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The map label for this gene is hns [H]

Identifier: 126207932

GI number: 126207932

Start: 515572

End: 515979

Strand: Reverse

Name: hns [H]

Synonym: APL_0448

Alternate gene names: 126207932

Gene position: 515979-515572 (Counterclockwise)

Preceding gene: 126207935

Following gene: 126207931

Centisome position: 22.69

GC content: 39.95

Gene sequence:

>408_bases
ATGTCAGACATTTTAAAAACGTTATCAAATATCCGTAATCTAAGAGCGCTCGCGAAAGATTCAACCTTAGAACAAATGGA
ATCTTTACTTGAGAAAGTATCGATTGTCGTTGAAGAAAAGCGTGAATTAGTAAAAGCGCAAGAACTTGAGCAAGCGAAAG
TCATGGAAGGACTAAAGAAATATAAGGAGTTATTAGCACAGGACGGAATCTCGGCAGAAGAACTCGTAGCATTATTGAAT
AATGCCACGGAACACAAGAAACGTGAGCCTCGCGCACCTCGTCCAGCAAAATACAAATATGTTGATGAAAACGGCAATGA
AAAAACTTGGACGGGACAAGGACGTACGCCTCGCCCGATTCAAAAAGCACTGGATGAAGGTAAAAGTTTAGCATCTTTTG
CTATATAA

Upstream 100 bases:

>100_bases
AATATATAAAATATATTTTCAGGATAATTATCATTCTGAAATGCCGCAATAATTATACTATTTTCTATTATTTTATATGG
TCTTTATTTGGAGATAAAAA

Downstream 100 bases:

>100_bases
TTTTTCATCATAAAAAACGGGACTAATATAATATTAATCCCGTTTTTTTATTTTACTTAATTTATACAATATTAATCTTT
TAAATAACCAATAATTATTT

Product: DNA-binding protein H-NS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 135; Mature: 134

Protein sequence:

>135_residues
MSDILKTLSNIRNLRALAKDSTLEQMESLLEKVSIVVEEKRELVKAQELEQAKVMEGLKKYKELLAQDGISAEELVALLN
NATEHKKREPRAPRPAKYKYVDENGNEKTWTGQGRTPRPIQKALDEGKSLASFAI

Sequences:

>Translated_135_residues
MSDILKTLSNIRNLRALAKDSTLEQMESLLEKVSIVVEEKRELVKAQELEQAKVMEGLKKYKELLAQDGISAEELVALLN
NATEHKKREPRAPRPAKYKYVDENGNEKTWTGQGRTPRPIQKALDEGKSLASFAI
>Mature_134_residues
SDILKTLSNIRNLRALAKDSTLEQMESLLEKVSIVVEEKRELVKAQELEQAKVMEGLKKYKELLAQDGISAEELVALLNN
ATEHKKREPRAPRPAKYKYVDENGNEKTWTGQGRTPRPIQKALDEGKSLASFAI

Specific function: Binds tightly to ds-DNA, increases its thermal stability and inhibits transcription [H]

COG id: COG2916

COG function: function code R; DNA-binding protein H-NS

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the histone-like protein H-NS family [H]

Homologues:

Organism=Escherichia coli, GI1787489, Length=136, Percent_Identity=52.2058823529412, Blast_Score=118, Evalue=2e-28,
Organism=Escherichia coli, GI1789023, Length=137, Percent_Identity=48.1751824817518, Blast_Score=113, Evalue=6e-27,

Paralogues:

None

Copy number: 820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1780 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 1020 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 4632 Molecules/Cell In: Gr

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001801 [H]

Pfam domain/function: PF00816 Histone_HNS [H]

EC number: NA

Molecular weight: Translated: 15248; Mature: 15117

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDILKTLSNIRNLRALAKDSTLEQMESLLEKVSIVVEEKRELVKAQELEQAKVMEGLKK
CHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YKELLAQDGISAEELVALLNNATEHKKREPRAPRPAKYKYVDENGNEKTWTGQGRTPRPI
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCCHHH
QKALDEGKSLASFAI
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDILKTLSNIRNLRALAKDSTLEQMESLLEKVSIVVEEKRELVKAQELEQAKVMEGLKK
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YKELLAQDGISAEELVALLNNATEHKKREPRAPRPAKYKYVDENGNEKTWTGQGRTPRPI
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCCHHH
QKALDEGKSLASFAI
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]