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The map label for this gene is bioD [H]

Identifier: 126208096

GI number: 126208096

Start: 700088

End: 700807

Strand: Reverse

Name: bioD [H]

Synonym: APL_0614

Alternate gene names: 126208096

Gene position: 700807-700088 (Counterclockwise)

Preceding gene: 126208097

Following gene: 126208095

Centisome position: 30.81

GC content: 40.42

Gene sequence:

>720_bases
ATGCCTTCATTATTTATTACCGGTACGGATACCAATGTAGGAAAAACAACCGCCACTCGTGCAATTTTACAAACGCTCAC
ACTGCATCATGTCCAAGCGGTAGGCTATAAGCCTATTGCTTGCGGTGGCGACGATTCGTTACCTACGGAACAAAATCAAG
TCGATTATGCCAGTGAAGATAATCCTGATGTTATAACCATTCTCGATAGCTGCCCTTTACCGGTGGAATATCGAGAAATT
AACAGTTATACCTTTATTCATTCCAGCACGCCAGTTTTTGCGGCATTAGATGCGGTTCATCATATTCAAGAAGAAAAATT
AAATAATGACCTCGACCGTTTACAACAAAAATACACTAATATTGTCGTAGAAGGTACGCACGGTTGGCTTACGCCGATTA
ATAAAGACTTAAGTTTTGCCGATTGGGTAAAAGAAAATAATATGCCGGTTATACTGGTTGTCGGGATCAAGGAAGGATGT
GTCAATCACACGTTACTGACGGCACAAGCGATTAAGCAACAAGGGGTTAAATTAGTCGGATGGATTGCGAATAGAGTCAA
TCCGGGATTAAGACATTATGCGGAACTCATTGAATTATTGAGTAAAAAAATTGATGCTCCTCTGCTCGGGCAGATTCCTT
ATATCGGACACCCGTACACCAGAGATCTCACGCCGTATATTCAAAATCCCGAACCGTTATTACAATATTTTAATAAATAA

Upstream 100 bases:

>100_bases
AAGGGATTTATGAAGGTAATTTGATCAAGGACATCATTCAGCCTTAAAAAATCCGTTAAAATATAGGCAATTTTGTAACT
TTTTTGTTAGAGAATAGCAT

Downstream 100 bases:

>100_bases
GACAGAAGCTCCGTTTGATCTGCCCCCCAAAAGTTGGACTAAACCTCCAACGGATTAAGGTGCAGATTTTTTATGACTAA
ATATAACTCGCTATTCAAAC

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase 1; DTBS 1; Dethiobiotin synthase 1 [H]

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MPSLFITGTDTNVGKTTATRAILQTLTLHHVQAVGYKPIACGGDDSLPTEQNQVDYASEDNPDVITILDSCPLPVEYREI
NSYTFIHSSTPVFAALDAVHHIQEEKLNNDLDRLQQKYTNIVVEGTHGWLTPINKDLSFADWVKENNMPVILVVGIKEGC
VNHTLLTAQAIKQQGVKLVGWIANRVNPGLRHYAELIELLSKKIDAPLLGQIPYIGHPYTRDLTPYIQNPEPLLQYFNK

Sequences:

>Translated_239_residues
MPSLFITGTDTNVGKTTATRAILQTLTLHHVQAVGYKPIACGGDDSLPTEQNQVDYASEDNPDVITILDSCPLPVEYREI
NSYTFIHSSTPVFAALDAVHHIQEEKLNNDLDRLQQKYTNIVVEGTHGWLTPINKDLSFADWVKENNMPVILVVGIKEGC
VNHTLLTAQAIKQQGVKLVGWIANRVNPGLRHYAELIELLSKKIDAPLLGQIPYIGHPYTRDLTPYIQNPEPLLQYFNK
>Mature_238_residues
PSLFITGTDTNVGKTTATRAILQTLTLHHVQAVGYKPIACGGDDSLPTEQNQVDYASEDNPDVITILDSCPLPVEYREIN
SYTFIHSSTPVFAALDAVHHIQEEKLNNDLDRLQQKYTNIVVEGTHGWLTPINKDLSFADWVKENNMPVILVVGIKEGCV
NHTLLTAQAIKQQGVKLVGWIANRVNPGLRHYAELIELLSKKIDAPLLGQIPYIGHPYTRDLTPYIQNPEPLLQYFNK

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI87081944, Length=228, Percent_Identity=39.0350877192982, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1786995, Length=213, Percent_Identity=38.0281690140845, Blast_Score=166, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472
- InterPro:   IPR002586 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 26693; Mature: 26562

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSLFITGTDTNVGKTTATRAILQTLTLHHVQAVGYKPIACGGDDSLPTEQNQVDYASED
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCC
NPDVITILDSCPLPVEYREINSYTFIHSSTPVFAALDAVHHIQEEKLNNDLDRLQQKYTN
CCCEEEEECCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHC
IVVEGTHGWLTPINKDLSFADWVKENNMPVILVVGIKEGCVNHTLLTAQAIKQQGVKLVG
EEEECCCCCCCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEE
WIANRVNPGLRHYAELIELLSKKIDAPLLGQIPYIGHPYTRDLTPYIQNPEPLLQYFNK
HHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure 
PSLFITGTDTNVGKTTATRAILQTLTLHHVQAVGYKPIACGGDDSLPTEQNQVDYASED
CCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCC
NPDVITILDSCPLPVEYREINSYTFIHSSTPVFAALDAVHHIQEEKLNNDLDRLQQKYTN
CCCEEEEECCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHC
IVVEGTHGWLTPINKDLSFADWVKENNMPVILVVGIKEGCVNHTLLTAQAIKQQGVKLVG
EEEECCCCCCCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEE
WIANRVNPGLRHYAELIELLSKKIDAPLLGQIPYIGHPYTRDLTPYIQNPEPLLQYFNK
HHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA