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The map label for this gene is kpnIM [H]

Identifier: 126208185

GI number: 126208185

Start: 813144

End: 816032

Strand: Reverse

Name: kpnIM [H]

Synonym: APL_0705

Alternate gene names: 126208185

Gene position: 816032-813144 (Counterclockwise)

Preceding gene: 126208186

Following gene: 126208184

Centisome position: 35.88

GC content: 36.24

Gene sequence:

>2889_bases
TTGCAAGAAATTTTTCAAATCAATCAGCCGGATTTAGATTTCGGTATTTATCGCATTCTCAATTCTCGCCAAAACGAAAT
TCAGCAGTTCTTAACTCGCACCTTGCCAAACAACATCAATGGTGCATTTAACGGCAATTTTGAACAGGCAAACAGCGTTT
ACAATCATCTCTACACTTTCTTTTCACGCTATTACGATCAAGGCGATTTTATCAGCCAACGCCGCTACAAAGGCGATACC
TACGCTATTCCTTACGCAGGTGAAGAAGTAATGCTCCACTGGGCGAACAAAGATCAGTATTACACCAAATCGGGCGAAAA
TTTCAGTAATTATGCGTTTACGCTAGAAAATGGCAAGAAAGTGCAATTTCGCCTTGTGGAAGCCGACACCGCAAAAGACA
ACCGCAAAGATAACGACCAGACCCGAGTGTTTGCGTTGATTGAGCCACGCATTCAAACCGCCACAGATGAAAACGGCGAT
GAAATCCAAACCGATATTTTGCCATTTGATATTCAAGGCGATTTACTCACCATTCGTTTTGAATATAAAGCGGTAAACAA
AAAAGAAAAACAGGCGGATTACATCACGCAAGCGGTGGAAAAAATAAAAACTTTTGCCATTAGTGATGAATTTTTGGATA
TTTTAAAACCAATGCCAACAGAGAAAAACAAAACTCACACCTTGTTGGAAAAATATCTCACTGATTACACCGCTAAAAAT
ACCGCTGATTATTTTATTCACAAAAATCTCGGCAAGTTTTTAAATCAAGAGCTTGATTTTTATATCAAAAATGAAGTGAT
GAACTTGGATAACATTCAAGACAGCACTGATTTTTCTCATATTGAGCAAAACTTACAAACCATTAAAACCTTAAAAACGG
TGGCAAAAGAAATTATCGCCTTTTTAGCCCAGTTAGAAGATTTTCAAAAGAAATTATGGCTGAAAAAGAAATTTGTCGCA
GGTTGTCATTATCTGATTACGCTTGATCACTTAACCGAAGAACAAGTGCAACAAGCCTTAGATAATCCAAAACAAACCGC
CCAATGGAAAGCCCTATTTAACGTAGAGACAAGCGGTCAAAATGTGGCAGAAATTTGCAAAAATTATCCGAACTTGGTGG
TGGATACATCTTTATTTGAACCGAAATTTCAAGCTGAAGTATTAAACGAGCTACACGATTTAGACGAGAAAACTAACGGC
TTGCTAATTCATTCTGATAATTTTCAAGCCCTGAATTTATTGCAGGAAAAATATAAAGAGCAAGTGAAATGTATTTATAT
TGATCCGCCGTATAACACGAATGCGTCAGAAATTATTTATAAAAATGGGTATAAGCATTCTAGTTGGAATAGCTTAATTG
AAAATAGAGTTTCTTTATCAAGAAAGTTATTATCAAATGAAGGTTTTTTTGCAGGAACGATAGATTATGCGGAATTATTT
AGTTTCGGAAAAATTTTAGATGAAGAATTTGGCGAATTATCAAGAGTGGGTATTGTGTCAATTTTAATAAATCCCAAAGG
TCGACAACACGAAAGATTTTTCTCTGCATCATCTGAATATATGATTGTTTATACTCCTTCACCAAATTCAGCTAGATTTA
ACCAAACAACTATTGATGCAGAAAAGCGTAAGACATTTAATTTATTTGATGAGGATAAAAAAGAAAGATATAGATTAGAT
GAATTTGCCAGAATTAGAAGTAGTACATTAAGGAAAGAAAAACCTAAATTTTTCTATCCTATTTATGTTAGTCCTGATTT
AAAAGTTATTACTAGTAGTAATATTGATGGATATGTTCCAGTTTATCCAGTAGATAGAAATGGGACAGAATATTCTTGGA
AAGTAATTCCTGAAACATTTGAGGAAAATAATAAACTTGGGTTATATGTAGCTCAAAATACAGAAGATGGTATCAGAATT
TTTAATAAATTTTATGAGCAACAAGTTTTTCAAAATGTATGGATAGATAAAAAATATTATCCCGAATTTAATGGCACTAA
TTTATTAAAAGGTATTTTAGGTAATAGTATATTTTCTTATCCAAAATCTATATATGCAGTACAAGATTATATAAAAATCA
CATCAAAAAAAGATGATTATATTCTAGACTACTTCGCAGGTTCAGGCACAACCGCCCACGCCGTGATTAACCTAAACCGT
GAAGATGGCGGTAATCGCAAATATATCTTGGTGGAGCAGGGCGAGTATTTTGATACGGTGCTAAAACCACGCGTGCAAAA
AGTGATTTTCGCCAAAGAATGGAAAGACGGCAAACCGCAAGCGGACAATGGGGCATTTGGTGGCGTATCGCAAATTATCA
AAGTGCTAAAACTGGAAAGCTACGAAGACACACTCAATAATCTAGAGTTACGCAAACCTGCACAAGATTTGGCGGATATA
GGCTTATCAGAGACGGTGCAAAACGACTATTTATTGCACTATATGCTAGACGTGGAAAGCCGTGAGAGTTTGCTCAATAC
GCAACATTTCACCAAACCGTTTGATTATCAGCTCAATATCGCTACCACGTCTGCGGGAGCGTATGAGACGAAAACCATTG
ATTTGGTGGAAACCTTTAACTATTTGATTGGCTTGCGAGTCAGTGAAATCAACGGCAAGCGTGAAAATGGCTTGGTAACA
GTGCAAGGCACGAATGCAAGCGGTGAAAAAACGCTCGTGATTTGGCGTGATTGTGAAAAATACGACTATGACAGACTCAA
TGATTATCTAAATCGCCATAAAATCAATCCGCAAGAAAGCGAATTTGACGTGGTTTATATCAACGGTGATCACAATGTGC
CGACCGTTTTTGCAGGCAATGATGAAAGCGTAAAAATGCTGAAAGTGCGGTCGATTGAAGCAGAGTTTTTACAACGAATG
TTTGAATGA

Upstream 100 bases:

>100_bases
TCCTAATGACAGCACAGCACAGCACAGCACAGCACAGCACAGCACAGCACAGCACAGCACAGCACAGCACAGCACAGCAC
ATGCACAACTTATCACTAAA

Downstream 100 bases:

>100_bases
CAAATCTCCCCTAACCCCTCTTTACTAAAGAGGGGAATAGATTTGTGAAAAATTGTAGATCTCTATTCTTCATCAAGATC
CCCCCTTCTATTTACTAAAG

Product: putative methylation subunit, type III restriction-modification system

Products: NA

Alternate protein names: M.KpnI; Adenine-specific methyltransferase KpnI [H]

Number of amino acids: Translated: 962; Mature: 962

Protein sequence:

>962_residues
MQEIFQINQPDLDFGIYRILNSRQNEIQQFLTRTLPNNINGAFNGNFEQANSVYNHLYTFFSRYYDQGDFISQRRYKGDT
YAIPYAGEEVMLHWANKDQYYTKSGENFSNYAFTLENGKKVQFRLVEADTAKDNRKDNDQTRVFALIEPRIQTATDENGD
EIQTDILPFDIQGDLLTIRFEYKAVNKKEKQADYITQAVEKIKTFAISDEFLDILKPMPTEKNKTHTLLEKYLTDYTAKN
TADYFIHKNLGKFLNQELDFYIKNEVMNLDNIQDSTDFSHIEQNLQTIKTLKTVAKEIIAFLAQLEDFQKKLWLKKKFVA
GCHYLITLDHLTEEQVQQALDNPKQTAQWKALFNVETSGQNVAEICKNYPNLVVDTSLFEPKFQAEVLNELHDLDEKTNG
LLIHSDNFQALNLLQEKYKEQVKCIYIDPPYNTNASEIIYKNGYKHSSWNSLIENRVSLSRKLLSNEGFFAGTIDYAELF
SFGKILDEEFGELSRVGIVSILINPKGRQHERFFSASSEYMIVYTPSPNSARFNQTTIDAEKRKTFNLFDEDKKERYRLD
EFARIRSSTLRKEKPKFFYPIYVSPDLKVITSSNIDGYVPVYPVDRNGTEYSWKVIPETFEENNKLGLYVAQNTEDGIRI
FNKFYEQQVFQNVWIDKKYYPEFNGTNLLKGILGNSIFSYPKSIYAVQDYIKITSKKDDYILDYFAGSGTTAHAVINLNR
EDGGNRKYILVEQGEYFDTVLKPRVQKVIFAKEWKDGKPQADNGAFGGVSQIIKVLKLESYEDTLNNLELRKPAQDLADI
GLSETVQNDYLLHYMLDVESRESLLNTQHFTKPFDYQLNIATTSAGAYETKTIDLVETFNYLIGLRVSEINGKRENGLVT
VQGTNASGEKTLVIWRDCEKYDYDRLNDYLNRHKINPQESEFDVVYINGDHNVPTVFAGNDESVKMLKVRSIEAEFLQRM
FE

Sequences:

>Translated_962_residues
MQEIFQINQPDLDFGIYRILNSRQNEIQQFLTRTLPNNINGAFNGNFEQANSVYNHLYTFFSRYYDQGDFISQRRYKGDT
YAIPYAGEEVMLHWANKDQYYTKSGENFSNYAFTLENGKKVQFRLVEADTAKDNRKDNDQTRVFALIEPRIQTATDENGD
EIQTDILPFDIQGDLLTIRFEYKAVNKKEKQADYITQAVEKIKTFAISDEFLDILKPMPTEKNKTHTLLEKYLTDYTAKN
TADYFIHKNLGKFLNQELDFYIKNEVMNLDNIQDSTDFSHIEQNLQTIKTLKTVAKEIIAFLAQLEDFQKKLWLKKKFVA
GCHYLITLDHLTEEQVQQALDNPKQTAQWKALFNVETSGQNVAEICKNYPNLVVDTSLFEPKFQAEVLNELHDLDEKTNG
LLIHSDNFQALNLLQEKYKEQVKCIYIDPPYNTNASEIIYKNGYKHSSWNSLIENRVSLSRKLLSNEGFFAGTIDYAELF
SFGKILDEEFGELSRVGIVSILINPKGRQHERFFSASSEYMIVYTPSPNSARFNQTTIDAEKRKTFNLFDEDKKERYRLD
EFARIRSSTLRKEKPKFFYPIYVSPDLKVITSSNIDGYVPVYPVDRNGTEYSWKVIPETFEENNKLGLYVAQNTEDGIRI
FNKFYEQQVFQNVWIDKKYYPEFNGTNLLKGILGNSIFSYPKSIYAVQDYIKITSKKDDYILDYFAGSGTTAHAVINLNR
EDGGNRKYILVEQGEYFDTVLKPRVQKVIFAKEWKDGKPQADNGAFGGVSQIIKVLKLESYEDTLNNLELRKPAQDLADI
GLSETVQNDYLLHYMLDVESRESLLNTQHFTKPFDYQLNIATTSAGAYETKTIDLVETFNYLIGLRVSEINGKRENGLVT
VQGTNASGEKTLVIWRDCEKYDYDRLNDYLNRHKINPQESEFDVVYINGDHNVPTVFAGNDESVKMLKVRSIEAEFLQRM
FE
>Mature_962_residues
MQEIFQINQPDLDFGIYRILNSRQNEIQQFLTRTLPNNINGAFNGNFEQANSVYNHLYTFFSRYYDQGDFISQRRYKGDT
YAIPYAGEEVMLHWANKDQYYTKSGENFSNYAFTLENGKKVQFRLVEADTAKDNRKDNDQTRVFALIEPRIQTATDENGD
EIQTDILPFDIQGDLLTIRFEYKAVNKKEKQADYITQAVEKIKTFAISDEFLDILKPMPTEKNKTHTLLEKYLTDYTAKN
TADYFIHKNLGKFLNQELDFYIKNEVMNLDNIQDSTDFSHIEQNLQTIKTLKTVAKEIIAFLAQLEDFQKKLWLKKKFVA
GCHYLITLDHLTEEQVQQALDNPKQTAQWKALFNVETSGQNVAEICKNYPNLVVDTSLFEPKFQAEVLNELHDLDEKTNG
LLIHSDNFQALNLLQEKYKEQVKCIYIDPPYNTNASEIIYKNGYKHSSWNSLIENRVSLSRKLLSNEGFFAGTIDYAELF
SFGKILDEEFGELSRVGIVSILINPKGRQHERFFSASSEYMIVYTPSPNSARFNQTTIDAEKRKTFNLFDEDKKERYRLD
EFARIRSSTLRKEKPKFFYPIYVSPDLKVITSSNIDGYVPVYPVDRNGTEYSWKVIPETFEENNKLGLYVAQNTEDGIRI
FNKFYEQQVFQNVWIDKKYYPEFNGTNLLKGILGNSIFSYPKSIYAVQDYIKITSKKDDYILDYFAGSGTTAHAVINLNR
EDGGNRKYILVEQGEYFDTVLKPRVQKVIFAKEWKDGKPQADNGAFGGVSQIIKVLKLESYEDTLNNLELRKPAQDLADI
GLSETVQNDYLLHYMLDVESRESLLNTQHFTKPFDYQLNIATTSAGAYETKTIDLVETFNYLIGLRVSEINGKRENGLVT
VQGTNASGEKTLVIWRDCEKYDYDRLNDYLNRHKINPQESEFDVVYINGDHNVPTVFAGNDESVKMLKVRSIEAEFLQRM
FE

Specific function: This methylase recognizes the double-stranded sequence GGTACC, causes specific methylation on A-4 on both strands, and protects the DNA from cleavage by the KpnI endonuclease [H]

COG id: COG2189

COG function: function code L; Adenine specific DNA methylase Mod

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002295
- InterPro:   IPR002941
- InterPro:   IPR002052 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 111682; Mature: 111682

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEIFQINQPDLDFGIYRILNSRQNEIQQFLTRTLPNNINGAFNGNFEQANSVYNHLYTF
CCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
FSRYYDQGDFISQRRYKGDTYAIPYAGEEVMLHWANKDQYYTKSGENFSNYAFTLENGKK
HHHHCCCCCHHHHHCCCCCEEEECCCCCEEEEEECCCCCEEECCCCCCCCEEEEECCCCE
VQFRLVEADTAKDNRKDNDQTRVFALIEPRIQTATDENGDEIQTDILPFDIQGDLLTIRF
EEEEEEECCCCCCCCCCCCCEEEEEEECCCHHCCCCCCCCEEECCEEEEEECCCEEEEEE
EYKAVNKKEKQADYITQAVEKIKTFAISDEFLDILKPMPTEKNKTHTLLEKYLTDYTAKN
EEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCC
TADYFIHKNLGKFLNQELDFYIKNEVMNLDNIQDSTDFSHIEQNLQTIKTLKTVAKEIIA
CHHHHHHHHHHHHHHHHHCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FLAQLEDFQKKLWLKKKFVAGCHYLITLDHLTEEQVQQALDNPKQTAQWKALFNVETSGQ
HHHHHHHHHHHHHHHHHHHHCCEEEEEEHHCCHHHHHHHHCCCHHCCEEEEEEEEECCCC
NVAEICKNYPNLVVDTSLFEPKFQAEVLNELHDLDEKTNGLLIHSDNFQALNLLQEKYKE
HHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCHHCCCEEEECCCHHHHHHHHHHHHC
QVKCIYIDPPYNTNASEIIYKNGYKHSSWNSLIENRVSLSRKLLSNEGFFAGTIDYAELF
CEEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHH
SFGKILDEEFGELSRVGIVSILINPKGRQHERFFSASSEYMIVYTPSPNSARFNQTTIDA
HHHHHHHHHHHHHHHCEEEEEEECCCCCCHHHHHCCCCCEEEEEECCCCCCCCCCEECCH
EKRKTFNLFDEDKKERYRLDEFARIRSSTLRKEKPKFFYPIYVSPDLKVITSSNIDGYVP
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEECCCCCEEE
VYPVDRNGTEYSWKVIPETFEENNKLGLYVAQNTEDGIRIFNKFYEQQVFQNVWIDKKYY
EEEECCCCCEEEEEECCHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCC
PEFNGTNLLKGILGNSIFSYPKSIYAVQDYIKITSKKDDYILDYFAGSGTTAHAVINLNR
CCCCCCHHHHHHHCCHHHHCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEEEEC
EDGGNRKYILVEQGEYFDTVLKPRVQKVIFAKEWKDGKPQADNGAFGGVSQIIKVLKLES
CCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCC
YEDTLNNLELRKPAQDLADIGLSETVQNDYLLHYMLDVESRESLLNTQHFTKPFDYQLNI
HHHHHHCCCCCCCHHHHHHCCCHHHHCCCEEEEEEECCHHHHHHHHHHHCCCCCCEEEEE
ATTSAGAYETKTIDLVETFNYLIGLRVSEINGKRENGLVTVQGTNASGEKTLVIWRDCEK
EEECCCCCCCHHHHHHHHHHHHHEEEEEECCCCCCCCEEEEEECCCCCCEEEEEEECCCC
YDYDRLNDYLNRHKINPQESEFDVVYINGDHNVPTVFAGNDESVKMLKVRSIEAEFLQRM
CCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCEEEECCCCCEEEEEEHHHHHHHHHHH
FE
CC
>Mature Secondary Structure
MQEIFQINQPDLDFGIYRILNSRQNEIQQFLTRTLPNNINGAFNGNFEQANSVYNHLYTF
CCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
FSRYYDQGDFISQRRYKGDTYAIPYAGEEVMLHWANKDQYYTKSGENFSNYAFTLENGKK
HHHHCCCCCHHHHHCCCCCEEEECCCCCEEEEEECCCCCEEECCCCCCCCEEEEECCCCE
VQFRLVEADTAKDNRKDNDQTRVFALIEPRIQTATDENGDEIQTDILPFDIQGDLLTIRF
EEEEEEECCCCCCCCCCCCCEEEEEEECCCHHCCCCCCCCEEECCEEEEEECCCEEEEEE
EYKAVNKKEKQADYITQAVEKIKTFAISDEFLDILKPMPTEKNKTHTLLEKYLTDYTAKN
EEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCC
TADYFIHKNLGKFLNQELDFYIKNEVMNLDNIQDSTDFSHIEQNLQTIKTLKTVAKEIIA
CHHHHHHHHHHHHHHHHHCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FLAQLEDFQKKLWLKKKFVAGCHYLITLDHLTEEQVQQALDNPKQTAQWKALFNVETSGQ
HHHHHHHHHHHHHHHHHHHHCCEEEEEEHHCCHHHHHHHHCCCHHCCEEEEEEEEECCCC
NVAEICKNYPNLVVDTSLFEPKFQAEVLNELHDLDEKTNGLLIHSDNFQALNLLQEKYKE
HHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCHHCCCEEEECCCHHHHHHHHHHHHC
QVKCIYIDPPYNTNASEIIYKNGYKHSSWNSLIENRVSLSRKLLSNEGFFAGTIDYAELF
CEEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHH
SFGKILDEEFGELSRVGIVSILINPKGRQHERFFSASSEYMIVYTPSPNSARFNQTTIDA
HHHHHHHHHHHHHHHCEEEEEEECCCCCCHHHHHCCCCCEEEEEECCCCCCCCCCEECCH
EKRKTFNLFDEDKKERYRLDEFARIRSSTLRKEKPKFFYPIYVSPDLKVITSSNIDGYVP
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEECCCCCEEE
VYPVDRNGTEYSWKVIPETFEENNKLGLYVAQNTEDGIRIFNKFYEQQVFQNVWIDKKYY
EEEECCCCCEEEEEECCHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCC
PEFNGTNLLKGILGNSIFSYPKSIYAVQDYIKITSKKDDYILDYFAGSGTTAHAVINLNR
CCCCCCHHHHHHHCCHHHHCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEEEEC
EDGGNRKYILVEQGEYFDTVLKPRVQKVIFAKEWKDGKPQADNGAFGGVSQIIKVLKLES
CCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCC
YEDTLNNLELRKPAQDLADIGLSETVQNDYLLHYMLDVESRESLLNTQHFTKPFDYQLNI
HHHHHHCCCCCCCHHHHHHCCCHHHHCCCEEEEEEECCHHHHHHHHHHHCCCCCCEEEEE
ATTSAGAYETKTIDLVETFNYLIGLRVSEINGKRENGLVTVQGTNASGEKTLVIWRDCEK
EEECCCCCCCHHHHHHHHHHHHHEEEEEECCCCCCCCEEEEEECCCCCCEEEEEEECCCC
YDYDRLNDYLNRHKINPQESEFDVVYINGDHNVPTVFAGNDESVKMLKVRSIEAEFLQRM
CCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCEEEECCCCCEEEEEEHHHHHHHHHHH
FE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1754388 [H]