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The map label for this gene is cysS

Identifier: 126208389

GI number: 126208389

Start: 1054323

End: 1055702

Strand: Reverse

Name: cysS

Synonym: APL_0913

Alternate gene names: 126208389

Gene position: 1055702-1054323 (Counterclockwise)

Preceding gene: 126208395

Following gene: 126208388

Centisome position: 46.42

GC content: 42.83

Gene sequence:

>1380_bases
ATGCTCAAAATTTATAACACTTTAAAACGTGAAAAAGAAGAATTTAAACCGATTAACCCGAACCAAGTTGGTATGTATGT
CTGCGGTGTTACGGTTTACGATCTCTGTCACTTTGGGCACGGACGTACTTTTGTTTCATTTGACGTGATTGCACGTTATT
TACGTTATTTAGGCTATAACTTGCGTTATGTGCGTAACATTACCGATGTGGACGATAAAATCATCAAACGTGCATTAGAA
AACAACGAAACTTGCGATCAATTAGTCGATCGTATGATTGCCGAAATGCATAAAGATTTTGATGATCTGAATATTCTTCG
CCCGGATGTTGAGCCTCGTGCAACTAAACATATTCCGGAAATTGTAGCGATGGTGGAAAAGCTGATCGCAAATGGCCACG
CTTACGTAGCGGCGGACGGCGATGTAATGTTTGATGTGGAAAGCTTCAAAAAATACGGTGCGTTATCTCGACAAAATTTA
GAACAATTACAAGCGGGCGCACGTGTTGAAATTAAATCGGTGAAGAAAAATCCGATGGATTTCGTGTTATGGAAAATGTC
GAAAGAAGGCGAGCCGAGTTGGCAAAGTCCGTGGGGCAACGGTCGCCCGGGTTGGCATATCGAATGTTCGGCAATGAACA
GCAAAGAGTTAGGCGAACATTTTGATATTCACGGCGGTGGCTCGGACTTAATGTTCCCTCACCACGAAAACGAAATTGCG
CAATCTTGCTGCGCACATGGCGGTGATTATGTGAATTACTGGTTACACACCGGTATGTTAACGATTGATGATGAGAAAAT
GTCAAAATCGCTCGGCAATTTCTTCACGATTCGCACCATGTTAGAAAAATATGAATCGGAAACTTTACGTTATTTCTTCT
TAACCGCGCACTATCGTAGCTTACTAAATTACAGCCTAGATAATTTAGATTTAGCACGCTCGGCGTTAGAACGTTTATAC
ACTTCATTACGCGGTTGCGATTTATCGGTGGAAGTTGCCGGTGGCGAACAATACGTCGAAGCGTTTAAAGCTGCAATGGA
TGATGATTTCAATACACCGGGCGCATTAGCGGTGTTATTTGAAATCGCACGTGAAGTGAATAAACTCAAAACCGAAGATA
TGGCGAAAGCGAACGGTTTAGCGGTGCGTTTAAAAGAATTAGCCGGCGTATTAGGTTTACTCTATCAAGATCCGGAAGCG
TTTTTACAAGGCGATGCGGATAATGATGAAGTTGCGGAAATCGAAGCGTTAATCAAACAACGCAACGAAGCAAAAGCAGC
GAAAAACTGGGCGGTTGCCGATGAAGTGCGTGATAAACTAAAAGCAATGAATATCGTGCTGGAAGACACGCCAAACGGCA
CAACGTGGCGTAAAGCATAA

Upstream 100 bases:

>100_bases
ATTAAAAAGTTTACGGGAAATTGCAAAAAAATCTCAAAATTAGACCGCTTATTCGCTATACTTCTCAACATTGCGATAAC
AAATCAGTACAGAGGATACT

Downstream 100 bases:

>100_bases
GCCTTAGCGATAAAATAGCAAGCGGTTGAATTTGTAAAAAATCTTGCAAATCCAACCGCTTTTTTCGTTCTTAGAATGAT
TAGTTGCTTTTTTATTCTTT

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MLKIYNTLKREKEEFKPINPNQVGMYVCGVTVYDLCHFGHGRTFVSFDVIARYLRYLGYNLRYVRNITDVDDKIIKRALE
NNETCDQLVDRMIAEMHKDFDDLNILRPDVEPRATKHIPEIVAMVEKLIANGHAYVAADGDVMFDVESFKKYGALSRQNL
EQLQAGARVEIKSVKKNPMDFVLWKMSKEGEPSWQSPWGNGRPGWHIECSAMNSKELGEHFDIHGGGSDLMFPHHENEIA
QSCCAHGGDYVNYWLHTGMLTIDDEKMSKSLGNFFTIRTMLEKYESETLRYFFLTAHYRSLLNYSLDNLDLARSALERLY
TSLRGCDLSVEVAGGEQYVEAFKAAMDDDFNTPGALAVLFEIAREVNKLKTEDMAKANGLAVRLKELAGVLGLLYQDPEA
FLQGDADNDEVAEIEALIKQRNEAKAAKNWAVADEVRDKLKAMNIVLEDTPNGTTWRKA

Sequences:

>Translated_459_residues
MLKIYNTLKREKEEFKPINPNQVGMYVCGVTVYDLCHFGHGRTFVSFDVIARYLRYLGYNLRYVRNITDVDDKIIKRALE
NNETCDQLVDRMIAEMHKDFDDLNILRPDVEPRATKHIPEIVAMVEKLIANGHAYVAADGDVMFDVESFKKYGALSRQNL
EQLQAGARVEIKSVKKNPMDFVLWKMSKEGEPSWQSPWGNGRPGWHIECSAMNSKELGEHFDIHGGGSDLMFPHHENEIA
QSCCAHGGDYVNYWLHTGMLTIDDEKMSKSLGNFFTIRTMLEKYESETLRYFFLTAHYRSLLNYSLDNLDLARSALERLY
TSLRGCDLSVEVAGGEQYVEAFKAAMDDDFNTPGALAVLFEIAREVNKLKTEDMAKANGLAVRLKELAGVLGLLYQDPEA
FLQGDADNDEVAEIEALIKQRNEAKAAKNWAVADEVRDKLKAMNIVLEDTPNGTTWRKA
>Mature_459_residues
MLKIYNTLKREKEEFKPINPNQVGMYVCGVTVYDLCHFGHGRTFVSFDVIARYLRYLGYNLRYVRNITDVDDKIIKRALE
NNETCDQLVDRMIAEMHKDFDDLNILRPDVEPRATKHIPEIVAMVEKLIANGHAYVAADGDVMFDVESFKKYGALSRQNL
EQLQAGARVEIKSVKKNPMDFVLWKMSKEGEPSWQSPWGNGRPGWHIECSAMNSKELGEHFDIHGGGSDLMFPHHENEIA
QSCCAHGGDYVNYWLHTGMLTIDDEKMSKSLGNFFTIRTMLEKYESETLRYFFLTAHYRSLLNYSLDNLDLARSALERLY
TSLRGCDLSVEVAGGEQYVEAFKAAMDDDFNTPGALAVLFEIAREVNKLKTEDMAKANGLAVRLKELAGVLGLLYQDPEA
FLQGDADNDEVAEIEALIKQRNEAKAAKNWAVADEVRDKLKAMNIVLEDTPNGTTWRKA

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=384, Percent_Identity=39.5833333333333, Blast_Score=251, Evalue=1e-66,
Organism=Homo sapiens, GI62240992, Length=260, Percent_Identity=43.4615384615385, Blast_Score=206, Evalue=3e-53,
Organism=Homo sapiens, GI21269877, Length=260, Percent_Identity=43.4615384615385, Blast_Score=206, Evalue=3e-53,
Organism=Homo sapiens, GI303304987, Length=260, Percent_Identity=43.4615384615385, Blast_Score=206, Evalue=3e-53,
Organism=Homo sapiens, GI10835051, Length=260, Percent_Identity=43.4615384615385, Blast_Score=206, Evalue=4e-53,
Organism=Escherichia coli, GI1786737, Length=460, Percent_Identity=71.0869565217391, Blast_Score=679, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17509795, Length=248, Percent_Identity=45.1612903225806, Blast_Score=203, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17509797, Length=248, Percent_Identity=45.1612903225806, Blast_Score=203, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6324082, Length=354, Percent_Identity=35.0282485875706, Blast_Score=204, Evalue=3e-53,
Organism=Drosophila melanogaster, GI19922218, Length=500, Percent_Identity=37.2, Blast_Score=276, Evalue=3e-74,
Organism=Drosophila melanogaster, GI19922364, Length=260, Percent_Identity=44.6153846153846, Blast_Score=203, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_ACTP2 (A3N0S3)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053614.1
- ProteinModelPortal:   A3N0S3
- SMR:   A3N0S3
- STRING:   A3N0S3
- GeneID:   4848686
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0913
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- PhylomeDB:   A3N0S3
- ProtClustDB:   PRK00260
- BioCyc:   APLE416269:APL_0913-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 52164; Mature: 52164

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 269-269

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIYNTLKREKEEFKPINPNQVGMYVCGVTVYDLCHFGHGRTFVSFDVIARYLRYLGYN
CCCHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHCCC
LRYVRNITDVDDKIIKRALENNETCDQLVDRMIAEMHKDFDDLNILRPDVEPRATKHIPE
HHEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
IVAMVEKLIANGHAYVAADGDVMFDVESFKKYGALSRQNLEQLQAGARVEIKSVKKNPMD
HHHHHHHHHHCCCEEEEECCCEEEEHHHHHHHCCCCHHHHHHHHCCCCEEEHHHCCCCCE
FVLWKMSKEGEPSWQSPWGNGRPGWHIECSAMNSKELGEHFDIHGGGSDLMFPHHENEIA
EEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCHHCCCCCCCCCCCCCCCCCCCHHHHH
QSCCAHGGDYVNYWLHTGMLTIDDEKMSKSLGNFFTIRTMLEKYESETLRYFFLTAHYRS
HHHHHCCCHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLNYSLDNLDLARSALERLYTSLRGCDLSVEVAGGEQYVEAFKAAMDDDFNTPGALAVLF
HHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHH
EIAREVNKLKTEDMAKANGLAVRLKELAGVLGLLYQDPEAFLQGDADNDEVAEIEALIKQ
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHH
RNEAKAAKNWAVADEVRDKLKAMNIVLEDTPNGTTWRKA
HHHHHHHHCCHHHHHHHHHHHHHHEEEECCCCCCCCCCC
>Mature Secondary Structure
MLKIYNTLKREKEEFKPINPNQVGMYVCGVTVYDLCHFGHGRTFVSFDVIARYLRYLGYN
CCCHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHCCC
LRYVRNITDVDDKIIKRALENNETCDQLVDRMIAEMHKDFDDLNILRPDVEPRATKHIPE
HHEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
IVAMVEKLIANGHAYVAADGDVMFDVESFKKYGALSRQNLEQLQAGARVEIKSVKKNPMD
HHHHHHHHHHCCCEEEEECCCEEEEHHHHHHHCCCCHHHHHHHHCCCCEEEHHHCCCCCE
FVLWKMSKEGEPSWQSPWGNGRPGWHIECSAMNSKELGEHFDIHGGGSDLMFPHHENEIA
EEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCHHCCCCCCCCCCCCCCCCCCCHHHHH
QSCCAHGGDYVNYWLHTGMLTIDDEKMSKSLGNFFTIRTMLEKYESETLRYFFLTAHYRS
HHHHHCCCHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLNYSLDNLDLARSALERLYTSLRGCDLSVEVAGGEQYVEAFKAAMDDDFNTPGALAVLF
HHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHH
EIAREVNKLKTEDMAKANGLAVRLKELAGVLGLLYQDPEAFLQGDADNDEVAEIEALIKQ
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHH
RNEAKAAKNWAVADEVRDKLKAMNIVLEDTPNGTTWRKA
HHHHHHHHCCHHHHHHHHHHHHHHEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA