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The map label for this gene is pyrF

Identifier: 126208216

GI number: 126208216

Start: 842282

End: 842974

Strand: Reverse

Name: pyrF

Synonym: APL_0736

Alternate gene names: 126208216

Gene position: 842974-842282 (Counterclockwise)

Preceding gene: 126208217

Following gene: 126208215

Centisome position: 37.06

GC content: 43.15

Gene sequence:

>693_bases
ATGGATAATAAAATTATTGTTGCTTTAGACTATGAAACAGAATATGAAGCATTGAGTTTTGTAGATCAAGTTGATCCGTC
TCTTTGTCGTGTGAAAGTGGGCAAAGAAATGTTTACGACGTTAGGCACGAATTTTGTTAAACAATTACACGAACGTAAAT
TTGATGTTTTCCTTGATTTAAAATATCACGATATTCCGAATACCGTGGCTCGTGCGGTACGTTCCGCTGCGGATTTAGGC
GTGTGGATGGTGGATTTGCACGCTTCGGGCGGCTTAACCATGATGGAGGAAGCGAAAAAAATCTTAGAGCCGTACGGTAA
AGATGCGCCGTTACTTATTGCGGTAACGGTTTTAACCAGTATGGAAGATTTAGACTTATTACAAATCGGTATTAACGCTT
CGCCGATGGAACAGGTGATTCGTTTGGCGCACCTTGCGCAACGTGCCGGCTTAGACGGTGTGGTTTGTTCTCCGCAAGAA
GTAGAAGTGCTTCGTACGCATTGTGGTAAAGATTTTAAATTAGTGACGCCGGGTATTCGTCCGGAGGGTTCTGATGTCGG
CGATCAACGTCGAATTATGACGCCTAAACAAGCGATTGAAACCGGATCCGATTATTTGGTTATCGGTCGTCCGATTACAC
AGGCTCAAGACCCGTTATCGGTATTAAAATCAATTAACGCCTCTATTCGTTAA

Upstream 100 bases:

>100_bases
AAGTTGGCATTGTCCGTCTTGTCGCCGATGGGAACAAATTAAACCTATACACAGCATTGATTCACAAATTAATTGAATAA
ACTAAAATTGAGGAGTCCTT

Downstream 100 bases:

>100_bases
TTAGTCACGGATTAAATAAGGTGGGCTTTTTTGCTCGCCTTTTTGTTTTAAGCGGTCGAATTTTAAGGATTTTTTACTCA
TGACATTAGTCTATTCAACG

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MDNKIIVALDYETEYEALSFVDQVDPSLCRVKVGKEMFTTLGTNFVKQLHERKFDVFLDLKYHDIPNTVARAVRSAADLG
VWMVDLHASGGLTMMEEAKKILEPYGKDAPLLIAVTVLTSMEDLDLLQIGINASPMEQVIRLAHLAQRAGLDGVVCSPQE
VEVLRTHCGKDFKLVTPGIRPEGSDVGDQRRIMTPKQAIETGSDYLVIGRPITQAQDPLSVLKSINASIR

Sequences:

>Translated_230_residues
MDNKIIVALDYETEYEALSFVDQVDPSLCRVKVGKEMFTTLGTNFVKQLHERKFDVFLDLKYHDIPNTVARAVRSAADLG
VWMVDLHASGGLTMMEEAKKILEPYGKDAPLLIAVTVLTSMEDLDLLQIGINASPMEQVIRLAHLAQRAGLDGVVCSPQE
VEVLRTHCGKDFKLVTPGIRPEGSDVGDQRRIMTPKQAIETGSDYLVIGRPITQAQDPLSVLKSINASIR
>Mature_230_residues
MDNKIIVALDYETEYEALSFVDQVDPSLCRVKVGKEMFTTLGTNFVKQLHERKFDVFLDLKYHDIPNTVARAVRSAADLG
VWMVDLHASGGLTMMEEAKKILEPYGKDAPLLIAVTVLTSMEDLDLLQIGINASPMEQVIRLAHLAQRAGLDGVVCSPQE
VEVLRTHCGKDFKLVTPGIRPEGSDVGDQRRIMTPKQAIETGSDYLVIGRPITQAQDPLSVLKSINASIR

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=229, Percent_Identity=61.5720524017467, Blast_Score=293, Evalue=5e-81,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_ACTP2 (A3N0A0)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053441.1
- ProteinModelPortal:   A3N0A0
- SMR:   A3N0A0
- STRING:   A3N0A0
- GeneID:   4849199
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0736
- eggNOG:   COG0284
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- PhylomeDB:   A3N0A0
- ProtClustDB:   PRK00230
- BioCyc:   APLE416269:APL_0736-MONOMER
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 25419; Mature: 25419

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 61-61 BINDING 10-10 BINDING 32-32 BINDING 119-119 BINDING 180-180 BINDING 189-189 BINDING 209-209 BINDING 210-210

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNKIIVALDYETEYEALSFVDQVDPSLCRVKVGKEMFTTLGTNFVKQLHERKFDVFLDL
CCCCEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
KYHDIPNTVARAVRSAADLGVWMVDLHASGGLTMMEEAKKILEPYGKDAPLLIAVTVLTS
EECCCCHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHC
MEDLDLLQIGINASPMEQVIRLAHLAQRAGLDGVVCSPQEVEVLRTHCGKDFKLVTPGIR
CCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHCCCCCEEECCCCC
PEGSDVGDQRRIMTPKQAIETGSDYLVIGRPITQAQDPLSVLKSINASIR
CCCCCCCCCCCCCCHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MDNKIIVALDYETEYEALSFVDQVDPSLCRVKVGKEMFTTLGTNFVKQLHERKFDVFLDL
CCCCEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
KYHDIPNTVARAVRSAADLGVWMVDLHASGGLTMMEEAKKILEPYGKDAPLLIAVTVLTS
EECCCCHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHC
MEDLDLLQIGINASPMEQVIRLAHLAQRAGLDGVVCSPQEVEVLRTHCGKDFKLVTPGIR
CCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHCCCCCEEECCCCC
PEGSDVGDQRRIMTPKQAIETGSDYLVIGRPITQAQDPLSVLKSINASIR
CCCCCCCCCCCCCCHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA