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The map label for this gene is mlc [H]

Identifier: 126208097

GI number: 126208097

Start: 700861

End: 702051

Strand: Reverse

Name: mlc [H]

Synonym: APL_0615

Alternate gene names: 126208097

Gene position: 702051-700861 (Counterclockwise)

Preceding gene: 126208104

Following gene: 126208096

Centisome position: 30.87

GC content: 35.52

Gene sequence:

>1191_bases
ATGATTTCTGATAATAAAACCCAACACTTAGGCACAATTTATCGCCTTGTTGAACAATTCGAATTAATTTCGCGTACGGA
TCTTGCAAAATTATCCGGTTTTGCGCCCGCTTCCATTACGAATCTGACCAAATCGCTTATCGACCATAAATTTATTTTAG
AACGTTCCGTACAAAATAATACGTCTAGAGGACGTCCCGCCGTCGGTCTTTCGGTGTCTAATTTCTTTTGGCAATTGCTT
TGTTTTACCGTTTCTCCCGAGAAAATTGAAATTTCACTTTGCGAATTAAATGGCAAACCGATTCAATCCAAAGAATATCC
TCTGCTTGAAACGGATTATCCGATACTCGCCGAGCATACTCTTCTCTGCTTAAAAGACTTTTTCTGGCATTCTCCCCTGG
AGGAACATCGTATTTTAGCCGTTTCCGTCAGTGTAATCGGACGGATTAATGCAAATAAAACGGAAATTATTCAATTAGGA
CAAACCGAATTACATTGCCCAATTGTTACTACGTTACAAAACCATTTTGAGCGACCGATTCTACTTAACGAGCATTTTCA
ATTATGGCTGTTTACCGAATCCACATTAGGCAGTTTGATTAGTAATGACAACGTAATTTTCCTACAATTAGACGATTCGG
TGAATTTAAGCGTATTATTAAGAGGAACCTTATTGCATCAGCAATCCAAAATGAATGTAGATAAGATGCTTATGCCTAAA
TTCAGTACGTTAAGCGATGAAACTACCGCTATCGATTGTAATGATATTGAAAAATATCAATTAAAAAATCAAATTACATT
CTCGGCAATTATTCGCTTAATTGATAAATATTTGCCGAATAATTTAGAGCATCAAAATGAAAAAATTCAATTTTTATGTG
AACAGGTGGAATCGAATAATCCCGAAGCGTTACGGATATTATCCCATATCAGTGATAACTTAGCCTATGTATTAATGAAT
CTTATCAATATTTTCTCTACCGAGAAAATTATGTTGAATTCACCGTTGTTACAAATAAAACATAAATTGTTTGAACAAAT
ACAAGCTAAATTGGGCAAGAATTTACTCATTGACAACTCTGTAGATTTAGTTTCCAGTCAATATGCGTGGAACGACCCGC
TGATTGCTTGTTCGGCGATAAAACAAGGGATTTATGAAGGTAATTTGATCAAGGACATCATTCAGCCTTAA

Upstream 100 bases:

>100_bases
TTGTGATTCCCCAATATTGAAGCAAAATAATTATCGTATGGAGTGTTTAAATTAAATTTTTTGACCTGATTTTATCTTTG
GTCTATTTAAGGATTATACT

Downstream 100 bases:

>100_bases
AAAATCCGTTAAAATATAGGCAATTTTGTAACTTTTTTGTTAGAGAATAGCATATGCCTTCATTATTTATTACCGGTACG
GATACCAATGTAGGAAAAAC

Product: putative transcriptional repressor of carbohydrate metabolism

Products: NA

Alternate protein names: Making large colonies protein [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MISDNKTQHLGTIYRLVEQFELISRTDLAKLSGFAPASITNLTKSLIDHKFILERSVQNNTSRGRPAVGLSVSNFFWQLL
CFTVSPEKIEISLCELNGKPIQSKEYPLLETDYPILAEHTLLCLKDFFWHSPLEEHRILAVSVSVIGRINANKTEIIQLG
QTELHCPIVTTLQNHFERPILLNEHFQLWLFTESTLGSLISNDNVIFLQLDDSVNLSVLLRGTLLHQQSKMNVDKMLMPK
FSTLSDETTAIDCNDIEKYQLKNQITFSAIIRLIDKYLPNNLEHQNEKIQFLCEQVESNNPEALRILSHISDNLAYVLMN
LINIFSTEKIMLNSPLLQIKHKLFEQIQAKLGKNLLIDNSVDLVSSQYAWNDPLIACSAIKQGIYEGNLIKDIIQP

Sequences:

>Translated_396_residues
MISDNKTQHLGTIYRLVEQFELISRTDLAKLSGFAPASITNLTKSLIDHKFILERSVQNNTSRGRPAVGLSVSNFFWQLL
CFTVSPEKIEISLCELNGKPIQSKEYPLLETDYPILAEHTLLCLKDFFWHSPLEEHRILAVSVSVIGRINANKTEIIQLG
QTELHCPIVTTLQNHFERPILLNEHFQLWLFTESTLGSLISNDNVIFLQLDDSVNLSVLLRGTLLHQQSKMNVDKMLMPK
FSTLSDETTAIDCNDIEKYQLKNQITFSAIIRLIDKYLPNNLEHQNEKIQFLCEQVESNNPEALRILSHISDNLAYVLMN
LINIFSTEKIMLNSPLLQIKHKLFEQIQAKLGKNLLIDNSVDLVSSQYAWNDPLIACSAIKQGIYEGNLIKDIIQP
>Mature_396_residues
MISDNKTQHLGTIYRLVEQFELISRTDLAKLSGFAPASITNLTKSLIDHKFILERSVQNNTSRGRPAVGLSVSNFFWQLL
CFTVSPEKIEISLCELNGKPIQSKEYPLLETDYPILAEHTLLCLKDFFWHSPLEEHRILAVSVSVIGRINANKTEIIQLG
QTELHCPIVTTLQNHFERPILLNEHFQLWLFTESTLGSLISNDNVIFLQLDDSVNLSVLLRGTLLHQQSKMNVDKMLMPK
FSTLSDETTAIDCNDIEKYQLKNQITFSAIIRLIDKYLPNNLEHQNEKIQFLCEQVESNNPEALRILSHISDNLAYVLMN
LINIFSTEKIMLNSPLLQIKHKLFEQIQAKLGKNLLIDNSVDLVSSQYAWNDPLIACSAIKQGIYEGNLIKDIIQP

Specific function: Transcriptional repressor that regulates the expression of proteins that are part of the phosphotransferase system for sugar uptake. Regulates the expression of malT [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Escherichia coli, GI1787878, Length=408, Percent_Identity=23.5294117647059, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1786891, Length=388, Percent_Identity=19.0721649484536, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: NA

Molecular weight: Translated: 45169; Mature: 45169

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISDNKTQHLGTIYRLVEQFELISRTDLAKLSGFAPASITNLTKSLIDHKFILERSVQNN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
TSRGRPAVGLSVSNFFWQLLCFTVSPEKIEISLCELNGKPIQSKEYPLLETDYPILAEHT
CCCCCCEEECCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHH
LLCLKDFFWHSPLEEHRILAVSVSVIGRINANKTEIIQLGQTELHCPIVTTLQNHFERPI
HHHHHHHHHCCCHHHCEEEEEEEEEHEECCCCHHHEEEECCCCCCCHHHHHHHHHCCCCE
LLNEHFQLWLFTESTLGSLISNDNVIFLQLDDSVNLSVLLRGTLLHQQSKMNVDKMLMPK
EECCCEEEEEEECHHHHHHHCCCCEEEEEECCCCCEEEEEEHHHHHHHHHCCHHHHHCCC
FSTLSDETTAIDCNDIEKYQLKNQITFSAIIRLIDKYLPNNLEHQNEKIQFLCEQVESNN
CCCCCCCCCEECCCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC
PEALRILSHISDNLAYVLMNLINIFSTEKIMLNSPLLQIKHKLFEQIQAKLGKNLLIDNS
CHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCCHHHHHHHHHHHHHHHHCCCEEECCC
VDLVSSQYAWNDPLIACSAIKQGIYEGNLIKDIIQP
HHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHCC
>Mature Secondary Structure
MISDNKTQHLGTIYRLVEQFELISRTDLAKLSGFAPASITNLTKSLIDHKFILERSVQNN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
TSRGRPAVGLSVSNFFWQLLCFTVSPEKIEISLCELNGKPIQSKEYPLLETDYPILAEHT
CCCCCCEEECCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHH
LLCLKDFFWHSPLEEHRILAVSVSVIGRINANKTEIIQLGQTELHCPIVTTLQNHFERPI
HHHHHHHHHCCCHHHCEEEEEEEEEHEECCCCHHHEEEECCCCCCCHHHHHHHHHCCCCE
LLNEHFQLWLFTESTLGSLISNDNVIFLQLDDSVNLSVLLRGTLLHQQSKMNVDKMLMPK
EECCCEEEEEEECHHHHHHHCCCCEEEEEECCCCCEEEEEEHHHHHHHHHCCHHHHHCCC
FSTLSDETTAIDCNDIEKYQLKNQITFSAIIRLIDKYLPNNLEHQNEKIQFLCEQVESNN
CCCCCCCCCEECCCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC
PEALRILSHISDNLAYVLMNLINIFSTEKIMLNSPLLQIKHKLFEQIQAKLGKNLLIDNS
CHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCCHHHHHHHHHHHHHHHHCCCEEECCC
VDLVSSQYAWNDPLIACSAIKQGIYEGNLIKDIIQP
HHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7766024; 9097039; 9278503 [H]