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The map label for this gene is gyrB [H]

Identifier: 126208297

GI number: 126208297

Start: 947874

End: 950306

Strand: Reverse

Name: gyrB [H]

Synonym: APL_0821

Alternate gene names: 126208297

Gene position: 950306-947874 (Counterclockwise)

Preceding gene: 126208298

Following gene: 126208294

Centisome position: 41.78

GC content: 42.95

Gene sequence:

>2433_bases
ATGTCTGAACAAAATCAAACAAATAGTTATGATTCTTCGAGCATTAAAGTCTTACGCGGCTTAGATGCGGTACGTAAACG
TCCGGGTATGTATATCGGTGATACCGATGATGGTACAGGTTTACACCATATGGTGTTTGAAGTTGTAGATAATGCGATCG
ATGAAGCGTTAGCGGGACACTGTAAAGATATTATCATAACCATTCATTCGGATAATTCGGTTTCCGTACAAGATGACGGC
CGCGGTATTCCGGTCGGCATTCATCCGGAAGAGGGCGTATCGGCAGCGGAAGTGATTATGACGGTATTGCATGCCGGCGG
TAAATTTGACGATAACTCCTATAAAGTATCGGGCGGTTTACACGGTGTGGGTGTGTCGGTGGTAAATGCGCTTTCATCAA
AATTACAGCTCACCATTCGCCGTGAAGGTCATGTTCACGAGCAATTTTATAGCTTAGGCGAACCGGATGCGCCATTAACC
GTTATCGGCGATACTGATAAAACCGGTACGATGGTACGTTTCTGGCCGAGCTTAGATATCTTCAAAAATAAAACCGAATT
CGAATATAAAATCCTGTCAAAACGTTTACGTGAGCTTTCTTTCTTAAACTCGGGCGTATCGATTAGATTAATTGACGAAC
GTGACGGCAAAGAAGAACATTTCAAATATGAAGGCGGTATTAAAGCGTACGTTGAGTATTTAAACGAAGGTAAAACCCAT
ATTCATAACACGCCGTTTTATCTTTCAACCGAGAAAGACGGTATCGGTGTTGAAGTGTCATTACAGTGGAACGACAGTTA
TAACGAAAATGTTTACTGTTTTACTAATAATATTCCGCAACGTGATGGTGGTACGCACTTAGCCGGTTTCCGTGGTGCAT
TAACCCGCGCATTAAAAAACTATATGGATAACAGCGGTGTAGTGAAGAAAGCGGATGCGAATATTGATGCGTCAGGGGAT
GATGCGCGTGAAGGTTTGGTTGCGGTAATTTCGGTCAAAGTACCGGATCCGAAATTCTCATCACAAACGAAAGATAAGTT
AGTGTCATCGGAAGTGCGTGGAGCGGTGGAAAGTTCAATGAACGAAGCGTTATCCGAATACTTAGCGGAAAATCCGGACG
ATGCGAAAAATATTGTCACCAAAATTATTGATGCGGCACGAGCACGTGAAGCAGCACGTAAAGCGCGTGAAATGACACGT
CGTAAAGGTGCGCTGGATTTAGGCGGCTTACCGGGTAAATTAGCGGACTGCCAAGAAAAAGACCCAGCGTTGTCGGAACT
TTACTTAGTGGAGGGTGACTCTGCGGGCGGTTCGGCAAAACAAGGTCGTGATCGTAAAAACCAAGCGATTCTGCCGTTAA
AAGGTAAAATCTTAAACGTTGAAAAAGCACGTTTCGATAAAATGCTTTCTTCGCAAGAAGTCGCAACCTTGATTACTGCA
TTAGGCACCGGTATCGGTCGTGATGATTACAATATTGAGAAATTACGTTATCACAAAATCATTATTATGACCGATGCGGA
CGTGGACGGTGCGCATATTCGTACCTTGTTATTAACTTTCTTCTACCGCCAAATGCCGGAATTGATTGAGAACGGTTATG
TTTATATCGCTCAACCGCCGCTTTATAAAGTGAAAAAAGGTAAACAAGAGCGTTATATTCAAGATAACGACGAAATGTCG
CAATATGAAATTGATATTGCTTTGGAAGGTGCAGGTTTATTTGTGAGTGAAGGCGCGCCGGCATTAAGCGGTGTGGCACT
TGAAAGCCTCATTTCACAATATAACGGCGTGCAAAAATTATTTGATCGCTTAAGTCGCTGTTATCCGGTGGCGTTATTAA
ACGAATTAATTTATAGCCCGGCGTTATCGGCTGAATTTGCAAAAGTTCAGGCAAAAGTGACCGCTTGGAATGAGCAGTTT
GTGGCGAAGTTAATGGAAAAAGAAACCGGCGGTAGTTTCTACCGTAGTGAAGTTTTCTTCAATACCGAGCGTCAAGTATA
TGAGCCGGAAATTGTGGTAACGACTCACGGTATGGATACGACTTATCGTCTTGATTTTAACTTTATCAGCAGTAACGAAT
ACGCCAAAATTACCGCATTAGGTAACGAGTTAAACGACTTATTATCACCAAGTGCTTATGTAATGCGCGGTGAACGTCGC
CAAGAAGTGAGCAGCTTTGCCGAAGCGTTAGATTGGTTAGTGCGTGAATCTCGTAAAGGCTTAACCATTCAACGCTATAA
AGGATTAGGCGAGATGAATCCGGAGCAATTATGGGAAACTACAATGGATCCGGTTGCACGTAAGATGTTACAAGTAAATA
TCACCGATGCGATTGCGGCAGACAAATTGTTTAGTACCTTAATGGGCGATGAAGTTGAACCTCGTCGCGACTTTATCGAA
AGCAATGCGTTATATGCTCAGTTAGATATTTAA

Upstream 100 bases:

>100_bases
CTTTTCCGCATTTTTCTGCTATAATTCCAATAATTTTTTGGCTTTAAATTTATTCGTAACAAATAAGCCTTTTCCATTTC
ATAAAGTATAGGAATAACGA

Downstream 100 bases:

>100_bases
TCTAAGCTAAATAAAAACCCCAAAGTATTTTGCTTTGGGGTTTGTTTTGGCATTTTATGTAAAAAAAGACCGCTTGTGTA
AGTGGTCTTTTTCATCAAAT

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 810; Mature: 809

Protein sequence:

>810_residues
MSEQNQTNSYDSSSIKVLRGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCKDIIITIHSDNSVSVQDDG
RGIPVGIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSSKLQLTIRREGHVHEQFYSLGEPDAPLT
VIGDTDKTGTMVRFWPSLDIFKNKTEFEYKILSKRLRELSFLNSGVSIRLIDERDGKEEHFKYEGGIKAYVEYLNEGKTH
IHNTPFYLSTEKDGIGVEVSLQWNDSYNENVYCFTNNIPQRDGGTHLAGFRGALTRALKNYMDNSGVVKKADANIDASGD
DAREGLVAVISVKVPDPKFSSQTKDKLVSSEVRGAVESSMNEALSEYLAENPDDAKNIVTKIIDAARAREAARKAREMTR
RKGALDLGGLPGKLADCQEKDPALSELYLVEGDSAGGSAKQGRDRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITA
LGTGIGRDDYNIEKLRYHKIIIMTDADVDGAHIRTLLLTFFYRQMPELIENGYVYIAQPPLYKVKKGKQERYIQDNDEMS
QYEIDIALEGAGLFVSEGAPALSGVALESLISQYNGVQKLFDRLSRCYPVALLNELIYSPALSAEFAKVQAKVTAWNEQF
VAKLMEKETGGSFYRSEVFFNTERQVYEPEIVVTTHGMDTTYRLDFNFISSNEYAKITALGNELNDLLSPSAYVMRGERR
QEVSSFAEALDWLVRESRKGLTIQRYKGLGEMNPEQLWETTMDPVARKMLQVNITDAIAADKLFSTLMGDEVEPRRDFIE
SNALYAQLDI

Sequences:

>Translated_810_residues
MSEQNQTNSYDSSSIKVLRGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCKDIIITIHSDNSVSVQDDG
RGIPVGIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSSKLQLTIRREGHVHEQFYSLGEPDAPLT
VIGDTDKTGTMVRFWPSLDIFKNKTEFEYKILSKRLRELSFLNSGVSIRLIDERDGKEEHFKYEGGIKAYVEYLNEGKTH
IHNTPFYLSTEKDGIGVEVSLQWNDSYNENVYCFTNNIPQRDGGTHLAGFRGALTRALKNYMDNSGVVKKADANIDASGD
DAREGLVAVISVKVPDPKFSSQTKDKLVSSEVRGAVESSMNEALSEYLAENPDDAKNIVTKIIDAARAREAARKAREMTR
RKGALDLGGLPGKLADCQEKDPALSELYLVEGDSAGGSAKQGRDRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITA
LGTGIGRDDYNIEKLRYHKIIIMTDADVDGAHIRTLLLTFFYRQMPELIENGYVYIAQPPLYKVKKGKQERYIQDNDEMS
QYEIDIALEGAGLFVSEGAPALSGVALESLISQYNGVQKLFDRLSRCYPVALLNELIYSPALSAEFAKVQAKVTAWNEQF
VAKLMEKETGGSFYRSEVFFNTERQVYEPEIVVTTHGMDTTYRLDFNFISSNEYAKITALGNELNDLLSPSAYVMRGERR
QEVSSFAEALDWLVRESRKGLTIQRYKGLGEMNPEQLWETTMDPVARKMLQVNITDAIAADKLFSTLMGDEVEPRRDFIE
SNALYAQLDI
>Mature_809_residues
SEQNQTNSYDSSSIKVLRGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCKDIIITIHSDNSVSVQDDGR
GIPVGIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSSKLQLTIRREGHVHEQFYSLGEPDAPLTV
IGDTDKTGTMVRFWPSLDIFKNKTEFEYKILSKRLRELSFLNSGVSIRLIDERDGKEEHFKYEGGIKAYVEYLNEGKTHI
HNTPFYLSTEKDGIGVEVSLQWNDSYNENVYCFTNNIPQRDGGTHLAGFRGALTRALKNYMDNSGVVKKADANIDASGDD
AREGLVAVISVKVPDPKFSSQTKDKLVSSEVRGAVESSMNEALSEYLAENPDDAKNIVTKIIDAARAREAARKAREMTRR
KGALDLGGLPGKLADCQEKDPALSELYLVEGDSAGGSAKQGRDRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITAL
GTGIGRDDYNIEKLRYHKIIIMTDADVDGAHIRTLLLTFFYRQMPELIENGYVYIAQPPLYKVKKGKQERYIQDNDEMSQ
YEIDIALEGAGLFVSEGAPALSGVALESLISQYNGVQKLFDRLSRCYPVALLNELIYSPALSAEFAKVQAKVTAWNEQFV
AKLMEKETGGSFYRSEVFFNTERQVYEPEIVVTTHGMDTTYRLDFNFISSNEYAKITALGNELNDLLSPSAYVMRGERRQ
EVSSFAEALDWLVRESRKGLTIQRYKGLGEMNPEQLWETTMDPVARKMLQVNITDAIAADKLFSTLMGDEVEPRRDFIES
NALYAQLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=551, Percent_Identity=25.4083484573503, Blast_Score=130, Evalue=6e-30,
Organism=Homo sapiens, GI19913406, Length=550, Percent_Identity=24.9090909090909, Blast_Score=122, Evalue=1e-27,
Organism=Escherichia coli, GI48994957, Length=800, Percent_Identity=75.125, Blast_Score=1227, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=556, Percent_Identity=41.1870503597122, Blast_Score=405, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17535065, Length=558, Percent_Identity=26.3440860215054, Blast_Score=140, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI212645845, Length=519, Percent_Identity=24.6628131021195, Blast_Score=133, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI212645657, Length=213, Percent_Identity=30.0469483568075, Blast_Score=76, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6324241, Length=586, Percent_Identity=26.962457337884, Blast_Score=120, Evalue=7e-28,
Organism=Drosophila melanogaster, GI17136538, Length=550, Percent_Identity=25.8181818181818, Blast_Score=138, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 90079; Mature: 89947

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQNQTNSYDSSSIKVLRGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGH
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCC
CKDIIITIHSDNSVSVQDDGRGIPVGIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGL
CCEEEEEEECCCEEEECCCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCC
HGVGVSVVNALSSKLQLTIRREGHVHEQFYSLGEPDAPLTVIGDTDKTGTMVRFWPSLDI
CHHHHHHHHHHHHHEEEEEECCCCHHHHHHHCCCCCCCEEEECCCCCCCCEEEEECCCHH
FKNKTEFEYKILSKRLRELSFLNSGVSIRLIDERDGKEEHFKYEGGIKAYVEYLNEGKTH
HCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHEEECCCHHHHHHHHHCCCCE
IHNTPFYLSTEKDGIGVEVSLQWNDSYNENVYCFTNNIPQRDGGTHLAGFRGALTRALKN
ECCCCEEEEECCCCCEEEEEEEECCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
YMDNSGVVKKADANIDASGDDAREGLVAVISVKVPDPKFSSQTKDKLVSSEVRGAVESSM
HHCCCCCEEECCCCCCCCCCCHHCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
NEALSEYLAENPDDAKNIVTKIIDAARAREAARKAREMTRRKGALDLGGLPGKLADCQEK
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
DPALSELYLVEGDSAGGSAKQGRDRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITA
CCCCEEEEEEECCCCCCCCCCCCCCCCCEEEECCCCEECHHHHHHHHHHHHHHHHHHHHH
LGTGIGRDDYNIEKLRYHKIIIMTDADVDGAHIRTLLLTFFYRQMPELIENGYVYIAQPP
HHCCCCCCCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCC
LYKVKKGKQERYIQDNDEMSQYEIDIALEGAGLFVSEGAPALSGVALESLISQYNGVQKL
CHHHHCCCCCCCCCCCCCCCEEEEEEEEECCCEEEECCCCHHHHHHHHHHHHHHCCHHHH
FDRLSRCYPVALLNELIYSPALSAEFAKVQAKVTAWNEQFVAKLMEKETGGSFYRSEVFF
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHEEHHHHHHHHHHHHHCCCCCEEEHHEEE
NTERQVYEPEIVVTTHGMDTTYRLDFNFISSNEYAKITALGNELNDLLSPSAYVMRGERR
CCCCEEECCEEEEEECCCCEEEEEEEEEECCCCEEEEEECCHHHHHHHCCCHHHHCCHHH
QEVSSFAEALDWLVRESRKGLTIQRYKGLGEMNPEQLWETTMDPVARKMLQVNITDAIAA
HHHHHHHHHHHHHHHHHCCCCEEHHHCCCCCCCHHHHHHHHHHHHHHHHHEECHHHHHHH
DKLFSTLMGDEVEPRRDFIESNALYAQLDI
HHHHHHHHCCCCCHHHHHHHCCCEEEEECC
>Mature Secondary Structure 
SEQNQTNSYDSSSIKVLRGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGH
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCC
CKDIIITIHSDNSVSVQDDGRGIPVGIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGL
CCEEEEEEECCCEEEECCCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCC
HGVGVSVVNALSSKLQLTIRREGHVHEQFYSLGEPDAPLTVIGDTDKTGTMVRFWPSLDI
CHHHHHHHHHHHHHEEEEEECCCCHHHHHHHCCCCCCCEEEECCCCCCCCEEEEECCCHH
FKNKTEFEYKILSKRLRELSFLNSGVSIRLIDERDGKEEHFKYEGGIKAYVEYLNEGKTH
HCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHEEECCCHHHHHHHHHCCCCE
IHNTPFYLSTEKDGIGVEVSLQWNDSYNENVYCFTNNIPQRDGGTHLAGFRGALTRALKN
ECCCCEEEEECCCCCEEEEEEEECCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
YMDNSGVVKKADANIDASGDDAREGLVAVISVKVPDPKFSSQTKDKLVSSEVRGAVESSM
HHCCCCCEEECCCCCCCCCCCHHCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
NEALSEYLAENPDDAKNIVTKIIDAARAREAARKAREMTRRKGALDLGGLPGKLADCQEK
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
DPALSELYLVEGDSAGGSAKQGRDRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITA
CCCCEEEEEEECCCCCCCCCCCCCCCCCEEEECCCCEECHHHHHHHHHHHHHHHHHHHHH
LGTGIGRDDYNIEKLRYHKIIIMTDADVDGAHIRTLLLTFFYRQMPELIENGYVYIAQPP
HHCCCCCCCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCC
LYKVKKGKQERYIQDNDEMSQYEIDIALEGAGLFVSEGAPALSGVALESLISQYNGVQKL
CHHHHCCCCCCCCCCCCCCCEEEEEEEEECCCEEEECCCCHHHHHHHHHHHHHHCCHHHH
FDRLSRCYPVALLNELIYSPALSAEFAKVQAKVTAWNEQFVAKLMEKETGGSFYRSEVFF
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHEEHHHHHHHHHHHHHCCCCCEEEHHEEE
NTERQVYEPEIVVTTHGMDTTYRLDFNFISSNEYAKITALGNELNDLLSPSAYVMRGERR
CCCCEEECCEEEEEECCCCEEEEEEEEEECCCCEEEEEECCHHHHHHHCCCHHHHCCHHH
QEVSSFAEALDWLVRESRKGLTIQRYKGLGEMNPEQLWETTMDPVARKMLQVNITDAIAA
HHHHHHHHHHHHHHHHHCCCCEEHHHCCCCCCCHHHHHHHHHHHHHHHHHEECHHHHHHH
DKLFSTLMGDEVEPRRDFIESNALYAQLDI
HHHHHHHHCCCCCHHHHHHHCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]