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The map label for this gene is proB [H]

Identifier: 126208360

GI number: 126208360

Start: 1023621

End: 1024712

Strand: Direct

Name: proB [H]

Synonym: APL_0884

Alternate gene names: 126208360

Gene position: 1023621-1024712 (Clockwise)

Preceding gene: 126208357

Following gene: 126208361

Centisome position: 45.0

GC content: 45.33

Gene sequence:

>1092_bases
ATGCAAAAGACAATTGTGATTAAATTCGGCACCAGTACTCTTACGCACGGAGGAAAGAGTTTAAGCCGTCCTTATATGCT
GGAATTAGTTAAACAAATAGCTCAACTTCATCAACATCATCGAGTGATTGTCGTCACCTCCGGAGCCGCAGCAGCCGGCA
GAGATTATTTAGGTCATCCCGAACTTCCCAAAACACTCGCCTATAAACAAATGCTTGCCGCAGTCGGGCAAAGTCAGCTA
ATCCGTACTTGGGAAAATTTATTCGATATTTACGGCATTCATATCGGGCAAATTTTATTAACCCGTGCCGATATTGATGA
TCGCGAACGTTTTTTAAATGCGCGCGATACCTTAAATGCCTTGTTGGAGCAAAGAATCATTCCTGTGATCAATGAAAACG
ATACGGTTGCCACCGAAGAATTTAAAGTCGGCGATAATGACAATCTTTCCGCATTGGTTGCGATTCTAGCGCATGCCGAT
CAACTTTATCTTTTAACCGACCAAGAAGGCTTATTTGATTCCGATCCGCGTTCAAATCCGCAAGCGAAATTACTGTCCGT
CGTGGAAAAAATCACGCCTGAAATCCGCCAAATGGCGGGCGGTAGCGTCACCGGACTCGGCACCGGCGGTATGAGTACTA
AAATTACCGCCGCCGATATTGCTACGCGTTCCGGTGTCGAAACCGTTATCGCTTCAGGCTCTCGTGCAAACGTATTGGTC
GATTTAGCTAACGGCGAATCCATCGGTAGTAAGTTTTTAGTACAATCCGACCGCTTGGAAGGTCGTAAACAATGGTTATT
CGCCGCTCCGCAAGTCGGCTCGCTTGTGGTGGATAAAGGGGCGGAAGCTGCATTATTACAACATAAATCATTACTGCCGG
TCGGTGTTTCCGAGATTAACGGCGTCTTTAGCCGAGGCGAAGTAGTGAAAATTTTTAACCAAGAAGGTAAAGCGCTTGCT
TTGGGGCTTGCTCGTTATAACAGCGATGCGTTAATTCGTATTAAAGGAAAAAAATCGAAAGAGATTGAGTCTCTGCTCGG
TTATGAATTCGGTTCCGTCGCCGTACATATTGATGAGATGGTCATTTATTAA

Upstream 100 bases:

>100_bases
AAACTTATCTTCATTTTTTCTTCCTTTTCAACAAAATTAGGCGTGATTTTAACAAATAAAAGTGATACTTTGGTGCTAAA
TTTTTTATCGGGATAAAACT

Downstream 100 bases:

>100_bases
AAAAGGATATTTATGGAACTTTCGTTTGATGTTTTAGCGATGTTATTCACGATAGCAACGATCGCCGGCTTTATTGATGC
GATAGCCGGTGGCGGCGGGT

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MQKTIVIKFGTSTLTHGGKSLSRPYMLELVKQIAQLHQHHRVIVVTSGAAAAGRDYLGHPELPKTLAYKQMLAAVGQSQL
IRTWENLFDIYGIHIGQILLTRADIDDRERFLNARDTLNALLEQRIIPVINENDTVATEEFKVGDNDNLSALVAILAHAD
QLYLLTDQEGLFDSDPRSNPQAKLLSVVEKITPEIRQMAGGSVTGLGTGGMSTKITAADIATRSGVETVIASGSRANVLV
DLANGESIGSKFLVQSDRLEGRKQWLFAAPQVGSLVVDKGAEAALLQHKSLLPVGVSEINGVFSRGEVVKIFNQEGKALA
LGLARYNSDALIRIKGKKSKEIESLLGYEFGSVAVHIDEMVIY

Sequences:

>Translated_363_residues
MQKTIVIKFGTSTLTHGGKSLSRPYMLELVKQIAQLHQHHRVIVVTSGAAAAGRDYLGHPELPKTLAYKQMLAAVGQSQL
IRTWENLFDIYGIHIGQILLTRADIDDRERFLNARDTLNALLEQRIIPVINENDTVATEEFKVGDNDNLSALVAILAHAD
QLYLLTDQEGLFDSDPRSNPQAKLLSVVEKITPEIRQMAGGSVTGLGTGGMSTKITAADIATRSGVETVIASGSRANVLV
DLANGESIGSKFLVQSDRLEGRKQWLFAAPQVGSLVVDKGAEAALLQHKSLLPVGVSEINGVFSRGEVVKIFNQEGKALA
LGLARYNSDALIRIKGKKSKEIESLLGYEFGSVAVHIDEMVIY
>Mature_363_residues
MQKTIVIKFGTSTLTHGGKSLSRPYMLELVKQIAQLHQHHRVIVVTSGAAAAGRDYLGHPELPKTLAYKQMLAAVGQSQL
IRTWENLFDIYGIHIGQILLTRADIDDRERFLNARDTLNALLEQRIIPVINENDTVATEEFKVGDNDNLSALVAILAHAD
QLYLLTDQEGLFDSDPRSNPQAKLLSVVEKITPEIRQMAGGSVTGLGTGGMSTKITAADIATRSGVETVIASGSRANVLV
DLANGESIGSKFLVQSDRLEGRKQWLFAAPQVGSLVVDKGAEAALLQHKSLLPVGVSEINGVFSRGEVVKIFNQEGKALA
LGLARYNSDALIRIKGKKSKEIESLLGYEFGSVAVHIDEMVIY

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=285, Percent_Identity=32.280701754386, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI21361368, Length=287, Percent_Identity=32.0557491289199, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI1786437, Length=361, Percent_Identity=60.9418282548476, Blast_Score=456, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI17569969, Length=269, Percent_Identity=33.0855018587361, Blast_Score=127, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17569967, Length=269, Percent_Identity=33.0855018587361, Blast_Score=127, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6320506, Length=411, Percent_Identity=37.2262773722628, Blast_Score=213, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6321822, Length=408, Percent_Identity=34.5588235294118, Blast_Score=198, Evalue=1e-51,
Organism=Drosophila melanogaster, GI21357643, Length=286, Percent_Identity=30.7692307692308, Blast_Score=119, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39392; Mature: 39392

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKTIVIKFGTSTLTHGGKSLSRPYMLELVKQIAQLHQHHRVIVVTSGAAAAGRDYLGHP
CCCEEEEEECCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCC
ELPKTLAYKQMLAAVGQSQLIRTWENLFDIYGIHIGQILLTRADIDDRERFLNARDTLNA
CCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHH
LLEQRIIPVINENDTVATEEFKVGDNDNLSALVAILAHADQLYLLTDQEGLFDSDPRSNP
HHHHCCCEEECCCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCH
QAKLLSVVEKITPEIRQMAGGSVTGLGTGGMSTKITAADIATRSGVETVIASGSRANVLV
HHHHHHHHHHHCHHHHHHCCCCEEEECCCCCCCEEEHHHHHHHCCHHHHHCCCCCCEEEE
DLANGESIGSKFLVQSDRLEGRKQWLFAAPQVGSLVVDKGAEAALLQHKSLLPVGVSEIN
EECCCCCCCCHHEEECHHCCCCHHEEEECCCCCCEEECCCCCHHHHHCCCCCCCCHHHHH
GVFSRGEVVKIFNQEGKALALGLARYNSDALIRIKGKKSKEIESLLGYEFGSVAVHIDEM
HHHCCCCEEEEECCCCCEEEEEEEEECCCEEEEEECCCHHHHHHHHCCCCCCEEEEEEEE
VIY
EEC
>Mature Secondary Structure
MQKTIVIKFGTSTLTHGGKSLSRPYMLELVKQIAQLHQHHRVIVVTSGAAAAGRDYLGHP
CCCEEEEEECCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCC
ELPKTLAYKQMLAAVGQSQLIRTWENLFDIYGIHIGQILLTRADIDDRERFLNARDTLNA
CCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHH
LLEQRIIPVINENDTVATEEFKVGDNDNLSALVAILAHADQLYLLTDQEGLFDSDPRSNP
HHHHCCCEEECCCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCH
QAKLLSVVEKITPEIRQMAGGSVTGLGTGGMSTKITAADIATRSGVETVIASGSRANVLV
HHHHHHHHHHHCHHHHHHCCCCEEEECCCCCCCEEEHHHHHHHCCHHHHHCCCCCCEEEE
DLANGESIGSKFLVQSDRLEGRKQWLFAAPQVGSLVVDKGAEAALLQHKSLLPVGVSEIN
EECCCCCCCCHHEEECHHCCCCHHEEEECCCCCCEEECCCCCHHHHHCCCCCCCCHHHHH
GVFSRGEVVKIFNQEGKALALGLARYNSDALIRIKGKKSKEIESLLGYEFGSVAVHIDEM
HHHCCCCEEEEECCCCCEEEEEEEEECCCEEEEEECCCHHHHHHHHCCCCCCEEEEEEEE
VIY
EEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA