECCCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCEEEEE
VDATQSVGKVPVNVQELKVDLMSFSSHKLYGPKGIGGLYVCRKPRVRIEAIIHGGGHERG
EECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCEEEEEEEECCCCCCC
MRSGTLPVHQIVGMGEAYRIAKEEMATEMPRLRALRDRLYNGFKDIEEVYVNGSMEEGKR
CCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCC
VDSNLNISFNFVEGESLMMALRDIAVSSGSACTSASLEPSYVLRAIGRDDELAHSSIRFT
CCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHEEEE
LGRWTTEEEIDYTIELVKKAIVKLRELSPLWEMFKEGVDLSKIEWNHH
ECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEECCCC
>Mature Secondary Structure 
SVKLPIYLDYAATTPMDERVAQKMMQYMTKDGIFGNPASRSHKFGWEAEEAVDVARNHI
CEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ADLIGADSREIVFTSGATESDNLAIKGAAHFYQTKGKHIITVKTEHKAVLDTCRQLEREG
HHHHCCCCCEEEEECCCCCCCCEEEEEHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCC
FEVTYLDPEEDGILDLAKLEAAIRPDTILVSVMHVNNEMGVIQDIKAIGEICRAKKIIFH
CEEEEECCCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCEEEEE
VDATQSVGKVPVNVQELKVDLMSFSSHKLYGPKGIGGLYVCRKPRVRIEAIIHGGGHERG
EECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCEEEEEEEECCCCCCC
MRSGTLPVHQIVGMGEAYRIAKEEMATEMPRLRALRDRLYNGFKDIEEVYVNGSMEEGKR
CCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCC
VDSNLNISFNFVEGESLMMALRDIAVSSGSACTSASLEPSYVLRAIGRDDELAHSSIRFT
CCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHEEEE
LGRWTTEEEIDYTIELVKKAIVKLRELSPLWEMFKEGVDLSKIEWNHH
ECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA