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The map label for this gene is pheS

Identifier: 126208090

GI number: 126208090

Start: 694038

End: 695024

Strand: Direct

Name: pheS

Synonym: APL_0608

Alternate gene names: 126208090

Gene position: 694038-695024 (Clockwise)

Preceding gene: 126208088

Following gene: 126208091

Centisome position: 30.51

GC content: 41.84

Gene sequence:

>987_bases
ATGCAACACCTAAAAGAATTAACAGAGAAAGCGAGAAATGCTTTAGATGCCTTAGATCAAGGTTTAGATGCGTTAGAAGA
GTTCCGTGTTGAATATTTCGGTAAGAAAGGTCATTTTACTGCGTTAATGCAAGAATTACGCAATGTTTCGGCGGAAGAGC
GTCCGGCAATCGGTGCGAAAATCAATGAAGCGAAGCAAGCGATTCTTGATATTTTAAATGCGAAAAAAGAAGCATGGGAA
CAAGAAGCATTAAATGCAAAATTAGCGGCAGAAAGTATTGACGTATCATTACCGGGTCGTAAAACCGAATTAGGTGGTTT
ACATCCAGTCTCAATTACGATTGAACGTGTCGTAAAATTCTTCTCTGAATTAGGTTTTACCGTGGCAAGTGGTCCGGAAA
TCGAAACGGATTACTATAACTTTGATGCGTTAAATATTCCTGCTCACCACCCGGCACGTGCGGATCACGATACGTTCTGG
TTCGATGCGCAACGTTTATTACGTACGCAAACGTCAGGCGTACAAATTCGTACCATGGAAAATATGCAGCCGCCAATCCG
TATCGTGGCACCGGGCCGTGTATATCGTAATGACTACGACCAAACACACACACCAATGTTCCACCAAATCGAGTTGCTTT
ATGTGGACAAAAAAGCAAACTTTACCGAGTTAAAAGGCTTGATTCACGATTTCTTAAAAGCGTTTTTCGAAGAAGATTTA
CAAGTGCGTTTCCGTCCATCATTCTTCCCGTTCACAGAGCCTTCGGCTGAAGTGGACGTAATGCGTCAAAACGGTAAATG
GTTAGAAGTATTAGGTTGCGGTATGGTGCATCCTAACGTATTACGCAACGTAGGTATCGATCCGGAAGAATACAGTGGTT
TTGCGGTAGGTATGGGCGTTGAGCGTTTAACGATGTTACGATACAACGTAACGGACTTACGTTCGTTCTTTGAAAATGAC
TTACGTTTCTTAAAACAATTTAAATAA

Upstream 100 bases:

>100_bases
CCTTAAAAATCAACACTTTAGCCTAACTTTACATTTACTTTCTTCAGGCAAAGTATTATCATCACAATAATTTTTCATCA
ATATATTTAGAGGATAGGTA

Downstream 100 bases:

>100_bases
TAGTTAGCTACGGAAATTCACTGAATACACGGGAATGACAAGCGGTCAAAATTAAGTGGAATTTTGCAAATTCCGTGTAA
GAAAATAGAGGATATAAAAT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MQHLKELTEKARNALDALDQGLDALEEFRVEYFGKKGHFTALMQELRNVSAEERPAIGAKINEAKQAILDILNAKKEAWE
QEALNAKLAAESIDVSLPGRKTELGGLHPVSITIERVVKFFSELGFTVASGPEIETDYYNFDALNIPAHHPARADHDTFW
FDAQRLLRTQTSGVQIRTMENMQPPIRIVAPGRVYRNDYDQTHTPMFHQIELLYVDKKANFTELKGLIHDFLKAFFEEDL
QVRFRPSFFPFTEPSAEVDVMRQNGKWLEVLGCGMVHPNVLRNVGIDPEEYSGFAVGMGVERLTMLRYNVTDLRSFFEND
LRFLKQFK

Sequences:

>Translated_328_residues
MQHLKELTEKARNALDALDQGLDALEEFRVEYFGKKGHFTALMQELRNVSAEERPAIGAKINEAKQAILDILNAKKEAWE
QEALNAKLAAESIDVSLPGRKTELGGLHPVSITIERVVKFFSELGFTVASGPEIETDYYNFDALNIPAHHPARADHDTFW
FDAQRLLRTQTSGVQIRTMENMQPPIRIVAPGRVYRNDYDQTHTPMFHQIELLYVDKKANFTELKGLIHDFLKAFFEEDL
QVRFRPSFFPFTEPSAEVDVMRQNGKWLEVLGCGMVHPNVLRNVGIDPEEYSGFAVGMGVERLTMLRYNVTDLRSFFEND
LRFLKQFK
>Mature_328_residues
MQHLKELTEKARNALDALDQGLDALEEFRVEYFGKKGHFTALMQELRNVSAEERPAIGAKINEAKQAILDILNAKKEAWE
QEALNAKLAAESIDVSLPGRKTELGGLHPVSITIERVVKFFSELGFTVASGPEIETDYYNFDALNIPAHHPARADHDTFW
FDAQRLLRTQTSGVQIRTMENMQPPIRIVAPGRVYRNDYDQTHTPMFHQIELLYVDKKANFTELKGLIHDFLKAFFEEDL
QVRFRPSFFPFTEPSAEVDVMRQNGKWLEVLGCGMVHPNVLRNVGIDPEEYSGFAVGMGVERLTMLRYNVTDLRSFFEND
LRFLKQFK

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5729820, Length=318, Percent_Identity=27.9874213836478, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI4758340, Length=257, Percent_Identity=32.295719844358, Blast_Score=114, Evalue=9e-26,
Organism=Escherichia coli, GI1788007, Length=328, Percent_Identity=74.0853658536585, Blast_Score=509, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI32566635, Length=245, Percent_Identity=32.6530612244898, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI32563657, Length=259, Percent_Identity=29.3436293436293, Blast_Score=114, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17508957, Length=259, Percent_Identity=29.3436293436293, Blast_Score=114, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6321087, Length=267, Percent_Identity=28.8389513108614, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6325304, Length=90, Percent_Identity=41.1111111111111, Blast_Score=86, Evalue=8e-18,
Organism=Drosophila melanogaster, GI17137424, Length=292, Percent_Identity=28.7671232876712, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI18858079, Length=260, Percent_Identity=28.0769230769231, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_ACTP2 (A3MZX4)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053315.1
- ProteinModelPortal:   A3MZX4
- SMR:   A3MZX4
- STRING:   A3MZX4
- GeneID:   4850547
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0608
- eggNOG:   COG0016
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- PhylomeDB:   A3MZX4
- ProtClustDB:   PRK00488
- BioCyc:   APLE416269:APL_0608-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 37624; Mature: 37624

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHLKELTEKARNALDALDQGLDALEEFRVEYFGKKGHFTALMQELRNVSAEERPAIGAK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCH
INEAKQAILDILNAKKEAWEQEALNAKLAAESIDVSLPGRKTELGGLHPVSITIERVVKF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCHHHHHHHHHHHH
FSELGFTVASGPEIETDYYNFDALNIPAHHPARADHDTFWFDAQRLLRTQTSGVQIRTME
HHHCCCEECCCCCCCCCEECCCEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECC
NMQPPIRIVAPGRVYRNDYDQTHTPMFHQIELLYVDKKANFTELKGLIHDFLKAFFEEDL
CCCCCEEEEECCHHHHCCCCCCCCCHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCC
QVRFRPSFFPFTEPSAEVDVMRQNGKWLEVLGCGMVHPNVLRNVGIDPEEYSGFAVGMGV
EEEECCCCCCCCCCCCCHHHHHCCCCEEEEEECCCCCHHHHHHCCCCHHHCCCEEEECCH
ERLTMLRYNVTDLRSFFENDLRFLKQFK
HHHHHHHCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQHLKELTEKARNALDALDQGLDALEEFRVEYFGKKGHFTALMQELRNVSAEERPAIGAK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCH
INEAKQAILDILNAKKEAWEQEALNAKLAAESIDVSLPGRKTELGGLHPVSITIERVVKF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCHHHHHHHHHHHH
FSELGFTVASGPEIETDYYNFDALNIPAHHPARADHDTFWFDAQRLLRTQTSGVQIRTME
HHHCCCEECCCCCCCCCEECCCEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECC
NMQPPIRIVAPGRVYRNDYDQTHTPMFHQIELLYVDKKANFTELKGLIHDFLKAFFEEDL
CCCCCEEEEECCHHHHCCCCCCCCCHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCC
QVRFRPSFFPFTEPSAEVDVMRQNGKWLEVLGCGMVHPNVLRNVGIDPEEYSGFAVGMGV
EEEECCCCCCCCCCCCCHHHHHCCCCEEEEEECCCCCHHHHHHCCCCHHHCCCEEEECCH
ERLTMLRYNVTDLRSFFENDLRFLKQFK
HHHHHHHCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA