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The map label for this gene is thiH [H]

Identifier: 126208018

GI number: 126208018

Start: 607036

End: 608181

Strand: Direct

Name: thiH [H]

Synonym: APL_0536

Alternate gene names: 126208018

Gene position: 607036-608181 (Clockwise)

Preceding gene: 126208017

Following gene: 126208019

Centisome position: 26.69

GC content: 43.54

Gene sequence:

>1146_bases
ATGAAAACAAATCAGTTTGCGCATTTTAGCGATTATTGGCAAACGCTCGATTGGGGACATCTCGCTTTATCGTTAAGAGC
AAAAACTTCAGCGGACGTAGAGAATGCGTTGCAAAGTTCAAACCGAGATTTAAATGACTTTATGGCATTAATCTCGCCGG
CGGCGGAAGCCTATTTAGAACCGATGGCACAGCTGGCTCAACAGCTTACTCGTCAGCGTTTCGGTAATACGGTCGGCTTT
TATCTACCGCTTTATCTCTCTAATGTATGTACCAATGACTGTACTTATTGCGGTTTTTCAATGAGTAACCGCATTAAGCG
TAAGATTCTTGACGAGCAAGAAATTCTCAATGAAATCAAAGCGATTAAGTCGATGGGCTACGAGCATTTATTACTGGTGA
CCGGCGAACACGAAACCAAAGTCGGTATGGCTTATTTTAGAGAAAAATTAAAACTGATCCGCCCGCATTTTTCCGCCTTA
TTAATGGAAGTGCAACCGCTTCAGTCTGAGGAATATGTCGAATTAAAAACACTCGGTTTGGACGGCGTACTGGTTTATCA
AGAAACCTATAATGCCGCCGAATATGCCAAACATCATTTACGCGGTCAGAAACAAGATTTTATTTACCGTTTGGACAGCC
AAGATCGTTTAGGGCAAGCGGGCATAGATAAAATCGGTTTAGGTGCATTAATCGGGCTTTCAAATGACTGGCGTACCGAT
ATTTACTTTGTCGGCGAGCATTTGAATTACCTGCAAAATAAATATTGGCAAAGCCGTTATTCGATTTCATTTCCTCGTTT
ACGTCCTTGTGTCGGCGGTTTGCAGCCGGCTTCGCAAATGAGCGATAAGCAGTTGCTGCAAATTATTTGTGCATTCCGTC
TTTGTTTCCCGGAAGTAGAACTTTCGCTTTCGACTCGTGAAAGTGCCAATTTCCGCAATAACGTGATTCCGATTGCGATT
AATACCATCAGCGCAGGCTCGAAAACACAGCCGGGCGGCTATGCGGAAGAAGACGATAAACATCTCGAACAATTCTCACC
GGATGATGAGCGTCCTCCGCAAATTATTGCTGCCGAATTGGTTAAACGGGGCTTACAACCGGTTTGGAAAGATTGGGAAC
CGTATTTAGGTAGAACGTTATCTTAA

Upstream 100 bases:

>100_bases
GGAAGCGGGGCGTTTAGGTTATTTGGCAGGGCTTGGCAAAGTAAGTCAAACTGCACAGGCAACCAGCCCATTAACCGGCT
TTTTAGGCTAGGGAGTTGCA

Downstream 100 bases:

>100_bases
ATGATATGGTTAAAAAAGGAGAAAAAATGACCGCTATTCAGCAATTTATCCAAAATGCAGAGCACTTACTGGCGTACATA
TACCGAACATCTTTTTGTGC

Product: thiazole biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MKTNQFAHFSDYWQTLDWGHLALSLRAKTSADVENALQSSNRDLNDFMALISPAAEAYLEPMAQLAQQLTRQRFGNTVGF
YLPLYLSNVCTNDCTYCGFSMSNRIKRKILDEQEILNEIKAIKSMGYEHLLLVTGEHETKVGMAYFREKLKLIRPHFSAL
LMEVQPLQSEEYVELKTLGLDGVLVYQETYNAAEYAKHHLRGQKQDFIYRLDSQDRLGQAGIDKIGLGALIGLSNDWRTD
IYFVGEHLNYLQNKYWQSRYSISFPRLRPCVGGLQPASQMSDKQLLQIICAFRLCFPEVELSLSTRESANFRNNVIPIAI
NTISAGSKTQPGGYAEEDDKHLEQFSPDDERPPQIIAAELVKRGLQPVWKDWEPYLGRTLS

Sequences:

>Translated_381_residues
MKTNQFAHFSDYWQTLDWGHLALSLRAKTSADVENALQSSNRDLNDFMALISPAAEAYLEPMAQLAQQLTRQRFGNTVGF
YLPLYLSNVCTNDCTYCGFSMSNRIKRKILDEQEILNEIKAIKSMGYEHLLLVTGEHETKVGMAYFREKLKLIRPHFSAL
LMEVQPLQSEEYVELKTLGLDGVLVYQETYNAAEYAKHHLRGQKQDFIYRLDSQDRLGQAGIDKIGLGALIGLSNDWRTD
IYFVGEHLNYLQNKYWQSRYSISFPRLRPCVGGLQPASQMSDKQLLQIICAFRLCFPEVELSLSTRESANFRNNVIPIAI
NTISAGSKTQPGGYAEEDDKHLEQFSPDDERPPQIIAAELVKRGLQPVWKDWEPYLGRTLS
>Mature_381_residues
MKTNQFAHFSDYWQTLDWGHLALSLRAKTSADVENALQSSNRDLNDFMALISPAAEAYLEPMAQLAQQLTRQRFGNTVGF
YLPLYLSNVCTNDCTYCGFSMSNRIKRKILDEQEILNEIKAIKSMGYEHLLLVTGEHETKVGMAYFREKLKLIRPHFSAL
LMEVQPLQSEEYVELKTLGLDGVLVYQETYNAAEYAKHHLRGQKQDFIYRLDSQDRLGQAGIDKIGLGALIGLSNDWRTD
IYFVGEHLNYLQNKYWQSRYSISFPRLRPCVGGLQPASQMSDKQLLQIICAFRLCFPEVELSLSTRESANFRNNVIPIAI
NTISAGSKTQPGGYAEEDDKHLEQFSPDDERPPQIIAAELVKRGLQPVWKDWEPYLGRTLS

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=371, Percent_Identity=67.1159029649596, Blast_Score=530, Evalue=1e-152,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 43549; Mature: 43549

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTNQFAHFSDYWQTLDWGHLALSLRAKTSADVENALQSSNRDLNDFMALISPAAEAYLE
CCCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
PMAQLAQQLTRQRFGNTVGFYLPLYLSNVCTNDCTYCGFSMSNRIKRKILDEQEILNEIK
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHH
AIKSMGYEHLLLVTGEHETKVGMAYFREKLKLIRPHFSALLMEVQPLQSEEYVELKTLGL
HHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECC
DGVLVYQETYNAAEYAKHHLRGQKQDFIYRLDSQDRLGQAGIDKIGLGALIGLSNDWRTD
CEEEEEHHHCCHHHHHHHHHCCCCCHHEEEECCCCCCCCCCCCHHCCHHEEECCCCCCCH
IYFVGEHLNYLQNKYWQSRYSISFPRLRPCVGGLQPASQMSDKQLLQIICAFRLCFPEVE
HEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHCCHHH
LSLSTRESANFRNNVIPIAINTISAGSKTQPGGYAEEDDKHLEQFSPDDERPPQIIAAEL
EEECCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHH
VKRGLQPVWKDWEPYLGRTLS
HHHCCCHHHHCCHHHCCCCCC
>Mature Secondary Structure
MKTNQFAHFSDYWQTLDWGHLALSLRAKTSADVENALQSSNRDLNDFMALISPAAEAYLE
CCCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
PMAQLAQQLTRQRFGNTVGFYLPLYLSNVCTNDCTYCGFSMSNRIKRKILDEQEILNEIK
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHH
AIKSMGYEHLLLVTGEHETKVGMAYFREKLKLIRPHFSALLMEVQPLQSEEYVELKTLGL
HHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECC
DGVLVYQETYNAAEYAKHHLRGQKQDFIYRLDSQDRLGQAGIDKIGLGALIGLSNDWRTD
CEEEEEHHHCCHHHHHHHHHCCCCCHHEEEECCCCCCCCCCCCHHCCHHEEECCCCCCCH
IYFVGEHLNYLQNKYWQSRYSISFPRLRPCVGGLQPASQMSDKQLLQIICAFRLCFPEVE
HEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHCCHHH
LSLSTRESANFRNNVIPIAINTISAGSKTQPGGYAEEDDKHLEQFSPDDERPPQIIAAEL
EEECCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHH
VKRGLQPVWKDWEPYLGRTLS
HHHCCCHHHHCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 10082377 [H]