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The map label for this gene is trmE [H]

Identifier: 126176560

GI number: 126176560

Start: 5123121

End: 5124560

Strand: Reverse

Name: trmE [H]

Synonym: Sbal_4379

Alternate gene names: 126176560

Gene position: 5124560-5123121 (Counterclockwise)

Preceding gene: 126176561

Following gene: 126176559

Centisome position: 99.95

GC content: 47.43

Gene sequence:

>1440_bases
ATGACACTAAAAGGCGGCTTAATTGGCCGCCTTTTCCTTTACATATTTTATGTAGATATTAGTTTGGTGGGTATTTCCGT
GACAACTGACACTATCGTGGCTCAAGCCACCGCGCCCGGACGTGGCGGCGTTGGCATCATTCGAATTTCAGGCGACAAGG
CCAGTAATGTCGCTATGGCCGTATTAGGTCATCTTCCTAAAACCCGATATGCCGATTATTGCGATTTTAAAAGTGCATCG
GGCCAAGTAATAGACCAAGGCATAGCGCTGTTTTTTAAAGGACCGAATTCCTTTACTGGCGAAGATGTCTTGGAACTTCA
AGGCCACGGCGGCCAAATTGTCCTCGACATGCTGATCAAACGTGTAATGGAAGTCGGTGGTATTCGTATAGCAAAACCAG
GCGAGTTCAGCGAACAAGCCTTTATGAACGATAAACTCGACTTAACCCAAGCCGAAGCCATTGCCGACCTTATCGATGCC
ACCTGTGAGCAAGCCGCTAAAAGCGCGCTGCAATCACTACAAGGTGAATTTTCTAAGGAAGTCCACGAGCTGGTGGACCA
AGTCACTAACCTGCGTTTATACGTTGAAGCCGCAATTGATTTCCCCGATGAAGAAGTCGACTTTTTAAGTGATGGCAAAA
TTGCTAATGCACTCTATAAAATCATCGACAAACTTGACCTAGTGCAAGCCAGTGCCAAGCAAGGTTCTATTATTCGTGAA
GGGATGAAAGTGGTGATTGCCGGTCGTCCCAATGCCGGAAAATCGAGCTTACTGAATGCGTTAGCGGGTAAAGAGTCGGC
CATTGTGACTGAAATTGCCGGTACAACACGCGACGTATTACGTGAACATATCCATTTAGATGGTATGCCGCTGCACATTA
TTGATACCGCTGGCCTGCGTGATACCACAGATACGGTAGAACAAATTGGTATTGAACGCGCATGGAATGAAATAAACAGC
GCCGATCGCGTGCTATTTATGGTGGACGGCACGACAACCGCCGCCGTCGATCCCCATGCGATTTGGCCTGACTTTGTCGA
TCGCCTGCCCTCCAATTTAGGTGTAACAGTGATCCGTAATAAAGCCGATCTCACGGGCGAAGACTTAATGATGACCGAAG
AACAAGGTTATAGCGTCTACCGGATCTCGGCTAAAACAGGCTTAGGTGTTGAAGAATTAAAGCAACACTTGAAGTCACTT
ATGGGTTATCAGAGTAATCTCGAAGGTGGCTTTATCGCCCGTCGTCGTCACTTAGAAGCCCTTGAATTAGCCGCAGGACA
CCTGCAATTAGGTAAAGAGCAGTTAGAGGTTTACCTTGCCGGAGAGCTGCTAGCGGAAGAGCTACGCATGTGCCAATTAG
CACTATCAGAGATCACTGGTCGCTTCACTTCTGACGACCTACTCGGCAAGATTTTCAGCTCTTTCTGTATTGGGAAGTAA

Upstream 100 bases:

>100_bases
CGCAACAGAAGATTATTTATGCAGGTCTGGCGAAAAAAGGCTTAAAATAAGCCTAAGATTTGCAAGACTCTAGCGCATCG
TTACAGATTAATGTGAACGA

Downstream 100 bases:

>100_bases
TGTTCTCGCCATGTGAACTGCTTTTCTAGCTAAGGCTTGCTGTGCTCAACAAGCCTTTCTTTTAACTAAAACCTCGTATT
CTTGCTTATATTCCTCCCTA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MTLKGGLIGRLFLYIFYVDISLVGISVTTDTIVAQATAPGRGGVGIIRISGDKASNVAMAVLGHLPKTRYADYCDFKSAS
GQVIDQGIALFFKGPNSFTGEDVLELQGHGGQIVLDMLIKRVMEVGGIRIAKPGEFSEQAFMNDKLDLTQAEAIADLIDA
TCEQAAKSALQSLQGEFSKEVHELVDQVTNLRLYVEAAIDFPDEEVDFLSDGKIANALYKIIDKLDLVQASAKQGSIIRE
GMKVVIAGRPNAGKSSLLNALAGKESAIVTEIAGTTRDVLREHIHLDGMPLHIIDTAGLRDTTDTVEQIGIERAWNEINS
ADRVLFMVDGTTTAAVDPHAIWPDFVDRLPSNLGVTVIRNKADLTGEDLMMTEEQGYSVYRISAKTGLGVEELKQHLKSL
MGYQSNLEGGFIARRRHLEALELAAGHLQLGKEQLEVYLAGELLAEELRMCQLALSEITGRFTSDDLLGKIFSSFCIGK

Sequences:

>Translated_479_residues
MTLKGGLIGRLFLYIFYVDISLVGISVTTDTIVAQATAPGRGGVGIIRISGDKASNVAMAVLGHLPKTRYADYCDFKSAS
GQVIDQGIALFFKGPNSFTGEDVLELQGHGGQIVLDMLIKRVMEVGGIRIAKPGEFSEQAFMNDKLDLTQAEAIADLIDA
TCEQAAKSALQSLQGEFSKEVHELVDQVTNLRLYVEAAIDFPDEEVDFLSDGKIANALYKIIDKLDLVQASAKQGSIIRE
GMKVVIAGRPNAGKSSLLNALAGKESAIVTEIAGTTRDVLREHIHLDGMPLHIIDTAGLRDTTDTVEQIGIERAWNEINS
ADRVLFMVDGTTTAAVDPHAIWPDFVDRLPSNLGVTVIRNKADLTGEDLMMTEEQGYSVYRISAKTGLGVEELKQHLKSL
MGYQSNLEGGFIARRRHLEALELAAGHLQLGKEQLEVYLAGELLAEELRMCQLALSEITGRFTSDDLLGKIFSSFCIGK
>Mature_478_residues
TLKGGLIGRLFLYIFYVDISLVGISVTTDTIVAQATAPGRGGVGIIRISGDKASNVAMAVLGHLPKTRYADYCDFKSASG
QVIDQGIALFFKGPNSFTGEDVLELQGHGGQIVLDMLIKRVMEVGGIRIAKPGEFSEQAFMNDKLDLTQAEAIADLIDAT
CEQAAKSALQSLQGEFSKEVHELVDQVTNLRLYVEAAIDFPDEEVDFLSDGKIANALYKIIDKLDLVQASAKQGSIIREG
MKVVIAGRPNAGKSSLLNALAGKESAIVTEIAGTTRDVLREHIHLDGMPLHIIDTAGLRDTTDTVEQIGIERAWNEINSA
DRVLFMVDGTTTAAVDPHAIWPDFVDRLPSNLGVTVIRNKADLTGEDLMMTEEQGYSVYRISAKTGLGVEELKQHLKSLM
GYQSNLEGGFIARRRHLEALELAAGHLQLGKEQLEVYLAGELLAEELRMCQLALSEITGRFTSDDLLGKIFSSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=470, Percent_Identity=34.0425531914894, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI193082993, Length=460, Percent_Identity=34.1304347826087, Blast_Score=209, Evalue=7e-54,
Organism=Homo sapiens, GI193082988, Length=502, Percent_Identity=31.8725099601594, Blast_Score=204, Evalue=2e-52,
Organism=Escherichia coli, GI2367268, Length=451, Percent_Identity=72.5055432372506, Blast_Score=674, Evalue=0.0,
Organism=Escherichia coli, GI87082120, Length=92, Percent_Identity=38.0434782608696, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17507259, Length=469, Percent_Identity=29.2110874200426, Blast_Score=158, Evalue=7e-39,
Organism=Saccharomyces cerevisiae, GI6323665, Length=497, Percent_Identity=31.1871227364185, Blast_Score=202, Evalue=1e-52,
Organism=Drosophila melanogaster, GI45550813, Length=474, Percent_Identity=32.7004219409283, Blast_Score=213, Evalue=2e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 51985; Mature: 51854

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKGGLIGRLFLYIFYVDISLVGISVTTDTIVAQATAPGRGGVGIIRISGDKASNVAMA
CCCCCHHHHHHHHHHHHHHHHHEEEEEECCCEEEEECCCCCCCEEEEEECCCCCHHHHHH
VLGHLPKTRYADYCDFKSASGQVIDQGIALFFKGPNSFTGEDVLELQGHGGQIVLDMLIK
HHHCCCCCCCCCCCCCCCCCCHHHHCCEEEEEECCCCCCCCCCEEECCCCCEEHHHHHHH
RVMEVGGIRIAKPGEFSEQAFMNDKLDLTQAEAIADLIDATCEQAAKSALQSLQGEFSKE
HHHHHCCEEEECCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VHELVDQVTNLRLYVEAAIDFPDEEVDFLSDGKIANALYKIIDKLDLVQASAKQGSIIRE
HHHHHHHHHCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHC
GMKVVIAGRPNAGKSSLLNALAGKESAIVTEIAGTTRDVLREHIHLDGMPLHIIDTAGLR
CCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCCC
DTTDTVEQIGIERAWNEINSADRVLFMVDGTTTAAVDPHAIWPDFVDRLPSNLGVTVIRN
CHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEECCCCCCCCHHHHHHCCCCCCEEEEEC
KADLTGEDLMMTEEQGYSVYRISAKTGLGVEELKQHLKSLMGYQSNLEGGFIARRRHLEA
CCCCCCCCEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
LELAAGHLQLGKEQLEVYLAGELLAEELRMCQLALSEITGRFTSDDLLGKIFSSFCIGK
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLKGGLIGRLFLYIFYVDISLVGISVTTDTIVAQATAPGRGGVGIIRISGDKASNVAMA
CCCCHHHHHHHHHHHHHHHHHEEEEEECCCEEEEECCCCCCCEEEEEECCCCCHHHHHH
VLGHLPKTRYADYCDFKSASGQVIDQGIALFFKGPNSFTGEDVLELQGHGGQIVLDMLIK
HHHCCCCCCCCCCCCCCCCCCHHHHCCEEEEEECCCCCCCCCCEEECCCCCEEHHHHHHH
RVMEVGGIRIAKPGEFSEQAFMNDKLDLTQAEAIADLIDATCEQAAKSALQSLQGEFSKE
HHHHHCCEEEECCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VHELVDQVTNLRLYVEAAIDFPDEEVDFLSDGKIANALYKIIDKLDLVQASAKQGSIIRE
HHHHHHHHHCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHC
GMKVVIAGRPNAGKSSLLNALAGKESAIVTEIAGTTRDVLREHIHLDGMPLHIIDTAGLR
CCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCCC
DTTDTVEQIGIERAWNEINSADRVLFMVDGTTTAAVDPHAIWPDFVDRLPSNLGVTVIRN
CHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEECCCCCCCCHHHHHHCCCCCCEEEEEC
KADLTGEDLMMTEEQGYSVYRISAKTGLGVEELKQHLKSLMGYQSNLEGGFIARRRHLEA
CCCCCCCCEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
LELAAGHLQLGKEQLEVYLAGELLAEELRMCQLALSEITGRFTSDDLLGKIFSSFCIGK
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA