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The map label for this gene is cca

Identifier: 126208391

GI number: 126208391

Start: 1056460

End: 1057695

Strand: Direct

Name: cca

Synonym: APL_0915

Alternate gene names: 126208391

Gene position: 1056460-1057695 (Clockwise)

Preceding gene: 126208390

Following gene: 126208392

Centisome position: 46.45

GC content: 43.04

Gene sequence:

>1236_bases
ATGCAAATTTATCTCGTTGGCGGTGCAGTCCGTGATCAACTATTAAACTTACCGGTAAAAGATCGGGATTATCTGGTGGT
CGGCGCAACGCCCGAACAATTATTAGCACAAGGTTATCAGCAAGTCGGCAATGACTTTCCCGTTTTTTTACATCCAAAAA
CCAAAGAAGAATATGCGCTAGCCCGCCAAGAACGTAAAAACGGGGTCGGCTATAATGGTTTTCTCTGCGACTTTTCACCG
GATGTCACCCTCGAACAAGATTTAATTCGCCGAGATTTAACTATTAATGCGATTGCCCAAGATGCAAGCGGTCAAATTTT
TGACCCTTACGGCGGTAAACAAGATTTAGCAAATCGCCTGCTTCGTCATATTTCGCCGGCATTTAGTGAAGATCCTCTGC
GAGTTTTAAGAGTAGCTCGCTTTGCCGCCCGTTTCCATTCGCTTGGCTTTAAGATCGCCCCTGAAACGGTAAAATTAATG
CAAGAAATGACCGCTTGTGGTGAATTGGCTCACTTAACTGCGGAGCGTGTTTGGTTAGAAACCCAAAAAGCGTTTGCAAC
GGATAATCCGCAAATCTATTTTGAAGTATTACGAGAAATCGGCGCATTAGCCGTATTATTTCCGGAATTCGATCGCTTAT
TCGGTGTACCTCAACCCGAACAACATCACCCTGAAATTGATAGTGGGGTTCATACCTTACTGGTAATTGAGCAAGCCAAA
AGACTTGCAAAAAATGCAGAAAATCCGACCGCTTTACTCTGGGCTGCACTTTGCCACGATTTAGGCAAAGGGTTAACCGA
AAAAGACATTCTCCCACATCATTACGGGCACGAAGTAAAAGGTGTCAAACCGGCTCGAGAACTTTCAAATCGCCTGAAAG
TGTCAACAGACGTAAAAGATTTCGCCATTTTGGTCACTGAATACCACACCCACTGCCATAAAATGGCGGAGCTGCGTCCG
GAAACCGTATTAAAAGTATTTAATGCACTTGATATATGGCGTAAACCGCAACGCTTCTGTGATTTTTTACTCGCTTGTGA
AGCGGATGCTCGTGGCAGATTAGGTTTTGAAAATAGGGAATATCCACAAGCTGAACTTGCTAAATGGTATTTTAAAGTAG
CAGCCCAAGTAGATGTACAAGCAGTGATTCAAGACGGTTTTGAAAAAAAAGCAATCAGGGAAGAGTTGAATAAGCGGCGA
ATTACAGCAATAAAAATAATAAAACCCAATGTATAA

Upstream 100 bases:

>100_bases
CAAAATCCATTAGAAATTTGACCGCTTGCACTCAACACAAGCGGTCAAATTTTTTATCATTCTGCAAAATGGAATCCTCT
TTCATAAAAGTAACAAAACT

Downstream 100 bases:

>100_bases
AACAAGGAGCCTTCTGATCTGCCCCCCAAAAGTTGGACTAAACCTCCAACGGATTAAGGTGCAGATTTTTTATGACTAAA
TATAACTCGCTATTCAAACA

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MQIYLVGGAVRDQLLNLPVKDRDYLVVGATPEQLLAQGYQQVGNDFPVFLHPKTKEEYALARQERKNGVGYNGFLCDFSP
DVTLEQDLIRRDLTINAIAQDASGQIFDPYGGKQDLANRLLRHISPAFSEDPLRVLRVARFAARFHSLGFKIAPETVKLM
QEMTACGELAHLTAERVWLETQKAFATDNPQIYFEVLREIGALAVLFPEFDRLFGVPQPEQHHPEIDSGVHTLLVIEQAK
RLAKNAENPTALLWAALCHDLGKGLTEKDILPHHYGHEVKGVKPARELSNRLKVSTDVKDFAILVTEYHTHCHKMAELRP
ETVLKVFNALDIWRKPQRFCDFLLACEADARGRLGFENREYPQAELAKWYFKVAAQVDVQAVIQDGFEKKAIREELNKRR
ITAIKIIKPNV

Sequences:

>Translated_411_residues
MQIYLVGGAVRDQLLNLPVKDRDYLVVGATPEQLLAQGYQQVGNDFPVFLHPKTKEEYALARQERKNGVGYNGFLCDFSP
DVTLEQDLIRRDLTINAIAQDASGQIFDPYGGKQDLANRLLRHISPAFSEDPLRVLRVARFAARFHSLGFKIAPETVKLM
QEMTACGELAHLTAERVWLETQKAFATDNPQIYFEVLREIGALAVLFPEFDRLFGVPQPEQHHPEIDSGVHTLLVIEQAK
RLAKNAENPTALLWAALCHDLGKGLTEKDILPHHYGHEVKGVKPARELSNRLKVSTDVKDFAILVTEYHTHCHKMAELRP
ETVLKVFNALDIWRKPQRFCDFLLACEADARGRLGFENREYPQAELAKWYFKVAAQVDVQAVIQDGFEKKAIREELNKRR
ITAIKIIKPNV
>Mature_411_residues
MQIYLVGGAVRDQLLNLPVKDRDYLVVGATPEQLLAQGYQQVGNDFPVFLHPKTKEEYALARQERKNGVGYNGFLCDFSP
DVTLEQDLIRRDLTINAIAQDASGQIFDPYGGKQDLANRLLRHISPAFSEDPLRVLRVARFAARFHSLGFKIAPETVKLM
QEMTACGELAHLTAERVWLETQKAFATDNPQIYFEVLREIGALAVLFPEFDRLFGVPQPEQHHPEIDSGVHTLLVIEQAK
RLAKNAENPTALLWAALCHDLGKGLTEKDILPHHYGHEVKGVKPARELSNRLKVSTDVKDFAILVTEYHTHCHKMAELRP
ETVLKVFNALDIWRKPQRFCDFLLACEADARGRLGFENREYPQAELAKWYFKVAAQVDVQAVIQDGFEKKAIREELNKRR
ITAIKIIKPNV

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily

Homologues:

Organism=Homo sapiens, GI155030240, Length=199, Percent_Identity=30.1507537688442, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1789436, Length=408, Percent_Identity=58.578431372549, Blast_Score=494, Evalue=1e-141,
Organism=Escherichia coli, GI87081691, Length=230, Percent_Identity=32.1739130434783, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_ACTP2 (A3N0S5)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053616.1
- ProteinModelPortal:   A3N0S5
- SMR:   A3N0S5
- STRING:   A3N0S5
- GeneID:   4850166
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0915
- eggNOG:   COG0617
- HOGENOM:   HBG635063
- OMA:   RWACLLH
- PhylomeDB:   A3N0S5
- ProtClustDB:   PRK10885
- BioCyc:   APLE416269:APL_0915-MONOMER
- HAMAP:   MF_01261
- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646
- PIRSF:   PIRSF000813
- SMART:   SM00471

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 46680; Mature: 46680

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: NA

Important sites: BINDING 8-8 BINDING 11-11 BINDING 91-91 BINDING 137-137 BINDING 140-140

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIYLVGGAVRDQLLNLPVKDRDYLVVGATPEQLLAQGYQQVGNDFPVFLHPKTKEEYAL
CEEEEECHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCEEECCCCHHHHHH
ARQERKNGVGYNGFLCDFSPDVTLEQDLIRRDLTINAIAQDASGQIFDPYGGKQDLANRL
HHHHHHCCCCCCCEEECCCCCCCHHHHHHHHCCHHHHHHHCCCCCEECCCCCHHHHHHHH
LRHISPAFSEDPLRVLRVARFAARFHSLGFKIAPETVKLMQEMTACGELAHLTAERVWLE
HHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
TQKAFATDNPQIYFEVLREIGALAVLFPEFDRLFGVPQPEQHHPEIDSGVHTLLVIEQAK
HHHHHCCCCCHHHHHHHHHHCHHEEHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHH
RLAKNAENPTALLWAALCHDLGKGLTEKDILPHHYGHEVKGVKPARELSNRLKVSTDVKD
HHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCEECCCHHH
FAILVTEYHTHCHKMAELRPETVLKVFNALDIWRKPQRFCDFLLACEADARGRLGFENRE
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCC
YPQAELAKWYFKVAAQVDVQAVIQDGFEKKAIREELNKRRITAIKIIKPNV
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCEEEEEEECCCC
>Mature Secondary Structure
MQIYLVGGAVRDQLLNLPVKDRDYLVVGATPEQLLAQGYQQVGNDFPVFLHPKTKEEYAL
CEEEEECHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCEEECCCCHHHHHH
ARQERKNGVGYNGFLCDFSPDVTLEQDLIRRDLTINAIAQDASGQIFDPYGGKQDLANRL
HHHHHHCCCCCCCEEECCCCCCCHHHHHHHHCCHHHHHHHCCCCCEECCCCCHHHHHHHH
LRHISPAFSEDPLRVLRVARFAARFHSLGFKIAPETVKLMQEMTACGELAHLTAERVWLE
HHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
TQKAFATDNPQIYFEVLREIGALAVLFPEFDRLFGVPQPEQHHPEIDSGVHTLLVIEQAK
HHHHHCCCCCHHHHHHHHHHCHHEEHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHH
RLAKNAENPTALLWAALCHDLGKGLTEKDILPHHYGHEVKGVKPARELSNRLKVSTDVKD
HHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCEECCCHHH
FAILVTEYHTHCHKMAELRPETVLKVFNALDIWRKPQRFCDFLLACEADARGRLGFENRE
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCC
YPQAELAKWYFKVAAQVDVQAVIQDGFEKKAIREELNKRRITAIKIIKPNV
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA