Click here to switch to the map view.

The map label for this gene is cafA [H]

Identifier: 126208286

GI number: 126208286

Start: 932365

End: 933840

Strand: Reverse

Name: cafA [H]

Synonym: APL_0810

Alternate gene names: 126208286

Gene position: 933840-932365 (Counterclockwise)

Preceding gene: 126208288

Following gene: 126208285

Centisome position: 41.06

GC content: 42.48

Gene sequence:

>1476_bases
ATGAGCGGTGCAGAATTATTAGTAAACGTGACACCGAGTGAGACTCGGGTGGCGTTAGTTGAGAACGGATTGTTAAAAGA
GCTTCATATTGAGCGTGAAGCGAAGCGTGGGATTGTCGGTAATATTTATAAAGGACGTGTGACACGAGTGTTGCCGGGAA
TGCAGTCGGCATTTGTGGATATCGGCTTGGAAAAAGCGGCATTTTTACACGCTTCCGACATTGTTTCGCATACGGAATGT
GTCGATGAAAACGAGAAGAAACAGTTTGTCGTAAAAGATATTGCAGAGCTGGTACGTGAAGGACAAGACATTATCGTACA
AGTGGTGAAAGACCCGATTAGTACTAAAGGTGCACGTTTAACCACCGATATTACTTTGCCTTCTCGCTATTTGGTGTTTA
TGCCGGAAAATAGCCACGTGGGCGTTTCGCAACGTATCGAAAGCGAGGAAGAGCGTAATCGCTTAAAAGCGTTGGTTGAG
CCTTATTGCGATGAACTTGGCGGCTTTATTGTGCGTACGGCGGCGGAAGAAGTTTCGGAAGAAAGTTTGCAACAAGATAC
GATTTTCTTGAAACGGTTATGGCGTAAAATTTTAGAACGTAAGGCAAAATATCCGGTTAAATCAATGTTATACGGTGAGC
TTGCTCTAGCACAACGAGTCTTACGTGATTTTGTCGGTACACATATCAGTTCGATTCGCATTGACTCAAAAATGACCTAT
GAACAAGTTAAAGAGTTTTTAGCCGAGTTTATGCCGGAACTGACTGAAAACGTGAGTCTATATAGCGGTAGCCAAACGTT
ATTTGATGCCTACGGTGTGGAAGATGCGATCCAAAAAGCGTTGGATAAACGAGTCGATTTAAAATCGGGCGGTTATTTAA
TTATCGAACAAACCGAAGCGATGACTACGATTGATATTAATACCGGTGCGTTTGTCGGTCATCGCAATTTAGCGCATACG
ATTTTTAATACCAATATTGAAGCGACACAAGCAATTGCACATCAGTTACAACTGCGTAATTTAGGCGGCATTATCATTAT
TGATTTTATTGATATGCAAGAAGAAGAACACCGTGAACGTGTGCTGCAGTCGTTACAAGAAGCGTTAAAAGGCGACCGAG
TGAAAACCAATGTGAACGGTTTTACCCAACTTGGCTTGGTCGAAATGACTCGTAAACGTACCCGAGAAAGTTTGGAACGT
GTGCTTTGTTGCGATTGTCCGGCGTGTAAAGGGCGAGGCACGATTAAAACGGTTGAAACGGTTTGTTATGAAATTCTGCG
TGAGATTGTGCGTGTTCATCACTTGTTTAAAACCGAAAAAGTGCGAGTGTACGCTTCAAAAGAAGTGGCGGAATATTTAA
TTAACGAAGAAACGCACGCTTCGATTGCCGAACTCGAAGCCTTTATCGGTAAAACCATCGAAATCAAATTAGAGCCTTAC
TATCATCAAGATCAATTTGATGTTGTCGTGATGTAA

Upstream 100 bases:

>100_bases
GCCAAAATTTTACTACTTATTTGATCTTGGTTTTTGTTTTCTTACTTCGTCAATGTTAAACTTTAGTGAATATGATTTAA
CGACTTAGAGAAAAATAACA

Downstream 100 bases:

>100_bases
TGGGTAAGCGGTCGGATTTTACGAAAGTTTTGCAACTTTTTGCGAAAATCCGACCGCTTGCTTCCTACCCAAAATCTGCA
AAATCGCTTATAATCGCTTT

Product: ribonuclease G

Products: NA

Alternate protein names: RNase G; Cytoplasmic axial filament protein [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MSGAELLVNVTPSETRVALVENGLLKELHIEREAKRGIVGNIYKGRVTRVLPGMQSAFVDIGLEKAAFLHASDIVSHTEC
VDENEKKQFVVKDIAELVREGQDIIVQVVKDPISTKGARLTTDITLPSRYLVFMPENSHVGVSQRIESEEERNRLKALVE
PYCDELGGFIVRTAAEEVSEESLQQDTIFLKRLWRKILERKAKYPVKSMLYGELALAQRVLRDFVGTHISSIRIDSKMTY
EQVKEFLAEFMPELTENVSLYSGSQTLFDAYGVEDAIQKALDKRVDLKSGGYLIIEQTEAMTTIDINTGAFVGHRNLAHT
IFNTNIEATQAIAHQLQLRNLGGIIIIDFIDMQEEEHRERVLQSLQEALKGDRVKTNVNGFTQLGLVEMTRKRTRESLER
VLCCDCPACKGRGTIKTVETVCYEILREIVRVHHLFKTEKVRVYASKEVAEYLINEETHASIAELEAFIGKTIEIKLEPY
YHQDQFDVVVM

Sequences:

>Translated_491_residues
MSGAELLVNVTPSETRVALVENGLLKELHIEREAKRGIVGNIYKGRVTRVLPGMQSAFVDIGLEKAAFLHASDIVSHTEC
VDENEKKQFVVKDIAELVREGQDIIVQVVKDPISTKGARLTTDITLPSRYLVFMPENSHVGVSQRIESEEERNRLKALVE
PYCDELGGFIVRTAAEEVSEESLQQDTIFLKRLWRKILERKAKYPVKSMLYGELALAQRVLRDFVGTHISSIRIDSKMTY
EQVKEFLAEFMPELTENVSLYSGSQTLFDAYGVEDAIQKALDKRVDLKSGGYLIIEQTEAMTTIDINTGAFVGHRNLAHT
IFNTNIEATQAIAHQLQLRNLGGIIIIDFIDMQEEEHRERVLQSLQEALKGDRVKTNVNGFTQLGLVEMTRKRTRESLER
VLCCDCPACKGRGTIKTVETVCYEILREIVRVHHLFKTEKVRVYASKEVAEYLINEETHASIAELEAFIGKTIEIKLEPY
YHQDQFDVVVM
>Mature_490_residues
SGAELLVNVTPSETRVALVENGLLKELHIEREAKRGIVGNIYKGRVTRVLPGMQSAFVDIGLEKAAFLHASDIVSHTECV
DENEKKQFVVKDIAELVREGQDIIVQVVKDPISTKGARLTTDITLPSRYLVFMPENSHVGVSQRIESEEERNRLKALVEP
YCDELGGFIVRTAAEEVSEESLQQDTIFLKRLWRKILERKAKYPVKSMLYGELALAQRVLRDFVGTHISSIRIDSKMTYE
QVKEFLAEFMPELTENVSLYSGSQTLFDAYGVEDAIQKALDKRVDLKSGGYLIIEQTEAMTTIDINTGAFVGHRNLAHTI
FNTNIEATQAIAHQLQLRNLGGIIIIDFIDMQEEEHRERVLQSLQEALKGDRVKTNVNGFTQLGLVEMTRKRTRESLERV
LCCDCPACKGRGTIKTVETVCYEILREIVRVHHLFKTEKVRVYASKEVAEYLINEETHASIAELEAFIGKTIEIKLEPYY
HQDQFDVVVM

Specific function: Involved in the processing of the 5'-end of 16S rRNA. Could be involved in chromosome segregation and cell division. It may be one of the components of the cytoplasmic axial filaments bundles or merely regulate the formation of this structure [H]

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm, cytoskeleton [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI87082235, Length=488, Percent_Identity=69.672131147541, Blast_Score=694, Evalue=0.0,
Organism=Escherichia coli, GI1787325, Length=458, Percent_Identity=36.6812227074236, Blast_Score=280, Evalue=2e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: 3.1.4.- [C]

Molecular weight: Translated: 55705; Mature: 55574

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGAELLVNVTPSETRVALVENGLLKELHIEREAKRGIVGNIYKGRVTRVLPGMQSAFVD
CCCCCEEEEECCCCCEEEHHHCCHHHHHHHHHHHHCCCCCCCHHCHHHHHCCCHHHHHHH
IGLEKAAFLHASDIVSHTECVDENEKKQFVVKDIAELVREGQDIIVQVVKDPISTKGARL
CCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEE
TTDITLPSRYLVFMPENSHVGVSQRIESEEERNRLKALVEPYCDELGGFIVRTAAEEVSE
EEEEECCCEEEEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
ESLQQDTIFLKRLWRKILERKAKYPVKSMLYGELALAQRVLRDFVGTHISSIRIDSKMTY
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHEEECCCCCH
EQVKEFLAEFMPELTENVSLYSGSQTLFDAYGVEDAIQKALDKRVDLKSGGYLIIEQTEA
HHHHHHHHHHHHHHHCCCEEECCCHHHHHHCCHHHHHHHHHHHCCCCCCCCEEEEEECCC
MTTIDINTGAFVGHRNLAHTIFNTNIEATQAIAHQLQLRNLGGIIIIDFIDMQEEEHRER
EEEEECCCCCEECCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHH
VLQSLQEALKGDRVKTNVNGFTQLGLVEMTRKRTRESLERVLCCDCPACKGRGTIKTVET
HHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
VCYEILREIVRVHHLFKTEKVRVYASKEVAEYLINEETHASIAELEAFIGKTIEIKLEPY
HHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEECCC
YHQDQFDVVVM
CCCCCEEEEEC
>Mature Secondary Structure 
SGAELLVNVTPSETRVALVENGLLKELHIEREAKRGIVGNIYKGRVTRVLPGMQSAFVD
CCCCEEEEECCCCCEEEHHHCCHHHHHHHHHHHHCCCCCCCHHCHHHHHCCCHHHHHHH
IGLEKAAFLHASDIVSHTECVDENEKKQFVVKDIAELVREGQDIIVQVVKDPISTKGARL
CCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEE
TTDITLPSRYLVFMPENSHVGVSQRIESEEERNRLKALVEPYCDELGGFIVRTAAEEVSE
EEEEECCCEEEEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
ESLQQDTIFLKRLWRKILERKAKYPVKSMLYGELALAQRVLRDFVGTHISSIRIDSKMTY
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHEEECCCCCH
EQVKEFLAEFMPELTENVSLYSGSQTLFDAYGVEDAIQKALDKRVDLKSGGYLIIEQTEA
HHHHHHHHHHHHHHHCCCEEECCCHHHHHHCCHHHHHHHHHHHCCCCCCCCEEEEEECCC
MTTIDINTGAFVGHRNLAHTIFNTNIEATQAIAHQLQLRNLGGIIIIDFIDMQEEEHRER
EEEEECCCCCEECCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHH
VLQSLQEALKGDRVKTNVNGFTQLGLVEMTRKRTRESLERVLCCDCPACKGRGTIKTVET
HHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
VCYEILREIVRVHHLFKTEKVRVYASKEVAEYLINEETHASIAELEAFIGKTIEIKLEPY
HHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEECCC
YHQDQFDVVVM
CCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]