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The map label for this gene is pitA [C]

Identifier: 126208199

GI number: 126208199

Start: 828778

End: 830040

Strand: Direct

Name: pitA [C]

Synonym: APL_0719

Alternate gene names: 126208199

Gene position: 828778-830040 (Clockwise)

Preceding gene: 126208198

Following gene: 126208200

Centisome position: 36.44

GC content: 43.86

Gene sequence:

>1263_bases
ATGGAACTCATTCAGCAATATGGCAATCTTTTAGTCATTATTACTGCACTTTTTGGTTTTTTTATGGCTTTCGGTATCGG
AGCGAACGATGTATCTAACGCAATGGGTACATCTGTTGGTTCAGGTACGGTTACAGCTCGCCAAGCAATCGTTATTGCGA
TGGTATTTGAATTTGCCGGTGCTTACCTTGCAGGTGGTGAAGTAACCGAAACAATTAAAAGCGGTATTATCGATGTTTCA
CTTTTTGCAGATAAGCCGGATATTCTTGTCTTAGGTATGATGTCGGCACTATTTGCCGCCGGTTTCTGGCTATTAGTTGC
ATCGAAAATGGGATGGCCGGTTTCAACAACACACGCGATTATCGGGGCAATTATTGGTTTCGGCTGTTTAACTGTCGGTA
GCGAAGCCGTACAGTGGAAACAATTGGGTGGGATTGTTGGTAGCTGGTTTATTACACCTGTTATCGCCGGTTTTGTCGCT
TATTGGATTTTTATTAGTACACAAAAACTTATTTTCGATACCGATGAACCGATGAAAAATGCGCAAAAGTACGGTCCGTT
CTATATGGCACTTACCGCTTTTATTTTAAGTATCGTGACTATGACTAAGGGCTTAAAACATGTCGGTTTGCATTTAACCA
CAACGGAAACCGTACTTATTTCCTCAGCCATCGCAGCCGTCGCCGTAGTAACTTGTTACTTCTATTTCCGTAGCGAAGCA
TTTGCAAAACGCGCTCAAGGTGCTGCATTTGGTGGTGTAGAAAAAGTATTCAGTATTTTAATGCTTTTAACCGCTTGTTC
AATGGCATTCGCACACGGCTCGAACGATGTGGCAAATGCGGTCGGTCCGTTATCCGCAGTGGTATCTATCGTGCATAGCG
GCGGTGTTGTGGAAGGGAAAGCGGCATTGGCTTCATGGATTCTACCGTTAGGTGCGGCGGGTATCGCAGCCGGTATGCTT
ATCATGGGCTATAAAGTAATGGGAACGATGGGTACCGGTATTACGGATTTAACGCCAAGCCGCGGTTTCTCTGCCGAATT
TGCTTGTGCGACTACCGTAGTAATCGCTTCAGGTACCGGTTTACCTATTTCAACGACACAAACCATTGTAGGGGCAATCT
TAGGTGTCGGATTTGCTCGCGGTATTGCAGCCTTAAACTTAGGTATTATTCGTAATATTGTTGCCTCATGGGTCGTCACA
TTACCGGCAGGCGCAATCATTGCAATTGTTATCTATGAAATCTTATTAATGATTTTTTATTAA

Upstream 100 bases:

>100_bases
TTTATATAAATGTATTGAACGAGTGAGCATTTTAGCTGATCAAGCTCAACGTGTCGGTTCACGAATCGAGCTCATGTTAG
CTAAAGCGTAGGAGATTAGA

Downstream 100 bases:

>100_bases
TTTTATCGTTCGGGGAAAGTATGTTTAAACATATCTTTCCCTTTCATTTAAAAGGACTCGATTATGTTAAAACGGAAGTT
AATCCTAATTGCTTATACCT

Product: putative phosphate permease

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MELIQQYGNLLVIITALFGFFMAFGIGANDVSNAMGTSVGSGTVTARQAIVIAMVFEFAGAYLAGGEVTETIKSGIIDVS
LFADKPDILVLGMMSALFAAGFWLLVASKMGWPVSTTHAIIGAIIGFGCLTVGSEAVQWKQLGGIVGSWFITPVIAGFVA
YWIFISTQKLIFDTDEPMKNAQKYGPFYMALTAFILSIVTMTKGLKHVGLHLTTTETVLISSAIAAVAVVTCYFYFRSEA
FAKRAQGAAFGGVEKVFSILMLLTACSMAFAHGSNDVANAVGPLSAVVSIVHSGGVVEGKAALASWILPLGAAGIAAGML
IMGYKVMGTMGTGITDLTPSRGFSAEFACATTVVIASGTGLPISTTQTIVGAILGVGFARGIAALNLGIIRNIVASWVVT
LPAGAIIAIVIYEILLMIFY

Sequences:

>Translated_420_residues
MELIQQYGNLLVIITALFGFFMAFGIGANDVSNAMGTSVGSGTVTARQAIVIAMVFEFAGAYLAGGEVTETIKSGIIDVS
LFADKPDILVLGMMSALFAAGFWLLVASKMGWPVSTTHAIIGAIIGFGCLTVGSEAVQWKQLGGIVGSWFITPVIAGFVA
YWIFISTQKLIFDTDEPMKNAQKYGPFYMALTAFILSIVTMTKGLKHVGLHLTTTETVLISSAIAAVAVVTCYFYFRSEA
FAKRAQGAAFGGVEKVFSILMLLTACSMAFAHGSNDVANAVGPLSAVVSIVHSGGVVEGKAALASWILPLGAAGIAAGML
IMGYKVMGTMGTGITDLTPSRGFSAEFACATTVVIASGTGLPISTTQTIVGAILGVGFARGIAALNLGIIRNIVASWVVT
LPAGAIIAIVIYEILLMIFY
>Mature_420_residues
MELIQQYGNLLVIITALFGFFMAFGIGANDVSNAMGTSVGSGTVTARQAIVIAMVFEFAGAYLAGGEVTETIKSGIIDVS
LFADKPDILVLGMMSALFAAGFWLLVASKMGWPVSTTHAIIGAIIGFGCLTVGSEAVQWKQLGGIVGSWFITPVIAGFVA
YWIFISTQKLIFDTDEPMKNAQKYGPFYMALTAFILSIVTMTKGLKHVGLHLTTTETVLISSAIAAVAVVTCYFYFRSEA
FAKRAQGAAFGGVEKVFSILMLLTACSMAFAHGSNDVANAVGPLSAVVSIVHSGGVVEGKAALASWILPLGAAGIAAGML
IMGYKVMGTMGTGITDLTPSRGFSAEFACATTVVIASGTGLPISTTQTIVGAILGVGFARGIAALNLGIIRNIVASWVVT
LPAGAIIAIVIYEILLMIFY

Specific function: Potential transporter for phosphate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family [H]

Homologues:

Organism=Homo sapiens, GI5803173, Length=215, Percent_Identity=40, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI31543630, Length=290, Percent_Identity=33.448275862069, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI25146401, Length=456, Percent_Identity=33.1140350877193, Blast_Score=232, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI71981576, Length=439, Percent_Identity=33.7129840546697, Blast_Score=228, Evalue=6e-60,
Organism=Caenorhabditis elegans, GI32566716, Length=468, Percent_Identity=31.8376068376068, Blast_Score=218, Evalue=6e-57,
Organism=Caenorhabditis elegans, GI17539280, Length=498, Percent_Identity=28.5140562248996, Blast_Score=202, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17557328, Length=494, Percent_Identity=25.3036437246964, Blast_Score=147, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI17536725, Length=160, Percent_Identity=33.75, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6319773, Length=183, Percent_Identity=37.1584699453552, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI21356511, Length=196, Percent_Identity=42.8571428571429, Blast_Score=166, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 43671; Mature: 43671

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELIQQYGNLLVIITALFGFFMAFGIGANDVSNAMGTSVGSGTVTARQAIVIAMVFEFAG
CCHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHC
AYLAGGEVTETIKSGIIDVSLFADKPDILVLGMMSALFAAGFWLLVASKMGWPVSTTHAI
CHHCCCHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
IGAIIGFGCLTVGSEAVQWKQLGGIVGSWFITPVIAGFVAYWIFISTQKLIFDTDEPMKN
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEECCCCHHHH
AQKYGPFYMALTAFILSIVTMTKGLKHVGLHLTTTETVLISSAIAAVAVVTCYFYFRSEA
HHHHCHHHHHHHHHHHHHHHHHHCHHHHCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
FAKRAQGAAFGGVEKVFSILMLLTACSMAFAHGSNDVANAVGPLSAVVSIVHSGGVVEGK
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHH
AALASWILPLGAAGIAAGMLIMGYKVMGTMGTGITDLTPSRGFSAEFACATTVVIASGTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHEEEEECCCC
LPISTTQTIVGAILGVGFARGIAALNLGIIRNIVASWVVTLPAGAIIAIVIYEILLMIFY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MELIQQYGNLLVIITALFGFFMAFGIGANDVSNAMGTSVGSGTVTARQAIVIAMVFEFAG
CCHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHC
AYLAGGEVTETIKSGIIDVSLFADKPDILVLGMMSALFAAGFWLLVASKMGWPVSTTHAI
CHHCCCHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
IGAIIGFGCLTVGSEAVQWKQLGGIVGSWFITPVIAGFVAYWIFISTQKLIFDTDEPMKN
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEECCCCHHHH
AQKYGPFYMALTAFILSIVTMTKGLKHVGLHLTTTETVLISSAIAAVAVVTCYFYFRSEA
HHHHCHHHHHHHHHHHHHHHHHHCHHHHCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
FAKRAQGAAFGGVEKVFSILMLLTACSMAFAHGSNDVANAVGPLSAVVSIVHSGGVVEGK
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHH
AALASWILPLGAAGIAAGMLIMGYKVMGTMGTGITDLTPSRGFSAEFACATTVVIASGTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHEEEEECCCC
LPISTTQTIVGAILGVGFARGIAALNLGIIRNIVASWVVTLPAGAIIAIVIYEILLMIFY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]