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The map label for this gene is gsiD [H]

Identifier: 126207515

GI number: 126207515

Start: 32004

End: 32789

Strand: Reverse

Name: gsiD [H]

Synonym: APL_0027

Alternate gene names: 126207515

Gene position: 32789-32004 (Counterclockwise)

Preceding gene: 126207516

Following gene: 126207514

Centisome position: 1.44

GC content: 43.0

Gene sequence:

>786_bases
ATGAATAAAATGACATTTAGCCAAAAAATAGGGGCGATAATTCTTACTATATTACTCGCTTTTGCCTTTTTACAGCCTTA
TTTTTACCCGATGGATATTGGCTTCCAAGATCTTGGTAATATGCTTGCTAAACCGGATGAGTTTGCTTGGCTCGGCACCG
ATCATCTCGGCAGAGATATGCTTGCTCGCCTTGCTTCGGCGATTCGTTTATCATTCGGTCTATCTCTTTTTAGCGTATTT
TGTGCCTTAGTTGCCGGGCTGCTATTTGGTATTCTAGCCGGCTTTTTCGGCGGATGGCTTGACCGACTATTCAGCTTTAT
CTGCGATTTGGTGATGGCGTTACCCGGATTATTATTGATTCTACTCTTTGCCTCATTATCACCGGGATCATTTTGGACGC
TCTATTTAGGTATCGCTTTAGTGATGTGGGTGGAATTTTTCCGTGTGATTCGAGCAGTCAGCCAAACACTTGCTTCCAGT
GCCGAAATCGAATCTTCACGTTTAATGGGAATGGGCTTATTTTACAGTTTTAAACGCCATCTTTTACCGCGTTTACTGCC
GCTTCTTGTGACCTTAAGTGCTTTCTCATTAGGTAATGCGATTCTGGCGTTAGCCACTCTCGGATTTGTCAATGTCGGGC
TACGTCCGCCAACAGCAGAATTAGGTTTAATGATGACCGAATTATTCCCTTACTACTATGAAGCCCCTTGGATCTTTATG
CAGCCGGTAGTTGCGGTCTTTTTCATGGTACTTAGTCTTCAACTTTTATCTGGGAGAGTAAAATAA

Upstream 100 bases:

>100_bases
TCGTAATGGGCGGTCTATTTGTCCTGTTAAATATGTGTGCGGATATGCTAAGTGCGTGGATTGACCCGCGAATTACCCAT
AAACGTCATGGAGAATAACA

Downstream 100 bases:

>100_bases
TGGCACTGCTACGAATTGAAAATGTTGGGGTAAAAACCACTACAGGTTTAACTTTAGTTGAACCGATCAGTTTAAGTTTA
GAACAGGGCAAAAATATCAC

Product: nickel transport system permease protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MNKMTFSQKIGAIILTILLAFAFLQPYFYPMDIGFQDLGNMLAKPDEFAWLGTDHLGRDMLARLASAIRLSFGLSLFSVF
CALVAGLLFGILAGFFGGWLDRLFSFICDLVMALPGLLLILLFASLSPGSFWTLYLGIALVMWVEFFRVIRAVSQTLASS
AEIESSRLMGMGLFYSFKRHLLPRLLPLLVTLSAFSLGNAILALATLGFVNVGLRPPTAELGLMMTELFPYYYEAPWIFM
QPVVAVFFMVLSLQLLSGRVK

Sequences:

>Translated_261_residues
MNKMTFSQKIGAIILTILLAFAFLQPYFYPMDIGFQDLGNMLAKPDEFAWLGTDHLGRDMLARLASAIRLSFGLSLFSVF
CALVAGLLFGILAGFFGGWLDRLFSFICDLVMALPGLLLILLFASLSPGSFWTLYLGIALVMWVEFFRVIRAVSQTLASS
AEIESSRLMGMGLFYSFKRHLLPRLLPLLVTLSAFSLGNAILALATLGFVNVGLRPPTAELGLMMTELFPYYYEAPWIFM
QPVVAVFFMVLSLQLLSGRVK
>Mature_261_residues
MNKMTFSQKIGAIILTILLAFAFLQPYFYPMDIGFQDLGNMLAKPDEFAWLGTDHLGRDMLARLASAIRLSFGLSLFSVF
CALVAGLLFGILAGFFGGWLDRLFSFICDLVMALPGLLLILLFASLSPGSFWTLYLGIALVMWVEFFRVIRAVSQTLASS
AEIESSRLMGMGLFYSFKRHLLPRLLPLLVTLSAFSLGNAILALATLGFVNVGLRPPTAELGLMMTELFPYYYEAPWIFM
QPVVAVFFMVLSLQLLSGRVK

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1173

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787054, Length=247, Percent_Identity=30.7692307692308, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1789889, Length=235, Percent_Identity=30.6382978723404, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1787760, Length=251, Percent_Identity=28.2868525896414, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1789964, Length=206, Percent_Identity=32.0388349514563, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1787549, Length=241, Percent_Identity=29.4605809128631, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1788505, Length=204, Percent_Identity=28.921568627451, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787498, Length=232, Percent_Identity=24.1379310344828, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 28987; Mature: 28987

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA