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The map label for this gene is thiF [H]

Identifier: 126208014

GI number: 126208014

Start: 605200

End: 605481

Strand: Direct

Name: thiF [H]

Synonym: APL_0532

Alternate gene names: 126208014

Gene position: 605200-605481 (Clockwise)

Preceding gene: 126208013

Following gene: 126208015

Centisome position: 26.61

GC content: 45.39

Gene sequence:

>282_bases
ATGAGTTTATCTGATCAAGATTTCCTGCGTTATAGCCGCCAAATTCTGTTGGAGGAATGCGGCATTGAAGGGCAGCAAAA
GTTAGCCAAAAGTCGAGTATTGGTAGTGGGCTGCGGCGGACTCGGTTCGCCGGTTGCACAATATCTCGCATCCGCCGGTG
TCGGAAAACTTTATTTAGCCGACTTTGATAAGGTGGATTTAACCAATTTACAACGCCAAACATTGTTCGGCATGAAAGAT
TTGAATAAGCCGAAAAGCGAGCAGGCTCGGCTAAATTTATAA

Upstream 100 bases:

>100_bases
TTTATTACCGACAGGCTACTACGCCGTTGGCTGCCGTGGGTTCAATATATGCGATAGTGCAAGCGGTTGATTTTCTAAGA
TTTTTTACTAAGAGATTGTT

Downstream 100 bases:

>100_bases
GCGATTAATCCGTCGGTTGAAGTTGTCGCTATCAATAAACAATTAACTGCCGACAATTTGACGTATTGGGTAAATAAAGT
CAATGTAGTGTTGGATTGCT

Product: molybdopterin/thiamine biosynthesis dinucleotide-utilizing protein

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 93; Mature: 92

Protein sequence:

>93_residues
MSLSDQDFLRYSRQILLEECGIEGQQKLAKSRVLVVGCGGLGSPVAQYLASAGVGKLYLADFDKVDLTNLQRQTLFGMKD
LNKPKSEQARLNL

Sequences:

>Translated_93_residues
MSLSDQDFLRYSRQILLEECGIEGQQKLAKSRVLVVGCGGLGSPVAQYLASAGVGKLYLADFDKVDLTNLQRQTLFGMKD
LNKPKSEQARLNL
>Mature_92_residues
SLSDQDFLRYSRQILLEECGIEGQQKLAKSRVLVVGCGGLGSPVAQYLASAGVGKLYLADFDKVDLTNLQRQTLFGMKDL
NKPKSEQARLNL

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=76, Percent_Identity=57.8947368421053, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI87082356, Length=85, Percent_Identity=60, Blast_Score=110, Evalue=2e-26,
Organism=Escherichia coli, GI1787048, Length=88, Percent_Identity=59.0909090909091, Blast_Score=101, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17540406, Length=85, Percent_Identity=43.5294117647059, Blast_Score=81, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321903, Length=89, Percent_Identity=47.1910112359551, Blast_Score=78, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6320598, Length=69, Percent_Identity=42.0289855072464, Blast_Score=62, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24582879, Length=91, Percent_Identity=49.4505494505495, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012731
- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 10248; Mature: 10117

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSDQDFLRYSRQILLEECGIEGQQKLAKSRVLVVGCGGLGSPVAQYLASAGVGKLYLA
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCEEEEECCCCCHHHHHHHHHCCCCEEEEC
DFDKVDLTNLQRQTLFGMKDLNKPKSEQARLNL
CCCCCCHHHHHHHHHHCHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SLSDQDFLRYSRQILLEECGIEGQQKLAKSRVLVVGCGGLGSPVAQYLASAGVGKLYLA
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCEEEEECCCCCHHHHHHHHHCCCCEEEEC
DFDKVDLTNLQRQTLFGMKDLNKPKSEQARLNL
CCCCCCHHHHHHHHHHCHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 9632726; 10082377 [H]