Click here to switch to the map view.

The map label for this gene is pdxT

Identifier: 126208055

GI number: 126208055

Start: 646167

End: 646742

Strand: Direct

Name: pdxT

Synonym: APL_0573

Alternate gene names: 126208055

Gene position: 646167-646742 (Clockwise)

Preceding gene: 126208054

Following gene: 126208060

Centisome position: 28.41

GC content: 43.92

Gene sequence:

>576_bases
ATGAACGATTATACAAAATATACCATAGGCGTATTGTCATTACAGGGGGCGGTTTCGGAACATATCGCTCAAATAGAAAC
TTTGGGCGCAAAAGCCATAGCGGTAAAGTCGTTATCCGAATTGCAGCAAGTTGATGCTTTGGTGTTACCCGGCGGTGAAA
GTACCGCAATGAGGCGCTTAATGCATTCAAGCGGTTTATTTCAGGCACTCAAATCATTTGATAAGCCGATATTAGGTACT
TGTGCCGGATTGATTCTGTTAGCGAATAAGCTTGAAGGCGGTGAGCCTCCTCATTTAGCCAAAATGAATATTCAAGTTCA
GCGTAACGCTTTCGGTCGGCAGGTTGATAGTTTCCAAACGGACTTGATGATAAAAGGATTTGCTGATTCGTTTCCGGCGG
TTTTTATTCGCGCACCTTATATCAGTCGTATAGGCAGTGAGGTAGAAGTGCTGGCGGAATGGCAGGGCAATGTTGTATTT
GCCAAGCAAGGCAATTTACTGGCTTGTGCGTTTCATCCGGAATTAACATCGGATACGCGTGTTGTAGAATTGTTTCTACA
ACAGCTAAAAGAGTAG

Upstream 100 bases:

>100_bases
ACTTAATCGCTCGTCTTTCGGCGGATTTAGGCGAACCGATGAGAGGGTTGGAAATCAGCGAATTAGCGGTACAAGATCGT
ATGCAAGAGCGTGGTTGGTA

Downstream 100 bases:

>100_bases
TTTTTATCGTAAATCTAAGGACATCATTTAGGTGTCCTTAGATCGAATTGCATTTTGCAAAAAATCGAGGAAATTAGACC
GCTTGCTTCTGCCGTTTTTG

Product: glutamine amidotransferase subunit PdxT

Products: NA

Alternate protein names: Glutamine amidotransferase glutaminase subunit pdxT

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MNDYTKYTIGVLSLQGAVSEHIAQIETLGAKAIAVKSLSELQQVDALVLPGGESTAMRRLMHSSGLFQALKSFDKPILGT
CAGLILLANKLEGGEPPHLAKMNIQVQRNAFGRQVDSFQTDLMIKGFADSFPAVFIRAPYISRIGSEVEVLAEWQGNVVF
AKQGNLLACAFHPELTSDTRVVELFLQQLKE

Sequences:

>Translated_191_residues
MNDYTKYTIGVLSLQGAVSEHIAQIETLGAKAIAVKSLSELQQVDALVLPGGESTAMRRLMHSSGLFQALKSFDKPILGT
CAGLILLANKLEGGEPPHLAKMNIQVQRNAFGRQVDSFQTDLMIKGFADSFPAVFIRAPYISRIGSEVEVLAEWQGNVVF
AKQGNLLACAFHPELTSDTRVVELFLQQLKE
>Mature_191_residues
MNDYTKYTIGVLSLQGAVSEHIAQIETLGAKAIAVKSLSELQQVDALVLPGGESTAMRRLMHSSGLFQALKSFDKPILGT
CAGLILLANKLEGGEPPHLAKMNIQVQRNAFGRQVDSFQTDLMIKGFADSFPAVFIRAPYISRIGSEVEVLAEWQGNVVF
AKQGNLLACAFHPELTSDTRVVELFLQQLKE

Specific function: Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to pdxS

COG id: COG0311

COG function: function code H; Predicted glutamine amidotransferase involved in pyridoxine biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine amidotransferase pdxT/SNO family

Homologues:

Organism=Saccharomyces cerevisiae, GI6323742, Length=214, Percent_Identity=38.3177570093458, Blast_Score=124, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6323995, Length=211, Percent_Identity=36.4928909952607, Blast_Score=114, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6321048, Length=211, Percent_Identity=36.9668246445498, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXT_ACTP2 (A3MZT9)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053280.1
- ProteinModelPortal:   A3MZT9
- SMR:   A3MZT9
- STRING:   A3MZT9
- GeneID:   4848573
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0573
- eggNOG:   COG0311
- HOGENOM:   HBG292341
- OMA:   QGDVREH
- PhylomeDB:   A3MZT9
- ProtClustDB:   PRK13525
- BioCyc:   APLE416269:APL_0573-MONOMER
- HAMAP:   MF_01615
- InterPro:   IPR002161
- InterPro:   IPR021196
- PIRSF:   PIRSF005639
- TIGRFAMs:   TIGR03800

Pfam domain/function: PF01174 SNO

EC number: NA

Molecular weight: Translated: 20832; Mature: 20832

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01236 PDXT_SNO_1; PS51130 PDXT_SNO_2

Important sites: ACT_SITE 81-81 ACT_SITE 172-172 ACT_SITE 174-174 BINDING 108-108

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDYTKYTIGVLSLQGAVSEHIAQIETLGAKAIAVKSLSELQQVDALVLPGGESTAMRRL
CCCCHHHEEEEHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCEEEECCCCHHHHHHH
MHSSGLFQALKSFDKPILGTCAGLILLANKLEGGEPPHLAKMNIQVQRNAFGRQVDSFQT
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCHHHHHHHHHHH
DLMIKGFADSFPAVFIRAPYISRIGSEVEVLAEWQGNVVFAKQGNLLACAFHPELTSDTR
HHEEECCCCCCCEEEEECHHHHHCCCHHEEEEECCCCEEEEECCCEEEEEECCCCCCCHH
VVELFLQQLKE
HHHHHHHHHCC
>Mature Secondary Structure
MNDYTKYTIGVLSLQGAVSEHIAQIETLGAKAIAVKSLSELQQVDALVLPGGESTAMRRL
CCCCHHHEEEEHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCEEEECCCCHHHHHHH
MHSSGLFQALKSFDKPILGTCAGLILLANKLEGGEPPHLAKMNIQVQRNAFGRQVDSFQT
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCHHHHHHHHHHH
DLMIKGFADSFPAVFIRAPYISRIGSEVEVLAEWQGNVVFAKQGNLLACAFHPELTSDTR
HHEEECCCCCCCEEEEECHHHHHCCCHHEEEEECCCCEEEEECCCEEEEEECCCCCCCHH
VVELFLQQLKE
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA