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The map label for this gene is thiG [H]

Identifier: 126208017

GI number: 126208017

Start: 606616

End: 607026

Strand: Direct

Name: thiG [H]

Synonym: APL_0535

Alternate gene names: 126208017

Gene position: 606616-607026 (Clockwise)

Preceding gene: 126208016

Following gene: 126208018

Centisome position: 26.67

GC content: 49.88

Gene sequence:

>411_bases
ATGTTGGTGAAAGAAGGTTTTATCGTATTGCCTTATTGCGGTGCGGATCCGGTGTTATGTAAACGCTTGGAGGAAGTCGG
TTGTGCGGCGGTGATGCCGTTAGGGGCGCCGATTGGAACCAACCAAGGGCTAAAAACTCAAGATTTTTTACGCATTATTA
TTGAACAAAGCCGAGTGCCGGTAGTAGTGGATGCCGGTATCGGCGCACCGAGCCACGCAGCACAAGCAATGGAAATGGGT
GCGGATGCGGTATTAGTTAATACGGCAATGACGGTAGCCCGTGATCCGATTCAAATTGCACAAGCCTTTAAATTAGCGGT
GGAAGCGGGGCGTTTAGGTTATTTGGCAGGGCTTGGCAAAGTAAGTCAAACTGCACAGGCAACCAGCCCATTAACCGGCT
TTTTAGGCTAG

Upstream 100 bases:

>100_bases
GGCACGAGAAGCATTACAGACGAACTGGATCAAACTTGAAATTCACCCTGATGTTCGTTATTTGCTGCCGGATCCGATTG
AAACGCTAAAAGGTGCGGAA

Downstream 100 bases:

>100_bases
GGAGTTGCAATGAAAACAAATCAGTTTGCGCATTTTAGCGATTATTGGCAAACGCTCGATTGGGGACATCTCGCTTTATC
GTTAAGAGCAAAAACTTCAG

Product: thiazole biosynthesis protein ThiG

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 136; Mature: 136

Protein sequence:

>136_residues
MLVKEGFIVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGTNQGLKTQDFLRIIIEQSRVPVVVDAGIGAPSHAAQAMEMG
ADAVLVNTAMTVARDPIQIAQAFKLAVEAGRLGYLAGLGKVSQTAQATSPLTGFLG

Sequences:

>Translated_136_residues
MLVKEGFIVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGTNQGLKTQDFLRIIIEQSRVPVVVDAGIGAPSHAAQAMEMG
ADAVLVNTAMTVARDPIQIAQAFKLAVEAGRLGYLAGLGKVSQTAQATSPLTGFLG
>Mature_136_residues
MLVKEGFIVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGTNQGLKTQDFLRIIIEQSRVPVVVDAGIGAPSHAAQAMEMG
ADAVLVNTAMTVARDPIQIAQAFKLAVEAGRLGYLAGLGKVSQTAQATSPLTGFLG

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI48994993, Length=134, Percent_Identity=75.3731343283582, Blast_Score=206, Evalue=4e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR008867 [H]

Pfam domain/function: PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 14049; Mature: 14049

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVKEGFIVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGTNQGLKTQDFLRIIIEQSRVP
CEECCCCEEEEECCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCC
VVVDAGIGAPSHAAQAMEMGADAVLVNTAMTVARDPIQIAQAFKLAVEAGRLGYLAGLGK
EEEECCCCCCHHHHHHHHCCCCEEEEHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHCCH
VSQTAQATSPLTGFLG
HHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLVKEGFIVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGTNQGLKTQDFLRIIIEQSRVP
CEECCCCEEEEECCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCC
VVVDAGIGAPSHAAQAMEMGADAVLVNTAMTVARDPIQIAQAFKLAVEAGRLGYLAGLGK
EEEECCCCCCHHHHHHHHCCCCEEEEHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHCCH
VSQTAQATSPLTGFLG
HHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA