Click here to switch to the map view.

The map label for this gene is dhaK [H]

Identifier: 126207571

GI number: 126207571

Start: 91185

End: 92255

Strand: Reverse

Name: dhaK [H]

Synonym: APL_0083

Alternate gene names: 126207571

Gene position: 92255-91185 (Counterclockwise)

Preceding gene: 126207575

Following gene: 126207570

Centisome position: 4.06

GC content: 45.19

Gene sequence:

>1071_bases
ATGAAAAAACTTATCAATTCAATCGAAACTGTCTTACAGGAACAGTTAACCGGTTTTGCCAAAGCACATCCTACTATTAC
TCTGAATACCGAGCCGACTTATGTACGCCGAGCTGACGCACCGGTTGCCGGTAAAGTGGCATTGATTTCCGGCGGCGGGA
GCGGACACGAGCCGATGCACGCCGGTTTTGTGGGTAAGGGAATGTTAGACGGTGCTTGTCCGGGTGCGATTTTTACTTCT
CCGACACCGGATCAAATGTTTGAATGCGGTTTAAATGTCGATAGCGGCGAAGGCGTGTTATTACTTATTAAAAATTATAC
CGGTGACGTTTTAAATTTTGAAACCGCAACCGAACTTCTTGCCGATAGTGGGGTAAAAGTCGCAACCGTATTAATTGATG
ATGACGTAGCGGTCAAAGACAGCTTATATACCGCCGGTCGCCGCGGCGTTGCTAACACAGTGTTATTAGAAAAACTATTA
GGCGCAGCGGCAGATAAAGGTTACAACTTATCGCAATTAGCCGAACTCGGTTACAAATTAAATAATGCAGGTCATTCGAT
TGGTGTTGCGCTTGGCGCTTGTACCGTACCTGCAGCCGGTAAACCGTCTTTCACTTTAGCCGAAAACGAAATGGAATTTG
GTGTAGGGATTCACGGCGAACCGGGTATTGAACGCCGTCCGTTTGAGAACCTCGATAAAACCGTACGCCAAATGTTTGAA
ACACTCATTGCACACGGCGATTATGAGCGTACTGTACGCCGTTGGGATAACGACACTCAACAATGGAACGAAGTGTTAGA
TAAAAAACAAGCGTTACAAAAAGGCGATCGTGTGATTGCGTTAGTGAATAACTTAGGTGCGGTACCGCTTTCAGAATTAT
ATGGTGTTTACAATGTGCTTGCCGATTGTTGTGAACAATTCGGTTTAACGATTGAGCGTAATTTGGTCGGTTCATTCTGT
ACCTCACTCGATATGCAAGGTGTTTCTATTACCTTACTAAAAGTCGATGATGAAACCTTAGCACTTTGGGATGCACCGGT
TAATACGCCGGCATTACGTTGGGGAGAATAA

Upstream 100 bases:

>100_bases
TCACTCATCTTTTTTTCGATTTTGTGAAGTAACTAACAGAAATCTGCCTCGAATTTTGGTTAAATTGGGACAAATTCTTC
TTTTAAAAGGATAAACACCG

Downstream 100 bases:

>100_bases
GATGGCGATTTCAAAACAACAAATCCTACAATGGCTTGAAAATTGTAATCAGGTGATGACTGAGCAACGAGATTTTCTCA
CACAGTTGGATACCGAAATC

Product: dihydroxyacetone kinase subunit DhaK

Products: ADP; glycerone phosphate

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MKKLINSIETVLQEQLTGFAKAHPTITLNTEPTYVRRADAPVAGKVALISGGGSGHEPMHAGFVGKGMLDGACPGAIFTS
PTPDQMFECGLNVDSGEGVLLLIKNYTGDVLNFETATELLADSGVKVATVLIDDDVAVKDSLYTAGRRGVANTVLLEKLL
GAAADKGYNLSQLAELGYKLNNAGHSIGVALGACTVPAAGKPSFTLAENEMEFGVGIHGEPGIERRPFENLDKTVRQMFE
TLIAHGDYERTVRRWDNDTQQWNEVLDKKQALQKGDRVIALVNNLGAVPLSELYGVYNVLADCCEQFGLTIERNLVGSFC
TSLDMQGVSITLLKVDDETLALWDAPVNTPALRWGE

Sequences:

>Translated_356_residues
MKKLINSIETVLQEQLTGFAKAHPTITLNTEPTYVRRADAPVAGKVALISGGGSGHEPMHAGFVGKGMLDGACPGAIFTS
PTPDQMFECGLNVDSGEGVLLLIKNYTGDVLNFETATELLADSGVKVATVLIDDDVAVKDSLYTAGRRGVANTVLLEKLL
GAAADKGYNLSQLAELGYKLNNAGHSIGVALGACTVPAAGKPSFTLAENEMEFGVGIHGEPGIERRPFENLDKTVRQMFE
TLIAHGDYERTVRRWDNDTQQWNEVLDKKQALQKGDRVIALVNNLGAVPLSELYGVYNVLADCCEQFGLTIERNLVGSFC
TSLDMQGVSITLLKVDDETLALWDAPVNTPALRWGE
>Mature_356_residues
MKKLINSIETVLQEQLTGFAKAHPTITLNTEPTYVRRADAPVAGKVALISGGGSGHEPMHAGFVGKGMLDGACPGAIFTS
PTPDQMFECGLNVDSGEGVLLLIKNYTGDVLNFETATELLADSGVKVATVLIDDDVAVKDSLYTAGRRGVANTVLLEKLL
GAAADKGYNLSQLAELGYKLNNAGHSIGVALGACTVPAAGKPSFTLAENEMEFGVGIHGEPGIERRPFENLDKTVRQMFE
TLIAHGDYERTVRRWDNDTQQWNEVLDKKQALQKGDRVIALVNNLGAVPLSELYGVYNVLADCCEQFGLTIERNLVGSFC
TSLDMQGVSITLLKVDDETLALWDAPVNTPALRWGE

Specific function: Dihydroxyacetone binding subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone. Binds covalently dihydroxyacetone in hemiaminal linkage. Acts also as a corepressor of dhaR by binding to its sensor domain, in the

COG id: COG2376

COG function: function code G; Dihydroxyacetone kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DhaK domain [H]

Homologues:

Organism=Homo sapiens, GI20149621, Length=356, Percent_Identity=38.7640449438202, Blast_Score=218, Evalue=5e-57,
Organism=Escherichia coli, GI87081857, Length=356, Percent_Identity=75.8426966292135, Blast_Score=568, Evalue=1e-163,
Organism=Caenorhabditis elegans, GI17565018, Length=351, Percent_Identity=34.1880341880342, Blast_Score=186, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6321055, Length=364, Percent_Identity=35.1648351648352, Blast_Score=196, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6323570, Length=340, Percent_Identity=37.6470588235294, Blast_Score=184, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004006
- InterPro:   IPR012736 [H]

Pfam domain/function: PF02733 Dak1 [H]

EC number: 2.7.1.29

Molecular weight: Translated: 38225; Mature: 38225

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLINSIETVLQEQLTGFAKAHPTITLNTEPTYVRRADAPVAGKVALISGGGSGHEPMH
CHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEECCCCCCCEEEEEECCCCCCCCCC
AGFVGKGMLDGACPGAIFTSPTPDQMFECGLNVDSGEGVLLLIKNYTGDVLNFETATELL
CCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEEEEECCCCCEECHHHHHHHH
ADSGVKVATVLIDDDVAVKDSLYTAGRRGVANTVLLEKLLGAAADKGYNLSQLAELGYKL
HCCCCEEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
NNAGHSIGVALGACTVPAAGKPSFTLAENEMEFGVGIHGEPGIERRPFENLDKTVRQMFE
CCCCCCCEEEEHHEECCCCCCCCEEEECCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
TLIAHGDYERTVRRWDNDTQQWNEVLDKKQALQKGDRVIALVNNLGAVPLSELYGVYNVL
HHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHH
ADCCEQFGLTIERNLVGSFCTSLDMQGVSITLLKVDDETLALWDAPVNTPALRWGE
HHHHHHHCCEEEHHHHHHHHHHCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MKKLINSIETVLQEQLTGFAKAHPTITLNTEPTYVRRADAPVAGKVALISGGGSGHEPMH
CHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEECCCCCCCEEEEEECCCCCCCCCC
AGFVGKGMLDGACPGAIFTSPTPDQMFECGLNVDSGEGVLLLIKNYTGDVLNFETATELL
CCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEEEEECCCCCEECHHHHHHHH
ADSGVKVATVLIDDDVAVKDSLYTAGRRGVANTVLLEKLLGAAADKGYNLSQLAELGYKL
HCCCCEEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
NNAGHSIGVALGACTVPAAGKPSFTLAENEMEFGVGIHGEPGIERRPFENLDKTVRQMFE
CCCCCCCEEEEHHEECCCCCCCCEEEECCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
TLIAHGDYERTVRRWDNDTQQWNEVLDKKQALQKGDRVIALVNNLGAVPLSELYGVYNVL
HHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHH
ADCCEQFGLTIERNLVGSFCTSLDMQGVSITLLKVDDETLALWDAPVNTPALRWGE
HHHHHHHCCEEEHHHHHHHHHHCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; glycerone

Specific reaction: ATP + glycerone = ADP + glycerone phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]