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The map label for this gene is manB [H]

Identifier: 126208132

GI number: 126208132

Start: 745538

End: 746896

Strand: Reverse

Name: manB [H]

Synonym: APL_0652

Alternate gene names: 126208132

Gene position: 746896-745538 (Counterclockwise)

Preceding gene: 126208133

Following gene: 126208131

Centisome position: 32.84

GC content: 42.02

Gene sequence:

>1359_bases
ATGAGTAAACTCACTTGTTTTAAAGCCTATGATATTCGCGGTCAATTAGGCACAGAATTGAATACGGATATTGCATACCG
TATCGGACGTGCTTTTGGACAATATCTCAAACCGAAAAGTATTGTTGTCGGTGGGGACGTTCGTTTAACAAGTAACGAAT
TAAAAAGTGCGGTGACTAACGGCTTGTTAGATTCGGGAGTGAACGTCATTGATTTAGGCCTTACCGGTACGGAAGAAGTT
TATTTCGCCACATCGTTCCTTAAAACCGACGGCGGTATTCAAGTAACGGCAAGCCATAACCCGATGGATTATAACGGGAT
GAAGTTAGTCAGAGAAGGTTCTCGTCCGATTAGTGCCGATACGGGCTTAGCCGATATCCAACGCCTAGCCGAAGAGAATA
ATTTTCCACCGGTTACACAAAGAGGAACCTATACGCAAAAAAGCGTGCAGGGCGAATATGTAGAACACTTACTCTCTTAT
ATCGACTTAGATAAACTTACCCCGAAAAAACTAGTAATTAACTCGGGTAATGGTGCGGCAGGTCATATCATTGATGCGAT
TGAAGCACAATTTAAAGCGCGTAACGTGCCGGTAGAATTTGTTAAAGTACATAATAATCCGGACGGCAATTTCCCTAACG
GTATTCCTAACCCGATTCTGCATGAAAACCGTGAGGATTCAATTAAAGCCGTATTAGCGAATCATGCGGATGCGGGGATT
GCATTCGACGGCGATTTCGACCGTTGCTTCTTCTTTGATGAAAAAGGCGGGTTTATCGAAGGTTACTATGTAGTAGGCTT
ATTATCTCAAGCATTCTTAGCGAAAAATAAAGGTGCTAAGATTATTTATGATCCGCGTTTAATTTGGAATACGGAAGAAT
TAGTATCGGAAGCCGGCGGCGAAGCGGTGATGTCAAAATCGGGTCACTCGTTTATTAAAGAGAAAATGCGCGCAGTAGAT
GCGATTTATGGTGGTGAAATGTCTGCTCACCATTATTTCCGTGATTTCTTCTACTGTGATAGCGGTATGATTCCATGGCT
ATTAACGTTAGAACTACTATGTACGACAGGTAAACCGTTAAGCGAATTAGTCGGTACTCGTTTGGATAAATTCCCGTCAC
CGGGTGAAATTAACAGTAAATTAACCGATGCTAAAGCGGCTATCGAACGTGTTAAAGAAGCGTATAAAGACGGTAAAGTC
GAAACCTTAGACGGTATCAGCGTTGAATTTGAAAACTGGCGTTTCAACTTACGCAGTTCAAATACCGAACCTGTTGTACG
TTTAAATTTAGAAACTCGCGGCGATAAAGTGTTAATGCAAGAGAAAACTGATGAAACCCTTGCATTACTTCGCCAGTAA

Upstream 100 bases:

>100_bases
CGAGAAGAAATTTATCAAAGAATCAAAGCATTAGCGGATGATGTCGCTAGTGACACGCAACAGTAAATATTTTTATCACT
AACAGAGTAAGGACTAATCT

Downstream 100 bases:

>100_bases
CGAGTAAAATTTAGATGAAATTAGACCGCTTGCTTATACGAGCGGTCTCTATTATGAGATACGATATCGTATTTGATTTA
AACGGTGCTATGCACCTTAT

Product: phosphomannomutase

Products: NA

Alternate protein names: PMM [H]

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MSKLTCFKAYDIRGQLGTELNTDIAYRIGRAFGQYLKPKSIVVGGDVRLTSNELKSAVTNGLLDSGVNVIDLGLTGTEEV
YFATSFLKTDGGIQVTASHNPMDYNGMKLVREGSRPISADTGLADIQRLAEENNFPPVTQRGTYTQKSVQGEYVEHLLSY
IDLDKLTPKKLVINSGNGAAGHIIDAIEAQFKARNVPVEFVKVHNNPDGNFPNGIPNPILHENREDSIKAVLANHADAGI
AFDGDFDRCFFFDEKGGFIEGYYVVGLLSQAFLAKNKGAKIIYDPRLIWNTEELVSEAGGEAVMSKSGHSFIKEKMRAVD
AIYGGEMSAHHYFRDFFYCDSGMIPWLLTLELLCTTGKPLSELVGTRLDKFPSPGEINSKLTDAKAAIERVKEAYKDGKV
ETLDGISVEFENWRFNLRSSNTEPVVRLNLETRGDKVLMQEKTDETLALLRQ

Sequences:

>Translated_452_residues
MSKLTCFKAYDIRGQLGTELNTDIAYRIGRAFGQYLKPKSIVVGGDVRLTSNELKSAVTNGLLDSGVNVIDLGLTGTEEV
YFATSFLKTDGGIQVTASHNPMDYNGMKLVREGSRPISADTGLADIQRLAEENNFPPVTQRGTYTQKSVQGEYVEHLLSY
IDLDKLTPKKLVINSGNGAAGHIIDAIEAQFKARNVPVEFVKVHNNPDGNFPNGIPNPILHENREDSIKAVLANHADAGI
AFDGDFDRCFFFDEKGGFIEGYYVVGLLSQAFLAKNKGAKIIYDPRLIWNTEELVSEAGGEAVMSKSGHSFIKEKMRAVD
AIYGGEMSAHHYFRDFFYCDSGMIPWLLTLELLCTTGKPLSELVGTRLDKFPSPGEINSKLTDAKAAIERVKEAYKDGKV
ETLDGISVEFENWRFNLRSSNTEPVVRLNLETRGDKVLMQEKTDETLALLRQ
>Mature_451_residues
SKLTCFKAYDIRGQLGTELNTDIAYRIGRAFGQYLKPKSIVVGGDVRLTSNELKSAVTNGLLDSGVNVIDLGLTGTEEVY
FATSFLKTDGGIQVTASHNPMDYNGMKLVREGSRPISADTGLADIQRLAEENNFPPVTQRGTYTQKSVQGEYVEHLLSYI
DLDKLTPKKLVINSGNGAAGHIIDAIEAQFKARNVPVEFVKVHNNPDGNFPNGIPNPILHENREDSIKAVLANHADAGIA
FDGDFDRCFFFDEKGGFIEGYYVVGLLSQAFLAKNKGAKIIYDPRLIWNTEELVSEAGGEAVMSKSGHSFIKEKMRAVDA
IYGGEMSAHHYFRDFFYCDSGMIPWLLTLELLCTTGKPLSELVGTRLDKFPSPGEINSKLTDAKAAIERVKEAYKDGKVE
TLDGISVEFENWRFNLRSSNTEPVVRLNLETRGDKVLMQEKTDETLALLRQ

Specific function: Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS O antigen [H]

COG id: COG1109

COG function: function code G; Phosphomannomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphohexose mutase family [H]

Homologues:

Organism=Escherichia coli, GI1788361, Length=456, Percent_Identity=67.1052631578947, Blast_Score=640, Evalue=0.0,
Organism=Escherichia coli, GI1789566, Length=449, Percent_Identity=24.9443207126949, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005844
- InterPro:   IPR016055
- InterPro:   IPR005845
- InterPro:   IPR005846
- InterPro:   IPR005843
- InterPro:   IPR016066
- InterPro:   IPR005841 [H]

Pfam domain/function: PF02878 PGM_PMM_I; PF02879 PGM_PMM_II; PF02880 PGM_PMM_III; PF00408 PGM_PMM_IV [H]

EC number: =5.4.2.8 [H]

Molecular weight: Translated: 49997; Mature: 49866

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00141 ASP_PROTEASE ; PS00710 PGM_PMM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLTCFKAYDIRGQLGTELNTDIAYRIGRAFGQYLKPKSIVVGGDVRLTSNELKSAVTN
CCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCEEECHHHHHHHHHH
GLLDSGVNVIDLGLTGTEEVYFATSFLKTDGGIQVTASHNPMDYNGMKLVREGSRPISAD
HHHHCCCCEEEECCCCCCCEEEEEEEEECCCCEEEEECCCCCCCCCHHHHCCCCCCCCCC
TGLADIQRLAEENNFPPVTQRGTYTQKSVQGEYVEHLLSYIDLDKLTPKKLVINSGNGAA
CCHHHHHHHHHCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
GHIIDAIEAQFKARNVPVEFVKVHNNPDGNFPNGIPNPILHENREDSIKAVLANHADAGI
HHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCE
AFDGDFDRCFFFDEKGGFIEGYYVVGLLSQAFLAKNKGAKIIYDPRLIWNTEELVSEAGG
EECCCCCEEEEEECCCCEEEHHHHHHHHHHHHHHCCCCCEEEECCEEEECHHHHHHHCCC
EAVMSKSGHSFIKEKMRAVDAIYGGEMSAHHYFRDFFYCDSGMIPWLLTLELLCTTGKPL
CEEHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHEEECCCCCHHHHHHHHHHCCCCCH
SELVGTRLDKFPSPGEINSKLTDAKAAIERVKEAYKDGKVETLDGISVEFENWRFNLRSS
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECEEEEECCC
NTEPVVRLNLETRGDKVLMQEKTDETLALLRQ
CCCCEEEEEEECCCCEEEEECCCHHHHHHHCC
>Mature Secondary Structure 
SKLTCFKAYDIRGQLGTELNTDIAYRIGRAFGQYLKPKSIVVGGDVRLTSNELKSAVTN
CCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCEEECHHHHHHHHHH
GLLDSGVNVIDLGLTGTEEVYFATSFLKTDGGIQVTASHNPMDYNGMKLVREGSRPISAD
HHHHCCCCEEEECCCCCCCEEEEEEEEECCCCEEEEECCCCCCCCCHHHHCCCCCCCCCC
TGLADIQRLAEENNFPPVTQRGTYTQKSVQGEYVEHLLSYIDLDKLTPKKLVINSGNGAA
CCHHHHHHHHHCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
GHIIDAIEAQFKARNVPVEFVKVHNNPDGNFPNGIPNPILHENREDSIKAVLANHADAGI
HHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCE
AFDGDFDRCFFFDEKGGFIEGYYVVGLLSQAFLAKNKGAKIIYDPRLIWNTEELVSEAGG
EECCCCCEEEEEECCCCEEEHHHHHHHHHHHHHHCCCCCEEEECCEEEECHHHHHHHCCC
EAVMSKSGHSFIKEKMRAVDAIYGGEMSAHHYFRDFFYCDSGMIPWLLTLELLCTTGKPL
CEEHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHEEECCCCCHHHHHHHHHHCCCCCH
SELVGTRLDKFPSPGEINSKLTDAKAAIERVKEAYKDGKVETLDGISVEFENWRFNLRSS
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECEEEEECCC
NTEPVVRLNLETRGDKVLMQEKTDETLALLRQ
CCCCEEEEEEECCCCEEEEECCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1383393 [H]