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The map label for this gene is pmbA [H]

Identifier: 126208209

GI number: 126208209

Start: 837571

End: 838920

Strand: Reverse

Name: pmbA [H]

Synonym: APL_0729

Alternate gene names: 126208209

Gene position: 838920-837571 (Counterclockwise)

Preceding gene: 126208215

Following gene: 126208203

Centisome position: 36.88

GC content: 44.44

Gene sequence:

>1350_bases
ATGTCATTAACAACAAAACAAGATCTCCAAAAGCAAGAACAAGAACTTCGTCAAGCGGTCGAATTTGCACTGAATTTTGC
CAAAAAATCAGGAGCAGAAGCGGAAGTCGGCGTCACTAAAGTTGCCGGACTTTCGGTTTCAACTCGTTTGGAACAAACGG
AAAATATCGAATTTAATAATGACGGCTCACTCGGGATTTCGGTATATGTCGGCAAACGTAAGGGTAATGCTTCGACCTCG
GATTTACAACCGCAATCGATTCAACGAGCGGTCGAATCCGCGTTAGCGATTGCAAAATATACCTCGGAAGACGAGTGTGC
GGGTTTAGCCGATAAAGAGATGATGGCGTTTGAAGCACCGGATCTGGAACTTTATCACCAAGCGGATATTTCGATCGAAC
AAGCGGTTGAATTAGCTTTAGAAGCGGAGCATCACGCCCTGAATGCGGATGAGAAAATTGTGAATAGTGAAGGCGCAACC
TTTAATTCGCATAGCGGTGTGAGAGTGTACGGCAATACTCACGGAATGTTACAAAGCTACCTCTCCAGCCGTTATTCGCT
GTCTTGTAGTGTGATTTCCGCCTATGAAGATCAATTGGAACGTGATTATGAATACACGATTTCACGTGAGTTCGACAAAT
TACAATCGCCGAAATGGATCGGTCGTCAGGCAGCGTTTAAAGCGGTTGATCGTTTGAATCCGCAAAAGATCAAAACCTGT
GAAGTGCCGGTAATTTTTTACAACGATGTAGCAACCGGTTTAATCGGACATTTAGCCGGTGCTATTAGCGGCGGAGCGTT
GTACCGTAAATCGAGTTTCTTATTAGATAAATTAGGCACGCAAATTTTACCAAGTTGGTTTGCAATCTCTGAGCGCCCGC
ATTTATTACGTCAATTAGCTTCATCCGCTTTCGATAGCGAAGGGGTAATTACCCAAGATAGAGAAATTATTACTGACGGT
GTATTGCAAACTTATCTGATGACCAGCTATTCGGCGCGTAAGATGGGCTTAAAAACGACCGGACACGCAGGCGGTATTCA
TAACTGGTTAGTCAAACCGAATCGTACAGGCGGTTTGGATACGCTTCTAAAAGAGATGGGTAGCGGTTTATTGGCGACTG
AATTTTTAGGCTCGGCAATTAATGCGGTAACCGGCGAATATTCGCGCGGTGCGGCAGGTTTTTGGGTGGAAAACGGCGAG
ATTCAGTATCCGGTGGCGGAAATTACCATTGCCGGTCAATTGCAAGAAATGTATAAAAATCTGATTGCAGTGGCGGATGA
TATCGAGCATCGTTCGAATATTCAAACCGGTTCGATTTTATTGGATAAGATGAAAATTTCCGGCGAATAA

Upstream 100 bases:

>100_bases
CCTTCAATCGTAAAAAATACCGTATTCTAACAGAAATTTAAGGTAGAATATACGCATCCTCCTTAGCCATTTTCAATTAT
CAATTTTAAGTTAAAAAATC

Downstream 100 bases:

>100_bases
AAATTTACAATATTGAGAATAATTCTTATTGACATTATTAATAAGATTTCTTAGAATGAACTCAGTTACATTGTTAGGGT
AATGTAACAAATGCTAAATA

Product: antibiotic maturation factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MSLTTKQDLQKQEQELRQAVEFALNFAKKSGAEAEVGVTKVAGLSVSTRLEQTENIEFNNDGSLGISVYVGKRKGNASTS
DLQPQSIQRAVESALAIAKYTSEDECAGLADKEMMAFEAPDLELYHQADISIEQAVELALEAEHHALNADEKIVNSEGAT
FNSHSGVRVYGNTHGMLQSYLSSRYSLSCSVISAYEDQLERDYEYTISREFDKLQSPKWIGRQAAFKAVDRLNPQKIKTC
EVPVIFYNDVATGLIGHLAGAISGGALYRKSSFLLDKLGTQILPSWFAISERPHLLRQLASSAFDSEGVITQDREIITDG
VLQTYLMTSYSARKMGLKTTGHAGGIHNWLVKPNRTGGLDTLLKEMGSGLLATEFLGSAINAVTGEYSRGAAGFWVENGE
IQYPVAEITIAGQLQEMYKNLIAVADDIEHRSNIQTGSILLDKMKISGE

Sequences:

>Translated_449_residues
MSLTTKQDLQKQEQELRQAVEFALNFAKKSGAEAEVGVTKVAGLSVSTRLEQTENIEFNNDGSLGISVYVGKRKGNASTS
DLQPQSIQRAVESALAIAKYTSEDECAGLADKEMMAFEAPDLELYHQADISIEQAVELALEAEHHALNADEKIVNSEGAT
FNSHSGVRVYGNTHGMLQSYLSSRYSLSCSVISAYEDQLERDYEYTISREFDKLQSPKWIGRQAAFKAVDRLNPQKIKTC
EVPVIFYNDVATGLIGHLAGAISGGALYRKSSFLLDKLGTQILPSWFAISERPHLLRQLASSAFDSEGVITQDREIITDG
VLQTYLMTSYSARKMGLKTTGHAGGIHNWLVKPNRTGGLDTLLKEMGSGLLATEFLGSAINAVTGEYSRGAAGFWVENGE
IQYPVAEITIAGQLQEMYKNLIAVADDIEHRSNIQTGSILLDKMKISGE
>Mature_448_residues
SLTTKQDLQKQEQELRQAVEFALNFAKKSGAEAEVGVTKVAGLSVSTRLEQTENIEFNNDGSLGISVYVGKRKGNASTSD
LQPQSIQRAVESALAIAKYTSEDECAGLADKEMMAFEAPDLELYHQADISIEQAVELALEAEHHALNADEKIVNSEGATF
NSHSGVRVYGNTHGMLQSYLSSRYSLSCSVISAYEDQLERDYEYTISREFDKLQSPKWIGRQAAFKAVDRLNPQKIKTCE
VPVIFYNDVATGLIGHLAGAISGGALYRKSSFLLDKLGTQILPSWFAISERPHLLRQLASSAFDSEGVITQDREIITDGV
LQTYLMTSYSARKMGLKTTGHAGGIHNWLVKPNRTGGLDTLLKEMGSGLLATEFLGSAINAVTGEYSRGAAGFWVENGEI
QYPVAEITIAGQLQEMYKNLIAVADDIEHRSNIQTGSILLDKMKISGE

Specific function: May Facilitate The Secretion Of The Peptide Antibiotic Microcin B17 (Mccb17) By Completing Its Maturation. Suppresses The Inhibitory Activity Of The Carbon Storage Regulator (Csra). [C]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=451, Percent_Identity=57.4279379157428, Blast_Score=556, Evalue=1e-160,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 49122; Mature: 48991

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTTKQDLQKQEQELRQAVEFALNFAKKSGAEAEVGVTKVAGLSVSTRLEQTENIEFNN
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEECCCHHHHHHHHCCCCCCC
DGSLGISVYVGKRKGNASTSDLQPQSIQRAVESALAIAKYTSEDECAGLADKEMMAFEAP
CCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHEECCC
DLELYHQADISIEQAVELALEAEHHALNADEKIVNSEGATFNSHSGVRVYGNTHGMLQSY
CCEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCEECCCCCEEEECCCHHHHHHH
LSSRYSLSCSVISAYEDQLERDYEYTISREFDKLQSPKWIGRQAAFKAVDRLNPQKIKTC
HCCCCEEEHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEE
EVPVIFYNDVATGLIGHLAGAISGGALYRKSSFLLDKLGTQILPSWFAISERPHLLRQLA
CCCEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHH
SSAFDSEGVITQDREIITDGVLQTYLMTSYSARKMGLKTTGHAGGIHNWLVKPNRTGGLD
HHCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHH
TLLKEMGSGLLATEFLGSAINAVTGEYSRGAAGFWVENGEIQYPVAEITIAGQLQEMYKN
HHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCEEECCCEEEEEHHHHHHHHHH
LIAVADDIEHRSNIQTGSILLDKMKISGE
HHHHHHHHHHHCCCCCCCEEEEEHCCCCC
>Mature Secondary Structure 
SLTTKQDLQKQEQELRQAVEFALNFAKKSGAEAEVGVTKVAGLSVSTRLEQTENIEFNN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEECCCHHHHHHHHCCCCCCC
DGSLGISVYVGKRKGNASTSDLQPQSIQRAVESALAIAKYTSEDECAGLADKEMMAFEAP
CCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHEECCC
DLELYHQADISIEQAVELALEAEHHALNADEKIVNSEGATFNSHSGVRVYGNTHGMLQSY
CCEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCEECCCCCEEEECCCHHHHHHH
LSSRYSLSCSVISAYEDQLERDYEYTISREFDKLQSPKWIGRQAAFKAVDRLNPQKIKTC
HCCCCEEEHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEE
EVPVIFYNDVATGLIGHLAGAISGGALYRKSSFLLDKLGTQILPSWFAISERPHLLRQLA
CCCEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHH
SSAFDSEGVITQDREIITDGVLQTYLMTSYSARKMGLKTTGHAGGIHNWLVKPNRTGGLD
HHCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHH
TLLKEMGSGLLATEFLGSAINAVTGEYSRGAAGFWVENGEIQYPVAEITIAGQLQEMYKN
HHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCEEECCCEEEEEHHHHHHHHHH
LIAVADDIEHRSNIQTGSILLDKMKISGE
HHHHHHHHHHHCCCCCCCEEEEEHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]