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The map label for this gene is ilvE [H]

Identifier: 126207560

GI number: 126207560

Start: 84149

End: 85186

Strand: Reverse

Name: ilvE [H]

Synonym: APL_0072

Alternate gene names: 126207560

Gene position: 85186-84149 (Counterclockwise)

Preceding gene: 126207561

Following gene: 126207559

Centisome position: 3.75

GC content: 46.24

Gene sequence:

>1038_bases
ATGGCTTTAAAAGATTTAGACTGGGCCAATTTAGGCTTCTCATATATCAAAACGGATTATCGCTTCATCGCCCACTGGAA
AGACGGTAAATGGGACGAAGGTCAATTAACCACGGATAACACTCTCCATATTCACGAAGGTTCCACCGCACTTCACTACG
GTCAACAATGTTTCGAAGGCTTAAAAGCTTATCGCTGCAAAGACGGTTCTATTAACTTATTCCGCCCTGAAGAGAATGCA
AAACGTATGCAAGGCACTGCTCGCCGTTTATTAATGGCGGAAGTGCCGACCGAATTATTTGTACGCGCCTGTAAAGAAGT
GGTGAAAGCAAACCAAGATTGGTTAGGCCCGTACGGCTCGGGTGCAACGCTTTACTTACGTCCGTTCTTAATCGGCGTGG
GTGATAACATCGGAGTGAAAGCGGCGCCGGAATACATTTTCTCGGTATTCTGCTGTCCGGTCGGTGCTTATTTCAAAGGC
GGTTTAGCACCGTCCAACTTCATCACAACCGACTATGACCGTGCCGCACCGGTTGGAACCGGCGGAGTTAAAGTCGGCGG
TAACTATGCGGCAAGTTTGTTACCGCACGAATTAGCCGTACAACAAGGTACGGCAACCCGTAAATTTGCCGATGCGATCT
ACCTTGATCCGAAAACTCACACCAAAATTGAAGAAGTGGGTGCGGCAAACTTCTTCGGGATTACCAAAGACAATAAATTT
ATTACTCCTGCGTCAGAATCAATCTTACCAAGTATTACCAAATACTCGTTATTACATATTGCTAAAGAACGTTTAGGTAT
GGAAACGATTGAGGGTGACGTATATATCGATCAATTGGATCAATTTGCTGAAGCCGGTGCTTGCGGTACGGCGGCGGTAA
TTACACCGGTCGGCGGTATTCAACATAAAGATAACTTCCACGTATTCTATTCGGAAACGGAAGTCGGCCCGGTAACAAAA
CGCCTATATGCGGAACTTACCGGTATCCAATTCGGTGATGTCGAAGCGCCGGAAGGCTGGATTGTTAAAGTAGAATAA

Upstream 100 bases:

>100_bases
TTTTATCGAAAAAATGCCGTTTTGCTCTTACATTCTTATTCGTTTTTGGCTTACAATGTAAAAATTCATTTGTAATACAT
AACAATAAGGACACAACAAC

Downstream 100 bases:

>100_bases
GCCGATTTAAGCTATGCAAGCGGTAAAATTTTGCAATTTTTTTGCAAATTTCTACCGCTTGTTTTCTTTCAAACATAAGG
AATATTATGATTCAACGTAT

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MALKDLDWANLGFSYIKTDYRFIAHWKDGKWDEGQLTTDNTLHIHEGSTALHYGQQCFEGLKAYRCKDGSINLFRPEENA
KRMQGTARRLLMAEVPTELFVRACKEVVKANQDWLGPYGSGATLYLRPFLIGVGDNIGVKAAPEYIFSVFCCPVGAYFKG
GLAPSNFITTDYDRAAPVGTGGVKVGGNYAASLLPHELAVQQGTATRKFADAIYLDPKTHTKIEEVGAANFFGITKDNKF
ITPASESILPSITKYSLLHIAKERLGMETIEGDVYIDQLDQFAEAGACGTAAVITPVGGIQHKDNFHVFYSETEVGPVTK
RLYAELTGIQFGDVEAPEGWIVKVE

Sequences:

>Translated_345_residues
MALKDLDWANLGFSYIKTDYRFIAHWKDGKWDEGQLTTDNTLHIHEGSTALHYGQQCFEGLKAYRCKDGSINLFRPEENA
KRMQGTARRLLMAEVPTELFVRACKEVVKANQDWLGPYGSGATLYLRPFLIGVGDNIGVKAAPEYIFSVFCCPVGAYFKG
GLAPSNFITTDYDRAAPVGTGGVKVGGNYAASLLPHELAVQQGTATRKFADAIYLDPKTHTKIEEVGAANFFGITKDNKF
ITPASESILPSITKYSLLHIAKERLGMETIEGDVYIDQLDQFAEAGACGTAAVITPVGGIQHKDNFHVFYSETEVGPVTK
RLYAELTGIQFGDVEAPEGWIVKVE
>Mature_344_residues
ALKDLDWANLGFSYIKTDYRFIAHWKDGKWDEGQLTTDNTLHIHEGSTALHYGQQCFEGLKAYRCKDGSINLFRPEENAK
RMQGTARRLLMAEVPTELFVRACKEVVKANQDWLGPYGSGATLYLRPFLIGVGDNIGVKAAPEYIFSVFCCPVGAYFKGG
LAPSNFITTDYDRAAPVGTGGVKVGGNYAASLLPHELAVQQGTATRKFADAIYLDPKTHTKIEEVGAANFFGITKDNKFI
TPASESILPSITKYSLLHIAKERLGMETIEGDVYIDQLDQFAEAGACGTAAVITPVGGIQHKDNFHVFYSETEVGPVTKR
LYAELTGIQFGDVEAPEGWIVKVE

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI50658084, Length=337, Percent_Identity=32.3442136498516, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI38176287, Length=346, Percent_Identity=33.5260115606936, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI296010904, Length=346, Percent_Identity=33.5260115606936, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI296010906, Length=346, Percent_Identity=33.5260115606936, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI258614015, Length=292, Percent_Identity=32.8767123287671, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI296010902, Length=343, Percent_Identity=28.8629737609329, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI296010900, Length=346, Percent_Identity=28.9017341040462, Blast_Score=115, Evalue=8e-26,
Organism=Escherichia coli, GI48994963, Length=325, Percent_Identity=31.6923076923077, Blast_Score=114, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17568601, Length=364, Percent_Identity=30.2197802197802, Blast_Score=161, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17565728, Length=352, Percent_Identity=28.9772727272727, Blast_Score=114, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6322608, Length=347, Percent_Identity=32.2766570605187, Blast_Score=162, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6322002, Length=345, Percent_Identity=30.4347826086957, Blast_Score=143, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24641779, Length=350, Percent_Identity=30, Blast_Score=156, Evalue=2e-38,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 37893; Mature: 37762

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALKDLDWANLGFSYIKTDYRFIAHWKDGKWDEGQLTTDNTLHIHEGSTALHYGQQCFEG
CCCCCCCHHHCCHHHHHCCEEEEEEECCCCCCCCEECCCCEEEEECCCHHHHHHHHHHHH
LKAYRCKDGSINLFRPEENAKRMQGTARRLLMAEVPTELFVRACKEVVKANQDWLGPYGS
HHHEECCCCCEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCC
GATLYLRPFLIGVGDNIGVKAAPEYIFSVFCCPVGAYFKGGLAPSNFITTDYDRAAPVGT
CCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHCCHHHHCCCCCCCCCEECCCCCCCCCCC
GGVKVGGNYAASLLPHELAVQQGTATRKFADAIYLDPKTHTKIEEVGAANFFGITKDNKF
CCEEECCCHHHHHCHHHHHHHCCCCHHHHHHEEEECCCCCCHHHHCCCCCEEEECCCCCE
ITPASESILPSITKYSLLHIAKERLGMETIEGDVYIDQLDQFAEAGACGTAAVITPVGGI
ECCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCEEHHHHHHHHHCCCCCCEEEEECCCCC
QHKDNFHVFYSETEVGPVTKRLYAELTGIQFGDVEAPEGWIVKVE
CCCCCEEEEEECCCCCHHHHHHHHHHHCCEECCCCCCCCCEEEEC
>Mature Secondary Structure 
ALKDLDWANLGFSYIKTDYRFIAHWKDGKWDEGQLTTDNTLHIHEGSTALHYGQQCFEG
CCCCCCHHHCCHHHHHCCEEEEEEECCCCCCCCEECCCCEEEEECCCHHHHHHHHHHHH
LKAYRCKDGSINLFRPEENAKRMQGTARRLLMAEVPTELFVRACKEVVKANQDWLGPYGS
HHHEECCCCCEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCC
GATLYLRPFLIGVGDNIGVKAAPEYIFSVFCCPVGAYFKGGLAPSNFITTDYDRAAPVGT
CCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHCCHHHHCCCCCCCCCEECCCCCCCCCCC
GGVKVGGNYAASLLPHELAVQQGTATRKFADAIYLDPKTHTKIEEVGAANFFGITKDNKF
CCEEECCCHHHHHCHHHHHHHCCCCHHHHHHEEEECCCCCCHHHHCCCCCEEEECCCCCE
ITPASESILPSITKYSLLHIAKERLGMETIEGDVYIDQLDQFAEAGACGTAAVITPVGGI
ECCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCEEHHHHHHHHHCCCCCCEEEEECCCCC
QHKDNFHVFYSETEVGPVTKRLYAELTGIQFGDVEAPEGWIVKVE
CCCCCEEEEEECCCCCHHHHHHHHHHHCCEECCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]