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The map label for this gene is rlmB [H]

Identifier: 126207530

GI number: 126207530

Start: 46186

End: 46929

Strand: Reverse

Name: rlmB [H]

Synonym: APL_0042

Alternate gene names: 126207530

Gene position: 46929-46186 (Counterclockwise)

Preceding gene: 126207531

Following gene: 126207526

Centisome position: 2.06

GC content: 46.1

Gene sequence:

>744_bases
ATGAGCGAACAAATTTATGGTATTCATGCCGTAAAAGCTTTCTTAGATAACGCACCGGAACGCTTAATTGAAGTTTTAGT
GCTAAAAGGGCGTGAAGATAAACGCTTAACCCCGTTACTAAATGAGTTACAACGTTTAGGTATTTCCGTTCAGCAAGTAA
ACCGCCAAACATTGGATAACAAAGCGCAAGGTGAAGTTCACCAAGGGATTATTGCGAAAGTCGTGCCGCAAAAAGAGCTG
AACGAACATGATCTGGATGCTATTCTGGCGCAAAAACAAAACCCGTTTTTATTAATCTTAGACGGTGTGACCGATCCGCA
TAACCTTGGTGCTTGTTTGCGTACTGCAGATGCGGCAGGTGTGGATGCGGTGATTGTACCGAAAGATAAATCGGCACAGC
TTACCTCAACCGCTCGTAAAGTAGCTTGCGGTGCGGCGGAAACCGTGCCGTTAATTCGTGTTACCAACCTTGCTCGCACA
ATGCGAGAGCTACAAGAGCAACATAATATCTGGATTGTCGGCACGGCAGGTGAAGCGACTTCCGGTATTTATCAGGCTAA
ATTAACCGGTGCTATCGCTTTAGTGATGGGAGCGGAAGGTGACGGTATGCGTCGTCTAACCCGTGAACATTGCGACCAGT
TAATCAGTATCCCAATGGCGGGTTCGGTTTCTTCATTGAACGTTTCGGTTGCGACCGGCGTATGTTTATTTGAAATCGTA
CGCCAAAAACTAGCCGCACAATAA

Upstream 100 bases:

>100_bases
AGTCGTCAAAATGTTGTTATAAATTATTTGATTTATCACGGATAACATAGATTTGTATAAAACTTTGTTATCCGTCTTTT
TTATTTACAAAGGAATAAAA

Downstream 100 bases:

>100_bases
GTGCTAATTTCTTCAAATATGGAAGATGATAAAATTTCAGTCACTCGGCATTTGCAAAGTTTGCTACAAATTTAACCGCT
TATCTCTTCCGTATTTTCTC

Product: 23S rRNA (guanosine-2'-O-)-methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MSEQIYGIHAVKAFLDNAPERLIEVLVLKGREDKRLTPLLNELQRLGISVQQVNRQTLDNKAQGEVHQGIIAKVVPQKEL
NEHDLDAILAQKQNPFLLILDGVTDPHNLGACLRTADAAGVDAVIVPKDKSAQLTSTARKVACGAAETVPLIRVTNLART
MRELQEQHNIWIVGTAGEATSGIYQAKLTGAIALVMGAEGDGMRRLTREHCDQLISIPMAGSVSSLNVSVATGVCLFEIV
RQKLAAQ

Sequences:

>Translated_247_residues
MSEQIYGIHAVKAFLDNAPERLIEVLVLKGREDKRLTPLLNELQRLGISVQQVNRQTLDNKAQGEVHQGIIAKVVPQKEL
NEHDLDAILAQKQNPFLLILDGVTDPHNLGACLRTADAAGVDAVIVPKDKSAQLTSTARKVACGAAETVPLIRVTNLART
MRELQEQHNIWIVGTAGEATSGIYQAKLTGAIALVMGAEGDGMRRLTREHCDQLISIPMAGSVSSLNVSVATGVCLFEIV
RQKLAAQ
>Mature_246_residues
SEQIYGIHAVKAFLDNAPERLIEVLVLKGREDKRLTPLLNELQRLGISVQQVNRQTLDNKAQGEVHQGIIAKVVPQKELN
EHDLDAILAQKQNPFLLILDGVTDPHNLGACLRTADAAGVDAVIVPKDKSAQLTSTARKVACGAAETVPLIRVTNLARTM
RELQEQHNIWIVGTAGEATSGIYQAKLTGAIALVMGAEGDGMRRLTREHCDQLISIPMAGSVSSLNVSVATGVCLFEIVR
QKLAAQ

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=213, Percent_Identity=30.5164319248826, Blast_Score=86, Evalue=3e-17,
Organism=Escherichia coli, GI1790623, Length=243, Percent_Identity=73.2510288065844, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI1788935, Length=247, Percent_Identity=27.5303643724696, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1790083, Length=158, Percent_Identity=29.746835443038, Blast_Score=61, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17555908, Length=262, Percent_Identity=27.4809160305344, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6324775, Length=289, Percent_Identity=25.2595155709343, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26692; Mature: 26561

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQIYGIHAVKAFLDNAPERLIEVLVLKGREDKRLTPLLNELQRLGISVQQVNRQTLDN
CCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCC
KAQGEVHQGIIAKVVPQKELNEHDLDAILAQKQNPFLLILDGVTDPHNLGACLRTADAAG
CCCCHHHHHHHHHHCCHHHCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCC
VDAVIVPKDKSAQLTSTARKVACGAAETVPLIRVTNLARTMRELQEQHNIWIVGTAGEAT
CCEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCC
SGIYQAKLTGAIALVMGAEGDGMRRLTREHCDQLISIPMAGSVSSLNVSVATGVCLFEIV
CCCHHHHHHCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHH
RQKLAAQ
HHHHCCC
>Mature Secondary Structure 
SEQIYGIHAVKAFLDNAPERLIEVLVLKGREDKRLTPLLNELQRLGISVQQVNRQTLDN
CCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCC
KAQGEVHQGIIAKVVPQKELNEHDLDAILAQKQNPFLLILDGVTDPHNLGACLRTADAAG
CCCCHHHHHHHHHHCCHHHCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCC
VDAVIVPKDKSAQLTSTARKVACGAAETVPLIRVTNLARTMRELQEQHNIWIVGTAGEAT
CCEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCC
SGIYQAKLTGAIALVMGAEGDGMRRLTREHCDQLISIPMAGSVSSLNVSVATGVCLFEIV
CCCHHHHHHCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHH
RQKLAAQ
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA