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The map label for this gene is tgt [H]

Identifier: 126208203

GI number: 126208203

Start: 831585

End: 832733

Strand: Reverse

Name: tgt [H]

Synonym: APL_0723

Alternate gene names: 126208203

Gene position: 832733-831585 (Counterclockwise)

Preceding gene: 126208209

Following gene: 126208202

Centisome position: 36.61

GC content: 44.04

Gene sequence:

>1149_bases
ATGAAATATGAATTAAAAACGACCAGCGGCAATGCGCGTCGCGGTCGTTTAACGTTTTCTCGCCCGAAAGGCGAATATGT
GGTTGAAACGCCGGCATTTATGCCGGTCGGTACCTACGGTACGGTAAAAGGAATGACGCCGGAAGAAGTAGCGGCAACCG
GTGCGCAAATTTTATTAGGCAATACTTTCCATCTGTGGCTGCGCCCGGGGCAAGAAGTGATGAAATCACACGGTGATTTA
CATGGCTTTATGCAATGGCACGGTCCGATTTTAACCGATTCGGGCGGTTTCCAAGTATTCAGTTTAGGCAAATTACGTAA
AATCAAAGAAGAAGGGGTAACTTTCCAAAACCCGATTAGCGGTGAGAAAATTTTCCTTTCGCCGGAAAAATCGATGGAAA
TTCAATACGATCTCGGTTCGGATATCGTGATGATCTTTGATGAATGTACGCCGTATCCGGCAACCTTCGATTATGCTAAA
AACTCAATGGAAATGTCATTACGTTGGGCGAAACGTAGTCGAGATCGTTTTGACGAGCTACAAAATCCGCGTGCGTTATT
CGGAATCGTACAGGGCGGGACTTACGAAGAGTTGCGTAAGATCTCGGTAGAAGGTTTGGTCAATATCGGTTTTGACGGTT
ATGCGGTCGGCGGTTTGGCGGTAGGCGAACCGAAAGAAGAAATGCACCGTATCCTTGAATTTACCACACCGTTATTACCG
CAAGATAAACCTCGTTATTTAATGGGCGTAGGTAAACCGGAAGACTTAGTGGAAGGTGTGCGTCGCGGTATCGATATGTT
CGATTGCGTAATGCCGACCCGTAATGCACGTAACGGACATTTATTTGTCAGCAACGGTATCGTGAAAATTCGTAATGCGA
AATATAAAACGGATACCACACCGTTAGATCCGGAATGCGATTGCTACACTTGTAAAAACTATACGAAAGCCTATTTATAT
CACTTAGATAAATGCGGTGAGATTTTAGGCGCGCGTTTAAATACGATTCATAACTTACGCTATTATCAACGCTTAATGGC
ACAGATTCGCCAAGCGATTGAAGAAGATCGTTTTGATGATTTTGTGGTTGAATTTTATGCCAAAATCGGTAAAGAAGTTC
CGCCGTTACAATCGGAAGTGAATAAATAA

Upstream 100 bases:

>100_bases
TCAGTTTAGGAACCGGTATTCGCTTTTAATTTGTAAATAATAAGTGGAATATGACCGCTTGTTAAGCTGTTTATAGACTG
TTTATCTATAAGGAATTTGA

Downstream 100 bases:

>100_bases
TTCCAAATTTTTTATGGCACAAGTTTAGGCTTGTGCCTTTTTATCAAAGGGTGATTAGATAGAGAGGTGATTATGCAACA
ACTTGAACCGCAACAATTCG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MKYELKTTSGNARRGRLTFSRPKGEYVVETPAFMPVGTYGTVKGMTPEEVAATGAQILLGNTFHLWLRPGQEVMKSHGDL
HGFMQWHGPILTDSGGFQVFSLGKLRKIKEEGVTFQNPISGEKIFLSPEKSMEIQYDLGSDIVMIFDECTPYPATFDYAK
NSMEMSLRWAKRSRDRFDELQNPRALFGIVQGGTYEELRKISVEGLVNIGFDGYAVGGLAVGEPKEEMHRILEFTTPLLP
QDKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVSNGIVKIRNAKYKTDTTPLDPECDCYTCKNYTKAYLY
HLDKCGEILGARLNTIHNLRYYQRLMAQIRQAIEEDRFDDFVVEFYAKIGKEVPPLQSEVNK

Sequences:

>Translated_382_residues
MKYELKTTSGNARRGRLTFSRPKGEYVVETPAFMPVGTYGTVKGMTPEEVAATGAQILLGNTFHLWLRPGQEVMKSHGDL
HGFMQWHGPILTDSGGFQVFSLGKLRKIKEEGVTFQNPISGEKIFLSPEKSMEIQYDLGSDIVMIFDECTPYPATFDYAK
NSMEMSLRWAKRSRDRFDELQNPRALFGIVQGGTYEELRKISVEGLVNIGFDGYAVGGLAVGEPKEEMHRILEFTTPLLP
QDKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVSNGIVKIRNAKYKTDTTPLDPECDCYTCKNYTKAYLY
HLDKCGEILGARLNTIHNLRYYQRLMAQIRQAIEEDRFDDFVVEFYAKIGKEVPPLQSEVNK
>Mature_382_residues
MKYELKTTSGNARRGRLTFSRPKGEYVVETPAFMPVGTYGTVKGMTPEEVAATGAQILLGNTFHLWLRPGQEVMKSHGDL
HGFMQWHGPILTDSGGFQVFSLGKLRKIKEEGVTFQNPISGEKIFLSPEKSMEIQYDLGSDIVMIFDECTPYPATFDYAK
NSMEMSLRWAKRSRDRFDELQNPRALFGIVQGGTYEELRKISVEGLVNIGFDGYAVGGLAVGEPKEEMHRILEFTTPLLP
QDKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVSNGIVKIRNAKYKTDTTPLDPECDCYTCKNYTKAYLY
HLDKCGEILGARLNTIHNLRYYQRLMAQIRQAIEEDRFDDFVVEFYAKIGKEVPPLQSEVNK

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=372, Percent_Identity=43.5483870967742, Blast_Score=304, Evalue=9e-83,
Organism=Escherichia coli, GI1786607, Length=379, Percent_Identity=79.155672823219, Blast_Score=638, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17544680, Length=382, Percent_Identity=42.6701570680628, Blast_Score=301, Evalue=3e-82,
Organism=Caenorhabditis elegans, GI25146502, Length=190, Percent_Identity=33.1578947368421, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19920510, Length=373, Percent_Identity=44.5040214477212, Blast_Score=316, Evalue=2e-86,
Organism=Drosophila melanogaster, GI21355311, Length=156, Percent_Identity=32.0512820512821, Blast_Score=85, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 43359; Mature: 43359

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYELKTTSGNARRGRLTFSRPKGEYVVETPAFMPVGTYGTVKGMTPEEVAATGAQILLG
CCEEEECCCCCCCCCCEEEECCCCCEEEECCCCEECCCCCCCCCCCHHHHHHCCCEEEEC
NTFHLWLRPGQEVMKSHGDLHGFMQWHGPILTDSGGFQVFSLGKLRKIKEEGVTFQNPIS
CEEEEEECCCHHHHHHCCCCEEHHHHCCCEEECCCCEEEEECHHHHHHHHCCCEECCCCC
GEKIFLSPEKSMEIQYDLGSDIVMIFDECTPYPATFDYAKNSMEMSLRWAKRSRDRFDEL
CCEEEECCCCCEEEEEECCCCEEEEEECCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHH
QNPRALFGIVQGGTYEELRKISVEGLVNIGFDGYAVGGLAVGEPKEEMHRILEFTTPLLP
CCCCEEEEEECCCCHHHHHHHHHHHEEEECCCCEEECCEECCCCHHHHHHHHHHHCCCCC
QDKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVSNGIVKIRNAKYKTDTT
CCCCCEEEECCCHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEECCEEEEECCEEECCCC
PLDPECDCYTCKNYTKAYLYHLDKCGEILGARLNTIHNLRYYQRLMAQIRQAIEEDRFDD
CCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
FVVEFYAKIGKEVPPLQSEVNK
HHHHHHHHHCCCCCCHHHHCCC
>Mature Secondary Structure
MKYELKTTSGNARRGRLTFSRPKGEYVVETPAFMPVGTYGTVKGMTPEEVAATGAQILLG
CCEEEECCCCCCCCCCEEEECCCCCEEEECCCCEECCCCCCCCCCCHHHHHHCCCEEEEC
NTFHLWLRPGQEVMKSHGDLHGFMQWHGPILTDSGGFQVFSLGKLRKIKEEGVTFQNPIS
CEEEEEECCCHHHHHHCCCCEEHHHHCCCEEECCCCEEEEECHHHHHHHHCCCEECCCCC
GEKIFLSPEKSMEIQYDLGSDIVMIFDECTPYPATFDYAKNSMEMSLRWAKRSRDRFDEL
CCEEEECCCCCEEEEEECCCCEEEEEECCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHH
QNPRALFGIVQGGTYEELRKISVEGLVNIGFDGYAVGGLAVGEPKEEMHRILEFTTPLLP
CCCCEEEEEECCCCHHHHHHHHHHHEEEECCCCEEECCEECCCCHHHHHHHHHHHCCCCC
QDKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVSNGIVKIRNAKYKTDTT
CCCCCEEEECCCHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEECCEEEEECCEEECCCC
PLDPECDCYTCKNYTKAYLYHLDKCGEILGARLNTIHNLRYYQRLMAQIRQAIEEDRFDD
CCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
FVVEFYAKIGKEVPPLQSEVNK
HHHHHHHHHCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA