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The map label for this gene is ksgA

Identifier: 126207883

GI number: 126207883

Start: 453463

End: 454332

Strand: Reverse

Name: ksgA

Synonym: APL_0399

Alternate gene names: 126207883

Gene position: 454332-453463 (Counterclockwise)

Preceding gene: 126207884

Following gene: 126207876

Centisome position: 19.98

GC content: 42.3

Gene sequence:

>870_bases
ATGAGTAATCAAAATTCAAAAAAACATTTAGGTCATACCGCCCGTAAGCGTTTCGGTCAAAACTTTTTACACGATATGAA
TGTTATTCATAATATCGTATCTGCCATCAATCCGAAAAACGGTCAATTTTTACTTGAAATCGGTCCGGGTTTAGGCGCAT
TAACCGAACCGGTCGCCGAGCAAGTGGATAAACTCACGGTGGTTGAGCTGGACAGAGATCTCGCCGAGCGTTTACGTCAT
CACCCGTTCTTAAATCATAAATTAACCATTATCGAACAAGATGCGTTACGTTTTAATTTCCGTGAATATTTTGAAAGTCT
CGAGCTAAGAGAAGGTGAAGGGGTTCGTGTATTCGGTAACTTGCCGTATAACATCTCAACGCCGTTGATGTTCCACTTAT
TCAAATTCCACGATTTGATTCAAGATATGCATTTTATGTTGCAAAAAGAGGTGGTAAAACGTCTATGTGCCGCACCGAAT
AGTAAAGCGTACGGTCGTTTAACTATTATGGCGCAATATTATTGTCAAGTAATGCCTGTGTTAGAAGTGCCGCCGACCGC
ATTTAAACCGGCACCTAAGGTAGATTCCGCCGTGGTACGTTTAATGCCGCATAAAGTATTACCGCATCCGGTAAAAGACG
TATATTGGTTAAACCGTGTAACTACACAAGCCTTCAACCAGCGTCGTAAAACCTTGCGTAACGCACTCTCAACGCTATTT
AGCCCCGAGCAATTAGAAGCGTTGAATATCGACCTAAATGCACGTGCGGAAAACTTAAGTATTGCGGACTATGCCCGTTT
AGCCAACTGGTTATATGACAATCCGCCGGCGGTGGATAACCAAGAAGAAATCATTGACGAAGATATCTAA

Upstream 100 bases:

>100_bases
GAAAAACCGCTGACGTTCAGTACGTTGGTAAATAAACATAACGATTTATCTGAGAACCTCGCTATTTGCGGGGTTCTTTG
CTTATTTTAAAAGGATAACA

Downstream 100 bases:

>100_bases
TCTGACAAGCGGTTAAATTTGTAAAAAATTTTGCAAATTCCACCGCTTAGTCATAGATATAAAAAAACCTAAGAGATTCT
CTTAGGTTTTTTGTTTTTAT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MSNQNSKKHLGHTARKRFGQNFLHDMNVIHNIVSAINPKNGQFLLEIGPGLGALTEPVAEQVDKLTVVELDRDLAERLRH
HPFLNHKLTIIEQDALRFNFREYFESLELREGEGVRVFGNLPYNISTPLMFHLFKFHDLIQDMHFMLQKEVVKRLCAAPN
SKAYGRLTIMAQYYCQVMPVLEVPPTAFKPAPKVDSAVVRLMPHKVLPHPVKDVYWLNRVTTQAFNQRRKTLRNALSTLF
SPEQLEALNIDLNARAENLSIADYARLANWLYDNPPAVDNQEEIIDEDI

Sequences:

>Translated_289_residues
MSNQNSKKHLGHTARKRFGQNFLHDMNVIHNIVSAINPKNGQFLLEIGPGLGALTEPVAEQVDKLTVVELDRDLAERLRH
HPFLNHKLTIIEQDALRFNFREYFESLELREGEGVRVFGNLPYNISTPLMFHLFKFHDLIQDMHFMLQKEVVKRLCAAPN
SKAYGRLTIMAQYYCQVMPVLEVPPTAFKPAPKVDSAVVRLMPHKVLPHPVKDVYWLNRVTTQAFNQRRKTLRNALSTLF
SPEQLEALNIDLNARAENLSIADYARLANWLYDNPPAVDNQEEIIDEDI
>Mature_288_residues
SNQNSKKHLGHTARKRFGQNFLHDMNVIHNIVSAINPKNGQFLLEIGPGLGALTEPVAEQVDKLTVVELDRDLAERLRHH
PFLNHKLTIIEQDALRFNFREYFESLELREGEGVRVFGNLPYNISTPLMFHLFKFHDLIQDMHFMLQKEVVKRLCAAPNS
KAYGRLTIMAQYYCQVMPVLEVPPTAFKPAPKVDSAVVRLMPHKVLPHPVKDVYWLNRVTTQAFNQRRKTLRNALSTLFS
PEQLEALNIDLNARAENLSIADYARLANWLYDNPPAVDNQEEIIDEDI

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=284, Percent_Identity=31.6901408450704, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI7657198, Length=257, Percent_Identity=29.9610894941634, Blast_Score=97, Evalue=1e-20,
Organism=Escherichia coli, GI1786236, Length=272, Percent_Identity=67.2794117647059, Blast_Score=384, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI25141369, Length=285, Percent_Identity=29.8245614035088, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI25146882, Length=218, Percent_Identity=34.4036697247706, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324989, Length=203, Percent_Identity=31.0344827586207, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21357273, Length=281, Percent_Identity=30.9608540925267, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21358017, Length=246, Percent_Identity=32.9268292682927, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_ACTP2 (A3MZB7)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053108.1
- ProteinModelPortal:   A3MZB7
- SMR:   A3MZB7
- STRING:   A3MZB7
- GeneID:   4850436
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0399
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   RAENLTP
- PhylomeDB:   A3MZB7
- ProtClustDB:   PRK00274
- BioCyc:   APLE416269:APL_0399-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 33273; Mature: 33142

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 21-21 BINDING 23-23 BINDING 48-48 BINDING 69-69 BINDING 94-94 BINDING 120-120

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQNSKKHLGHTARKRFGQNFLHDMNVIHNIVSAINPKNGQFLLEIGPGLGALTEPVAE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHH
QVDKLTVVELDRDLAERLRHHPFLNHKLTIIEQDALRFNFREYFESLELREGEGVRVFGN
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEC
LPYNISTPLMFHLFKFHDLIQDMHFMLQKEVVKRLCAAPNSKAYGRLTIMAQYYCQVMPV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
LEVPPTAFKPAPKVDSAVVRLMPHKVLPHPVKDVYWLNRVTTQAFNQRRKTLRNALSTLF
HCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SPEQLEALNIDLNARAENLSIADYARLANWLYDNPPAVDNQEEIIDEDI
CHHHHHEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCC
>Mature Secondary Structure 
SNQNSKKHLGHTARKRFGQNFLHDMNVIHNIVSAINPKNGQFLLEIGPGLGALTEPVAE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHH
QVDKLTVVELDRDLAERLRHHPFLNHKLTIIEQDALRFNFREYFESLELREGEGVRVFGN
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEC
LPYNISTPLMFHLFKFHDLIQDMHFMLQKEVVKRLCAAPNSKAYGRLTIMAQYYCQVMPV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
LEVPPTAFKPAPKVDSAVVRLMPHKVLPHPVKDVYWLNRVTTQAFNQRRKTLRNALSTLF
HCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SPEQLEALNIDLNARAENLSIADYARLANWLYDNPPAVDNQEEIIDEDI
CHHHHHEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA