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The map label for this gene is pnp

Identifier: 126208059

GI number: 126208059

Start: 650506

End: 652653

Strand: Reverse

Name: pnp

Synonym: APL_0577

Alternate gene names: 126208059

Gene position: 652653-650506 (Counterclockwise)

Preceding gene: 126208065

Following gene: 126208058

Centisome position: 28.69

GC content: 45.76

Gene sequence:

>2148_bases
ATGAATCCAATTGTTAAGCAATTTAAATACGGTCAGCATACCGTAACGTTAGAAACAGGTGCTATCGCACGTCAAGCAAC
GGCGGCCGTTATGGCAAGCATGGACGATACCACCGTATTCGTCACCGTTGTGGCTAAAAAAGAGGTTAAAGAAGGTCAAG
ATTTCTTCCCATTAACGGTAGATTATCAAGAGCGTACTTATGCAGCCGGTCGTATTCCCGGCGGTTTCTTCAAACGTGAA
GGTCGTCCGTCAGAAGGCGAAACATTAATCGCTCGTTTAATCGACCGTCCTGTGCGCCCGCTTTTCCCGGAAGGTTTCTT
TAACGAAATTCAAGTGATTGCTACGGTTGTTTCGGTGAACCCGCAAATCAGCCCGGATTTAGTGGCGATGATCGGTGCTT
CAGCGGCGCTTTCATTATCAGGCGTACCGTTCAACGGTCCTATCGGTGCGGCGCGTGTCGGTTTTATCAATGATCAATTC
GTATTAAACCCGACCACTTCAGAACAAAAAATCAGCCGCTTAGACCTTGTAGTAGCAGGGACGGATAAAGCGGTATTAAT
GGTTGAATCGGAAGCGGATATTCTTTCTGAAGAACAAATGTTATCCGCAGTCGTATTCGGTCATCAGCAACAACAAGTTG
TAATTGAAAATATTAAAGAATTCGTAAAAGAAGCGGGTAAACCGCGTTGGGATTGGGTTGCGCCGGAACCGAATACCGCA
TTAATCAACCAAGTTAAAGCGTTAGCGGAAGCGCGTATCGGTGATGCGTATCGTATCACTGAAAAACAAGCGCGTTACGA
ACAAATCGATGCAATTAAAGCGGATGTTATCGCACAATTAACCGCACAAGACGAAACCGTTTCGGAAGGTGCGATTATCG
ATATTATTACCGCATTAGAAAGTTCTATCGTGCGTGGTCGTATTATTGCCGGCGAACCGCGTATTGACGGTCGTACGGTA
GATACGGTTCGTGCATTAGACATTTGCACCGGCGTATTACCTCGTACGCACGGTTCTGCAATCTTTACTCGCGGTGAAAC
ACAAGCATTAGCGGTTGCAACCTTAGGTACTGAGCGCGATGCACAAATTATTGACGAATTAACCGGCGAGAAATCAGACC
GTTTCTTATTCCACTATAACTTCCCTCCGTACTCTGTCGGTGAAACGGGTCGTATCGGTTCGCCGAAACGTCGTGAAATC
GGCCACGGTCGTTTAGCGAAACGCGGTGTATTAGCGGTAATGCCGACTGCGGAAGAATTCCCGTATGTAGTGCGCGTAGT
ATCTGAAATTACCGAATCAAACGGTTCTTCTTCAATGGCTTCCGTATGCGGCGCATCTTTAGCGTTAATGGACGCAGGCG
TACCGATTAAAGCGGCGGTTGCGGGTATCGCAATGGGCTTAGTGAAAGAAGAAGAAAAATTTGTGGTGCTTTCAGACATC
TTAGGTGACGAAGACCATTTAGGCGATATGGACTTCAAAGTAGCCGGTACGCGTGAAGGTGTAACCGCACTTCAAATGGA
TATTAAAATCGAAGGTATCACGCCGGAAATTATGCAAATCGCATTAAATCAAGCGAAAGGTGCGCGTATGCACATCTTAA
GCGTGATGGAACAAGCGATTCCTGCACCTCGTGCCGATATTTCCGATTTTGCGCCTCGTATTCATACGATGAAAATCGAT
CCGAAGAAAATCAAAGACGTTATCGGTAAAGGTGGTGCGGTAATTCGTGCATTAACCGAAGAAACCGGTACTTCGATTGA
TATTGATGACGACGGTACCGTAAAAATCGCTGCAACCGATAATAATGCGGCGAAAGCGGTAATGGCTCGTATCGAAGATA
TCGTGGCTGAAGTTGAAGTGAACGCGATCTACAAAGGTAAAGTAACTCGCGTTGTGGACTTCGGTGCGTTCGTTTCTATC
TTAGGTGGTAAAGAAGGTTTAGTACATATTTCGCAAATCACGAATGAGCGTGTCGAACGCGTTGCGGATTACTTATCCGT
AGGTCAAGAAGTGACTGTGAAAGTAGTTGAAATCGATCGTCAAAACCGTATCCGTTTAACTATGAAAGATCTTAATAACG
ATACCCCTGTTGCCGAAAATGTAACTGAAGAAGCGGAAGTTTCGTCAGAACAACAAGCTGAGATTTAA

Upstream 100 bases:

>100_bases
AATATAAAATGAAGAAAATAAGATTAATCAGAGAATTTTTGCTAGTCGCATTAAAAGAAATAAAACAATAAATAAAGTAC
AAATTAATAGGAATGTCAAA

Downstream 100 bases:

>100_bases
AAATAAGATATCTACCTTCAATTCTTGAAGGTAGATATCTTCTCATAGGCGATTTACCTAATGTGTTTACTGATTTTTAA
AGTGTTTCACCTTCGCTTCT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 715; Mature: 715

Protein sequence:

>715_residues
MNPIVKQFKYGQHTVTLETGAIARQATAAVMASMDDTTVFVTVVAKKEVKEGQDFFPLTVDYQERTYAAGRIPGGFFKRE
GRPSEGETLIARLIDRPVRPLFPEGFFNEIQVIATVVSVNPQISPDLVAMIGASAALSLSGVPFNGPIGAARVGFINDQF
VLNPTTSEQKISRLDLVVAGTDKAVLMVESEADILSEEQMLSAVVFGHQQQQVVIENIKEFVKEAGKPRWDWVAPEPNTA
LINQVKALAEARIGDAYRITEKQARYEQIDAIKADVIAQLTAQDETVSEGAIIDIITALESSIVRGRIIAGEPRIDGRTV
DTVRALDICTGVLPRTHGSAIFTRGETQALAVATLGTERDAQIIDELTGEKSDRFLFHYNFPPYSVGETGRIGSPKRREI
GHGRLAKRGVLAVMPTAEEFPYVVRVVSEITESNGSSSMASVCGASLALMDAGVPIKAAVAGIAMGLVKEEEKFVVLSDI
LGDEDHLGDMDFKVAGTREGVTALQMDIKIEGITPEIMQIALNQAKGARMHILSVMEQAIPAPRADISDFAPRIHTMKID
PKKIKDVIGKGGAVIRALTEETGTSIDIDDDGTVKIAATDNNAAKAVMARIEDIVAEVEVNAIYKGKVTRVVDFGAFVSI
LGGKEGLVHISQITNERVERVADYLSVGQEVTVKVVEIDRQNRIRLTMKDLNNDTPVAENVTEEAEVSSEQQAEI

Sequences:

>Translated_715_residues
MNPIVKQFKYGQHTVTLETGAIARQATAAVMASMDDTTVFVTVVAKKEVKEGQDFFPLTVDYQERTYAAGRIPGGFFKRE
GRPSEGETLIARLIDRPVRPLFPEGFFNEIQVIATVVSVNPQISPDLVAMIGASAALSLSGVPFNGPIGAARVGFINDQF
VLNPTTSEQKISRLDLVVAGTDKAVLMVESEADILSEEQMLSAVVFGHQQQQVVIENIKEFVKEAGKPRWDWVAPEPNTA
LINQVKALAEARIGDAYRITEKQARYEQIDAIKADVIAQLTAQDETVSEGAIIDIITALESSIVRGRIIAGEPRIDGRTV
DTVRALDICTGVLPRTHGSAIFTRGETQALAVATLGTERDAQIIDELTGEKSDRFLFHYNFPPYSVGETGRIGSPKRREI
GHGRLAKRGVLAVMPTAEEFPYVVRVVSEITESNGSSSMASVCGASLALMDAGVPIKAAVAGIAMGLVKEEEKFVVLSDI
LGDEDHLGDMDFKVAGTREGVTALQMDIKIEGITPEIMQIALNQAKGARMHILSVMEQAIPAPRADISDFAPRIHTMKID
PKKIKDVIGKGGAVIRALTEETGTSIDIDDDGTVKIAATDNNAAKAVMARIEDIVAEVEVNAIYKGKVTRVVDFGAFVSI
LGGKEGLVHISQITNERVERVADYLSVGQEVTVKVVEIDRQNRIRLTMKDLNNDTPVAENVTEEAEVSSEQQAEI
>Mature_715_residues
MNPIVKQFKYGQHTVTLETGAIARQATAAVMASMDDTTVFVTVVAKKEVKEGQDFFPLTVDYQERTYAAGRIPGGFFKRE
GRPSEGETLIARLIDRPVRPLFPEGFFNEIQVIATVVSVNPQISPDLVAMIGASAALSLSGVPFNGPIGAARVGFINDQF
VLNPTTSEQKISRLDLVVAGTDKAVLMVESEADILSEEQMLSAVVFGHQQQQVVIENIKEFVKEAGKPRWDWVAPEPNTA
LINQVKALAEARIGDAYRITEKQARYEQIDAIKADVIAQLTAQDETVSEGAIIDIITALESSIVRGRIIAGEPRIDGRTV
DTVRALDICTGVLPRTHGSAIFTRGETQALAVATLGTERDAQIIDELTGEKSDRFLFHYNFPPYSVGETGRIGSPKRREI
GHGRLAKRGVLAVMPTAEEFPYVVRVVSEITESNGSSSMASVCGASLALMDAGVPIKAAVAGIAMGLVKEEEKFVVLSDI
LGDEDHLGDMDFKVAGTREGVTALQMDIKIEGITPEIMQIALNQAKGARMHILSVMEQAIPAPRADISDFAPRIHTMKID
PKKIKDVIGKGGAVIRALTEETGTSIDIDDDGTVKIAATDNNAAKAVMARIEDIVAEVEVNAIYKGKVTRVVDFGAFVSI
LGGKEGLVHISQITNERVERVADYLSVGQEVTVKVVEIDRQNRIRLTMKDLNNDTPVAENVTEEAEVSSEQQAEI

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=707, Percent_Identity=37.1994342291372, Blast_Score=433, Evalue=1e-121,
Organism=Escherichia coli, GI145693187, Length=691, Percent_Identity=77.7134587554269, Blast_Score=1117, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=705, Percent_Identity=32.4822695035461, Blast_Score=337, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI17535281, Length=80, Percent_Identity=46.25, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI281362905, Length=692, Percent_Identity=37.7167630057803, Blast_Score=451, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24651641, Length=692, Percent_Identity=37.7167630057803, Blast_Score=451, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24651643, Length=692, Percent_Identity=37.7167630057803, Blast_Score=451, Evalue=1e-127,
Organism=Drosophila melanogaster, GI161079377, Length=633, Percent_Identity=37.4407582938389, Blast_Score=412, Evalue=1e-115,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_ACTP2 (A3MZU3)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053284.1
- ProteinModelPortal:   A3MZU3
- SMR:   A3MZU3
- STRING:   A3MZU3
- GeneID:   4849054
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0577
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- PhylomeDB:   A3MZU3
- ProtClustDB:   PRK11824
- BioCyc:   APLE416269:APL_0577-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 77398; Mature: 77398

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPIVKQFKYGQHTVTLETGAIARQATAAVMASMDDTTVFVTVVAKKEVKEGQDFFPLTV
CCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEEEHHHHHCCCCCCEEEE
DYQERTYAAGRIPGGFFKREGRPSEGETLIARLIDRPVRPLFPEGFFNEIQVIATVVSVN
ECCCCEEECCCCCCHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCC
PQISPDLVAMIGASAALSLSGVPFNGPIGAARVGFINDQFVLNPTTSEQKISRLDLVVAG
CCCCHHHHHHHCCCHHEEECCCCCCCCCCCEEEEEECCEEEECCCCCHHHHHHEEEEEEC
TDKAVLMVESEADILSEEQMLSAVVFGHQQQQVVIENIKEFVKEAGKPRWDWVAPEPNTA
CCCEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
LINQVKALAEARIGDAYRITEKQARYEQIDAIKADVIAQLTAQDETVSEGAIIDIITALE
HHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
SSIVRGRIIAGEPRIDGRTVDTVRALDICTGVLPRTHGSAIFTRGETQALAVATLGTERD
HHHHCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEECCCCCH
AQIIDELTGEKSDRFLFHYNFPPYSVGETGRIGSPKRREIGHGRLAKRGVLAVMPTAEEF
HHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHCCCCCHHHCCEEEEECCHHHH
PYVVRVVSEITESNGSSSMASVCGASLALMDAGVPIKAAVAGIAMGLVKEEEKFVVLSDI
HHHHHHHHHHHHCCCCHHHHHHHHHHHEEHCCCCCHHHHHHHHHHHHHCCCCCEEEEHHH
LGDEDHLGDMDFKVAGTREGVTALQMDIKIEGITPEIMQIALNQAKGARMHILSVMEQAI
CCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHC
PAPRADISDFAPRIHTMKIDPKKIKDVIGKGGAVIRALTEETGTSIDIDDDGTVKIAATD
CCCCCCHHHHCCCEEEEECCHHHHHHHHCCCCCEEEEEHHCCCCEEEECCCCEEEEEECC
NNAAKAVMARIEDIVAEVEVNAIYKGKVTRVVDFGAFVSILGGKEGLVHISQITNERVER
CCHHHHHHHHHHHHHHHHEEEEEECCCEEEEEHHHHHHHHHCCCCCEEEHHHHHHHHHHH
VADYLSVGQEVTVKVVEIDRQNRIRLTMKDLNNDTPVAENVTEEAEVSSEQQAEI
HHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCCCCCHHCCCHHHHCCCHHHCCC
>Mature Secondary Structure
MNPIVKQFKYGQHTVTLETGAIARQATAAVMASMDDTTVFVTVVAKKEVKEGQDFFPLTV
CCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEEEHHHHHCCCCCCEEEE
DYQERTYAAGRIPGGFFKREGRPSEGETLIARLIDRPVRPLFPEGFFNEIQVIATVVSVN
ECCCCEEECCCCCCHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCC
PQISPDLVAMIGASAALSLSGVPFNGPIGAARVGFINDQFVLNPTTSEQKISRLDLVVAG
CCCCHHHHHHHCCCHHEEECCCCCCCCCCCEEEEEECCEEEECCCCCHHHHHHEEEEEEC
TDKAVLMVESEADILSEEQMLSAVVFGHQQQQVVIENIKEFVKEAGKPRWDWVAPEPNTA
CCCEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
LINQVKALAEARIGDAYRITEKQARYEQIDAIKADVIAQLTAQDETVSEGAIIDIITALE
HHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
SSIVRGRIIAGEPRIDGRTVDTVRALDICTGVLPRTHGSAIFTRGETQALAVATLGTERD
HHHHCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEECCCCCH
AQIIDELTGEKSDRFLFHYNFPPYSVGETGRIGSPKRREIGHGRLAKRGVLAVMPTAEEF
HHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHCCCCCHHHCCEEEEECCHHHH
PYVVRVVSEITESNGSSSMASVCGASLALMDAGVPIKAAVAGIAMGLVKEEEKFVVLSDI
HHHHHHHHHHHHCCCCHHHHHHHHHHHEEHCCCCCHHHHHHHHHHHHHCCCCCEEEEHHH
LGDEDHLGDMDFKVAGTREGVTALQMDIKIEGITPEIMQIALNQAKGARMHILSVMEQAI
CCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHC
PAPRADISDFAPRIHTMKIDPKKIKDVIGKGGAVIRALTEETGTSIDIDDDGTVKIAATD
CCCCCCHHHHCCCEEEEECCHHHHHHHHCCCCCEEEEEHHCCCCEEEECCCCEEEEEECC
NNAAKAVMARIEDIVAEVEVNAIYKGKVTRVVDFGAFVSILGGKEGLVHISQITNERVER
CCHHHHHHHHHHHHHHHHEEEEEECCCEEEEEHHHHHHHHHCCCCCEEEHHHHHHHHHHH
VADYLSVGQEVTVKVVEIDRQNRIRLTMKDLNNDTPVAENVTEEAEVSSEQQAEI
HHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCCCCCHHCCCHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA