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The map label for this gene is cspD [H]

Identifier: 126208205

GI number: 126208205

Start: 833751

End: 833942

Strand: Direct

Name: cspD [H]

Synonym: APL_0725

Alternate gene names: 126208205

Gene position: 833751-833942 (Clockwise)

Preceding gene: 126208204

Following gene: 126208206

Centisome position: 36.66

GC content: 39.06

Gene sequence:

>192_bases
GTGAAATGGTTCAATAGTGCGAAAGGATTCGGATTTATTACAAGCGATAATGTCGAAGGCGATATTTTTGCGCATTTTTC
CGAAATTCAGAGCGAAGGATATCGTTCGCTTAAAGTAGGTCAAAAGGTACAATTTGAGCTGATTAACGGTGAACGCGGTG
CATCAGCCGCTAAAATTTCATTAGTTGAATAA

Upstream 100 bases:

>100_bases
ATTTTCAAACATTTCCGATTGTGAGGTTCACTTTTTTGACTACAATGCACCGCGTAATATCAATGCCGTAAGATAGGAGA
GAATTACGGAAGTTGGCATC

Downstream 100 bases:

>100_bases
TTCAAAACCCGATTTACAAGCGGTCGAATTTGTATAATTTTTACAAATTCGACCGCTTTTTTATTCTATGCTAGAATGCA
GAAAGATTTAGTAACAAATT

Product: cold shock-like protein CspD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 63; Mature: 63

Protein sequence:

>63_residues
MKWFNSAKGFGFITSDNVEGDIFAHFSEIQSEGYRSLKVGQKVQFELINGERGASAAKISLVE

Sequences:

>Translated_63_residues
MKWFNSAKGFGFITSDNVEGDIFAHFSEIQSEGYRSLKVGQKVQFELINGERGASAAKISLVE
>Mature_63_residues
MKWFNSAKGFGFITSDNVEGDIFAHFSEIQSEGYRSLKVGQKVQFELINGERGASAAKISLVE

Specific function: Inhibits DNA Replication At Both Initiation And Elongation Steps, Most Probably By Binding To The Opened, Single- Stranded Regions At Replication Forks. Plays A Regulatory Role In Chromosomal Replication In Nutrient-Depleted Cells. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=63, Percent_Identity=55.5555555555556, Blast_Score=75, Evalue=1e-15,
Organism=Escherichia coli, GI1786841, Length=59, Percent_Identity=52.5423728813559, Blast_Score=75, Evalue=1e-15,
Organism=Escherichia coli, GI1789979, Length=60, Percent_Identity=51.6666666666667, Blast_Score=72, Evalue=5e-15,
Organism=Escherichia coli, GI2367114, Length=59, Percent_Identity=50.8474576271186, Blast_Score=70, Evalue=3e-14,
Organism=Escherichia coli, GI1788126, Length=62, Percent_Identity=45.1612903225806, Blast_Score=69, Evalue=8e-14,
Organism=Escherichia coli, GI1787839, Length=63, Percent_Identity=46.031746031746, Blast_Score=66, Evalue=4e-13,
Organism=Escherichia coli, GI1787834, Length=59, Percent_Identity=45.7627118644068, Blast_Score=63, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012751
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 6971; Mature: 6971

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKWFNSAKGFGFITSDNVEGDIFAHFSEIQSEGYRSLKVGQKVQFELINGERGASAAKIS
CCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCEECCCCEEEEEEECCCCCCCEEEEE
LVE
EEC
>Mature Secondary Structure
MKWFNSAKGFGFITSDNVEGDIFAHFSEIQSEGYRSLKVGQKVQFELINGERGASAAKIS
CCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCEECCCCEEEEEEECCCCCCCEEEEE
LVE
EEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]