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The map label for this gene is rluE [H]

Identifier: 126207576

GI number: 126207576

Start: 95649

End: 96344

Strand: Reverse

Name: rluE [H]

Synonym: APL_0088

Alternate gene names: 126207576

Gene position: 96344-95649 (Counterclockwise)

Preceding gene: 126207577

Following gene: 126207575

Centisome position: 4.24

GC content: 40.95

Gene sequence:

>696_bases
ATGTTTAAAGCTAAATTTAATCAAAAAAATAAAGCTCGTTCCGCAACAAACAAACCGAACTTGCAAAATAGAACCAAAAA
ACGACCGCTTAAACCACAGCTTTCGTTTGAAGATACTGTGGTTGTATTGTTTAACAAGCCGTTTGACGTGCTAACCCAAT
TCACTGATGAACAAGGGCGACAAACGCTGAAAGATTTTATTCCGATTCCACAGATTTATCCGGTCGGCCGTTTAGATCGT
GACAGCGAAGGCTTATTGTTACTGACTAATAACGGTGAAGTTCAGCACCGCCTAGCAAATCCTAAATTTGAAAAGCAAAA
AACTTATCTCGCACAGGTGGAAGGTATTCCGCAAGCTGCGGATTTAGCTAAGTTAGAGAAAGGTGTTGAATTGAAAGACG
GCTTAACCAAGCCGGCAAAAGCGAAAGCGATTTCCGCCCCGAATTATGAATGGCAAACCGCCCCAAAAATTCGAGAACGA
AAAACCATTCCAACTAGTTGGATTGAGCTAAAAATCCACGAAGGGAAAAACCGCCAAGTACGCCGAATGACCGCACACAT
CGGCTTTCCGACCCTACGCTTAATCCGTATCGGTTTAGGACAATTTCAGCTAGGCGAGTTAAATAGCGGTGAATATCGAG
TTTTAGATGAAAAAGAAAAACGCCAATTATTTCAACAAATTGACTTAAAAATTTAA

Upstream 100 bases:

>100_bases
TGCGTGGCTATGTTTTAACGAAGTACCGACATTAACAATGTTGATTGGCGGTGCGATTATTTTACTCTCGGTCGCTTGGG
CTAACTTAAAGAAATAAATG

Downstream 100 bases:

>100_bases
TGATTTCGGGAGCAAATATGAAAAGTTATTGGGTATTAGTACATCATTTTGATCTTGCTTTAAAAGATGATGAAATTCCT
TTCGGTACAGCCGAACAATT

Product: ribosomal large subunit pseudouridine synthase E

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MFKAKFNQKNKARSATNKPNLQNRTKKRPLKPQLSFEDTVVVLFNKPFDVLTQFTDEQGRQTLKDFIPIPQIYPVGRLDR
DSEGLLLLTNNGEVQHRLANPKFEKQKTYLAQVEGIPQAADLAKLEKGVELKDGLTKPAKAKAISAPNYEWQTAPKIRER
KTIPTSWIELKIHEGKNRQVRRMTAHIGFPTLRLIRIGLGQFQLGELNSGEYRVLDEKEKRQLFQQIDLKI

Sequences:

>Translated_231_residues
MFKAKFNQKNKARSATNKPNLQNRTKKRPLKPQLSFEDTVVVLFNKPFDVLTQFTDEQGRQTLKDFIPIPQIYPVGRLDR
DSEGLLLLTNNGEVQHRLANPKFEKQKTYLAQVEGIPQAADLAKLEKGVELKDGLTKPAKAKAISAPNYEWQTAPKIRER
KTIPTSWIELKIHEGKNRQVRRMTAHIGFPTLRLIRIGLGQFQLGELNSGEYRVLDEKEKRQLFQQIDLKI
>Mature_231_residues
MFKAKFNQKNKARSATNKPNLQNRTKKRPLKPQLSFEDTVVVLFNKPFDVLTQFTDEQGRQTLKDFIPIPQIYPVGRLDR
DSEGLLLLTNNGEVQHRLANPKFEKQKTYLAQVEGIPQAADLAKLEKGVELKDGLTKPAKAKAISAPNYEWQTAPKIRER
KTIPTSWIELKIHEGKNRQVRRMTAHIGFPTLRLIRIGLGQFQLGELNSGEYRVLDEKEKRQLFQQIDLKI

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=175, Percent_Identity=58.8571428571429, Blast_Score=218, Evalue=3e-58,
Organism=Escherichia coli, GI1788510, Length=184, Percent_Identity=34.7826086956522, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1787524, Length=216, Percent_Identity=31.9444444444444, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1790453, Length=193, Percent_Identity=26.9430051813472, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 26612; Mature: 26612

Theoretical pI: Translated: 10.70; Mature: 10.70

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKAKFNQKNKARSATNKPNLQNRTKKRPLKPQLSFEDTVVVLFNKPFDVLTQFTDEQGR
CCCCCCCCCHHHHCCCCCCCCCHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHH
QTLKDFIPIPQIYPVGRLDRDSEGLLLLTNNGEVQHRLANPKFEKQKTYLAQVEGIPQAA
HHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHCCCCCHH
DLAKLEKGVELKDGLTKPAKAKAISAPNYEWQTAPKIRERKTIPTSWIELKIHEGKNRQV
HHHHHHCCCCHHHCCCCCCCCCEECCCCCCCCCCCCHHHCCCCCCEEEEEEEECCCCCHH
RRMTAHIGFPTLRLIRIGLGQFQLGELNSGEYRVLDEKEKRQLFQQIDLKI
HHHHHHCCCCHHHHHHHCCCCEEECCCCCCCEEEECCHHHHHHHHHHCCCC
>Mature Secondary Structure
MFKAKFNQKNKARSATNKPNLQNRTKKRPLKPQLSFEDTVVVLFNKPFDVLTQFTDEQGR
CCCCCCCCCHHHHCCCCCCCCCHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHH
QTLKDFIPIPQIYPVGRLDRDSEGLLLLTNNGEVQHRLANPKFEKQKTYLAQVEGIPQAA
HHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHCCCCCHH
DLAKLEKGVELKDGLTKPAKAKAISAPNYEWQTAPKIRERKTIPTSWIELKIHEGKNRQV
HHHHHHCCCCHHHCCCCCCCCCEECCCCCCCCCCCCHHHCCCCCCEEEEEEEECCCCCHH
RRMTAHIGFPTLRLIRIGLGQFQLGELNSGEYRVLDEKEKRQLFQQIDLKI
HHHHHHCCCCHHHHHHHCCCCEEECCCCCCCEEEECCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]