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The map label for this gene is mdeA [H]

Identifier: 126208181

GI number: 126208181

Start: 803799

End: 805067

Strand: Reverse

Name: mdeA [H]

Synonym: APL_0701

Alternate gene names: 126208181

Gene position: 805067-803799 (Counterclockwise)

Preceding gene: 126208182

Following gene: 126208180

Centisome position: 35.4

GC content: 44.29

Gene sequence:

>1269_bases
ATGAATTTTGAAACACTTTGCCTACATGCAGGCTATACGCCGAAAAACGGCGAGCCGAGAGCTTTACCTATCGTACAAAG
TACGACATTTACCTATGATAGTGCGGAATCTATCGGGAAATTATTCGATTTAGAAGAAGCGGGCTTCTTTTATACTCGTT
TAGCAAATCCGACGACGAATGCGGTGGAAGAAAAAATTGCGGCGTTAGAAGGCGGGGTTGCGGCTATGTGTACCGCATCG
GGACAGTCTGCGGTTTTCTATGCGCTGATGAATTTACTTGAATCCGGCGACCATTTTATTTCAGCATCCAGCATTTACGG
CGGCAGTTATAACCTATTCGCACATACGTTCCGCAAAATGGGGATTGAAGTGACTTTTGTCGATCAATTTGCCCCGATTG
AAGAATTGAAAAAAGCGATTCGTCCGAATACTAAAGCGGTCTTCGGTGAAACTATTGCAAATCCGGCGTTACGCGTATTG
GATATTGAAAAATTTGCCGCATTAGCGCAAGCGGCGGAATGTCCGTTAGTTGTCGATAATACTTTTGCTACGCCGTATTT
CTGCCGTCCTTTTGAATTCGGCGCCAATATAGTGGTACACAGTTCATCAAAATATCTTGACGGTCATGCGGTCGCATTGG
GCGGGGTGATTATTGACGGCGGAAATTTCGATTGGAATAACCCTAAATTTAAAGCCTTCCACCAACCGGATGAAACCTAT
CACGGACTAGTTTATACCGAAACGTTCGGTAAAGCGGCGTATATGGTAAAAGCTCGAGTGCAATTGATGAGAGATTTAGG
CGCGACGCCCGCACCGCAAAACAGTTTCTTACTGAACTTAGGGTTGGAAACATTATCCCTCCGTATGAAACAACATTTTG
CCAATGCTAAGGCGGTTGCGGAATTCTTAGAAGCGCATCCTCAAGTAAAAAGCGTATATTATCCGGCATTGGCAAGTTCA
CCGGATTTCGCATTACAGCAAAAATATACGCCTAACGGCTTATGCGGCGTGCTTTCTTTTGAATTAAAAGGTAATAAAGA
AACTGCGATGAAATGGCTGAATGCGCTTAAGTTAGTTTCTCGAGAAGTGCATGTGGCGGATATTCGTACTTGTGCGTTAC
ATCCGGCAACTTCTACGCATCGTCAGTTAAGTACGGAAGATCTCAAAGCGGCGAATATTTCCGAAACATTGATTCGTCTT
TCGGTAGGGATCGAAACGCTGGAAGATATTCTAGCGGATTTAACTCAAGCATTTGAGCAAGCTGTGTAA

Upstream 100 bases:

>100_bases
AAAGGAAAATCCTAGCGGATCATTCGCTAAAACTAAGCGTATAACTGTATCGTTTTCTGTTACAATCAAAAAACTTTACT
CAACCAAAAAGGGAATATTT

Downstream 100 bases:

>100_bases
AGACTTAATATGAATGTATAAGCGGTCGTAAATTTTCCTAGACTTACGACCGCTTGTTTATGAAGGGAAGAGAGAAAATC
AATCCATTACGTTTGTATTA

Product: O-acetylhomoserine (thiol)-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MNFETLCLHAGYTPKNGEPRALPIVQSTTFTYDSAESIGKLFDLEEAGFFYTRLANPTTNAVEEKIAALEGGVAAMCTAS
GQSAVFYALMNLLESGDHFISASSIYGGSYNLFAHTFRKMGIEVTFVDQFAPIEELKKAIRPNTKAVFGETIANPALRVL
DIEKFAALAQAAECPLVVDNTFATPYFCRPFEFGANIVVHSSSKYLDGHAVALGGVIIDGGNFDWNNPKFKAFHQPDETY
HGLVYTETFGKAAYMVKARVQLMRDLGATPAPQNSFLLNLGLETLSLRMKQHFANAKAVAEFLEAHPQVKSVYYPALASS
PDFALQQKYTPNGLCGVLSFELKGNKETAMKWLNALKLVSREVHVADIRTCALHPATSTHRQLSTEDLKAANISETLIRL
SVGIETLEDILADLTQAFEQAV

Sequences:

>Translated_422_residues
MNFETLCLHAGYTPKNGEPRALPIVQSTTFTYDSAESIGKLFDLEEAGFFYTRLANPTTNAVEEKIAALEGGVAAMCTAS
GQSAVFYALMNLLESGDHFISASSIYGGSYNLFAHTFRKMGIEVTFVDQFAPIEELKKAIRPNTKAVFGETIANPALRVL
DIEKFAALAQAAECPLVVDNTFATPYFCRPFEFGANIVVHSSSKYLDGHAVALGGVIIDGGNFDWNNPKFKAFHQPDETY
HGLVYTETFGKAAYMVKARVQLMRDLGATPAPQNSFLLNLGLETLSLRMKQHFANAKAVAEFLEAHPQVKSVYYPALASS
PDFALQQKYTPNGLCGVLSFELKGNKETAMKWLNALKLVSREVHVADIRTCALHPATSTHRQLSTEDLKAANISETLIRL
SVGIETLEDILADLTQAFEQAV
>Mature_422_residues
MNFETLCLHAGYTPKNGEPRALPIVQSTTFTYDSAESIGKLFDLEEAGFFYTRLANPTTNAVEEKIAALEGGVAAMCTAS
GQSAVFYALMNLLESGDHFISASSIYGGSYNLFAHTFRKMGIEVTFVDQFAPIEELKKAIRPNTKAVFGETIANPALRVL
DIEKFAALAQAAECPLVVDNTFATPYFCRPFEFGANIVVHSSSKYLDGHAVALGGVIIDGGNFDWNNPKFKAFHQPDETY
HGLVYTETFGKAAYMVKARVQLMRDLGATPAPQNSFLLNLGLETLSLRMKQHFANAKAVAEFLEAHPQVKSVYYPALASS
PDFALQQKYTPNGLCGVLSFELKGNKETAMKWLNALKLVSREVHVADIRTCALHPATSTHRQLSTEDLKAANISETLIRL
SVGIETLEDILADLTQAFEQAV

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=423, Percent_Identity=35.4609929078014, Blast_Score=222, Evalue=4e-58,
Organism=Homo sapiens, GI299473759, Length=423, Percent_Identity=33.3333333333333, Blast_Score=189, Evalue=6e-48,
Organism=Homo sapiens, GI25453487, Length=422, Percent_Identity=30.8056872037915, Blast_Score=169, Evalue=5e-42,
Organism=Escherichia coli, GI1790375, Length=374, Percent_Identity=35.8288770053476, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1789383, Length=426, Percent_Identity=24.6478873239437, Blast_Score=130, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17538055, Length=427, Percent_Identity=36.0655737704918, Blast_Score=229, Evalue=3e-60,
Organism=Caenorhabditis elegans, GI17560056, Length=426, Percent_Identity=36.3849765258216, Blast_Score=228, Evalue=4e-60,
Organism=Caenorhabditis elegans, GI71980708, Length=413, Percent_Identity=32.9297820823245, Blast_Score=219, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6323333, Length=434, Percent_Identity=44.0092165898618, Blast_Score=318, Evalue=8e-88,
Organism=Saccharomyces cerevisiae, GI6319307, Length=427, Percent_Identity=33.0210772833724, Blast_Score=206, Evalue=4e-54,
Organism=Saccharomyces cerevisiae, GI6321254, Length=443, Percent_Identity=25.7336343115124, Blast_Score=116, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6321170, Length=283, Percent_Identity=27.208480565371, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321904, Length=435, Percent_Identity=27.5862068965517, Blast_Score=98, Evalue=3e-21,
Organism=Drosophila melanogaster, GI19922554, Length=426, Percent_Identity=34.9765258215962, Blast_Score=231, Evalue=7e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46186; Mature: 46186

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFETLCLHAGYTPKNGEPRALPIVQSTTFTYDSAESIGKLFDLEEAGFFYTRLANPTTN
CCCCEEEEECCCCCCCCCCCEEEEEEECEECCCCHHHHHHHHCHHCCCEEEEEECCCCHH
AVEEKIAALEGGVAAMCTASGQSAVFYALMNLLESGDHFISASSIYGGSYNLFAHTFRKM
HHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEECEECCCCHHHHHHHHHHC
GIEVTFVDQFAPIEELKKAIRPNTKAVFGETIANPALRVLDIEKFAALAQAAECPLVVDN
CCEEEEECCCCCHHHHHHHHCCCCCEEHHHHHCCCCEEEEEHHHHHHHHHHCCCCEEEEC
TFATPYFCRPFEFGANIVVHSSSKYLDGHAVALGGVIIDGGNFDWNNPKFKAFHQPDETY
CCCCCCEECCHHCCCEEEEECCCCCCCCCEEEECCEEEECCCCCCCCCCEEEECCCCCCC
HGLVYTETFGKAAYMVKARVQLMRDLGATPAPQNSFLLNLGLETLSLRMKQHFANAKAVA
CCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHHHHHHCCHHHHH
EFLEAHPQVKSVYYPALASSPDFALQQKYTPNGLCGVLSFELKGNKETAMKWLNALKLVS
HHHHCCCCHHHEECCCCCCCCCCHHHCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHH
REVHVADIRTCALHPATSTHRQLSTEDLKAANISETLIRLSVGIETLEDILADLTQAFEQ
CCCCHHHHHHEEECCCCCHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AV
CC
>Mature Secondary Structure
MNFETLCLHAGYTPKNGEPRALPIVQSTTFTYDSAESIGKLFDLEEAGFFYTRLANPTTN
CCCCEEEEECCCCCCCCCCCEEEEEEECEECCCCHHHHHHHHCHHCCCEEEEEECCCCHH
AVEEKIAALEGGVAAMCTASGQSAVFYALMNLLESGDHFISASSIYGGSYNLFAHTFRKM
HHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEECEECCCCHHHHHHHHHHC
GIEVTFVDQFAPIEELKKAIRPNTKAVFGETIANPALRVLDIEKFAALAQAAECPLVVDN
CCEEEEECCCCCHHHHHHHHCCCCCEEHHHHHCCCCEEEEEHHHHHHHHHHCCCCEEEEC
TFATPYFCRPFEFGANIVVHSSSKYLDGHAVALGGVIIDGGNFDWNNPKFKAFHQPDETY
CCCCCCEECCHHCCCEEEEECCCCCCCCCEEEECCEEEECCCCCCCCCCEEEECCCCCCC
HGLVYTETFGKAAYMVKARVQLMRDLGATPAPQNSFLLNLGLETLSLRMKQHFANAKAVA
CCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHHHHHHCCHHHHH
EFLEAHPQVKSVYYPALASSPDFALQQKYTPNGLCGVLSFELKGNKETAMKWLNALKLVS
HHHHCCCCHHHEECCCCCCCCCCHHHCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHH
REVHVADIRTCALHPATSTHRQLSTEDLKAANISETLIRLSVGIETLEDILADLTQAFEQ
CCCCHHHHHHEEECCCCCHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]