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The map label for this gene is thiF [H]

Identifier: 126208015

GI number: 126208015

Start: 605569

End: 605949

Strand: Direct

Name: thiF [H]

Synonym: APL_0533

Alternate gene names: 126208015

Gene position: 605569-605949 (Clockwise)

Preceding gene: 126208014

Following gene: 126208016

Centisome position: 26.62

GC content: 45.93

Gene sequence:

>381_bases
GTGTTGGATTGCTCGGACAATATGGCAACACGTCACGCAGTTAATAAAGTGTGTGTGGAAAACCAAATTCCGCTGATTTC
CGGTTCGGCGGTCGGTATGGACGGGCAATTATTGGGCGTGTTTCCGCCTTATCAACACGGCTGCTACGCCTGCCTATTTT
CCGATACTGAGATCGGTAATCTAAATTGTCGCACCGCCGGTGTTTTAGCACCGATTGTCGGTATTATCGGCAGTTTACAG
GCGTTAGAAGCGCTGAAATTTTTACTGGGTTTACCCGTTTCTTGTGACGGAAAGTTCGGTGTGTTTGACGGAAGAAGTTT
GATGTGGCAACAAATTGAAATGGTAAAAGATAAACGTTGCCGAGTTTGCGGAACATTATAA

Upstream 100 bases:

>100_bases
GCTAAATTTATAAGCGATTAATCCGTCGGTTGAAGTTGTCGCTATCAATAAACAATTAACTGCCGACAATTTGACGTATT
GGGTAAATAAAGTCAATGTA

Downstream 100 bases:

>100_bases
GTTATAAACGAATTTTGAAAGTGAGGAAATATGCAAATTTTCATTAATAATCAACCGCTTGACGTAGCGGAAAATACGTC
AATTCAAACGGCGTTAGCAC

Product: molybdopterin/thiamine biosynthesis dinucleotide-utilizing protein

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 126

Protein sequence:

>126_residues
MLDCSDNMATRHAVNKVCVENQIPLISGSAVGMDGQLLGVFPPYQHGCYACLFSDTEIGNLNCRTAGVLAPIVGIIGSLQ
ALEALKFLLGLPVSCDGKFGVFDGRSLMWQQIEMVKDKRCRVCGTL

Sequences:

>Translated_126_residues
MLDCSDNMATRHAVNKVCVENQIPLISGSAVGMDGQLLGVFPPYQHGCYACLFSDTEIGNLNCRTAGVLAPIVGIIGSLQ
ALEALKFLLGLPVSCDGKFGVFDGRSLMWQQIEMVKDKRCRVCGTL
>Mature_126_residues
MLDCSDNMATRHAVNKVCVENQIPLISGSAVGMDGQLLGVFPPYQHGCYACLFSDTEIGNLNCRTAGVLAPIVGIIGSLQ
ALEALKFLLGLPVSCDGKFGVFDGRSLMWQQIEMVKDKRCRVCGTL

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Escherichia coli, GI87082356, Length=124, Percent_Identity=48.3870967741936, Blast_Score=112, Evalue=6e-27,
Organism=Escherichia coli, GI1787048, Length=126, Percent_Identity=41.2698412698413, Blast_Score=100, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17540406, Length=129, Percent_Identity=45.7364341085271, Blast_Score=100, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24582879, Length=129, Percent_Identity=38.7596899224806, Blast_Score=82, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012731
- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 13552; Mature: 13552

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
10.3 %Cys+Met (Translated Protein)
6.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
10.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDCSDNMATRHAVNKVCVENQIPLISGSAVGMDGQLLGVFPPYQHGCYACLFSDTEIGN
CCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEEECCCCCCCEEEEEECCCCCCC
LNCRTAGVLAPIVGIIGSLQALEALKFLLGLPVSCDGKFGVFDGRSLMWQQIEMVKDKRC
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
RVCGTL
CCCCCC
>Mature Secondary Structure
MLDCSDNMATRHAVNKVCVENQIPLISGSAVGMDGQLLGVFPPYQHGCYACLFSDTEIGN
CCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEEECCCCCCCEEEEEECCCCCCC
LNCRTAGVLAPIVGIIGSLQALEALKFLLGLPVSCDGKFGVFDGRSLMWQQIEMVKDKRC
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
RVCGTL
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 9632726; 10082377 [H]