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The map label for this gene is ppx [H]

Identifier: 126208188

GI number: 126208188

Start: 817862

End: 819370

Strand: Reverse

Name: ppx [H]

Synonym: APL_0708

Alternate gene names: 126208188

Gene position: 819370-817862 (Counterclockwise)

Preceding gene: 126208191

Following gene: 126208187

Centisome position: 36.02

GC content: 43.01

Gene sequence:

>1509_bases
ATGTCGTCGTCTAAACAATTAATGCCGAGAGAGTTTGCTGCGATCGATTTGGGATCGAACAGCTTTCATATGATTGTGGC
ACGCATTGTAAACGGTTCAATCCAAATACTTTCACGCTTGAAGCGTCGAGTTCGCTTGGCGGACGGTTTAGATGAAAATC
GTATTTTGAGTCAAGAAGCGATTACAAGAGGCGTAGAATGTTTAGCGTTATTTGCCGATCGTTTGCAAGGATTTTCTGCG
GAAAACGTGAAGGTGGTCGGGACTTATACTTTGCGTCGGGCGATTAATAATGAAGACTTTTTAACACAAGCGGCTGAAGT
ATTTCCGTTCCCTATTCAAATCATTTCGGGGCAAGAAGAAGCGCGTTTAATTTATTCCGGAGTCAGTCATACTCAACCTG
AACGCGGACGAAAGTTTGTGGTGGATATCGGCGGCGGTTCGACGGAAATGACGATTGGCGATAATTTTACGCCGTTACGT
GCCGAGAGCCGTCATATGGGATGCGTGAGCTTTGCCAAACGATTTTTTGCAAAGGGCGAGTTAAACATTGAACGTTTTGA
CAGCGCTTATCAACTTGCAATGGAAAAGATCGAAGATTTGGCTTGGGAATATCGTCAGCTCGGTTGGCAACACGTTTTAG
GTTCGTCAGGTACGATAAAAACCGTACAAAAAGTTTTAATTGCGAACGGTTACCGTGACGGATTAATTACGGAAGAACGT
TTACAGGCTTTAATCGACGACTGTTTACGTTTTAATTCGTTAAATAGCATCCAGATGAAGGGGTTACTTGAGGAACGAGC
CGACGTTTTAGTTCCCGGATTAGCTATTTTACTCGCGCTTTTCCATACTTTCAGAATCGAATCGATGCGTTATTCCGACG
GAGCTTTGCGAGAAGGCGTGATGTACGGGTTAGAGAAAAGTTTCCAAGTCGGCGATATTAGACAGCGTACTGCAGAAGCA
TTGGCGGAGCAGTTTAATATCGATCAGCAACAGGCGAATCGAGTGGAACATACGGCATTGGAATTGTTCGAGCAAGTGAA
AAGTTGGCGTAACCGTCGCCAAACGGCGGAATTGTCGAGTATGCTGAAATGGGCGAGTTTGCTGCATGAAATCGGGATCG
TCATCAATCATAACGGCGTTAATAGACATTCCGCTTATATTATTGCGAATCGAGACTTACCCGGATTCGATTTTGAACAG
CAGAATTTATTGGCAACCTTAATGCGCTTTCATCTTAAAGGTTTCAAACGTAGCGATATTCGTAGTACCAATCGTTATCA
GCATCGCGATATTCTTACCTTACTAAGATTATTCAGATTGGCCGTTTTACTTAATCGTTCTCGTCAAGCGACGGTAACTT
CTAAAATCTTAAACTTGCATATTGATAATAACGAATGGTTTCTCTCGTTCGATTCGGGTTTTTTAGCTAAAAATCCTCTC
GTTCTGGCAGACTTGGAAGAAGAAAAAAAATATTTGTCTTCGCTAGGCTTAGTACTCAATGTGGGTTAG

Upstream 100 bases:

>100_bases
ACGATACCATCAAATTAATGAAGAAATTTTCTATCGATTCGTAGTAAAAATGATAGAATCTTAATATTTATCTTCATCGT
TTGTTTATAAGGATATTTTT

Downstream 100 bases:

>100_bases
TCCTGTCAAAGAGGCATAAATATTTATGCCTCGCGTTTTATAAAATTTTGATCTAGTTCTCATTTTTCCCTTTTCTTCCT
TGTTATTTCTAAGATAAGTC

Product: exopolyphosphatase

Products: NA

Alternate protein names: ExopolyPase; Metaphosphatase [H]

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MSSSKQLMPREFAAIDLGSNSFHMIVARIVNGSIQILSRLKRRVRLADGLDENRILSQEAITRGVECLALFADRLQGFSA
ENVKVVGTYTLRRAINNEDFLTQAAEVFPFPIQIISGQEEARLIYSGVSHTQPERGRKFVVDIGGGSTEMTIGDNFTPLR
AESRHMGCVSFAKRFFAKGELNIERFDSAYQLAMEKIEDLAWEYRQLGWQHVLGSSGTIKTVQKVLIANGYRDGLITEER
LQALIDDCLRFNSLNSIQMKGLLEERADVLVPGLAILLALFHTFRIESMRYSDGALREGVMYGLEKSFQVGDIRQRTAEA
LAEQFNIDQQQANRVEHTALELFEQVKSWRNRRQTAELSSMLKWASLLHEIGIVINHNGVNRHSAYIIANRDLPGFDFEQ
QNLLATLMRFHLKGFKRSDIRSTNRYQHRDILTLLRLFRLAVLLNRSRQATVTSKILNLHIDNNEWFLSFDSGFLAKNPL
VLADLEEEKKYLSSLGLVLNVG

Sequences:

>Translated_502_residues
MSSSKQLMPREFAAIDLGSNSFHMIVARIVNGSIQILSRLKRRVRLADGLDENRILSQEAITRGVECLALFADRLQGFSA
ENVKVVGTYTLRRAINNEDFLTQAAEVFPFPIQIISGQEEARLIYSGVSHTQPERGRKFVVDIGGGSTEMTIGDNFTPLR
AESRHMGCVSFAKRFFAKGELNIERFDSAYQLAMEKIEDLAWEYRQLGWQHVLGSSGTIKTVQKVLIANGYRDGLITEER
LQALIDDCLRFNSLNSIQMKGLLEERADVLVPGLAILLALFHTFRIESMRYSDGALREGVMYGLEKSFQVGDIRQRTAEA
LAEQFNIDQQQANRVEHTALELFEQVKSWRNRRQTAELSSMLKWASLLHEIGIVINHNGVNRHSAYIIANRDLPGFDFEQ
QNLLATLMRFHLKGFKRSDIRSTNRYQHRDILTLLRLFRLAVLLNRSRQATVTSKILNLHIDNNEWFLSFDSGFLAKNPL
VLADLEEEKKYLSSLGLVLNVG
>Mature_501_residues
SSSKQLMPREFAAIDLGSNSFHMIVARIVNGSIQILSRLKRRVRLADGLDENRILSQEAITRGVECLALFADRLQGFSAE
NVKVVGTYTLRRAINNEDFLTQAAEVFPFPIQIISGQEEARLIYSGVSHTQPERGRKFVVDIGGGSTEMTIGDNFTPLRA
ESRHMGCVSFAKRFFAKGELNIERFDSAYQLAMEKIEDLAWEYRQLGWQHVLGSSGTIKTVQKVLIANGYRDGLITEERL
QALIDDCLRFNSLNSIQMKGLLEERADVLVPGLAILLALFHTFRIESMRYSDGALREGVMYGLEKSFQVGDIRQRTAEAL
AEQFNIDQQQANRVEHTALELFEQVKSWRNRRQTAELSSMLKWASLLHEIGIVINHNGVNRHSAYIIANRDLPGFDFEQQ
NLLATLMRFHLKGFKRSDIRSTNRYQHRDILTLLRLFRLAVLLNRSRQATVTSKILNLHIDNNEWFLSFDSGFLAKNPLV
LADLEEEKKYLSSLGLVLNVG

Specific function: Degradation of inorganic polyphosphates. Orthophosphate is released progressively from the ends of polyphosphate of circa 500 residues long, while chains of circa 15 residues compete poorly with polyphosphate as substrate [H]

COG id: COG0248

COG function: function code FP; Exopolyphosphatase

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gppA/ppx family [H]

Homologues:

Organism=Escherichia coli, GI1788848, Length=487, Percent_Identity=51.9507186858316, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI48994966, Length=491, Percent_Identity=40.5295315682281, Blast_Score=340, Evalue=2e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022371
- InterPro:   IPR003695 [H]

Pfam domain/function: PF02541 Ppx-GppA [H]

EC number: =3.6.1.11 [H]

Molecular weight: Translated: 57081; Mature: 56950

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSKQLMPREFAAIDLGSNSFHMIVARIVNGSIQILSRLKRRVRLADGLDENRILSQEA
CCCCCCCCCCHHEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHH
ITRGVECLALFADRLQGFSAENVKVVGTYTLRRAINNEDFLTQAAEVFPFPIQIISGQEE
HHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHCCCHHHHHHHHHHCCCCEEEECCCHH
ARLIYSGVSHTQPERGRKFVVDIGGGSTEMTIGDNFTPLRAESRHMGCVSFAKRFFAKGE
HHHHCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCC
LNIERFDSAYQLAMEKIEDLAWEYRQLGWQHVLGSSGTIKTVQKVLIANGYRDGLITEER
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHH
LQALIDDCLRFNSLNSIQMKGLLEERADVLVPGLAILLALFHTFRIESMRYSDGALREGV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHH
MYGLEKSFQVGDIRQRTAEALAEQFNIDQQQANRVEHTALELFEQVKSWRNRRQTAELSS
HHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLKWASLLHEIGIVINHNGVNRHSAYIIANRDLPGFDFEQQNLLATLMRFHLKGFKRSDI
HHHHHHHHHHCCEEEECCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
RSTNRYQHRDILTLLRLFRLAVLLNRSRQATVTSKILNLHIDNNEWFLSFDSGFLAKNPL
CCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEEEEEEECCCEEEEEECCCCCCCCCE
VLADLEEEKKYLSSLGLVLNVG
EEEECHHHHHHHHHCCCEEECC
>Mature Secondary Structure 
SSSKQLMPREFAAIDLGSNSFHMIVARIVNGSIQILSRLKRRVRLADGLDENRILSQEA
CCCCCCCCCHHEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHH
ITRGVECLALFADRLQGFSAENVKVVGTYTLRRAINNEDFLTQAAEVFPFPIQIISGQEE
HHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHCCCHHHHHHHHHHCCCCEEEECCCHH
ARLIYSGVSHTQPERGRKFVVDIGGGSTEMTIGDNFTPLRAESRHMGCVSFAKRFFAKGE
HHHHCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCC
LNIERFDSAYQLAMEKIEDLAWEYRQLGWQHVLGSSGTIKTVQKVLIANGYRDGLITEER
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHH
LQALIDDCLRFNSLNSIQMKGLLEERADVLVPGLAILLALFHTFRIESMRYSDGALREGV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHH
MYGLEKSFQVGDIRQRTAEALAEQFNIDQQQANRVEHTALELFEQVKSWRNRRQTAELSS
HHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLKWASLLHEIGIVINHNGVNRHSAYIIANRDLPGFDFEQQNLLATLMRFHLKGFKRSDI
HHHHHHHHHHCCEEEECCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
RSTNRYQHRDILTLLRLFRLAVLLNRSRQATVTSKILNLHIDNNEWFLSFDSGFLAKNPL
CCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEEEEEEECCCEEEEEECCCCCCCCCE
VLADLEEEKKYLSSLGLVLNVG
EEEECHHHHHHHHHCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]