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The map label for this gene is lysS

Identifier: 126208285

GI number: 126208285

Start: 930728

End: 932230

Strand: Reverse

Name: lysS

Synonym: APL_0809

Alternate gene names: 126208285

Gene position: 932230-930728 (Counterclockwise)

Preceding gene: 126208286

Following gene: 126208284

Centisome position: 40.99

GC content: 41.45

Gene sequence:

>1503_bases
ATGTCCGAAGTTGAACATCAAGAATTAGATCTTAACGGTGAAATGTTAGCTCGCCGTGAGAAATTAGCAAAATTACGTGA
ACAAGGTAATCCGTTCCCAAATACTTTCCGTCGTGATGCTTACGCAGAAAAATTACACGCACAATACGATGAAGTAGAGG
GTGAAGCGTTAAAAGAACAAGATATTCAAGTGAAAGTTGCCGGTCGTATTATGCTTAAACGTGTAATGGGTAAGGCATCT
TTCTTTACCATTCAAGATGTAAGCGGTCAAATCCAGCTTTATGTTGCACGTGATAACCTTGCGGAAGGTGTTTATGCGGA
TAAAGTCAGTATGTGGGATTTAGGCGATATCGTCGGTGTTGCCGGTACGCTTTTCAAAACGAAAACGGGCGAATTAACCG
TTCGTTGTTCGGAAGTGGAGTTATTAACTAAATCACTTCGTCCGTTACCAAATAAAGTACAAGGCTTAACCGACCAAGAA
ACACGTTATCGTCAGCGCTATTTAGACTTAATTTCTAATGAAGAATCTCGCCGCACGTTTATGATTCGCTCAAAAGTGGT
TTCAGGCATTCGTCAATTCTTCTTAGAGAAAGACTTTATTGAAGTTGAAACGCCAATGTTACAAGTGATTCCGGGCGGTG
CGGCTGCAAAACCGTTTATCACTCACCATAATGCGTTAGACGTAGATATGTATTTACGTATCGCACCTGAGCTTTACTTA
AAACGTTTAGTAGTTGGCGGTTTTGAGCGTGTATTCGAATTAAACCGTAACTTCCGTAATGAAGGTGTTTCAGTGCGTCA
CAACCCTGAGTTCACTATGATCGAATACTATCAAGCGTATGCGGATTATCACGATTTAATGGATAACACCGAAGAATTAT
TACGTAAATTAGCGATTGATATTCTTGGCACGACAACCGTTCCTTACGGTGAATACGTGTTCGATTTCGGTAAACCGTTT
GAACGTATCACAATGCACGATGCAATCGTGAAATACGGTAACGGCATTACACGTGAAGATTTAGACAGCTTTGAAAAATC
AGTAGAAATTGCAAAAGGCTTAGGTATTGAAATCCAAAAATCTTGGGGCTTAGGTTCTGTGGTTAATGCGATTTTTGAAG
AAGTTGCGGAACATCAATTAATTCAACCGACCTTCTTAATGGCACATCCGGCAGAAATTTCACCGCTTGCGCGCCGTAAT
GATGAAAACCCTGAAGTAACTGATCGTTTCGAATTATTTATCGGTGGTCGTGAAATCGGTAACGGTTTCTCAGAATTAAA
TGATGCGGAAGACCAAGCAGAGCGTTTTGATGCACAAGTGGCAGCAAAAGATGCGGGTGATGATGAAGCGATGTTTAAAG
ATGAAGACTTCGTTGTTGCGTTAGAACACGGTTTACCGCCGACAGCCGGCGAAGGTTTAGGTATCGACCGTTTAGCAATG
ATTTTTGCAAACGCACCGTCAATTCGTGATGTGATCTTATTCCCTGCGATGCGTCAGAAATAA

Upstream 100 bases:

>100_bases
ACTTTTTGCGAAAATCCGACCGCTTGCTTCCTACCCAAAATCTGCAAAATCGCTTATAATCGCTTTTATTTTTCGAATTT
ATCAAGCAAAAGGTTAAAAA

Downstream 100 bases:

>100_bases
TTTTATTTGATACGGAAAAAAGCACACTTTCGAGTGTGCTTTTTTGATCTTATAAGAGGTTAAAAATCACTAGAATCTTA
CAAATTGAGAAGGAAAAACA

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MSEVEHQELDLNGEMLARREKLAKLREQGNPFPNTFRRDAYAEKLHAQYDEVEGEALKEQDIQVKVAGRIMLKRVMGKAS
FFTIQDVSGQIQLYVARDNLAEGVYADKVSMWDLGDIVGVAGTLFKTKTGELTVRCSEVELLTKSLRPLPNKVQGLTDQE
TRYRQRYLDLISNEESRRTFMIRSKVVSGIRQFFLEKDFIEVETPMLQVIPGGAAAKPFITHHNALDVDMYLRIAPELYL
KRLVVGGFERVFELNRNFRNEGVSVRHNPEFTMIEYYQAYADYHDLMDNTEELLRKLAIDILGTTTVPYGEYVFDFGKPF
ERITMHDAIVKYGNGITREDLDSFEKSVEIAKGLGIEIQKSWGLGSVVNAIFEEVAEHQLIQPTFLMAHPAEISPLARRN
DENPEVTDRFELFIGGREIGNGFSELNDAEDQAERFDAQVAAKDAGDDEAMFKDEDFVVALEHGLPPTAGEGLGIDRLAM
IFANAPSIRDVILFPAMRQK

Sequences:

>Translated_500_residues
MSEVEHQELDLNGEMLARREKLAKLREQGNPFPNTFRRDAYAEKLHAQYDEVEGEALKEQDIQVKVAGRIMLKRVMGKAS
FFTIQDVSGQIQLYVARDNLAEGVYADKVSMWDLGDIVGVAGTLFKTKTGELTVRCSEVELLTKSLRPLPNKVQGLTDQE
TRYRQRYLDLISNEESRRTFMIRSKVVSGIRQFFLEKDFIEVETPMLQVIPGGAAAKPFITHHNALDVDMYLRIAPELYL
KRLVVGGFERVFELNRNFRNEGVSVRHNPEFTMIEYYQAYADYHDLMDNTEELLRKLAIDILGTTTVPYGEYVFDFGKPF
ERITMHDAIVKYGNGITREDLDSFEKSVEIAKGLGIEIQKSWGLGSVVNAIFEEVAEHQLIQPTFLMAHPAEISPLARRN
DENPEVTDRFELFIGGREIGNGFSELNDAEDQAERFDAQVAAKDAGDDEAMFKDEDFVVALEHGLPPTAGEGLGIDRLAM
IFANAPSIRDVILFPAMRQK
>Mature_499_residues
SEVEHQELDLNGEMLARREKLAKLREQGNPFPNTFRRDAYAEKLHAQYDEVEGEALKEQDIQVKVAGRIMLKRVMGKASF
FTIQDVSGQIQLYVARDNLAEGVYADKVSMWDLGDIVGVAGTLFKTKTGELTVRCSEVELLTKSLRPLPNKVQGLTDQET
RYRQRYLDLISNEESRRTFMIRSKVVSGIRQFFLEKDFIEVETPMLQVIPGGAAAKPFITHHNALDVDMYLRIAPELYLK
RLVVGGFERVFELNRNFRNEGVSVRHNPEFTMIEYYQAYADYHDLMDNTEELLRKLAIDILGTTTVPYGEYVFDFGKPFE
RITMHDAIVKYGNGITREDLDSFEKSVEIAKGLGIEIQKSWGLGSVVNAIFEEVAEHQLIQPTFLMAHPAEISPLARRND
ENPEVTDRFELFIGGREIGNGFSELNDAEDQAERFDAQVAAKDAGDDEAMFKDEDFVVALEHGLPPTAGEGLGIDRLAMI
FANAPSIRDVILFPAMRQK

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI5031815, Length=510, Percent_Identity=42.9411764705882, Blast_Score=393, Evalue=1e-109,
Organism=Homo sapiens, GI194272210, Length=510, Percent_Identity=42.9411764705882, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI45439306, Length=472, Percent_Identity=22.0338983050847, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI40789249, Length=361, Percent_Identity=25.4847645429363, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI226491502, Length=461, Percent_Identity=23.4273318872017, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1789256, Length=490, Percent_Identity=70, Blast_Score=726, Evalue=0.0,
Organism=Escherichia coli, GI1790571, Length=502, Percent_Identity=67.3306772908366, Blast_Score=720, Evalue=0.0,
Organism=Escherichia coli, GI87082379, Length=317, Percent_Identity=30.9148264984227, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI1788173, Length=310, Percent_Identity=26.1290322580645, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17535925, Length=489, Percent_Identity=43.1492842535787, Blast_Score=370, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI71994340, Length=489, Percent_Identity=43.1492842535787, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17535927, Length=489, Percent_Identity=43.1492842535787, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17551876, Length=472, Percent_Identity=23.9406779661017, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI32566633, Length=283, Percent_Identity=26.5017667844523, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320242, Length=514, Percent_Identity=41.8287937743191, Blast_Score=384, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6324256, Length=477, Percent_Identity=29.1404612159329, Blast_Score=183, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6323011, Length=485, Percent_Identity=24.3298969072165, Blast_Score=101, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24640851, Length=515, Percent_Identity=41.1650485436893, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24640849, Length=515, Percent_Identity=41.1650485436893, Blast_Score=384, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24584738, Length=296, Percent_Identity=29.0540540540541, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17136276, Length=465, Percent_Identity=23.2258064516129, Blast_Score=87, Evalue=4e-17,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYK_ACTP2 (A3N0G9)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053510.1
- ProteinModelPortal:   A3N0G9
- SMR:   A3N0G9
- STRING:   A3N0G9
- GeneID:   4849593
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0809
- eggNOG:   COG1190
- HOGENOM:   HBG631383
- OMA:   RNEGMDH
- PhylomeDB:   A3N0G9
- ProtClustDB:   PRK00484
- BioCyc:   APLE416269:APL_0809-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00252
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF4
- PRINTS:   PR00982
- TIGRFAMs:   TIGR00499

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.6

Molecular weight: Translated: 56719; Mature: 56588

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVEHQELDLNGEMLARREKLAKLREQGNPFPNTFRRDAYAEKLHAQYDEVEGEALKEQ
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCC
DIQVKVAGRIMLKRVMGKASFFTIQDVSGQIQLYVARDNLAEGVYADKVSMWDLGDIVGV
CCEEEHHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHH
AGTLFKTKTGELTVRCSEVELLTKSLRPLPNKVQGLTDQETRYRQRYLDLISNEESRRTF
HHHHHEECCCCEEEEEHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHCCCCHHHHH
MIRSKVVSGIRQFFLEKDFIEVETPMLQVIPGGAAAKPFITHHNALDVDMYLRIAPELYL
HHHHHHHHHHHHHHHCCCCHHHCCCEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHHHH
KRLVVGGFERVFELNRNFRNEGVSVRHNPEFTMIEYYQAYADYHDLMDNTEELLRKLAID
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
ILGTTTVPYGEYVFDFGKPFERITMHDAIVKYGNGITREDLDSFEKSVEIAKGLGIEIQK
HHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEC
SWGLGSVVNAIFEEVAEHQLIQPTFLMAHPAEISPLARRNDENPEVTDRFELFIGGREIG
CCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHCCCCCCCHHCEEEEEECCHHHC
NGFSELNDAEDQAERFDAQVAAKDAGDDEAMFKDEDFVVALEHGLPPTAGEGLGIDRLAM
CCHHHCCCHHHHHHHHHHHHHHCCCCCCCCEECCCCEEEEEECCCCCCCCCCCCHHHHHH
IFANAPSIRDVILFPAMRQK
HHHCCCCHHHHEECCCCCCC
>Mature Secondary Structure 
SEVEHQELDLNGEMLARREKLAKLREQGNPFPNTFRRDAYAEKLHAQYDEVEGEALKEQ
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCC
DIQVKVAGRIMLKRVMGKASFFTIQDVSGQIQLYVARDNLAEGVYADKVSMWDLGDIVGV
CCEEEHHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHH
AGTLFKTKTGELTVRCSEVELLTKSLRPLPNKVQGLTDQETRYRQRYLDLISNEESRRTF
HHHHHEECCCCEEEEEHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHCCCCHHHHH
MIRSKVVSGIRQFFLEKDFIEVETPMLQVIPGGAAAKPFITHHNALDVDMYLRIAPELYL
HHHHHHHHHHHHHHHCCCCHHHCCCEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHHHH
KRLVVGGFERVFELNRNFRNEGVSVRHNPEFTMIEYYQAYADYHDLMDNTEELLRKLAID
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
ILGTTTVPYGEYVFDFGKPFERITMHDAIVKYGNGITREDLDSFEKSVEIAKGLGIEIQK
HHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEC
SWGLGSVVNAIFEEVAEHQLIQPTFLMAHPAEISPLARRNDENPEVTDRFELFIGGREIG
CCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHCCCCCCCHHCEEEEEECCHHHC
NGFSELNDAEDQAERFDAQVAAKDAGDDEAMFKDEDFVVALEHGLPPTAGEGLGIDRLAM
CCHHHCCCHHHHHHHHHHHHHHCCCCCCCCEECCCCEEEEEECCCCCCCCCCCCHHHHHH
IFANAPSIRDVILFPAMRQK
HHHCCCCHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA